Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,754,945 A→T 5.7% N17I (AAC→ATC) ‡ smpB → tmRNA‑binding trans‑translation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,754,9450AT 5.7% 417.1 / 8.3 145N17I (AAC→ATC) ‡smpBtmRNA‑binding trans‑translation protein
Reads supporting (aligned to +/- strand):  ref base A (70/66);  new base T (5/4);  total (75/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

CTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGTTTG  >  NC_000913/2754798‑2755094
                                                                                                                                                   |                                                                                                                                                     
cTGGGTAACATCGGGTTCATGCTAAGATAGATCCTTGTCGCCCGCTGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACa                                                                                                                                                     <  2:1166642/150‑1 (MQ=255)
  gggTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGTATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAg                                                                                                                                                   <  1:706461/150‑1 (MQ=255)
  gggTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACaac                                                                                                                                                   >  1:1056209/1‑149 (MQ=255)
      aaCATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGAGATGGGGGGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCCTCGCGCTTAACAAgcgcg                                                                                                                                               >  2:1563104/1‑150 (MQ=255)
       aCATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAgcgcgc                                                                                                                                              >  2:271831/1‑150 (MQ=255)
       aCATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGATCAGCGACCATCGCGCTTAACAAgcgcgc                                                                                                                                              >  2:1618298/1‑150 (MQ=255)
        cATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAAACTGTTTCAGCGACCATCTCGCTTAACAAGCGCTcc                                                                                                                                             >  2:1008042/1‑150 (MQ=255)
          tCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTTATATGGGGTGTTTTCGATTTCAGATAACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTGTTAAgcgcgatgg                                                                                                                                           <  2:86895/150‑5 (MQ=255)
           cGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGt                                                                                                                                          >  2:1117000/1‑150 (MQ=255)
             ggTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCa                                                                                                                                        >  2:437860/1‑150 (MQ=255)
              gTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCAc                                                                                                                                       <  1:1228069/150‑1 (MQ=255)
              gTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCAc                                                                                                                                       <  2:1206864/150‑1 (MQ=255)
                 cATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCTATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTAACGaa                                                                                                                                    >  1:808283/1‑150 (MQ=255)
                  aTGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAAt                                                                                                                                   <  1:681459/150‑1 (MQ=255)
                   tGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATa                                                                                                                                  >  1:1305914/1‑150 (MQ=255)
                     cTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACt                                                                                                                                >  1:358277/1‑150 (MQ=255)
                     cTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACt                                                                                                                                >  2:803581/1‑150 (MQ=255)
                     cTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACt                                                                                                                                <  1:578417/150‑1 (MQ=255)
                      tAAGATAGAGCCTTGTCCCCCGCTGGATTGATATGGGGTGTTTTCGATTTCAGATTACCTATGATTCACGACGCTTATGACGAATAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACtt                                                                                                                               <  2:757985/150‑1 (MQ=255)
                        aGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTa                                                                                                                             >  1:769839/1‑150 (MQ=255)
                          aTAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATc                                                                                                                           >  1:1200737/1‑150 (MQ=255)
                           tAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCg                                                                                                                          >  2:719120/1‑150 (MQ=255)
                           tAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCg                                                                                                                          <  2:1113696/150‑1 (MQ=255)
                              agCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCgaag                                                                                                                       <  2:579822/150‑1 (MQ=255)
                               gCCTTGGCCCCCGCAGGATTGATATGTGGTGTTTTCGATTTCAGATTACCGATGATTCACGACTCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCgaaga                                                                                                                      <  2:147659/150‑1 (MQ=255)
                                      cccccGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTc                                                                                                               <  2:750586/150‑1 (MQ=255)
                                       ccccGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCg                                                                                                              <  2:964939/150‑1 (MQ=255)
                                       ccccGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAAGACTTTATCGAAGAAGAGTTCg                                                                                                              >  2:550807/1‑150 (MQ=255)
                                                        ggggTGTTTTCGATTTCAGATTACCGATGATTCACGAAGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCTTCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTg                                                                                             <  1:1127515/150‑1 (MQ=255)
                                                         gggTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGc                                                                                            >  2:890451/1‑150 (MQ=255)
                                                          ggTGTTTTCGATTTCAGATTACCTATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCa                                                                                           <  2:269761/150‑1 (MQ=255)
                                                          ggTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCa                                                                                           >  1:1247225/1‑150 (MQ=255)
                                                          ggTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCa                                                                                           >  1:1587993/1‑150 (MQ=255)
                                                            tgtTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAg                                                                                         <  1:803581/150‑1 (MQ=255)
                                                             gtTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAgg                                                                                        <  1:1117000/150‑1 (MQ=255)
                                                                 tagATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTgg                                                                                    <  1:104457/148‑1 (MQ=255)
                                                                  cGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTAATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGAAAGGCTggg                                                                                   >  2:1537765/1‑150 (MQ=255)
                                                                  cGATTTCAGAATACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCATAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTggg                                                                                   <  2:939943/150‑1 (MQ=255)
                                                                   gATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGa                                                                                  <  1:1451864/150‑1 (MQ=255)
                                                                   gATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGa                                                                                  <  1:360564/150‑1 (MQ=255)
                                                                   gATTTCAGATTACCGAGGATTCACGACGCTTATGACGAAGAAAACAGCACATAAACCTGGTGCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCNAAGAAGAGTTCGAAGCGGGNCTTGCCCTGCAAGGCTgngn                                                                                  <  2:1401336/150‑2 (MQ=255)
                                                                    aTTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGaa                                                                                 >  2:419429/1‑150 (MQ=255)
                                                                     tttCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAg                                                                                <  1:414250/150‑1 (MQ=255)
                                                                     attCAGATTACCGATGATTCACGACGCGTATGACGAAGAAAAAAGCACATACACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGTGACTTGCCCTGCAAGGCTGGGAAg                                                                                <  2:1158861/149‑1 (MQ=255)
                                                                       tCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTAATCGAAGAAGAGTACGAAGCGGGACTTGCCCTGCAAGACTGGGAAGtt                                                                              >  1:1456183/1‑150 (MQ=255)
                                                                            ttACCTATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATc                                                                         <  1:499106/150‑1 (MQ=255)
                                                                            ttACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTTAATc                                                                         <  2:449856/150‑1 (MQ=255)
                                                                              aCCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAATCCTGGTTCAGCGACCATCGCGCTTAACAAGCGCTCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCCGGACTTGCCCTGCAAGGCTGGGAAGATAAATccc                                                                       >  1:1377514/1‑150 (MQ=255)
                                                                              aCCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATccc                                                                       >  1:673964/1‑150 (MQ=255)
                                                                                cGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTg                                                                     >  2:1216579/1‑150 (MQ=255)
                                                                                      ttCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGTGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCa                                                               >  2:1691282/1‑150 (MQ=255)
                                                                                       tCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAg                                                              <  1:667877/150‑1 (MQ=255)
                                                                                        cACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAgg                                                             >  2:1573736/1‑150 (MQ=255)
                                                                                        cACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAgg                                                             >  2:1566743/1‑150 (MQ=255)
                                                                                         acgacgCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGa                                                            >  1:1497413/1‑150 (MQ=255)
                                                                                          cgacgCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCCTCGCGATTAACAAGCGCGCCCGTCACGAATACTATATCGAAGAAGAGTTCGCAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGaa                                                           >  1:34842/1‑150 (MQ=255)
                                                                                               cTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGcc                                                      <  1:468784/150‑1 (MQ=255)
                                                                                               cTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGcc                                                      <  1:271831/150‑1 (MQ=255)
                                                                                               cTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGcc                                                      >  2:1417204/1‑150 (MQ=255)
                                                                                                ttATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCa                                                     <  1:1383738/150‑1 (MQ=255)
                                                                                                  aTGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAat                                                   >  2:1677183/1‑150 (MQ=255)
                                                                                                   tcaCGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCTCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGTCGTTAAATCCCTGCGCGCAGGAAAAGACAata                                                  >  1:256377/3‑150 (MQ=255)
                                                                                                   tGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCGGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGACCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGATAAGCCAata                                                  >  1:252737/1‑150 (MQ=255)
                                                                                                    gACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAACCGGGACTTGCCCTGCAAAGCTGCGAACTTAAATCCCTGCGCGCAGGAAACGCCAatat                                                 >  2:1122218/1‑150 (MQ=255)
                                                                                                     aCGAAGAAAAAAGCACATAAACCTGGTTCATCGACCAGCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATc                                                <  1:701977/150‑1 (MQ=255)
                                                                                                           aaaaaaGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGAc                                          <  2:1144861/150‑1 (MQ=255)
                                                                                                              aaaGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGc                                       <  1:955509/150‑1 (MQ=255)
                                                                                                                   acctcaaCCTGGTGCAGCGACCATCGCGCTTAACACGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGt                                  <  1:1117455/145‑1 (MQ=255)
                                                                                                                    caTAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATAGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTc                                 >  2:90045/1‑150 (MQ=255)
                                                                                                                        aaCCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTc                             <  1:550807/150‑1 (MQ=255)
                                                                                                                         aCCTGGTTCAGCGACCTTCGCGCTTAACAAGCGCGCCCGGCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCt                            <  1:291013/150‑1 (MQ=255)
                                                                                                                         aCCTGGTGCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGACTACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCt                            <  2:1497436/150‑1 (MQ=255)
                                                                                                                          ccTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTg                           >  1:72408/1‑150 (MQ=255)
                                                                                                                          ccTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGACACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCAGCACGCCTGGGAAGTTAAATCCCTGCGAGAACGAAAAGCCAAGATCAGCGACACCGACGTCctgctg                           >  2:394512/1‑150 (MQ=255)
                                                                                                                             ggTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGctt                        >  2:225363/1‑148 (MQ=255)
                                                                                                                             ggTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGt                        >  2:17755/1‑150 (MQ=255)
                                                                                                                             ggTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAACGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGt                        >  2:1139512/1‑150 (MQ=255)
                                                                                                                               ttCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGATGTTAAATCCCTGCGCGCAGGATAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGa                      >  2:1460083/1‑150 (MQ=255)
                                                                                                                               ttCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGa                      <  1:437860/150‑1 (MQ=255)
                                                                                                                                  aGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACgg                   <  1:1372263/150‑1 (MQ=255)
                                                                                                                                  aGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACgg                   >  1:483311/1‑150 (MQ=255)
                                                                                                                                  aGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACgg                   >  2:592858/1‑150 (MQ=255)
                                                                                                                                   gCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGga                  >  2:49464/1‑150 (MQ=255)
                                                                                                                                       cgggcgcgCTTGTTAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAgg              >  2:1272921/5‑150 (MQ=255)
                                                                                                                                       ccATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAgg              <  2:1610707/150‑1 (MQ=255)
                                                                                                                                       ccATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAgg              <  1:217944/150‑1 (MQ=255)
                                                                                                                                       ccATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAgg              <  2:1587993/150‑1 (MQ=255)
                                                                                                                                        cATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGc             >  2:1686795/1‑150 (MQ=255)
                                                                                                                                        cATCGCGCTAAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCCTCTGCGTGACGGAGAGGc             <  2:350513/150‑1 (MQ=255)
                                                                                                                                         ggcgcgCTTGTTAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCa            >  1:1182103/3‑150 (MQ=255)
                                                                                                                                         aTCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCa            >  2:227622/1‑150 (MQ=255)
                                                                                                                                          tCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCAt           >  1:1372121/1‑150 (MQ=255)
                                                                                                                                           cgcgCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCAtt          >  2:634329/1‑150 (MQ=255)
                                                                                                                                            gcgcTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCAttt         >  1:474110/1‑150 (MQ=255)
                                                                                                                                             cgcTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTc        >  1:771697/1‑150 (MQ=255)
                                                                                                                                              gcTTATCAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCGTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTCt       <  2:34842/150‑1 (MQ=255)
                                                                                                                                                 tAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGtt    <  1:1548403/150‑1 (MQ=255)
                                                                                                                                                  aaCAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGTCTTGCCCTGCAAGGCTGTGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCCGCGACCGCTACGTCCTTCTGCGTGCCGGAGAGGCCTTTCTGttt   >  2:1302931/1‑150 (MQ=255)
                                                                                                                                                  aaCAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGttt   <  1:939788/150‑1 (MQ=255)
                                                                                                                                                   aCAAGCGCGCACGTCACTAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGTTTg  <  1:1437184/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGATTACCGATGATTCACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGTTTG  >  NC_000913/2754798‑2755094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: