Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,714,358 A→G 6.4% intergenic (+128/+81) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,714,3580AG 6.4% 324.0 / 9.5 109intergenic (+128/+81)srmB/yfiEATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (47/55);  new base G (4/3);  total (51/58)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-01

Only 100 of 112 total aligned reads displayed.

ACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTT  >  NC_000913/2714209‑2714503
                                                                                                                                                     |                                                                                                                                                 
aCCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATa                                                                                                                                                   <  2:820625/150‑1 (MQ=255)
aCCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATa                                                                                                                                                   >  1:1022605/1‑150 (MQ=255)
 ccGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCCGGCCTCCGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATaa                                                                                                                                                  >  2:270264/1‑150 (MQ=255)
  cGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAAAATATTGAATTTGCACGATCTTGTAGGCCGAATAAg                                                                                                                                                 >  1:924640/1‑150 (MQ=255)
   gCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAgg                                                                                                                                                >  1:1232944/1‑150 (MQ=255)
    cAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGTCGGATAAGGc                                                                                                                                               >  2:73839/1‑150 (MQ=255)
    cAAAAGACAGAAGAGAAATCTCAATGCCAGGTCTAAGCCTGCTATTAAAAGTGCTGGCAAACGCAAAACTTCCTGATGCGCTACGCTTATCTGTCCTACGTGGGTCATGAAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGc                                                                                                                                               <  2:252282/150‑1 (MQ=25)
     aaaCGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCg                                                                                                                                              >  1:1074331/1‑150 (MQ=255)
      aaCGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGt                                                                                                                                             >  2:826337/1‑150 (MQ=255)
      aaCGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGt                                                                                                                                             >  1:537969/1‑150 (MQ=255)
        cGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTc                                                                                                                                           >  1:954159/1‑150 (MQ=255)
           cAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcg                                                                                                                                        <  1:492764/150‑1 (MQ=255)
           cAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcg                                                                                                                                        <  1:1429399/150‑1 (MQ=255)
             gaagaGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                                                      <  1:730743/150‑1 (MQ=255)
                 agTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTCGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                                                                                                  >  1:616259/1‑150 (MQ=255)
                  gTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAACCGCAAAACTGCCTGCTGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATGCGGCGTGAAcgccttatc                                                                                                                                 <  2:1284917/150‑6 (MQ=25)
                   tAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTCGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                <  2:395926/150‑1 (MQ=25)
                       ctcAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                                                            <  1:961450/150‑1 (MQ=255)
                       ctcAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                                                            <  2:513715/150‑1 (MQ=255)
                       ctcAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                                                            >  1:68463/1‑150 (MQ=255)
                       ctcAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCATACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                                                            >  1:47293/1‑150 (MQ=255)
                        acAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCATGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGcct                                                                                                                           <  1:1065434/149‑3 (MQ=21)
                         cAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATg                                                                                                                          >  1:622837/1‑150 (MQ=255)
                         cAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATg                                                                                                                          >  1:1027014/1‑150 (MQ=255)
                          aaTGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGa                                                                                                                         >  2:1268113/1‑150 (MQ=255)
                                 ggTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAgc                                                                                                                  <  1:984864/150‑1 (MQ=255)
                                     tAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCCGGCCTACGTGGCTCATGCAATAAATTGAATTTGCACGATCTTGTAGGCCGGTTAAGGAGTTAACGCCGCATCCGGCATGAATAAAGCGCAc                                                                                                              >  2:273700/1‑150 (MQ=21)
                                     tAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCAc                                                                                                              >  1:646687/1‑150 (MQ=255)
                                         ccTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGATCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTg                                                                                                          >  1:550816/1‑150 (MQ=255)
                                           tGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACTCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTc                                                                                                        <  2:1524087/150‑1 (MQ=255)
                                           tGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGTCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTc                                                                                                        <  1:1108727/150‑1 (MQ=255)
                                           tGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTc                                                                                                        <  1:546061/150‑1 (MQ=255)
