Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,761,623 C→A 7.9% V640L (GTA→TTA)  yfjK ← radiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,761,6230CA 7.9% 354.0 / 17.6 126V640L (GTA→TTA) yfjKradiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage
Reads supporting (aligned to +/- strand):  ref base C (64/52);  new base A (3/7);  total (67/59)
Fisher's exact test for biased strand distribution p-value = 1.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

Only 100 of 127 total aligned reads displayed.

GGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCATAATCG  >  NC_000913/2761474‑2761771
                                                                                                                                                     |                                                                                                                                                    
ggAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTAc                                                                                                                                                      >  2:864615/1‑150 (MQ=255)
 gAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACa                                                                                                                                                     >  2:1568019/1‑150 (MQ=255)
     aTTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgt                                                                                                                                                 <  2:1524494/150‑1 (MQ=255)
     aTTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgt                                                                                                                                                 >  1:914475/1‑150 (MQ=255)
      ttCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGTAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgtg                                                                                                                                                >  2:577048/1‑150 (MQ=255)
       tCCAGCAATTCTAGCAGTCTGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTATGGCATTGACTCCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGAGTATACCACGCTTTACAGCgtgg                                                                                                                                               <  1:85468/150‑1 (MQ=255)
        ccAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATAGACTTTGCTGCGTATACCACGCTTTACAGCgtggt                                                                                                                                              <  2:428597/150‑1 (MQ=255)
        ccAGCAACTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCATCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGGATACCACGCTTTACAGCgtggt                                                                                                                                              <  2:1178948/150‑1 (MQ=255)
         cAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACAACGCTTTACAGCgtggtg                                                                                                                                             >  2:1707875/1‑150 (MQ=255)
          aGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggtat                                                                                                                                            <  2:386299/150‑3 (MQ=255)
           gCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGtt                                                                                                                                           >  1:1007558/1‑150 (MQ=255)
            cAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggtatac                                                                                                                                          <  1:1446569/150‑5 (MQ=255)
            cAAGTCTACCAGTCGGTGAAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGCAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggtatac                                                                                                                                          <  1:71165/150‑5 (MQ=255)
             aaGTCTACCAGGCGGGGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTa                                                                                                                                         <  2:389588/150‑1 (MQ=255)
              aGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggtatacgc                                                                                                                                        <  1:47492/150‑7 (MQ=255)
              aGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGGGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggtatacgc                                                                                                                                        <  1:1283704/150‑7 (MQ=255)
               gTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAAc                                                                                                                                       <  1:1707875/150‑1 (MQ=255)
                 cTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTa                                                                                                                                     >  1:1392914/1‑150 (MQ=255)
                 cTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTa                                                                                                                                     >  2:1701363/1‑150 (MQ=255)
                  tACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTAc                                                                                                                                    >  1:1530973/1‑150 (MQ=255)
                  tACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTAc                                                                                                                                    >  2:251763/1‑150 (MQ=255)
                    ccAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAt                                                                                                                                  >  1:27981/1‑150 (MQ=255)
                    ccAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGAATTTGCAGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAt                                                                                                                                  >  2:415633/1‑150 (MQ=255)
                    ccAGTCGGTGTAAAGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGGGGTAGATGGAAACTATAGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTATACAGCGTGGTGTTTAACTACAt                                                                                                                                  >  1:1087090/1‑150 (MQ=255)
                     cAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAtc                                                                                                                                 <  1:25569/150‑1 (MQ=255)
                      aGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAtcc                                                                                                                                <  2:667026/150‑1 (MQ=255)
                         cGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAtcctcc                                                                                                                             >  2:1540469/1‑150 (MQ=255)
                         cGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAtcctcc                                                                                                                             <  2:689645/150‑1 (MQ=255)
                          ggTGTAACGTCTGAATGGGAATGCCATTCTCTTCTAAGGCATTGACACCTGGAGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGAGGTGTTTAACTAGATCCTCCa                                                                                                                            >  1:830604/1‑150 (MQ=255)
                          ggTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCa                                                                                                                            >  2:1724558/1‑150 (MQ=255)
                          ggTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCa                                                                                                                            >  2:373890/1‑150 (MQ=255)
                          ggTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCa                                                                                                                            <  1:33226/150‑1 (MQ=255)
                                   tCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGa                                                                                                                   <  2:1304919/150‑1 (MQ=255)
                                     tGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGc                                                                                                                 >  2:425502/1‑150 (MQ=255)
                                     tGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGc                                                                                                                 <  1:1197652/150‑1 (MQ=255)
                                          gggAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCtt                                                                                                            <  2:402708/150‑1 (MQ=255)
                                            gAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtag                                                                                                          >  1:183865/1‑150 (MQ=255)
                                            gAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtag                                                                                                          <  2:1407939/150‑1 (MQ=255)
                                               tGCCACTCTCTTCTACGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggta                                                                                                       <  2:665427/150‑1 (MQ=255)
                                                  cAATCTCTTCTAATGCATTGACAACTGGTTGTAGATGGAAACTTTCGAACGCCAATTTGAGATGTGTGTAATCGACTTTGCTGCGTATACCACGATTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAggg                                                                                                    <  2:694185/150‑1 (MQ=255)
                                                  aaaTCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTTACTTGTGTGTTATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTTACTACATCCTGCATCAAGCTGAGCACCTTAGGTAggg                                                                                                    <  2:1087090/149‑1 (MQ=255)
                                                    aTCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGta                                                                                                  >  1:941806/1‑150 (MQ=255)
                                                       tcttctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGAGTTTGCTGAGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAATCTGAGCAACTTAGGTAGGGTATAg                                                                                               >  1:254365/1‑150 (MQ=255)
                                                       tcttctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAg                                                                                               >  1:1566680/1‑150 (MQ=255)
                                                       tcttctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAg                                                                                               <  1:1103260/150‑1 (MQ=255)
                                                       tcttctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAg                                                                                               <  1:864615/150‑1 (MQ=255)
                                                            tAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGaa                                                                                          >  2:211327/1‑150 (MQ=255)
                                                             aaGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAg                                                                                         <  1:373890/150‑1 (MQ=255)
                                                              aGGCATTGACAGCTGGTTGTAGATGGAAACTTTAGAACGCCAATTTGACTTGGGGGTAATCGACTTTGCTGCGTATTCCACGCTTTACCGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTATGGTATAGCCGAAgg                                                                                        <  2:527948/150‑1 (MQ=255)
                                                               ggCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCCCGCTTTACAGCGTGGTGTTGAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGt                                                                                       >  2:73284/1‑150 (MQ=255)
                                                               ggCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCAGAAGGt                                                                                       >  1:33981/1‑150 (MQ=255)
                                                                  aTTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTAtt                                                                                    >  1:778095/1‑150 (MQ=255)
                                                                    tGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATTCCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTgg                                                                                  <  2:398275/150‑1 (MQ=255)
                                                                    tGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTgg                                                                                  <  1:1158699/150‑1 (MQ=255)
                                                                     gACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGt                                                                                 <  2:1564829/150‑1 (MQ=255)
                                                                       cacCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTa                                                                               <  2:1234971/150‑1 (MQ=255)
                                                                        acCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTAt                                                                              >  1:197025/1‑150 (MQ=255)
                                                                         ccTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACCGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATg                                                                             >  1:1309405/1‑150 (MQ=255)
                                                                                gtAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCa                                                                      >  1:1173222/1‑150 (MQ=255)
                                                                                 tAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCaa                                                                     >  2:1488957/1‑150 (MQ=255)
                                                                                   gATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAAt                                                                   >  1:1503866/1‑150 (MQ=255)
                                                                                        aaaCTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttg                                                              >  2:887432/1‑150 (MQ=255)
                                                                                               cGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAggg                                                       <  2:1338806/150‑1 (MQ=255)
                                                                                                gAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGACGATGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGc                                                      >  1:1057056/1‑150 (MQ=255)
                                                                                                 aaCGCAAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTTAGGGCa                                                     >  1:1112255/1‑150 (MQ=255)
                                                                                                        aTTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGaaa                                              >  1:1499582/1‑150 (MQ=255)
                                                                                                        aTTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGaaa                                              <  2:1377189/150‑1 (MQ=255)
                                                                                                         tttGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAag                                             <  2:250031/150‑1 (MQ=255)
                                                                                                           tGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAagag                                           >  2:106271/1‑150 (MQ=255)
                                                                                                             aCATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTa                                         <  2:1616412/150‑1 (MQ=255)
                                                                                                               aTGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACg                                       <  2:443480/150‑1 (MQ=255)
                                                                                                                tgtgtgTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGt                                      >  2:1449139/1‑150 (MQ=255)
                                                                                                                 gtgtgtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGTACCGTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGtt                                     >  2:930490/1‑150 (MQ=255)
                                                                                                                 gtgtgtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGtt                                     <  1:1527517/150‑1 (MQ=255)
                                                                                                                  tgtgtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTGCAAATCATTGTTGAGGGCAATGGAAAGAGTACGttt                                    >  2:45467/1‑150 (MQ=255)
                                                                                                                   gtgtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTtc                                   >  1:444721/1‑150 (MQ=255)
                                                                                                                     gtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTtctc                                 >  1:916908/1‑150 (MQ=255)
                                                                                                                       aaTCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGACGGCAATGTAAAGAGTTCGTTTCTccc                               >  2:300427/1‑150 (MQ=255)
                                                                                                                        aTCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGACGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTcccc                              <  1:1363067/150‑1 (MQ=255)
                                                                                                                        aTCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTcccc                              >  2:1180040/1‑150 (MQ=255)
                                                                                                                        aTCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTcccc                              >  2:511067/1‑150 (MQ=255)
                                                                                                                        aTCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTcccc                              >  1:709354/1‑150 (MQ=255)
                                                                                                                        aTCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTGAGGTAGGGTATAGCCGATGGTATTGGTTATGAGCTTCAAATCATTGTTAATGGCAATGGAAATAGTACGTTTCTcccc                              >  1:335415/1‑150 (MQ=255)
                                                                                                                           gACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTc                           <  1:251763/150‑1 (MQ=255)
                                                                                                                           gACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTc                           >  1:162465/1‑150 (MQ=255)
                                                                                                                            aCTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCa                          <  1:420618/150‑1 (MQ=255)
                                                                                                                            aCTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCa                          <  2:231407/150‑1 (MQ=255)
                                                                                                                                   tgcGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAACCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGGTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATcc                   <  1:837792/150‑1 (MQ=255)
                                                                                                                                   gttaaacaCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATcc                   >  1:404988/8‑150 (MQ=255)
                                                                                                                                     taaacaCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTAAGGAAGGGTATATCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCAc                 >  2:593204/6‑150 (MQ=255)
                                                                                                                                      gTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACt                >  1:479102/1‑150 (MQ=255)
                                                                                                                                          aCCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTATGTTTCTCCCCTTCAGAAATCCACTGTCg            <  1:455105/150‑1 (MQ=255)
                                                                                                                                           ccACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGt           >  2:641542/1‑150 (MQ=255)
                                                                                                                                           ccACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGt           >  1:1125289/1‑150 (MQ=255)
                                                                                                                                           ccACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATAAAGATGAGCACCGTAGGTAGGGGTAAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTAAGTTTCTCCCCTTCAGAAATACACTGTCGt           >  2:688725/1‑150 (MQ=255)
                                                                                                                                            cACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTg          <  1:1040798/150‑1 (MQ=255)
                                                                                                                                              cGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCa        <  1:1180040/150‑1 (MQ=255)
                                                                                                                                              cGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCa        <  1:511067/150‑1 (MQ=255)
                                                                                                                                                   tACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCATAATc   >  1:1005292/1‑150 (MQ=255)
                                                                                                                                                    aCAGCGTGGTGTTTAACTACATCCTCCATAAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGATCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCATAATCg  <  1:73284/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCATAATCG  >  NC_000913/2761474‑2761771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: