Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,657,440 T→C 6.2% K499R (AAA→AGA)  hscA ← DnaK‑like molecular chaperone specific for IscU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,657,4400TC 6.2% 288.0 / 7.3 97K499R (AAA→AGA) hscADnaK‑like molecular chaperone specific for IscU
Reads supporting (aligned to +/- strand):  ref base T (50/41);  new base C (3/3);  total (53/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 103 total aligned reads displayed.

CAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAG  >  NC_000913/2657291‑2657589
                                                                                                                                                     |                                                                                                                                                     
cagccagcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGtt                                                                                                                                                       >  2:817684/1‑150 (MQ=255)
cagccagcGCGCCGGGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGtt                                                                                                                                                       <  2:933644/150‑1 (MQ=255)
 agccagcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGttt                                                                                                                                                      <  2:100558/150‑1 (MQ=255)
 agccagcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGttt                                                                                                                                                      <  1:1283783/150‑1 (MQ=255)
 agccagcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGATCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGttt                                                                                                                                                      <  1:1490920/150‑1 (MQ=255)
  gccagcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTg                                                                                                                                                     <  2:938316/150‑1 (MQ=255)
  gccagcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTg                                                                                                                                                     <  1:811187/150‑1 (MQ=255)
  gccagcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTg                                                                                                                                                     >  1:661110/1‑150 (MQ=255)
    cagcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGGTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGAc                                                                                                                                                   <  2:236863/150‑1 (MQ=255)
     agcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGAcc                                                                                                                                                  <  2:1455952/150‑1 (MQ=255)
     agcGCGCCGGGCAGAGTTTGCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGGTCGGCATAGCTCTTTGAGTCTTTGATCATCGAAGCGATATCGCTATCGGTCAAACCGTCTTACGGTCTGaac                                                                                                                                                  <  1:261808/150‑3 (MQ=255)
       cgcgcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTg                                                                                                                                                <  2:1261589/150‑1 (MQ=255)
       cgcgcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTg                                                                                                                                                <  1:60866/150‑1 (MQ=255)
          gcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAAt                                                                                                                                             >  1:1666055/1‑150 (MQ=255)
          gcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAAACCGTCTTACGGTCTGAccgatag                                                                                                                                             <  2:492017/150‑6 (MQ=255)
           ccGTGCAGACTTTCCCGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATa                                                                                                                                            >  1:1667078/1‑150 (MQ=255)
            cGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAg                                                                                                                                           <  1:1636119/150‑1 (MQ=255)
               gCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACg                                                                                                                                        >  1:1091660/1‑150 (MQ=255)
                  gACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCt                                                                                                                                     <  1:703558/150‑1 (MQ=255)
                      ttCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAAc                                                                                                                                 >  1:386092/1‑150 (MQ=255)
                      ttCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAAc                                                                                                                                 <  1:1216938/150‑1 (MQ=255)
                        ccAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGc                                                                                                                               >  2:1681351/1‑150 (MQ=255)
                         cAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGcc                                                                                                                              >  2:888211/1‑150 (MQ=255)
                           gCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCTCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCgg                                                                                                                            >  1:1296725/1‑150 (MQ=255)
                              cacGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAGCGCCGGTgg                                                                                                                         >  2:1210516/1‑150 (MQ=255)
                                 gcgcCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGAtt                                                                                                                      <  1:1606469/150‑1 (MQ=255)
                                 gcgcCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGAtt                                                                                                                      <  2:60441/150‑1 (MQ=255)
                                 gcgcCGCTTCTACTTTATGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGAtt                                                                                                                      <  2:862265/150‑1 (MQ=255)
                                     cgcTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTtctc                                                                                                                  <  2:754882/150‑1 (MQ=255)
                                          cTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCg                                                                                                             >  2:1411931/1‑150 (MQ=255)
                                              tttttGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGt                                                                                                         >  1:1550452/1‑150 (MQ=255)
                                               ttttGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTc                                                                                                        >  2:1651111/1‑150 (MQ=255)
                                               ttttGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCCATTTCGCTATCGGTCAGACCTTAAGCCGGTTTGAACTCAATAGACGCCTCAACGCCGGTGGATTTCTCAATCGCCGTc                                                                                                        >  1:1285913/1‑150 (MQ=255)
                                                   gTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGc                                                                                                    >  2:321404/1‑150 (MQ=255)
                                                    ttCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCt                                                                                                   <  2:1485979/150‑1 (MQ=255)
                                                    ttCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCt                                                                                                   <  1:160727/150‑1 (MQ=255)
                                                     tCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTc                                                                                                  <  1:1720293/150‑1 (MQ=255)
                                                      cTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCa                                                                                                 <  2:511995/150‑1 (MQ=255)
                                                         ccAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCaaaa                                                                                              >  2:783471/1‑150 (MQ=255)
                                                          cAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAg                                                                                             <  2:777728/150‑1 (MQ=255)
                                                          cAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAg                                                                                             <  2:1534058/150‑1 (MQ=255)
                                                          cAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAg                                                                                             <  1:934550/150‑1 (MQ=255)
                                                             cATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGAcc                                                                                          >  1:566272/1‑150 (MQ=255)
                                                                    gCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCa                                                                                   >  1:714836/1‑150 (MQ=255)
                                                                     cTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCAt                                                                                  >  1:619463/1‑150 (MQ=255)
                                                                      ttttACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATc                                                                                 <  2:1358873/150‑1 (MQ=255)
                                                                       tttACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCg                                                                                <  2:1091660/150‑1 (MQ=255)
                                                                          aCGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGAcc                                                                             <  1:1042194/150‑1 (MQ=255)
                                                                              ccTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGa                                                                         >  1:1538483/1‑150 (MQ=255)
                                                                                tGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCTTCGACCTGGAAc                                                                       >  2:51841/1‑150 (MQ=255)
                                                                                tGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAAc                                                                       >  1:440318/1‑150 (MQ=255)
                                                                                 gCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACg                                                                      >  1:322800/1‑150 (MQ=255)
                                                                                 gCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACg                                                                      >  2:36783/1‑150 (MQ=255)
                                                                                   gcGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTc                                                                    <  2:1434407/149‑1 (MQ=255)
                                                                                      gCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGGATCGACCTGGAACGTCAcg                                                                 >  2:54906/1‑150 (MQ=255)
                                                                                      gCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGCCCTGGAACGTCAcg                                                                 >  2:800393/1‑150 (MQ=255)
                                                                                      gCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCAcg                                                                 >  2:651714/1‑150 (MQ=255)
                                                                                       cATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCAcgc                                                                <  1:556254/150‑1 (MQ=255)
                                                                                           gCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCAGGAACGTCACGCGaaa                                                            >  2:605858/1‑149 (MQ=255)
                                                                                             tCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGCCCTGGAACGTCACGCGAataa                                                          >  1:1622817/1‑149 (MQ=255)
                                                                                             tCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAatat                                                          <  1:1402816/150‑1 (MQ=255)
                                                                                               agtGAGTCTTTGATCTTCGAAGCGATGTCGCTTTCGGTCTGAGCGTATGACGGTTTGTCCTGAATAGACGCCTCAACGCCGGTGGATGTCTGGATCGCGGTCACGCTCAAAAGTCCGTCGGCAGCGTCCTGGAACTTCACGGGAATATgc                                                        <  2:1505788/148‑1 (MQ=255)
                                                                                               aTTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATgc                                                        >  2:978131/1‑150 (MQ=255)
                                                                                                  gAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGcaca                                                     >  1:1542647/1‑150 (MQ=255)
                                                                                                   aGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGCCCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCAc                                                    >  2:1510952/1‑150 (MQ=255)
                                                                                                      cTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGc                                                 >  1:1670739/1‑150 (MQ=255)
                                                                                                       tttGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGCCCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGCCCTGGAACGTCACGCGAATATGCGCACCGcc                                                >  2:991722/1‑150 (MQ=255)
                                                                                                       tttGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGcc                                                <  2:714836/150‑1 (MQ=255)
                                                                                                          gATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGc                                             >  2:773434/1‑150 (MQ=255)
                                                                                                            tcatcGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCg                                           >  2:699766/1‑150 (MQ=255)
                                                                                                                cGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAg                                       <  2:1667078/150‑1 (MQ=255)
                                                                                                                cGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAg                                       >  1:979677/1‑150 (MQ=255)
                                                                                                                cGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAg                                       >  2:420616/1‑150 (MQ=255)
                                                                                                                cGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGGCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAg                                       <  1:1008588/150‑1 (MQ=255)
                                                                                                                 gAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAgc                                      >  1:1694037/1‑150 (MQ=255)
                                                                                                                     cGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCg                                  <  2:580679/150‑1 (MQ=255)
                                                                                                                     cGATTTCGCTATCGGTCAGACCGTAAGACGGTTAGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAATAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCg                                  <  1:783158/150‑1 (MQ=255)
                                                                                                                            tattcaggTCAAACCGTCTTACGGTCTGACCTGAATAGACGCCTCAATGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATAcc                           <  1:965855/144‑1 (MQ=255)
                                                                                                                            gCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATAcc                           >  1:159148/1‑150 (MQ=255)
                                                                                                                             cTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCa                          <  2:386092/150‑1 (MQ=255)
                                                                                                                             cTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGAGTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCa                          <  2:322800/150‑1 (MQ=255)
                                                                                                                             attcaggTCAAACCGTCTTACGGTCTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCa                          >  1:1259180/6‑150 (MQ=255)
                                                                                                                              tATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCAc                         <  1:817684/150‑1 (MQ=255)
                                                                                                                              tATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTACTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAAGACCAc                         >  1:280655/1‑150 (MQ=255)
                                                                                                                               aTCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACg                        <  2:796221/150‑1 (MQ=255)
                                                                                                                                tCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGc                       >  1:961366/1‑150 (MQ=255)
                                                                                                                                tCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGc                       <  1:1727206/150‑1 (MQ=255)
                                                                                                                                  ggTCAAACCGTCTTACGGTCTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAg                     >  2:1602759/1‑150 (MQ=255)
                                                                                                                                  ggTCAAACCGTCTTACGGTCTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAg                     >  2:1020524/1‑150 (MQ=255)
                                                                                                                                   gTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGAACTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCAcgccgc                    >  2:1216646/1‑150 (MQ=255)
                                                                                                                                       gACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTGAATAGACCGTCGGCATCGACCGGGAACGTCACGCGAATATGCGCACCGACAGCCGGTAGCGCCGGCATCCCACGCAGCGCaa                >  1:239979/1‑150 (MQ=255)
                                                                                                                                         ccGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCaaaa              >  1:494677/1‑150 (MQ=255)
                                                                                                                                         ccGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCaaaa              <  1:1563533/150‑1 (MQ=255)
                                                                                                                                             aaGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATCCCACGCAGCGCAAAAcgcg          >  1:1477939/1‑150 (MQ=255)
                                                                                                                                               gACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCCCGCAGCGCAAAACGCGcc        >  1:1157533/1‑150 (MQ=255)
                                                                                                                                               gACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGcc        >  1:1321567/1‑150 (MQ=255)
                                                                                                                                               gACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTACTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGcc        <  2:280655/150‑1 (MQ=255)
                                                                                                                                                   gTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCTAAACGCGCCAGTg    >  1:231106/1‑150 (MQ=255)
                                                                                                                                                   gTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGAAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTg    >  1:922451/1‑150 (MQ=255)
                                                                                                                                                     ttGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAg  <  1:978131/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAG  >  NC_000913/2657291‑2657589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: