Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,757,420 C→A 7.6% intergenic (+20/+224) intA → / ← yfjH CP4‑57 prophage; integrase/CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,757,4200CA 7.6% 349.6 / 10.8 127intergenic (+20/+224)intA/yfjHCP4‑57 prophage; integrase/CP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (56/60);  new base A (6/5);  total (62/65)
Fisher's exact test for biased strand distribution p-value = 7.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

CGCTTATAACCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTGCTCCTTTTG  >  NC_000913/2757271‑2757567
                                                                                                                                                     |                                                                                                                                                   
cgcTTATAACCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGATCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGctc                                                                                                                                                     >  2:1178059/1‑150 (MQ=255)
cgcTTATAACCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGctc                                                                                                                                                     <  1:993346/150‑1 (MQ=255)
  cTTATAACCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGctctc                                                                                                                                                   >  1:1121043/1‑150 (MQ=255)
      taACCGCAGTGATTATCTTGAGCAACGTCGTCCGTTGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTggg                                                                                                                                               <  1:1403843/150‑1 (MQ=255)
      taACCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTggg                                                                                                                                               >  1:33851/1‑150 (MQ=255)
       aaCCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGc                                                                                                                                              >  2:1132462/1‑150 (MQ=255)
       aaCCGCAGAGATTATCTTGAGCAACGTCGTCCGATGATGCAATCGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTAGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTcggg                                                                                                                                              >  2:255922/1‑149 (MQ=255)
        aCCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCt                                                                                                                                             >  1:1049939/1‑150 (MQ=255)
        aCCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCt                                                                                                                                             <  1:1217473/150‑1 (MQ=255)
         ccGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTg                                                                                                                                            >  1:777281/1‑150 (MQ=255)
         ccGAAGTGATTATCTTGAGCAACGTCGTCCGTTGATGCAATGTTGTGCTTATCTCTTAAAATCATCAGATTGTGGTAGCATCGTTTTAACTCATTTTAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTg                                                                                                                                            <  2:1215924/150‑1 (MQ=255)
           gCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGat                                                                                                                                          >  1:553263/1‑150 (MQ=255)
           gCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGAGAGCTGGGCTGat                                                                                                                                          <  1:243656/150‑1 (MQ=255)
            cAGTGATTATCTTGAGCAAAGTCGTCCGATGATGATATGGTTGGCTGATCTCGTAAAATCAGCAGATAGTGGTAGCATCGTTTTAACTCAATTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGAGACCTGGGCTGata                                                                                                                                         <  1:259163/150‑1 (MQ=255)
              gTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGTCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGAGAGCTGGGCTGataaa                                                                                                                                       <  1:1237698/150‑3 (MQ=255)
               tGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGTAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGAGAGCTGGGCTGataaat                                                                                                                                      <  1:278616/150‑4 (MQ=255)
                    aTCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTaaa                                                                                                                                 >  2:1423486/1‑150 (MQ=255)
                       ttGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCTTAAAAGCAGCAGCTAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTAGTCGTGTCGGAAAAAATATTTATAAGCCCACCTATCTTGGCTGATATTTCTAAAAGa                                                                                                                              >  2:1647239/1‑150 (MQ=255)
                       ttGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCCGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGa                                                                                                                              >  2:852821/1‑150 (MQ=255)
                        tGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGAt                                                                                                                             >  2:627952/1‑150 (MQ=255)
                        tGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGAt                                                                                                                             >  2:1388878/1‑150 (MQ=255)
                           gCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGc                                                                                                                          <  2:1675257/150‑1 (MQ=255)
                               cgtcgtCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTa                                                                                                                      <  2:807880/150‑1 (MQ=255)
                                      cGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCt                                                                                                               >  1:1653946/1‑150 (MQ=255)
                                         tgatgCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTAcg                                                                                                            <  2:154522/150‑1 (MQ=255)
                                         tgatgCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTAcg                                                                                                            <  1:385096/150‑1 (MQ=255)
                                           atgCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTAcgcg                                                                                                          <  2:1376067/150‑1 (MQ=255)
                                            tgCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCgg                                                                                                         >  1:1244001/1‑150 (MQ=255)
                                               aaTGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAAt                                                                                                      >  1:774759/1‑150 (MQ=255)
                                               aaTGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTAAGCGGAAt                                                                                                      >  1:183783/1‑150 (MQ=255)
                                                 tggtggGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAg                                                                                                    <  1:806816/150‑1 (MQ=255)
                                                 tggtggGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAg                                                                                                    <  2:1522581/150‑1 (MQ=255)
                                                    tggGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCTCTAAATAGCTACGCGGAATAGTGc                                                                                                 <  2:637440/150‑1 (MQ=255)
                                                    tggGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCTCTAAATAGCTACGCGGAATAGTGc                                                                                                 >  1:637440/1‑150 (MQ=255)
                                                    tggGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGc                                                                                                 <  2:356104/150‑1 (MQ=255)
                                                    tggGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGc                                                                                                 >  1:478163/1‑150 (MQ=255)
                                                      ggCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGAAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTATAAAAGATAGAACTAAATAGCTACGCGGAATAGTGctc                                                                                               >  1:1302396/1‑150 (MQ=255)
                                                        cTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTATAAAAGATCGCAATAAATAGCTACGCGGAATAGTGCtata                                                                                             >  1:167026/1‑150 (MQ=255)
                                                        cTGATCTCGAAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTTAAAGATCGCACTAAATAGATAAGCGGAATAGTGCTCTa                                                                                             >  1:105176/1‑150 (MQ=255)
                                                           aTCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGATCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACtt                                                                                          <  2:234813/150‑1 (MQ=255)
                                                           aTCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGAAAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACtt                                                                                          <  1:257771/150‑1 (MQ=255)
                                                              tcGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCg                                                                                       >  1:1230592/1‑150 (MQ=255)
                                                               cGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGCTAAAAAATATATCAGCCCAGCTCTCTCGGCTGATATTTCTAAACGATCGCACTAAAAAGCTACGCGGAATAGTGCTCTACTTTCGt                                                                                      <  2:726726/150‑1 (MQ=255)
                                                                  aaaaGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTa                                                                                   >  2:1569301/1‑150 (MQ=255)
                                                                  aaaaGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTATCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTa                                                                                   >  2:1449963/1‑150 (MQ=255)
                                                                   aaaGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAg                                                                                  <  1:490102/150‑1 (MQ=255)
                                                                   aaaGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAg                                                                                  >  2:1587397/1‑150 (MQ=255)
                                                                   aaaGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAg                                                                                  <  2:1708351/150‑1 (MQ=255)
                                                                     agcagcAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGcc                                                                                <  2:1121043/150‑1 (MQ=255)
                                                                        agcagATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCtt                                                                             <  2:777281/150‑1 (MQ=255)
                                                                          cagATAGTTGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTc                                                                           <  2:1409429/150‑1 (MQ=255)
                                                                          cagATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTc                                                                           <  2:1231966/150‑1 (MQ=255)
                                                                           agATAGTGGTAGCATCGTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCag                                                                         <  2:490602/150‑1 (MQ=255)
                                                                            gATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCag                                                                         >  1:974436/1‑150 (MQ=255)
                                                                            gATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGGCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCag                                                                         <  2:39163/150‑1 (MQ=255)
                                                                             aTAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCaga                                                                        <  1:954676/150‑1 (MQ=255)
                                                                              tAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCagag                                                                       <  1:1402359/150‑1 (MQ=255)
                                                                                   gTAGCATAGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAg                                                                  <  2:791159/150‑1 (MQ=255)
                                                                                       cATCGTTATAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGATCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACAt                                                              >  2:619977/1‑150 (MQ=255)
                                                                                         tCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTa                                                            <  2:847199/150‑1 (MQ=255)
                                                                                          cGTTTTAACTCATTTGATCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTCGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGCAATACTGCTCTACTTTCGATTAGCCCTTTCACAGGTAAGACATTAg                                                           >  2:1560035/1‑150 (MQ=255)
                                                                                          cGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAg                                                           <  1:1326046/150‑1 (MQ=255)
                                                                                            ttttAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAggg                                                         <  1:1423486/150‑1 (MQ=255)
                                                                                               tAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCaa                                                      >  2:574646/1‑150 (MQ=255)
                                                                                               tAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCaa                                                      >  1:26418/1‑150 (MQ=255)
                                                                                                  cTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAAttt                                                   <  1:1414417/150‑1 (MQ=255)
                                                                                                   tCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTc                                                  <  1:1476503/150‑1 (MQ=255)
                                                                                                    cATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCa                                                 <  2:1049939/150‑1 (MQ=255)
                                                                                                     aTTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCAc                                                <  1:852821/150‑1 (MQ=255)
                                                                                                     aTTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCAc                                                <  1:1647239/150‑1 (MQ=255)
                                                                                                      tttGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGTGATGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACt                                               <  1:807834/150‑1 (MQ=255)
                                                                                                       ttGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTg                                              <  1:1186276/150‑1 (MQ=255)
                                                                                                          aGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTAt                                           >  1:631156/1‑150 (MQ=255)
                                                                                                             aaaaTTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGAc                                        <  1:1561344/150‑1 (MQ=255)
                                                                                                                     tCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGt                                <  1:1345976/150‑1 (MQ=255)
                                                                                                                       ttGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCa                              <  1:194930/150‑1 (MQ=255)
                                                                                                                        tGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCAt                             <  2:868551/150‑1 (MQ=255)
                                                                                                                           cGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCAtaca                          <  1:619977/150‑4 (MQ=255)
                                                                                                                            ggATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCAtacag                         <  1:466966/150‑5 (MQ=255)
                                                                                                                            ggATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCAtacag                         <  2:474164/150‑5 (MQ=255)
                                                                                                                            ggATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAg                         >  1:746844/1‑150 (MQ=255)
                                                                                                                             gATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCAtacagt                        <  2:1230592/150‑6 (MQ=255)
                                                                                                                              aTAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTc                       <  2:1653946/150‑1 (MQ=255)
                                                                                                                                aaaaTATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTACGACATTACGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCtt                     >  2:73187/1‑149 (MQ=255)
                                                                                                                                aaaaTATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCtg                     >  1:1531099/1‑150 (MQ=255)
                                                                                                                                 aaaTATTTATCAGCCCAGCGCTCTGGGCTGATATTTCTAAATGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCtgt                    >  1:524881/1‑150 (MQ=255)
                                                                                                                                   atatTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTgg                  >  1:1177925/1‑150 (MQ=255)
                                                                                                                                   agaaatATCAGCCCAGAGAGCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCCGTCATCGAGTCTGTgg                  >  2:1569271/6‑150 (MQ=255)
                                                                                                                                    tatTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTggg                 >  1:1042514/1‑150 (MQ=255)
                                                                                                                                     atTTATCAGCCCAGCTCTCTGTGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTgggg                <  2:371384/150‑1 (MQ=255)
                                                                                                                                     atTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTgggg                >  2:435367/1‑150 (MQ=255)
                                                                                                                                        tATCAGCCCAGAGAGCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCTATTTCACTGTATGACTGCGCAGTCATCGAGTGTGTGGGgtgt             >  1:173193/1‑150 (MQ=255)
                                                                                                                                         aTCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGtt            >  1:571316/1‑150 (MQ=255)
                                                                                                                                         aTCAGCCCAGAGAGCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCGGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGtt            >  2:711888/1‑150 (MQ=255)
                                                                                                                                          tCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTCCGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTg           <  2:605548/150‑1 (MQ=255)
                                                                                                                                          tCAGCCCAGAGAGCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCGACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTg           >  1:832679/1‑150 (MQ=255)
                                                                                                                                           cAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCATTCAGAGGTAAGACATTAGGGCAATTACACTGTATGAATGCGCAGTCATCGAGTCTGTGGGGTCTTGc          >  2:597736/1‑150 (MQ=255)
                                                                                                                                             gCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGGCTGTGGGGTGTTGCTc        >  2:1400780/1‑150 (MQ=255)
                                                                                                                                             gCCCAGCTCTCTGGGCTGAGATTTCTATAAGATCGCACTAAATAGCTACGCGGAATAGGGCTCTACTTTCGGGTAGCCCTTTCCGAGGTAAGACCTTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGGAGCTc        >  2:259230/1‑150 (MQ=255)
                                                                                                                                                   ctctctGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTGCTCCTTTTg  <  2:629350/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGCTTATAACCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTGCTCCTTTTG  >  NC_000913/2757271‑2757567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: