Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,736,914 T→A 7.6% intergenic (+31/‑240) bamD → / → raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,736,9140TA 7.6% 322.3 / 10.2 120intergenic (+31/‑240)bamD/raiABamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
Reads supporting (aligned to +/- strand):  ref base T (52/58);  new base A (5/5);  total (57/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 121 total aligned reads displayed.

TACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAA  >  NC_000913/2736766‑2737062
                                                                                                                                                    |                                                                                                                                                     
tACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTg                                                                                                                                                      <  2:642473/150‑1 (MQ=255)
  cccAGGCTACGCGTGATGCGCTGCCTCTGATGGAAAATGCATACCGTAAGATGCAGGTGAATTCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCCAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAg                                                                                                                                                    <  1:888295/150‑1 (MQ=255)
  cccAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAg                                                                                                                                                    <  1:742228/150‑1 (MQ=255)
  cccAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAg                                                                                                                                                    <  2:88946/150‑1 (MQ=255)
   ccAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGc                                                                                                                                                   >  2:1213873/1‑150 (MQ=255)
    cAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCt                                                                                                                                                  >  1:908853/1‑150 (MQ=255)
      ggCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGc                                                                                                                                                >  1:729158/1‑150 (MQ=255)
      ggCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGc                                                                                                                                                >  1:1231190/1‑150 (MQ=255)
         tACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGGCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGt                                                                                                                                             <  2:1676405/150‑1 (MQ=255)
         tACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGt                                                                                                                                             <  1:1271437/150‑1 (MQ=255)
           cgcgTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCGGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGttt                                                                                                                                           <  1:87149/150‑1 (MQ=255)
           cgcgTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGttt                                                                                                                                           <  1:1575708/150‑1 (MQ=255)
            gcgTGATGCGCTGCCGCTGATGGAAAATGCATACCGGCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAACTCATCGCCGCAAACAGCAGCAATACATCACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGtttt                                                                                                                                          <  1:1190924/150‑1 (MQ=255)
            gcgTGATGAGCTGCCGCTGATGGAAAATGCATACCTTCAGATGCAGATGAATGAGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATACCAGAAACCTGAAACACAAAACGGCAGCACGAGCGCTGCCGgttt                                                                                                                                          >  1:445833/1‑150 (MQ=255)
               tGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGttttttt                                                                                                                                       <  2:1052769/150‑1 (MQ=255)
                gATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCCGCCCGTGAGCTGCCGtttttttt                                                                                                                                      >  2:289718/1‑149 (MQ=255)
                gATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTa                                                                                                                                      <  2:488533/150‑1 (MQ=255)
                 aTGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTAt                                                                                                                                     >  2:1382918/1‑150 (MQ=255)
                  tGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTAtt                                                                                                                                    >  1:771230/1‑150 (MQ=255)
                      cTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTTAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGt                                                                                                                                <  2:766443/150‑1 (MQ=255)
                      cTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGt                                                                                                                                <  2:552083/150‑1 (MQ=255)
                       tGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTc                                                                                                                               <  2:1337140/150‑1 (MQ=255)
                        gCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCa                                                                                                                              >  2:1178890/1‑150 (MQ=255)
                          cGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCATTCAGt                                                                                                                           >  2:1148668/1‑150 (MQ=255)
                            cTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTCAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTtg                                                                                                                         >  2:882510/1‑150 (MQ=255)
                              gATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTtgtg                                                                                                                       <  1:1595044/150‑1 (MQ=255)
                              gATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTtgtg                                                                                                                       <  1:903900/150‑1 (MQ=255)
                               aTGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACCGCAGCCCTTGAGCTGCCGTTATTTTATTCTGTCAGTTGTGa                                                                                                                      >  2:310956/1‑150 (MQ=255)
                                tGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGaa                                                                                                                     <  2:1480625/150‑1 (MQ=255)
                                   aaaaTGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATGGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACt                                                                                                                  <  2:701625/150‑1 (MQ=255)
                                     attGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCTTCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAATCACAAAACGGCAGCCCTTGAGGTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGa                                                                                                                <  2:1524638/148‑1 (MQ=255)
                                     aaTGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGa                                                                                                                <  1:1021031/150‑1 (MQ=255)
                                        gCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGGTTTTTTATTCTGTCAGTTGTGAAACTGAAGc                                                                                                             >  1:839119/1‑150 (MQ=255)
                                         cATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCg                                                                                                            >  1:1686257/1‑150 (MQ=255)
                                          aTACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGa                                                                                                           >  2:1661066/1‑150 (MQ=255)
                                               gTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAg                                                                                                      >  2:776485/1‑150 (MQ=255)
                                                tCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGt                                                                                                     <  1:1645729/150‑1 (MQ=255)
                                                      gcagatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTAt                                                                                               >  2:949393/1‑150 (MQ=255)
                                                       cagatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATc                                                                                              <  1:890961/150‑1 (MQ=255)
                                                        agatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTTCCGTTTTTTTATTATGTCAGTTGTGAAACTGAAGCGATTTAGTAGCTATCg                                                                                             >  2:1291002/1‑150 (MQ=255)
                                                        agatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGCCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCg                                                                                             <  2:1053282/150‑1 (MQ=255)
                                                         gatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAACCACAAAACGGCAGCCCTTGAGCTGCCGTTTTTGTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGa                                                                                            >  1:1203311/1‑150 (MQ=255)
                                                         gatgAATGCGCAAGCTGAAAAAGTAGAGAATATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGa                                                                                            <  2:764623/150‑1 (MQ=255)
                                                          atgAATGCGCAAGCTGAAAAATTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGACTAAATCGCttcagtt                                                                                           <  1:429192/150‑7 (MQ=255)
                                                          atgAATGCGCAAGCTGAAAAAGTACCTAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGAt                                                                                           <  2:448608/150‑1 (MQ=255)
                                                           tgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGAtc                                                                                          <  2:1518538/150‑1 (MQ=255)
                                                           tgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGAtc                                                                                          <  2:858168/150‑1 (MQ=255)
                                                            gAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGAtct                                                                                         <  1:744164/150‑1 (MQ=255)
                                                              aTGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCtca                                                                                       >  2:1592438/1‑150 (MQ=255)
                                                                   cAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCaaa                                                                                  >  1:837093/1‑150 (MQ=255)
                                                                    aaGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAat                                                                                 <  2:1297973/150‑1 (MQ=255)
                                                                     aGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAata                                                                                >  1:1107560/1‑150 (MQ=255)
                                                                      gCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTATATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAatat                                                                               >  1:1142334/1‑150 (MQ=255)
                                                                       cTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATg                                                                              <  2:1207658/150‑1 (MQ=255)
                                                                         gAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGc                                                                            <  1:1294897/150‑1 (MQ=255)
                                                                         gAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGc                                                                            >  2:861574/1‑150 (MQ=255)
                                                                              aGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCt                                                                       >  1:1396442/1‑150 (MQ=255)
                                                                                  tctaaaaTCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTTAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTga                                                                   <  1:1616284/147‑1 (MQ=255)
                                                                                   cGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTgag                                                                  <  2:624626/150‑1 (MQ=255)
                                                                                   cGAAAAGAATCGCCTCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTgag                                                                  <  2:941385/150‑1 (MQ=255)
                                                                                    gAAAATCATCGCCGCAAACAGAAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTAGAGAAGCCGTTTTTATATTCAGTCATGTCGTAAACTGAAGCGATTAAGTCGCTATAGATCTCCACAAATATGGCTCGCtgttata                                                                 >  2:181332/1‑144 (MQ=255)
                                                                                        atcatcGCCGCAAACAGCAGCAATTCATAACATAAACCTGAAACACAATACGGCAGCCCTTGAGCGGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTGATCAAATATGGCTCGCTTTGTGATAtt                                                             <  1:761011/150‑1 (MQ=255)
                                                                                        atcatcGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATAtt                                                             >  2:935855/1‑150 (MQ=255)
                                                                                         tcatcGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTATGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTc                                                            >  1:1517380/1‑150 (MQ=255)
                                                                                          catcGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTcc                                                           >  2:333290/1‑150 (MQ=255)
                                                                                          catcGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTATGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCAACAAATATGGCTCGCTTTGATATATTca                                                           >  2:102614/1‑149 (MQ=255)
                                                                                          catcGCCGAAAACAGCAGCAATACATAACAGAAACCTGAATCACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTcc                                                           <  1:185952/150‑1 (MQ=255)
                                                                                              gctgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCCGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCaa                                                       >  1:100783/4‑150 (MQ=255)
                                                                                              gccgcTAACAGCAGCAATACATAACAGAGACCTGAAACACAAAACGGCAGCCCTTGGGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATGTAATACAATATTGCTCGCTTTGAGATATTCCTCaa                                                       >  2:287145/1‑150 (MQ=255)
                                                                                              gccgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGATCGCTTTGAGATATTCCTCaa                                                       >  2:287146/1‑150 (MQ=255)
                                                                                              gcagcAAACAGCAGCAATACATAACAGACACCTGAAACACAAAACGGCAGCCCGTGAGCTGCCGTGTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCaa                                                       >  1:100788/4‑150 (MQ=255)
                                                                                               ccgcAAACAGCTGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAg                                                      <  1:1382918/150‑1 (MQ=255)
                                                                                                    aaCAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCATTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTaaaa                                                 >  2:1428782/1‑150 (MQ=255)
                                                                                                      cagcagCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTaaaaaa                                               >  2:621559/1‑150 (MQ=255)
                                                                                                         cagcaATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACa                                            <  1:1713552/150‑1 (MQ=255)
                                                                                                          agcaATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACAt                                           >  2:1385128/1‑150 (MQ=255)
                                                                                                           gaaaTACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTTTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACAtc                                          <  2:1003655/148‑1 (MQ=255)
                                                                                                              aTACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATctct                                       <  1:861574/150‑1 (MQ=255)
                                                                                                                aCATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTc                                     >  1:1264562/1‑150 (MQ=255)
                                                                                                                   tAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTg                                  >  1:521044/1‑150 (MQ=255)
                                                                                                                      cAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGa                               >  1:977855/1‑150 (MQ=255)
                                                                                                                            ccTGAAACACAAAACGGCAGCCCTTGAGCTGGCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTtctc                         <  1:1376308/150‑1 (MQ=255)
                                                                                                                             cTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTca                        <  1:1029702/150‑1 (MQ=255)
                                                                                                                             cTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGAATAAAAACATCTCTTCCTGCGATTTCTca                        <  1:289718/150‑1 (MQ=255)
                                                                                                                               taaaCACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTcaca                      <  1:1258453/149‑1 (MQ=255)
                                                                                                                                   cacaAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACaaaaa                  >  2:361964/1‑150 (MQ=255)
                                                                                                                                    acaAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACaaaaaa                 >  2:1110948/1‑150 (MQ=255)
                                                                                                                                     aaaaaCGGCAGCTCAAGGGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAg                >  1:998925/2‑150 (MQ=255)
                                                                                                                                     aaaaaCGGCAGCTCAAGGGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAg                >  1:448906/2‑150 (MQ=255)
                                                                                                                                       aaaCGGCAGCTCAAGGGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTACTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGAt              >  2:1278661/1‑150 (MQ=255)
                                                                                                                                            gCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGtt         <  1:1178890/150‑1 (MQ=255)
                                                                                                                                            gCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGtt         >  1:1030180/1‑150 (MQ=255)
                                                                                                                                            gCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGtt         <  2:947522/150‑1 (MQ=255)
                                                                                                                                             cAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTg        <  1:18961/150‑1 (MQ=255)
                                                                                                                                              aGCCCTTGAGCTGCCGTTTTTTTATTCTGTGAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGa       <  1:377416/150‑1 (MQ=255)
                                                                                                                                              aGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGa       <  1:1661066/150‑1 (MQ=255)
                                                                                                                                              aGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGa       >  2:1353281/1‑150 (MQ=255)
                                                                                                                                               gCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGAc      >  2:514250/1‑150 (MQ=255)
                                                                                                                                                cccTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACa     <  2:729158/150‑1 (MQ=255)
                                                                                                                                                   ttGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACaaaa  <  2:837093/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAA  >  NC_000913/2736766‑2737062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: