BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	312,304	A→T	10.0%	I65L (ATA→TTA) ‡	ykgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	312,304	0	A	T	10.0%	355.6 / 25.0	140	I65L (ATA→TTA) ‡	ykgL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (65/61); new base T (6/8); total (71/69)
Fisher's exact test for biased strand distribution p-value = 5.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.64e-01

Only 100 of 149 total aligned reads displayed.

GTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCT  >  NC_000913/312156‑312453

gtattaaaGAGGGTTGATGCCTGCATACAGGGGCGAAGCTGGGAAACAAGTCAACATTAAGTTAATGGAATATTCGGAGAGTAACGGCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCAAGGAAAATTAATGTTGGTGTtat                                                                                                                                                      <  2:1121656/145‑1 (MQ=255)

gTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTtat                                                                                                                                                      >  1:119454/1‑150 (MQ=255)

  cTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATaa                                                                                                                                                    <  2:622075/150‑1 (MQ=255)

  cTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAAGGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCACAGGAAAATTAATGTTGGTGTTATaa                                                                                                                                                    >  1:1591778/1‑150 (MQ=255)

      aaGAGGGTTGATGCCAGCATACAGGGGCGAAGCTTGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCATGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacac                                                                                                                                                <  1:193817/150‑1 (MQ=255)

      aaGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacac                                                                                                                                                >  2:947340/1‑150 (MQ=255)

      aaGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacac                                                                                                                                                <  2:446562/150‑1 (MQ=255)

       agagGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCACTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTTTTATAAaccca                                                                                                                                               >  2:833757/1‑150 (MQ=255)

            gTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAAc                                                                                                                                          <  1:617109/150‑1 (MQ=255)

             ttGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATTCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACa                                                                                                                                         <  2:411938/150‑1 (MQ=255)

             ttGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACa                                                                                                                                         <  2:738901/150‑1 (MQ=255)

              tGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACAt                                                                                                                                        <  1:1479578/150‑1 (MQ=255)

              tGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACAt                                                                                                                                        <  1:990945/150‑1 (MQ=255)

              tGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACAt                                                                                                                                        <  1:833952/150‑1 (MQ=255)

              tGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACAt                                                                                                                                        <  2:127763/150‑1 (MQ=255)

               gATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACAtt                                                                                                                                       >  1:651437/1‑150 (MQ=255)

                aTGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTa                                                                                                                                      >  2:1474283/1‑150 (MQ=255)

                   ccAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACATTAAtt                                                                                                                                   <  2:399149/150‑2 (MQ=39)

                    cAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGTCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATcc                                                                                                                                  <  1:1167145/150‑1 (MQ=255)

                    cAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATcc                                                                                                                                  <  1:574802/150‑1 (MQ=255)

                      gCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGa                                                                                                                                >  2:728182/1‑150 (MQ=255)

                         tACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCt                                                                                                                             <  1:243/150‑1 (MQ=255)

                         tACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACACTAATCCGATCt                                                                                                                             <  2:1269030/150‑1 (MQ=255)

                          aCAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTg                                                                                                                            >  2:1380968/1‑150 (MQ=255)

                             tgggCGAAGCGGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATTTATCCCCAGTAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGatt                                                                                                                         <  2:1054223/149‑1 (MQ=255)

                              gggCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGatta                                                                                                                        >  2:1099660/1‑150 (MQ=255)

                                gCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGATGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGattatt                                                                                                                      >  2:445894/1‑150 (MQ=255)

                                gCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGattatt                                                                                                                      <  2:208769/150‑1 (MQ=255)

                                 cGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGAttatta                                                                                                                     >  1:680702/1‑150 (MQ=255)

                                        ggTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGAc                                                                                                              >  2:860075/1‑150 (MQ=255)

                                        ggTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGAc                                                                                                              >  1:171126/1‑150 (MQ=255)

                                           caacaaGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACaaa                                                                                                           >  1:730930/1‑150 (MQ=255)

                                           caacaaGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAAAGAACAGCAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTCATTATTAAACAGACaaa                                                                                                           >  2:591323/1‑150 (MQ=255)

                                            aacaaGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAat                                                                                                          >  1:1141799/1‑150 (MQ=255)

                                             acaaGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAata                                                                                                         <  2:380104/150‑1 (MQ=255)

                                             acaaGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAata                                                                                                         >  2:509551/1‑150 (MQ=255)

                                                 gTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGt                                                                                                     <  2:550647/150‑1 (MQ=255)

                                                  tCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGtt                                                                                                    <  2:286504/150‑1 (MQ=255)

                                                  tCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGtt                                                                                                    <  1:1624431/150‑1 (MQ=255)

                                                   cAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTa                                                                                                   <  1:642866/150‑1 (MQ=255)

                                                     aCATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAAt                                                                                                 >  2:919232/1‑150 (MQ=255)

                                                           aGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCg                                                                                           <  2:1231118/150‑1 (MQ=255)

                                                                 tGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAAtttt                                                                                     >  1:664907/1‑150 (MQ=255)

                                                                   gAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGa                                                                                   >  2:1253848/1‑150 (MQ=255)

                                                                   gAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGa                                                                                   <  1:1241227/150‑1 (MQ=255)

                                                                    aaTATTCGGAGAGTAACGTCTGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCTTGAAAATTAATGTTGGTGTTATAAACACAGCATCATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGaa                                                                                  <  1:9838/150‑1 (MQ=255)

                                                                           ggAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGt                                                                           >  2:322286/1‑150 (MQ=255)

                                                                               agaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTTAATCAACGttttt                                                                       <  1:1200206/150‑1 (MQ=255)

                                                                               agaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGttttt                                                                       >  2:1660883/1‑150 (MQ=255)

                                                                                taaaCGTCATGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGtttttt                                                                      <  1:357498/149‑1 (MQ=255)

                                                                                 aaaCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTc                                                                     <  1:14214/150‑1 (MQ=255)

                                                                                  aaCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCt                                                                    <  1:1405846/150‑1 (MQ=255)

                                                                                   aCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCtt                                                                   >  2:1511221/1‑150 (MQ=255)

                                                                                   aCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCtt                                                                   <  2:174878/150‑1 (MQ=255)

                                                                                    cGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTa                                                                  <  1:930239/150‑1 (MQ=255)

                                                                                     gTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTAc                                                                 <  1:509551/150‑1 (MQ=255)

                                                                                          gCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGAccc                                                            <  2:243419/150‑1 (MQ=255)

                                                                                             gTTGGCCTCAAATGAACATGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATACGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCTAATTTTGAATCAACGTTTTTTCTTACTACACTGa                                                         >  2:19551/1‑150 (MQ=255)

                                                                                                 gCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACgg                                                     >  2:777544/1‑150 (MQ=255)

                                                                                                  ccTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACCCCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGc                                                    >  1:1536849/1‑150 (MQ=255)

                                                                                                   cTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGcc                                                   >  2:1149019/1‑150 (MQ=255)

                                                                                                   cTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGcc                                                   >  1:555751/1‑150 (MQ=255)

                                                                                                   cTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGcc                                                   <  1:860075/150‑1 (MQ=255)

                                                                                                    tCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAAAACACCAAAATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGACCGGCCt                                                  <  1:84912/150‑1 (MQ=255)

                                                                                                           aaCAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCAGTCGAATTTTGAAACAAAGTTTTTTCTTAAGACCCTGAACGGCCTTAAAAct                                           >  2:237559/1‑149 (MQ=255)

                                                                                                            aCAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGt                                          >  1:167765/1‑150 (MQ=255)

                                                                                                             cAGGAAGAATATATCCCCATGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTg                                         <  1:1544588/150‑1 (MQ=255)

                                                                                                             cAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTg                                         <  1:753234/150‑1 (MQ=255)

                                                                                                              aGGAAGAATATATCCCCAGGAATATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTTATCAGACATATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAATCGTgg                                        <  1:605024/150‑1 (MQ=255)

                                                                                                              aGGAAGAATATATACCCATGAAAATTAATGTTGGTTTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTATCTTACGACCCTGAACGGCCTTAAAACGTgg                                        <  2:753452/150‑1 (MQ=255)

                                                                                                               ggAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGa                                       >  1:359567/1‑150 (MQ=255)

                                                                                                                 aagaaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACTTGGAtt                                     >  2:169928/1‑150 (MQ=255)

                                                                                                                 aagaaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACACTGAACGGCCTTAAAACGTGGAtt                                     >  2:160934/1‑150 (MQ=255)

                                                                                                                  agaaTATATCACCAGGAAATTTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCAGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTa                                    <  1:304292/150‑1 (MQ=255)

                                                                                                                    aaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCTGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGa                                  <  1:1154475/150‑1 (MQ=255)

                                                                                                                      tatatCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGAtt                                >  1:777756/1‑150 (MQ=255)

                                                                                                                        tatCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTa                              <  2:519878/150‑1 (MQ=255)

                                                                                                                         atCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTAc                             <  1:322286/150‑1 (MQ=255)

                                                                                                                             ccAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCATCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAAt                         >  1:1086607/1‑150 (MQ=255)

                                                                                                                              cAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATc                        >  2:289545/1‑150 (MQ=255)

                                                                                                                                ggAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATcac                      <  2:119454/150‑1 (MQ=255)

                                                                                                                                   ggaTTAATGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACata                   >  1:514656/3‑150 (MQ=39)

                                                                                                                                   aaaTTAATGTTGGTGTTATAACCTCACCAACATTAATCCGCTCTGATTATTAATCAGACAAATATCGTTATTCCGTCGAATTTTGATTCTACGTTTTTTCTTACGACACTGAACGGCCTTAAAACGTGGATTAGATTTACAATTCAAata                   >  1:96893/1‑150 (MQ=255)

                                                                                                                                       tAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGc               >  1:1107698/1‑150 (MQ=255)

                                                                                                                                        aaTGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGcc              >  2:1369968/1‑150 (MQ=255)

                                                                                                                                          tGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGATCGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGt            >  2:883089/1‑150 (MQ=255)

                                                                                                                                          tGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCTAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAAAGTGGATTAGATTTACAAATCACATATAGCCGt            >  1:1242744/1‑150 (MQ=255)

                                                                                                                                          tGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGt            >  1:520484/1‑150 (MQ=255)

                                                                                                                                           gTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTc           <  1:923043/150‑1 (MQ=255)

                                                                                                                                            ttGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTcc          <  1:728182/150‑1 (MQ=255)

                                                                                                                                             tGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCt         >  2:501383/1‑150 (MQ=255)

                                                                                                                                                tgtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCTAATTTTGAATCAAAGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCtttt      <  1:237559/150‑1 (MQ=255)

                                                                                                                                                 tttATAAACACTCCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTTAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc     <  1:19551/149‑1 (MQ=255)

                                                                                                                                                 gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAAGGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCtttta     >  1:757719/1‑149 (MQ=255)

                                                                                                                                                 gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc     <  2:1572658/150‑1 (MQ=255)

                                                                                                                                                 gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc     <  2:664907/150‑1 (MQ=255)

                                                                                                                                                 gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc     >  1:520734/1‑150 (MQ=255)

                                                                                                                                                   tataAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTAATTTTCGc   >  1:99880/1‑150 (MQ=255)

                                                                                                                                                    ataAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGc   <  2:1449565/149‑1 (MQ=255)

                                                                                                                                                    ataAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCt  <  1:1456222/150‑1 (MQ=255)

GTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCT  >  NC_000913/312156‑312453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`