                                           tGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTc                                                                                                        <  2:1517389/150‑1 (MQ=255)
                                           tGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTc                                                                                                        <  2:67618/150‑1 (MQ=255)
                                           tGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTc                                                                                                        <  2:220411/150‑1 (MQ=255)
                                           tGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTc                                                                                                        <  2:338186/150‑1 (MQ=255)
                                             gTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGa                                                                                                      >  1:1367136/1‑150 (MQ=255)
                                                  aaaaGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTGTGTCGACAAGt                                                                                                 >  1:315906/1‑150 (MQ=255)
                                                   aaaGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGtt                                                                                                <  2:68463/150‑1 (MQ=255)
                                                             cAAACGCAAAACTGCCTGCTGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCggg                                                                                      <  2:1386672/150‑1 (MQ=255)
                                                                aCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAGGCGCACTTTGTCGACAAGTTAAATGCCGGGttt                                                                                   >  2:1344313/1‑150 (MQ=255)
                                                                 cGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTa                                                                                  >  1:162222/1‑150 (MQ=255)
                                                                 cGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTa                                                                                  >  1:1536854/1‑150 (MQ=255)
                                                                   cAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAAt                                                                                >  1:193684/1‑150 (MQ=255)
                                                                   cAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAAt                                                                                >  2:1643851/1‑150 (MQ=255)
                                                                         tGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGAc                                                                          <  2:1292346/150‑1 (MQ=255)
                                                                         tGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGAGCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGAc                                                                          >  1:568574/1‑150 (MQ=255)
                                                                         tGCCTGATGCGCTACGCTTATCAGGCCTAAGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGAc                                                                          <  1:521511/150‑1 (MQ=255)
                                                                             tGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGTCGGGATTAAACCCGGCattt                                                                      <  1:1658336/150‑2 (MQ=255)
                                                                                 gcgcTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTAtttt                                                                  <  1:675457/150‑1 (MQ=255)
                                                                                  cgcTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTAttttt                                                                 <  1:705640/150‑1 (MQ=255)
                                                                                     tACGATTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATc                                                              <  2:117187/150‑1 (MQ=255)
                                                                                      aCGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATcc                                                             <  1:1268113/150‑1 (MQ=255)
                                                                                       cGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCa                                                            >  1:1692703/1‑150 (MQ=255)
                                                                                         cTTATCAGGCCTACGTGGCTCGTGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGc                                                          <  1:408843/150‑1 (MQ=255)
                                                                                         cTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGc                                                          <  2:616259/150‑1 (MQ=255)
                                                                                            aTCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCcac                                                       <  2:1191159/150‑1 (MQ=255)
                                                                                            aTCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCAAGCcac                                                       >  1:1622747/1‑150 (MQ=255)
                                                                                              cAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACaa                                                     >  2:73676/1‑150 (MQ=255)
                                                                                                ggCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACaaaa                                                   >  1:1407835/1‑150 (MQ=255)
                                                                                                ggCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACaaaa                                                   <  2:1706412/150‑1 (MQ=255)
                                                                                                 gCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAAc                                                  <  1:921375/150‑1 (MQ=255)
                                                                                                 gCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAAc                                                  <  1:1701642/150‑1 (MQ=255)
                                                                                                    tACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAActct                                               <  2:954159/150‑1 (MQ=255)
                                                                                                      ggTGGCTCCTGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGTATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAActctct                                             <  2:301633/149‑1 (MQ=255)
                                                                                                      cGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCAGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCGGACATTATTTTTATCCAGCCACATAActctct                                             >  1:501690/1‑150 (MQ=255)
                                                                                                           cTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAac                                        <  2:1719929/150‑1 (MQ=255)
                                                                                                             cATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGTTAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAacac                                      <  2:1102406/150‑1 (MQ=255)
                                                                                                             cATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCACACTTTGTAGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAacac                                      <  2:1229559/150‑1 (MQ=255)
                                                                                                               tGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAacacac                                    >  2:1161122/1‑150 (MQ=255)
                                                                                                                gCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACt                                   >  1:519520/1‑150 (MQ=255)
                                                                                                                    tatatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAAt                               <  1:49833/150‑1 (MQ=255)
                                                                                                                    tatatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGAATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAAt                               <  1:1096267/150‑1 (MQ=255)
                                                                                                                     atatTGAATTTGCACGATCTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATa                              <  1:73839/150‑1 (MQ=255)
                                                                                                                     atatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATa                              >  2:488101/1‑150 (MQ=255)
                                                                                                                        ttGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATaaaa                           <  1:1056356/150‑1 (MQ=255)
                                                                                                                         tGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAg                          >  1:1713125/1‑150 (MQ=255)
                                                                                                                         tGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTAAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAg                          >  1:1642990/1‑150 (MQ=255)
                                                                                                                          gAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCTGCCACAAAACTCTCTTCAACACACTGATTAAAAgt                         >  2:1498483/1‑150 (MQ=255)
                                                                                                                          gAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAgt                         >  2:1498479/1‑150 (MQ=255)
                                                                                                                             tttGCCCGATCTTGTATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTATAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGtgtg                      <  2:136089/150‑1 (MQ=255)
                                                                                                                               tGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGAGATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCa                    >  1:79663/1‑150 (MQ=255)
                                                                                                                                gCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCAt                   >  1:24616/1‑150 (MQ=255)
                                                                                                                                gCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACGGAATAAAAGTGTCCAt                   >  1:1329193/1‑150 (MQ=255)
                                                                                                                                    gATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGcc               <  2:541870/150‑1 (MQ=255)
                                                                                                                                      tCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCgg             <  2:1022605/150‑1 (MQ=255)
                                                                                                                                      tCTTGTAGGCCGGATAAGGCGATCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCgg             <  2:1536854/150‑1 (MQ=255)
                                                                                                                                       cTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCACTTCAACACACTGAATAAAAGTGTGCATCGCCggg            <  2:1074331/150‑1 (MQ=255)
                                                                                                                                        ttGTAGTCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGGCGACAAGTTAAATGCCGGGTGTAATCCAGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGc           <  2:292187/150‑1 (MQ=255)
                                                                                                                                          tcatgCCGGATGCGGCGTGAACGCCTTATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTa         >  1:1152561/5‑150 (MQ=38)
                                                                                                                                           catgCCGGATGCGGCGTGAACGCCTTATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTaa        >  1:1460023/4‑150 (MQ=38)
                                                                                                                                            atgCCGGATGCGGCGTGAACGCCTTATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAAc       >  2:343790/3‑150 (MQ=39)
                                                                                                                                            atgCCGGATGCGGCGTGAACGCCTTATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCACGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAAc       >  1:740077/3‑150 (MQ=38)
                                                                                                                                            aGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGGCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAAc       <  2:1517363/150‑1 (MQ=255)
                                                                                                                                                cGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTTTCGACAAGATAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCt   <  1:286345/150‑1 (MQ=255)
                                                                                                                                                cGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACCAGTGAAATGCCGGGTTTAATCCCGACATTATTTTTATCCCGCCACAAAACTCTCTTCAACACCCTGAATAAAAGTGTGCATCGCCGGGCTAACCGCt   <  2:443207/150‑1 (MQ=255)
                                                                                                                                                 ggATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAAcc      <  2:977519/146‑1 (MQ=255)
                                                                                                                                                 ggATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAAcc      >  1:977519/1‑146 (MQ=255)
                                                                                                                                                 ggATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCtt  >  1:103063/1‑150 (MQ=255)
                                                                                                                                                 ggATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCtt  >  1:240644/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
ACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTT  >  NC_000913/2714209‑2714503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: