BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	312,306	A→T	10.2%	I65I (ATA→ATT) ‡	ykgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	312,306	0	A	T	10.2%	363.5 / 26.1	137	I65I (ATA→ATT) ‡	ykgL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (63/60); new base T (6/8); total (69/68)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

Only 100 of 150 total aligned reads displayed.

TCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCTTC  >  NC_000913/312157‑312455

 cTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATaa                                                                                                                                                      <  2:622075/150‑1 (MQ=255)

 cTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAAGGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCACAGGAAAATTAATGTTGGTGTTATaa                                                                                                                                                      >  1:1591778/1‑150 (MQ=255)

     aaGAGGGTTGATGCCAGCATACAGGGGCGAAGCTTGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCATGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacac                                                                                                                                                  <  1:193817/150‑1 (MQ=255)

     aaGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacac                                                                                                                                                  >  2:947340/1‑150 (MQ=255)

      agagGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCACTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTTTTATAAaccca                                                                                                                                                 >  2:833757/1‑150 (MQ=255)

       gagGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacacac                                                                                                                                                >  1:551682/1‑150 (MQ=255)

          ggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCaa                                                                                                                                             >  2:210795/1‑150 (MQ=255)

           gTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAAc                                                                                                                                            >  1:243419/1‑150 (MQ=255)

           gTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAAc                                                                                                                                            <  1:617109/150‑1 (MQ=255)

            ttGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATTCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACa                                                                                                                                           <  2:411938/150‑1 (MQ=255)

             tGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACAt                                                                                                                                          <  1:1479578/150‑1 (MQ=255)

             tGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACAt                                                                                                                                          <  1:833952/150‑1 (MQ=255)

             tGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACAt                                                                                                                                          <  1:990945/150‑1 (MQ=255)

             tGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACAt                                                                                                                                          <  2:127763/150‑1 (MQ=255)

              gATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATTGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACAtt                                                                                                                                         <  2:192786/150‑1 (MQ=255)

              gATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACAtt                                                                                                                                         >  1:651437/1‑150 (MQ=255)

               aTGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTa                                                                                                                                        >  2:1474283/1‑150 (MQ=255)

                   cAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATcc                                                                                                                                    <  1:574802/150‑1 (MQ=255)

                     gCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTGAAATGAACAGGAAGAATATATCACCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGa                                                                                                                                  >  2:734517/1‑150 (MQ=255)

                     gCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGa                                                                                                                                  >  2:728182/1‑150 (MQ=255)

                       aTACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATc                                                                                                                                >  1:591167/1‑150 (MQ=255)

                         aCAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTg                                                                                                                              >  2:1380968/1‑150 (MQ=255)

                            tgggCGAAGCGGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATTTATCCCCAGTAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGatt                                                                                                                           <  2:1054223/149‑1 (MQ=255)

                             gggCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGatta                                                                                                                          >  2:1099660/1‑150 (MQ=255)

                               gCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGATGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGattatt                                                                                                                        >  2:445894/1‑150 (MQ=255)

                               gCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGattatt                                                                                                                        <  2:208769/150‑1 (MQ=255)

                                cGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGAttatta                                                                                                                       >  1:680702/1‑150 (MQ=255)

                                       ggTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGAc                                                                                                                >  1:171126/1‑150 (MQ=255)

                                       ggTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGAc                                                                                                                >  2:860075/1‑150 (MQ=255)

                                        gTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACa                                                                                                               <  2:1335177/150‑1 (MQ=255)

                                         tCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTAATAATCAGatcg                                                                                                              <  2:418591/150‑4 (MQ=255)

                                          caacaaGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACaaa                                                                                                             >  1:730930/1‑150 (MQ=255)

                                          caacaaGTCAACATTAAGATAATGGAATATACGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATACCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACaaa                                                                                                             >  1:636322/1‑150 (MQ=255)

                                            acaaGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAata                                                                                                           >  2:509551/1‑150 (MQ=255)

                                            acaaGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAata                                                                                                           <  2:380104/150‑1 (MQ=255)

                                                gTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGt                                                                                                       <  2:550647/150‑1 (MQ=255)

                                                 tCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGtt                                                                                                      <  1:1624431/150‑1 (MQ=255)

                                                  cAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATTTTGGTGTTATATACACACCAACATTAATCCGATCTTATTATTAATCAGACAAATATCTTTa                                                                                                     >  2:292977/1‑150 (MQ=255)

                                                    aCATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAAt                                                                                                   >  2:919232/1‑150 (MQ=255)

                                                         aaGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTc                                                                                              <  1:947340/150‑1 (MQ=255)

                                                                 ggAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTg                                                                                      >  2:1434103/1‑150 (MQ=255)

                                                                 ggAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTg                                                                                      <  2:1665597/150‑1 (MQ=255)

                                                                  gAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGa                                                                                     >  2:1253848/1‑150 (MQ=255)

                                                                  gAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGa                                                                                     <  1:1241227/150‑1 (MQ=255)

                                                                   aaTATTCGGAGAGTAACGTCTGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCTTGAAAATTAATGTTGGTGTTATAAACACAGCATCATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGaa                                                                                    <  1:9838/150‑1 (MQ=255)

                                                                             gagaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGtttt                                                                          <  2:284832/150‑1 (MQ=255)

                                                                              agaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTTAATCAACGttttt                                                                         <  1:1200206/150‑1 (MQ=255)

                                                                              agaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGttttt                                                                         >  2:1660883/1‑150 (MQ=255)

                                                                               taaaCGTCATGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGtttttt                                                                        <  1:357498/149‑1 (MQ=255)

                                                                                aaaCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTc                                                                       <  1:14214/150‑1 (MQ=255)

                                                                                 aaCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCt                                                                      <  1:1405846/150‑1 (MQ=255)

                                                                                 aaCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCt                                                                      >  1:519878/1‑150 (MQ=255)

                                                                                  aCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCtt                                                                     <  2:174878/150‑1 (MQ=255)

                                                                                   cGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTa                                                                    <  1:930239/150‑1 (MQ=255)

                                                                                      cAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGa                                                                 >  2:455561/1‑150 (MQ=255)

                                                                                       aGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGAc                                                                <  1:903553/150‑1 (MQ=255)

                                                                                       aGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGAc                                                                <  1:114491/150‑1 (MQ=255)

                                                                                       aGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGAc                                                                <  2:518159/150‑1 (MQ=255)

                                                                                        ggCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCTTCGAATTTTTATTCAACGTTTTTTCTTACGAcc                                                               >  2:844979/1‑150 (MQ=255)

                                                                                         gCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGAccc                                                              <  2:243419/150‑1 (MQ=255)

                                                                                                 ccTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACCCCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGc                                                      >  1:1536849/1‑150 (MQ=255)

                                                                                                  cTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGcc                                                     <  1:860075/150‑1 (MQ=255)

                                                                                                    cAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCtt                                                   <  2:1261619/150‑1 (MQ=255)

                                                                                                       aTGAAAAGGAAGAATATATACCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTaaa                                                <  1:35574/150‑1 (MQ=255)

                                                                                                           aCAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGt                                            >  1:167765/1‑150 (MQ=255)

                                                                                                            cAGGAAGAATATATCCCCATGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTg                                           <  1:1544588/150‑1 (MQ=255)

                                                                                                             aGGAAGAATATATACCCATGAAAATTAATGTTGGTTTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTATCTTACGACCCTGAACGGCCTTAAAACGTgg                                          <  2:753452/150‑1 (MQ=255)

                                                                                                              ggAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGa                                         >  1:390716/1‑150 (MQ=255)

                                                                                                               gaagaaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGAt                                        >  2:381365/1‑150 (MQ=255)

                                                                                                                 agaaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTa                                      >  2:753555/1‑150 (MQ=255)

                                                                                                                 agaaTATATCACCAGGAAATTTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCAGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTa                                      <  1:304292/150‑1 (MQ=255)

                                                                                                                   aaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCTGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGa                                    <  1:1154475/150‑1 (MQ=255)

                                                                                                                     tatatCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGAtt                                  >  2:1221252/1‑150 (MQ=255)

                                                                                                                         tCCCCAGGAAAATTAATGTTGGTGTTATAGACACACCAACATTAATACGATCTGATTTTTAATCAGACAAATATCGTTAAGCCGTCGAATTATGAATCATCGTTTTTTCTTACGACCCTGAACGGCGGTATAACGTGGATTAGATTTTCa                              >  1:715407/1‑150 (MQ=255)

                                                                                                                            ccAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCATCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAAt                           >  1:1086607/1‑150 (MQ=255)

                                                                                                                             cAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATc                          >  2:289545/1‑150 (MQ=255)

                                                                                                                               ggAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATcac                        <  2:551682/150‑1 (MQ=255)

                                                                                                                               ggAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATcac                        <  2:119454/150‑1 (MQ=255)

                                                                                                                                  ggaTTAATGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACata                     >  1:514656/3‑150 (MQ=39)

                                                                                                                                  aaaTTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACata                     <  1:1186365/150‑1 (MQ=255)

                                                                                                                                   gaTTAATGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCtt                                                   <  2:662463/118‑1 (MQ=38)

                                                                                                                                   gaTTAATGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCtt                                                   >  1:662463/2‑119 (MQ=38)

                                                                                                                                      tAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGc                 >  1:1107698/1‑150 (MQ=255)

                                                                                                                                         tGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGATCGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGt              >  2:883089/1‑150 (MQ=255)

                                                                                                                                         tGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCTAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAAAGTGGATTAGATTTACAAATCACATATAGCCGt              >  1:1242744/1‑150 (MQ=255)

                                                                                                                                         tGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGt              >  1:837588/1‑150 (MQ=255)

                                                                                                                                           ttGGTGTAATAAACACACCAACATTAATCCGATCAGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTcc            <  1:734517/150‑1 (MQ=255)

                                                                                                                                            tGGTGTTATAAACACACCAAAATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAATGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCt           <  2:1576497/150‑1 (MQ=255)

                                                                                                                                               tgtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCTAATTTTGAATCAAAGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCtttt        <  1:237559/150‑1 (MQ=255)

                                                                                                                                                tttATAAACACTCCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTTAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc       <  1:19551/149‑1 (MQ=255)

                                                                                                                                                gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAAGGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCtttta       >  1:757719/1‑149 (MQ=255)

                                                                                                                                                gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc       <  2:1572658/150‑1 (MQ=255)

                                                                                                                                                gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc       >  1:764150/1‑150 (MQ=255)

                                                                                                                                                gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc       >  1:520734/1‑150 (MQ=255)

                                                                                                                                                  tataAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTAATTTTCGc     >  1:99880/1‑150 (MQ=255)

                                                                                                                                                   ataAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCt    <  2:1494610/150‑1 (MQ=255)

                                                                                                                                                   ataAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCt    <  2:1141799/150‑1 (MQ=255)

                                                                                                                                                   ataAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCt    <  1:1456222/150‑1 (MQ=255)

                                                                                                                                                    taAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCtt   >  1:413226/1‑150 (MQ=255)

                                                                                                                                                     aaaCACACCAACATTAATCCGATCTGATTATTAATCAGACATATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACTGCCTTAAAACGTGGATTAGATTTACAAATAACATATAGCCGTCCTTTTCGCTTc  >  1:241995/1‑150 (MQ=255)

TCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCTTC  >  NC_000913/312157‑312455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`