Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,716,030 T→G 5.6% intergenic (+20/+36) eamB → / ← grcA cysteine and O‑acetylserine exporter/autonomous glycyl radical cofactor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,716,0300TG 5.6% 308.6 / 6.1 112intergenic (+20/+36)eamB/grcAcysteine and O‑acetylserine exporter/autonomous glycyl radical cofactor
Reads supporting (aligned to +/- strand):  ref base T (54/51);  new base G (4/3);  total (58/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

Only 100 of 113 total aligned reads displayed.

TTTGGCAATGTGTGCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCGGATATTTTTC  >  NC_000913/2715885‑2716179
                                                                                                                                                 |                                                                                                                                                     
tttGGCAATGTGTGCTGGGCGCTGGCGGGGCATCTGGTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGcc                                                                                                                                                   <  1:498835/150‑1 (MQ=255)
  tcgCAATGGCTGCTGGGCGCTTGCGGGGCATCTGATTCAGCGAATGTTTCGCCAGTATGCTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGTTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCct                                                                                                                                                 <  2:71634/148‑1 (MQ=255)
   ggCAATGTGTGCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCctc                                                                                                                                                <  1:259355/150‑1 (MQ=255)
    gCAATGTGTGCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCctct                                                                                                                                               >  1:1442233/1‑150 (MQ=255)
    gCAATGTGTGCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCctct                                                                                                                                               >  2:1419946/1‑150 (MQ=255)
       aTGTGGGCTGGGCGCTGGCGCGGCATCTGTTTCAGCGATTGTTTCGCCAGGAGGGGCGCCAGTTAAATATCGTGCTGGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATAAACGAAAAAAAGCGGAAGAGGGCGACCTCTTcc                                                                                                                                            <  2:1683353/150‑1 (MQ=255)
         gtgtgCTGGGAGCTGGCTGGGCATCTGTTTCAGCGATTGTTGCGCCAGTATGGTCGCCAGTTAAATATCGGGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAATAAGCGTAATAGGTCGCCCTCTTCCGc                                                                                                                                          <  1:208489/150‑1 (MQ=255)
          tgtgCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCt                                                                                                                                         <  2:409411/150‑1 (MQ=255)
            tgCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCACTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTa                                                                                                                                       >  1:124479/1‑150 (MQ=255)
                gggCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTaa                                                                                                                                   <  1:304545/150‑1 (MQ=255)
                 ggCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGGCGACCTCTTCCGCtttttttc                                                                                                                                  <  2:607632/150‑7 (MQ=255)
                   cgcTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGACGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGGCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACtt                                                                                                                                <  2:1103206/150‑1 (MQ=255)
                    gcTGGCGGGGCATCGGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTg                                                                                                                               <  1:1404035/150‑1 (MQ=255)
                       ggCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTGAAATATAGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAACGCGGAAGAGGTCGCCCTCTTCCGAGTAGTAACTTGCTa                                                                                                                            >  2:1077703/1‑150 (MQ=255)
                        gCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTAc                                                                                                                           <  1:1478495/150‑1 (MQ=255)
                        gCGGGGCAGCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTAc                                                                                                                           <  1:50468/150‑1 (MQ=255)
                                  tGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCTCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTa                                                                                                                 >  2:1217408/1‑150 (MQ=255)
                                  tGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTa                                                                                                                 >  1:1198927/1‑150 (MQ=255)
                                   gTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTACCTTGCTACTTAAGCCTTAc                                                                                                                >  2:2787/1‑150 (MQ=255)
                                       cAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCtgtaaggc                                                                                                            >  2:1729428/1‑143 (MQ=255)
                                       cAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGc                                                                                                            >  1:608907/1‑150 (MQ=255)
                                        aGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCt                                                                                                           >  2:1297639/1‑150 (MQ=255)
                                           gATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGGCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGGCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTc                                                                                                        <  2:54432/150‑1 (MQ=255)
                                           gATTCTATCGCCATTATGGTCGCCAGTTAAATAGCGTGCTAGCCCTGTTGCCGGTCTATTTCGCGTTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTc                                                                                                        <  2:288086/150‑1 (MQ=255)
                                            tttGTTTCTCCAGTATGGTCGACAGTTAATTATCCTGCTTGCCCTGTTGCTTGTATATTTCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTGTAAGGCttaag                                                                                                       <  1:124515/149‑4 (MQ=255)
                                            aTTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACTCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCa                                                                                                       <  1:1569949/150‑1 (MQ=255)
                                             ttGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCTGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAg                                                                                                      <  1:1657059/150‑1 (MQ=255)
                                              tGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTGTAAGGCttaagta                                                                                                     <  1:429341/150‑6 (MQ=255)
                                               gTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTa                                                                                                    <  1:588559/150‑1 (MQ=255)
                                               gTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTGTAAGGCttaagtag                                                                                                    <  2:1105472/150‑7 (MQ=255)
                                               gTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTAGCCCTGTTGATGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTa                                                                                                    >  1:113456/1‑150 (MQ=255)
                                                tttCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTaa                                                                                                   >  2:743500/1‑150 (MQ=255)
                                                 ttCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCGGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCAACTTAAGCCTTACAGGCTTTCAGTaaa                                                                                                  >  1:865298/1‑150 (MQ=255)
                                                  tCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAg                                                                                                              >  2:829940/1‑137 (MQ=255)
                                                  tCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAg                                                                                                              <  1:829940/137‑1 (MQ=255)
                                                  tCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAg                                                                                                 <  1:1397175/150‑1 (MQ=255)
                                                   cGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGATGTAGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGACTTACAGGCTTTCAGTAAAgg                                                                                                >  1:174770/1‑150 (MQ=255)
                                                   cGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAgg                                                                                                <  1:1478428/150‑1 (MQ=255)
                                                   cGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACATAAGCCTTACAGGCTTTCAGTAAAgg                                                                                                >  2:1596369/1‑150 (MQ=255)
                                                     ccAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTa                                                                                              >  2:490395/1‑150 (MQ=255)
                                                      cAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTAc                                                                                             <  1:389877/150‑1 (MQ=255)
                                                       aGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTAAAGGCTTTCAGTAAAGGTACg                                                                                            >  2:1023888/1‑150 (MQ=255)
                                                         tATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAg                                                                                          <  2:672369/150‑1 (MQ=255)
                                                             gTCTCCAGTTAAATATCGTGCTTGCCATGTTGATGGTCTATTGCGCGTTACGCATTTTCTATTAACGAAAAATAGCGGAAGTGGTGGACCTCTTCCGGTTAGTATCTTGCTACTTTAGGCTTACAGGCTTTCAGTTAATGTTCGAGCGAt                                                                                      <  2:499821/150‑1 (MQ=255)
                                                             gTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGAt                                                                                      >  2:1561654/1‑150 (MQ=255)
                                                             gTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGAt                                                                                      <  2:1442233/150‑1 (MQ=255)
                                                               cGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATaa                                                                                    >  2:901341/1‑150 (MQ=255)
                                                                   aGTTAAATATCTTGCTTGACCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAACGCGGAAGAGGTCGCCCTCGTCCGCTTAGTAACTTGCTACGTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTc                                                                                <  1:286143/150‑1 (MQ=255)
                                                                   aGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTc                                                                                >  2:118966/1‑150 (MQ=255)
                                                                      tAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTAGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTcgcg                                                                             >  2:771783/1‑150 (MQ=255)
                                                                          tatCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCgcttt                                                                         <  1:1385980/150‑3 (MQ=255)
                                                                            tCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAACAGCGGCAGAGGTCGCCCGCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGctgctg                                                                       <  2:142570/150‑1 (MQ=255)
                                                                            tCGTGCTTGCACTGTTGCTGGTCTATTGCGCTGTACGCATTTTCTATTAGCGAAAAAAAGCGGAAGAGGTCGCCCTCTTACGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGctgctg                                                                       <  2:1096969/150‑1 (MQ=255)
                                                                              gTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCgctgct                                                                        >  2:88957/1‑147 (MQ=255)
                                                                              gTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCgctgct                                                                        <  1:88957/147‑1 (MQ=255)
                                                                                 cTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTATGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCg                                                                  <  1:208380/150‑1 (MQ=255)
                                                                                 cTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCg                                                                  <  1:1161538/150‑1 (MQ=255)
                                                                                    tcccTGTTGCTGGGCTATTGCGCGGGACGCCTTTGCTATTAACGCAAAAACGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAACGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAg                                                               <  1:1077703/149‑1 (MQ=255)
                                                                                     cccTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGATGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTGTCAGTAAAGGTACGATCGATAACGTCGCGCTGCTGTTCCGGAGt                                                              >  1:268121/1‑150 (MQ=255)
                                                                                          ttGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCagag                                                         <  1:1236250/150‑1 (MQ=255)
                                                                                           tGCTGGTCTATTGCGCGGGACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTATCTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGGCagaga                                                        <  2:932061/150‑1 (MQ=255)
                                                                                            gCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCagagag                                                       <  1:118966/150‑1 (MQ=255)
                                                                                             cTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGt                                                      >  2:1276297/1‑150 (MQ=255)
                                                                                               ggTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTa                                                    >  2:809920/1‑150 (MQ=255)
                                                                                                 tCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTaaa                                                  <  2:124479/150‑1 (MQ=255)
                                                                                                    aTTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCg                                               >  2:1501804/1‑150 (MQ=255)
                                                                                                       gcgcGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAAc                                            <  1:1394626/150‑1 (MQ=255)
                                                                                                       gcgcGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAAc                                            >  2:394809/1‑150 (MQ=255)
                                                                                                          cgGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGATCTGc                                         >  2:383309/1‑150 (MQ=255)
                                                                                                           ggTACGCATTTTATATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGCTAACGGCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCa                                        <  1:1138847/150‑1 (MQ=255)
                                                                                                            gTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTATAGCGAACTGCAt                                       >  1:864780/1‑150 (MQ=255)
                                                                                                               cGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAAc                                    >  2:1722634/1‑150 (MQ=255)
                                                                                                               cGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAAc                                    >  2:310378/1‑150 (MQ=255)
                                                                                                                  atggtCTATTAACGAAAAAAAGCGGAAGAGGTGGCCCTCGTCCGCTTAGTAACTTTCTACTTAAGCCTGACAGGCTTTCACTAAAGGTACGAGCGATAGGGTCGCGCTGGTGTTCCGGAGGCAGAGAGTTAAAGCGAACTGCATAAcctg                                 >  2:293346/5‑148 (MQ=255)
                                                                                                                  aTTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGCGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCgg                                 >  1:488226/1‑150 (MQ=255)
                                                                                                                  aTTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCgg                                 <  2:640561/150‑1 (MQ=255)
                                                                                                                    tttCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGAt                               <  2:113456/150‑1 (MQ=255)
                                                                                                                        tATTAACGAACAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACTGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATacac                           <  1:165833/150‑1 (MQ=255)
                                                                                                                         aTTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACg                          >  2:781807/1‑150 (MQ=255)
                                                                                                                           tAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGAAAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGa                        >  2:906571/1‑150 (MQ=255)
                                                                                                                            aaCGAAAAAAAGCGGAAGGGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGAt                       <  1:1291077/150‑1 (MQ=255)
                                                                                                                              cGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAAATGCATAACCGGATACACGGATgg                     >  2:1096669/1‑150 (MQ=255)
                                                                                                                              cGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTACGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATgg                     >  2:149386/1‑150 (MQ=255)
                                                                                                                              cGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGACTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGAtcg                     >  2:149381/1‑148 (MQ=255)
                                                                                                                               gAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGt                    <  1:331477/150‑1 (MQ=255)
                                                                                                                                aaaaaaaGCGGAAGAGGTCGCCCTCTTCCGCTTTGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGCATGGTc                   >  2:1102296/1‑150 (MQ=255)
                                                                                                                                aaaaaaaGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTc                   >  2:1178206/1‑150 (MQ=255)
                                                                                                                                  aaaaaGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAg                 <  1:1177399/150‑1 (MQ=255)
                                                                                                                                     gatcGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTg              >  2:892114/4‑150 (MQ=255)
                                                                                                                                      aGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGc             >  2:660535/1‑150 (MQ=255)
                                                                                                                                      aGCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGc             >  1:632405/1‑150 (MQ=255)
                                                                                                                                       gCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGATCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCg            >  2:45185/1‑150 (MQ=255)
                                                                                                                                       gCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCg            >  1:791794/1‑150 (MQ=255)
                                                                                                                                        cGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCgg           >  1:1610622/1‑150 (MQ=255)
                                                                                                                                        cGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCgg           >  1:1086031/1‑150 (MQ=255)
                                                                                                                                          gAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCGGat         >  2:639687/1‑150 (MQ=255)
                                                                                                                                               ggTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCGGATAtttt    >  1:394118/1‑150 (MQ=255)
                                                                                                                                               ggTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCGGATAtttt    >  1:1684067/1‑150 (MQ=255)
                                                                                                                                               ggTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCATACAGGCTTTCAGTTAAGGTACGAGCGATACCGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGACCTGCATAACCGGATAGACGGATGGTCAGCTGCGGATAtttt    >  2:1489197/1‑150 (MQ=38)
                                                                                                                                                 tCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCGGATATTTTTc  <  2:1198927/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
TTTGGCAATGTGTGCTGGGCGCTGGCGGGGCATCTGTTTCAGCGATTGTTTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAGCGAACTGCATAACCGGATACACGGATGGTCAGCTGCGGATATTTTTC  >  NC_000913/2715885‑2716179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: