Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 314,573 G→C 5.1% pseudogene (217/872 nt) eaeH → pseudogene, attaching and effacing protein homology;factor; Not classified

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913314,5730GC 5.1% 359.9 / 6.8 113pseudogene (217/872 nt)eaeHpseudogene, attaching and effacing protein homology;factor; Not classified
Reads supporting (aligned to +/- strand):  ref base G (56/51);  new base C (2/4);  total (58/55)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

Only 100 of 118 total aligned reads displayed.

CGTGGGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTG  >  NC_000913/314424‑314722
                                                                                                                                                     |                                                                                                                                                     
cgtggGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTcg                                                                                                                                                       >  1:1705852/1‑150 (MQ=255)
 gtggGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCGGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTcgc                                                                                                                                                      <  1:357622/150‑1 (MQ=255)
  tggGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTcgcg                                                                                                                                                     >  2:1265310/1‑150 (MQ=255)
         atatCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACGAAAGTAATGGCGGCACTTGCTAAGCAAGCGGTTCAGCCAAGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTg                                                                                                                                              >  1:290248/1‑150 (MQ=255)
         atatCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTg                                                                                                                                              <  1:398530/150‑1 (MQ=255)
          tatCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGc                                                                                                                                             <  2:600070/150‑1 (MQ=255)
           atCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGTTTGAGCATGGGAAATACAACGGTAACTGCTGATAATAACGAGGAGAAAAAAGTCGCGTCCTTTccc                                                                                                                                            >  1:95455/1‑150 (MQ=255)
             ctctGTTCAGGTTCTTTTTCCACTCGCTGTCACGTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGTTAACTGCTGATAATAACGTGGTGAAAAATGTCCCGGCATTTGCGGc                                                                                                                                          <  1:779121/150‑1 (MQ=255)
             ctctGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGc                                                                                                                                          >  1:1691579/1‑150 (MQ=255)
                tGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCTTGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCaaa                                                                                                                                       >  2:1685611/1‑150 (MQ=255)
                tGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCaaa                                                                                                                                       <  1:1557206/150‑1 (MQ=255)
                 gTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCCCGGTAGAGCATGGGAAATACTACGGTAACTGATGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAAt                                                                                                                                      >  1:888145/1‑150 (MQ=255)
                 gTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAAt                                                                                                                                      <  1:1307370/150‑1 (MQ=255)
                 gTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAAt                                                                                                                                      >  1:1688553/1‑150 (MQ=255)
                    cAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGGAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGcc                                                                                                                                   >  2:1137634/1‑150 (MQ=255)
                      ggTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGTTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCgg                                                                                                                                 >  2:856675/1‑150 (MQ=255)
                      ggTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCgg                                                                                                                                 <  2:36424/150‑1 (MQ=255)
                       gTTCATTTTCCACTCGCTGTCTCCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGATCAGCCACGGTTGTTCATGGGATATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCggg                                                                                                                                <  2:1451983/150‑1 (MQ=255)
                            ttttCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACAtt                                                                                                                           >  2:463649/1‑150 (MQ=255)
                              ttCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACAtttt                                                                                                                         >  1:759966/1‑150 (MQ=255)
                               tCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACAttttt                                                                                                                        >  2:685846/1‑150 (MQ=255)
                               tCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACAttttt                                                                                                                        <  2:1024896/150‑1 (MQ=255)
                                ccACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTa                                                                                                                       >  1:1383580/1‑150 (MQ=255)
                                 cACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGATCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCCCGGCATTTGCGGCAAACGACGCGACAtttttct                                                                                                                      <  2:897874/150‑3 (MQ=38)
                                  aCTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCCTGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGATTGCCGCAAATGCCGGGACATTTTTAAg                                                                                                                     >  2:1155608/1‑150 (MQ=255)
                                  aCTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTTGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAg                                                                                                                     <  2:192334/150‑1 (MQ=255)
                                   cTCGCTGTCACCTTTACCCCAGTAATGGCGGCACTTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCCCGGCATTTGCGGCAAACGACGCGACAtttttctcc                                                                                                                    <  1:1530856/150‑5 (MQ=37)
                                   cTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCCCGGCATTTGCGGCAAACGACGCGACAtttttctcc                                                                                                                    <  2:498371/150‑5 (MQ=38)
                                   cTCGCGCTCACCTTTAACCCAGTAAAGGCGGCACGTGCTCAGCATGCGGATCATCGACGGTTGTGTATGGGTAATCCTCCGGCTACGGCTGATACTAACGTGCAGAAAAAAGTCGCCGCGTTAGCCGCAAATGCCGGGACATTTTTAAGc                                                                                                                    <  2:290471/150‑1 (MQ=255)
                                     cGCTCTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATCTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAg                                                                                                                  <  1:1024809/150‑1 (MQ=255)
                                      gCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGt                                                                                                                 >  1:899062/1‑150 (MQ=255)
                                           cACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGcc                                                                                                            <  2:1666338/150‑1 (MQ=255)
                                                  aCCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGc                                                                                                     >  1:695086/1‑150 (MQ=255)
                                                   ccccAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCg                                                                                                    >  1:1697758/1‑150 (MQ=255)
                                                   ccccAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCg                                                                                                    >  1:1527251/1‑150 (MQ=255)
                                                     ccAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGAt                                                                                                  >  1:1192476/1‑150 (MQ=255)
                                                          aaTGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAACTGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGac                                                                                             >  1:1066559/1‑150 (MQ=255)
                                                          aaTGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGac                                                                                             >  1:402623/1‑150 (MQ=255)
                                                          aaTGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGac                                                                                             <  1:790823/150‑1 (MQ=255)
                                                          aaTGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGac                                                                                             <  1:890971/150‑1 (MQ=255)
                                                             ggcggcAGGTGCGCAGCATGCGGGGCAGCCACGGGTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGGCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGAGGCGACACg                                                                                          <  2:95847/150‑1 (MQ=255)
                                                                  cACGTGCGCAGCAGGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACt                                                                                     <  2:918472/150‑1 (MQ=255)
                                                                     gTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAGTACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTtta                                                                                  >  1:401185/1‑150 (MQ=255)
                                                                       gcgcAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTttatt                                                                                <  1:1717274/150‑1 (MQ=255)
                                                                          cagcaTGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGTTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGTACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTAcc                                                                             <  1:1070132/150‑1 (MQ=255)
                                                                             caTGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGa                                                                          >  2:237647/1‑150 (MQ=255)
                                                                             caTGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGa                                                                          <  1:1238414/150‑1 (MQ=255)
                                                                             caTGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGa                                                                          >  2:169948/1‑150 (MQ=255)
                                                                               tGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAAt                                                                        >  2:1242267/1‑150 (MQ=255)
                                                                                gCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATg                                                                       <  1:622998/150‑1 (MQ=255)
                                                                                   gTTCAGCCTCGTTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCTGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGcc                                                                    <  2:1449610/150‑1 (MQ=255)
                                                                                   gTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGcc                                                                    <  2:1294323/150‑1 (MQ=255)
                                                                                      cAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCcaca                                                                 <  1:793859/150‑1 (MQ=255)
                                                                                      cAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCcaca                                                                 >  2:1222380/1‑150 (MQ=255)
                                                                                       aGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACag                                                                >  2:1005916/1‑150 (MQ=255)
                                                                                        gCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGCTGCGACACGTAACTGTATTACCGGAATGGCCACagc                                                               >  2:967709/1‑150 (MQ=255)
                                                                                        gCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCTACACGTAACTTTATTACCGGAATGGCcacatc                                                               >  2:974825/1‑148 (MQ=255)
                                                                                        gCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACagc                                                               >  1:1466668/1‑150 (MQ=255)
                                                                                        gCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACagc                                                               >  2:721284/1‑150 (MQ=255)
                                                                                        gCCACGGTTGAGCATGGGAAATACTACGGTAACTGCAGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACagc                                                               <  2:927738/150‑1 (MQ=255)
                                                                                          cACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACagcta                                                             >  1:817646/1‑150 (MQ=255)
                                                                                             gcttGAGCATGGGAAATACTACGGTAACTGCAGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACagctaaag                                                          <  2:873196/148‑1 (MQ=255)
                                                                                              gTTGAGCATGGGATATCATACGGTAACTGCTGATAATAAAGTGGAGAAAAATGTCGCGGCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAAGTTTATTACCGGAATGGCCACagctaaagc                                                         <  1:281082/150‑1 (MQ=255)
                                                                                                  aGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAAc                                                     <  2:1571561/150‑1 (MQ=255)
                                                                                                   gCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAAcc                                                    <  2:553416/150‑1 (MQ=255)
                                                                                                      tGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATCCGCCCCGTAACATTATTACCGGAATGGCCACAGCTAAAGCTAACCAgg                                                 >  2:1204157/1‑150 (MQ=255)
                                                                                                       gggAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCGACAGCTAATGCTTACCAGGa                                                >  1:1149635/1‑150 (MQ=255)
                                                                                                       gggAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGa                                                >  1:377723/1‑150 (MQ=255)
                                                                                                         gAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGaaa                                              >  1:1484316/1‑150 (MQ=255)
                                                                                                            aTACTACGGTAACTGCTGATAATCACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGCGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATAc                                           <  2:348101/150‑1 (MQ=255)
                                                                                                            aTACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAATCAGGAAATAc                                           <  1:653100/150‑1 (MQ=255)
                                                                                                            aTACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATAc                                           <  1:685846/150‑1 (MQ=255)
                                                                                                               ctacGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCAAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACAAGGAAATACAgg                                        >  2:729671/1‑150 (MQ=255)
                                                                                                               ctaaGGTAACTGCTGATACAAACGTGGAGAATAATGTCGCGTCGTTTGCCGGAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGACCACAGCTAAAGCTAACCAGGAAATACAgg                                        <  2:720819/150‑1 (MQ=255)
                                                                                                                 ccGGTAACTGCTGATAATACCGGGGAGAAAAATGTCGCGTCGGTTGACGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTCCCTGAATGGCCACAGCTAAAGCTAACCAGGCAATACCGGAg                                      <  2:1603634/149‑1 (MQ=255)
                                                                                                                  cGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGt                                     <  2:785951/150‑1 (MQ=255)
                                                                                                                   ggTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTg                                    <  1:635622/150‑1 (MQ=255)
                                                                                                                             gATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGCTAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGaaa                          >  1:947514/1‑150 (MQ=255)
                                                                                                                             gATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGaaa                          >  1:312843/1‑150 (MQ=255)
                                                                                                                              ataataACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAat                         >  1:1680814/1‑150 (MQ=255)
                                                                                                                              ataataACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAat                         >  2:491545/1‑150 (MQ=255)
                                                                                                                               taataaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAata                        >  1:1641279/1‑150 (MQ=255)
                                                                                                                                aataaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAatat                       <  2:1273248/150‑1 (MQ=255)
                                                                                                                                 ataaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATg                      <  1:393450/150‑1 (MQ=255)
                                                                                                                                  taaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTAGCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATgg                     <  1:1394558/150‑1 (MQ=255)
                                                                                                                                  taaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATgg                     <  1:1274465/150‑1 (MQ=255)
                                                                                                                                  taaCGTGGAGAAAAATGTCGAGTCGTTTGCCGCAAATGCCGTGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACTCGTATCTTTATTTCCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATgg                     <  2:95455/150‑1 (MQ=255)
                                                                                                                                     cGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTAc                  <  2:1383580/150‑1 (MQ=255)
                                                                                                                                       tGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTg                <  2:1527251/150‑1 (MQ=255)
                                                                                                                                        ggAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTgc               <  2:552693/150‑1 (MQ=255)
                                                                                                                                        ggAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACAAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTgc               >  2:967061/1‑150 (MQ=255)
                                                                                                                                        gcttaaaaaTGTCCCGGCATTTGCGGCAAACGACGCGACAGTTTTAAGCAGTCAGCCAGAGAGCGATGCGACACGTAACTTTATTCCCGGAATGGCCACAGCTAAAGATAACCAGGACATACAGGAGTGGCTCGTGTAATATGGTCCTgc               >  1:81046/5‑150 (MQ=25)
                                                                                                                                          agaAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACAAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTgcgc             >  2:1045000/1‑150 (MQ=255)
                                                                                                                                          agaAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCAAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATTGTACTgcgc             >  2:158980/1‑150 (MQ=255)
                                                                                                                                          agaAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTGAAGCAGTCAGCCAGATAGCGATGCGTCACGTAACTTTATTACCGGAATGGCCCCAGCTTAGGCTATCGAGGAAATACAGGAGTGGCTCGGGAAATAGGGTACTgcgc             >  1:318573/1‑150 (MQ=255)
                                                                                                                                              aaaTGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGTCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGt         <  2:888145/150‑1 (MQ=255)
                                                                                                                                               aaTGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATAGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGcgcgcggc        <  1:1513026/150‑3 (MQ=255)
                                                                                                                                                  gTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAACCAGTCAGCCAGATAGCGATGCGACAAGTAACTTTATTACCGGAATGGCCACACCAAAAGCTAACCAGGAAATACAGGAGTCGGTCGGGAAATATGGTAATGCGAGCGACaaa     >  2:124225/1‑150 (MQ=255)
                                                                                                                                                    cgcgTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACt   <  2:1477720/150‑1 (MQ=255)
                                                                                                                                                     gcgTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAAccg  >  1:372720/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGTGGGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTG  >  NC_000913/314424‑314722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: