Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,687,045 A→T 5.4% intergenic (+20/+25) hmp → / ← glnB fused nitric oxide dioxygenase/dihydropteridine reductase 2/regulatory protein P‑II for glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,687,0450AT 5.4% 302.9 / 3.1 104intergenic (+20/+25)hmp/glnBfused nitric oxide dioxygenase/dihydropteridine reductase 2/regulatory protein P‑II for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base A (49/49);  new base T (2/4);  total (51/53)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

CCGACAATGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACA  >  NC_000913/2686897‑2687192
                                                                                                                                                    |                                                                                                                                                   
ccGACAATGCAGTGCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGaaa                                                                                                                                                    <  1:51629/150‑1 (MQ=255)
 cGACAATGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAACACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAAc                                                                                                                                                   >  1:281164/1‑150 (MQ=255)
 cGACAATGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAAc                                                                                                                                                   >  1:569040/1‑150 (MQ=255)
   aCAATGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACAt                                                                                                                                                 >  1:479346/1‑150 (MQ=255)
        gCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGTTTCCATCCGGc                                                                                                                                            <  1:1558223/150‑1 (MQ=255)
        gCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGc                                                                                                                                            <  1:1529059/150‑1 (MQ=255)
        gCAGGTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGc                                                                                                                                            <  1:1098682/150‑1 (MQ=255)
         cAGTTCTATCTCTGCGGCCCGGGTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCa                                                                                                                                           <  2:397092/150‑1 (MQ=255)
          aGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGTTTCCATCCGGCaa                                                                                                                                          <  1:1019105/150‑1 (MQ=255)
           gTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAAc                                                                                                                                         <  1:1017663/150‑1 (MQ=255)
            ttCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAAcc                                                                                                                                        <  2:1017250/150‑1 (MQ=255)
                aTCTCTGCGGCCCGGGTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGAGGTTGCCGGATGTTTCCATCCGGCAAcatcaa                                                                                                                                    <  1:1731246/150‑6 (MQ=255)
                        ggCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGtt                                                                                                                            >  1:359442/1‑150 (MQ=255)
                         gCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGttt                                                                                                                           <  2:201445/150‑1 (MQ=255)
                          cccGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTa                                                                                                                          <  2:329520/150‑1 (MQ=255)
                            cGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTaaa                                                                                                                        <  1:1501139/150‑1 (MQ=255)
                            cGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTaaa                                                                                                                        >  1:530436/1‑150 (MQ=255)
                             ggTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAAt                                                                                                                       <  1:557042/150‑1 (MQ=255)
                              gtgggCTACATGCTGTTTAGCGCGAAACAGTTAGTGGATCTGGGCGTGAAGGAGGAAAACATTCATTACGAATGCGTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGGCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAAtt                                                                                                                      <  2:1663030/147‑1 (MQ=255)
                                  gCTTCATGCAGTTTACCGCGAAACCGTTAGGGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGAGGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCcg                                                                                                                  <  1:248086/150‑1 (MQ=255)
                                  gCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCcg                                                                                                                  <  1:1425739/150‑1 (MQ=255)
                                  gCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCcg                                                                                                                  >  2:307938/1‑150 (MQ=255)
                                    ttCATGCAGTTTACCGCGAAACAGTTATTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACTAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCcgcg                                                                                                                <  2:1261538/150‑1 (MQ=255)
                                     tCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGcgt                                                                                                               >  2:1108693/1‑150 (MQ=255)
                                     tCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGcgt                                                                                                               <  1:1587207/150‑1 (MQ=255)
                                      cATGCAGTTTACCGCGAAACAGTTTGTGGATCTGGGCGTGTAGCAGGAAATCTTTCATTACTAATGCTTTGGCCCGCATAATGTGCTGTATTTTGATGTTGCCGGATGGAAACATCCGGCAACGCTTGACGCGGTTTAAATTGCCGcgtc                                                                                                              <  1:828513/150‑1 (MQ=255)
                                          ccggtaaCCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTcc                                                                                                          <  2:776068/144‑1 (MQ=255)
                                          cAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTcc                                                                                                          <  2:1535429/150‑1 (MQ=255)
                                          cAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTcc                                                                                                          <  2:1732429/150‑1 (MQ=255)
                                          cAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTcc                                                                                                          <  2:433911/150‑1 (MQ=255)
                                           aGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTGTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCct                                                                                                         <  2:834095/150‑1 (MQ=255)
                                               tACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTc                                                                                                     <  2:1567637/150‑1 (MQ=255)
                                               tACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTc                                                                                                     <  1:837837/150‑1 (MQ=255)
                                                aCCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCa                                                                                                    >  1:300125/1‑150 (MQ=255)
                                                  cgcgAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCAcc                                                                                                  >  2:1438244/1‑150 (MQ=255)
                                                   gcgAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCg                                                                                                 <  1:1346027/150‑1 (MQ=255)
                                                     gAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATGACGAATGCTTTGGCCCGCAGAAGGGGCGGTAATTTGATGTTGCCGGATGGAAACATCCGGCTACCATTGACGCGGTTGAAATTGCAGCGTCGTCCTCTTCCCCGGt                                                                                               >  2:310918/1‑150 (MQ=255)
                                                      aaaCAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTa                                                                                              >  2:703734/1‑150 (MQ=255)
                                                         cAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACgg                                                                                           <  2:1448579/150‑1 (MQ=255)
                                                           gTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGAt                                                                                         <  2:1185144/150‑1 (MQ=255)
                                                            ttAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATg                                                                                        >  1:1349757/1‑150 (MQ=255)
                                                            ttAGTGGACCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGTCGGATGGAAACATCCGGCAACCCTTGACGCGGCTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATg                                                                                        <  2:78420/150‑1 (MQ=255)
                                                             tAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTTCCGGATGGAAACATCCGGCAACCCTTGACGCGGTGTAAATTGCCGCGTCGTCATCTTAACCGGTAAGGATGc                                                                                       >  2:819915/1‑150 (MQ=255)
                                                             tAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGc                                                                                       >  2:628846/1‑150 (MQ=255)
                                                             tAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCATTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGGTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGc                                                                                       >  2:1116702/1‑150 (MQ=255)
                                                              aGTGGATCTGGGCGTGCAGCAGGAAAACATTCATTACGAATGCGTTGGCCCGCATAAGGTGCGGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGCTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCg                                                                                      <  2:1244563/150‑1 (MQ=255)
                                                              aGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCg                                                                                      >  1:592014/1‑150 (MQ=255)
                                                               gTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGa                                                                                     >  1:969517/1‑150 (MQ=255)
                                                                tGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGaa                                                                                    <  2:5568/150‑1 (MQ=255)
                                                                tGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGaa                                                                                    <  1:1516755/150‑1 (MQ=255)
                                                                 ggATCTGGGCGTGAAGCAGGAAAACAGTGATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGATACATCCGGCAACCCTTGACGCGGTTTAAAATGCCGCGTCGTCCTCATCAACGTTACGGAGGCGAAt                                                                                   >  2:240024/1‑150 (MQ=255)
                                                                  gATCTGGTCGTGAATCAGGAAAACATTCATTACGAATGCTTTGGCCAGCATAAGGTGCTGTAATTTGATGTTGCCTGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATg                                                                                  <  1:93930/150‑1 (MQ=255)
                                                                     cTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGAcc                                                                               >  1:1630205/1‑150 (MQ=255)
                                                                      tGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGAccc                                                                              >  1:1722084/1‑150 (MQ=255)
                                                                         gCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTg                                                                           >  2:27889/1‑150 (MQ=255)
                                                                         gCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTg                                                                           <  2:479346/150‑1 (MQ=255)
                                                                           gTGAAGCTGGAAAACATTCATTACGAATGCTTTTGCACGCATAAGGGGCTGTAATTTGATGTTGCCGGATGGAAAAATACGGCAACCATTGCCGCGGTTTAAATTGCCTCGACGTCCTCTACACCGGTACGGATGCGAATGACCCGTGcc                                                                         >  1:182199/1‑150 (MQ=255)
                                                                           gTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGcc                                                                         <  1:734492/150‑1 (MQ=255)
                                                                           gTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGAGGTTGCCGGATGGAATCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGcc                                                                         <  1:249322/150‑1 (MQ=255)
                                                                            tGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACTGATGCGAATGACCCGTGCCa                                                                        >  1:1542925/1‑150 (MQ=255)
                                                                              aaGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACg                                                                      >  1:1415224/1‑150 (MQ=255)
                                                                               aGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGt                                                                     >  2:731030/1‑150 (MQ=255)
                                                                                gCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTc                                                                    <  2:178701/150‑1 (MQ=255)
                                                                                gCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGGGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTc                                                                    <  2:584127/150‑1 (MQ=255)
                                                                                   ggAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCaaa                                                                 >  2:1488236/1‑150 (MQ=255)
                                                                                   ggAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCaaa                                                                 >  2:1222287/1‑150 (MQ=255)
                                                                                   ggAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGAACCGTGCCACGTCaaa                                                                 >  2:971616/1‑150 (MQ=255)
                                                                                   ccaaagCATTCGTAATGAATGTTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCCACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCaaa                                                                 >  1:1086968/3‑150 (MQ=255)
                                                                                   ccaaagCATTCGTAATGAATGTTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCaaa                                                                 >  1:1081359/3‑150 (MQ=255)
                                                                                    caaagCATTCGTAATGAATGTTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCCCCGGTACGGATGCGAATGACCCGTGCCACGTCAAAg                                                                >  2:528578/2‑150 (MQ=255)
                                                                                     aaaaCATTCACAACCAATGCTTTGGCCCGCCTCAGGTGCTGTAATTTGATGGTGCCGGAGGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGa                                                               <  2:1045910/150‑1 (MQ=255)
                                                                                         aattcattACGAATGCTTTGGCGCGCTTAAGGTGCTTTATTTTGATGTTGCCGTATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCTAATGACCCGTGCCACGTCAAAGACGaa                                                           <  2:190129/149‑1 (MQ=255)
                                                                                          attcattACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAg                                                          >  2:789959/1‑150 (MQ=255)
                                                                                          attcattACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAg                                                          >  2:361529/1‑150 (MQ=255)
                                                                                                   gAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCg                                                 <  2:1539052/150‑1 (MQ=255)
                                                                                                        cTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCAcc                                            <  2:889619/150‑1 (MQ=255)
                                                                                                        cTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCAcc                                            >  1:523808/1‑150 (MQ=255)
                                                                                                        cTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCAcc                                            <  1:67384/150‑1 (MQ=255)
                                                                                                                cGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGc                                    >  2:1725717/1‑150 (MQ=255)
                                                                                                                   atatGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCgg                                 >  1:1320535/1‑150 (MQ=255)
                                                                                                                    tAAGGTGCTGTATTTAGATGTTGCCGGATGGAAACCTCCGGCAACCCTTGACGCGGTTTATATTGACGCGTCGTGGTGTTCACCGGTACGGATGGGAATGACCCGTGCCACGTCAATGACGATGATTTTACAGTTGCGGATTTTGTCGGt                                >  2:1089503/1‑150 (MQ=255)
                                                                                                                      aGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCTGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGttt                              <  2:281164/150‑1 (MQ=255)
                                                                                                                           cTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGcc                         >  1:1316852/1‑150 (MQ=255)
                                                                                                                           cTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGcc                         >  1:1325388/1‑150 (MQ=255)
                                                                                                                                 tttGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGa                   <  2:1419777/150‑1 (MQ=255)
                                                                                                                                   tGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACACGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGaat                 <  2:928527/150‑1 (MQ=255)
                                                                                                                                    gATGTTGCAGGTTGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCTCGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGaata                <  1:1444876/150‑1 (MQ=255)
                                                                                                                                     aTGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGaataa               <  2:236060/150‑1 (MQ=255)
                                                                                                                                       gTTGCCGGATGTTTCCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATg             >  2:375988/1‑150 (MQ=255)
                                                                                                                                       gTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATg             <  1:27889/150‑1 (MQ=255)
                                                                                                                                        ttGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCTGCGGTACGTATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGTTTTTGCCGGTTTGCGCCGTGCGAATAATgg            <  2:1042281/150‑1 (MQ=255)
                                                                                                                                           ccGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTAt         >  2:627861/1‑150 (MQ=255)
                                                                                                                                            cGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATc        >  1:439567/1‑150 (MQ=255)
                                                                                                                                            cGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATAGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATc        >  2:675791/1‑150 (MQ=255)
                                                                                                                                             ggATGGAAACTTCCGGCAAAACTTGTCGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGAACCGTGCCACGTCAAAGACGAAGTTTTTACCGTGACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCg       <  1:1131119/150‑1 (MQ=255)
                                                                                                                                             ggATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCg       >  2:707766/1‑150 (MQ=255)
                                                                                                                                             ggATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCg       >  2:736152/1‑150 (MQ=255)
                                                                                                                                                 ggAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGTATAATGGTATCGacac   >  2:1019142/1‑150 (MQ=255)
                                                                                                                                                  gAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGAcaca  >  2:1658756/1‑150 (MQ=255)
                                                                                                                                                  gAAACATCAGGCAACCCTTGACGCGGTTTAAATTGAAGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCAACGTCAAAGACGAAGATTTTACCGTCAGCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGAaaca  >  2:102252/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCGACAATGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACA  >  NC_000913/2686897‑2687192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: