Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,777,571 A→G 8.4% D40G (GAC→GGC)  ypjF → CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,777,5710AG 8.4% 328.2 / 17.2 121D40G (GAC→GGC) ypjFCP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base A (51/58);  new base G (6/4);  total (58/63)
Fisher's exact test for biased strand distribution p-value = 5.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-02

Only 100 of 121 total aligned reads displayed.

ACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATT  >  NC_000913/2777426‑2777719
                                                                                                                                                 |                                                                                                                                                    
aCGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACg                                                                                                                                                  <  1:973329/150‑1 (MQ=255)
   ctaactaaTTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCg                                                                                                                                               <  2:1195957/150‑1 (MQ=255)
     aactaaTTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGCCACGCCAGCTGGAACCACACTATGGCCTGACACTGACCGACACGCCGtt                                                                                                                                             >  2:882999/1‑150 (MQ=255)
      actaaTTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGAAAATGCTACTGACACTCCTGCTGGAACAACACTATTGAATGACACTGAACGACACGCCGTTc                                                                                                                                            <  2:918115/150‑1 (MQ=255)
        taaTTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAg                                                                                                                                          <  1:174393/150‑1 (MQ=255)
         aaTTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGGCGTGTCGTTCAGt                                                                                                                                         <  2:80461/150‑1 (MQ=255)
          aTTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGGCGTGTCGTTCAGtg                                                                                                                                        <  1:1029774/150‑1 (MQ=255)
            ttCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGtgat                                                                                                                                      >  2:826349/1‑150 (MQ=255)
             tCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGtgatg                                                                                                                                     <  1:436140/150‑1 (MQ=255)
                 gagaGCAAGCATTAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCAACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAAc                                                                                                                                 <  2:142390/150‑1 (MQ=255)
                 gagaGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAAc                                                                                                                                 <  2:519068/150‑1 (MQ=255)
                   gagCAAGCATGAACACTCTACCTGCTACAATTTCGCCGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGGCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACGATGGCCTGACACTGCACGACACGCCGGTCAGTGATGCAACTg                                                                                                                               >  2:393236/1‑150 (MQ=255)
                    agcaagcaTGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCTTTCAGTGATGAAACTGt                                                                                                                              <  2:569579/150‑1 (MQ=255)
                     gcaagcaTGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGTCAGTGGCTGTCTGTCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGATCAGTGATGAAACTGtt                                                                                                                             <  1:986692/150‑1 (MQ=255)
                       aagcaTGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGttat                                                                                                                           >  1:489588/1‑150 (MQ=255)
                        agcaTGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACCCGCCGTTCAGTGATGAAACTGttatt                                                                                                                          >  1:609224/1‑150 (MQ=255)
                           aTGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAg                                                                                                                       <  1:1005736/150‑1 (MQ=255)
                            tGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACCCGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAgg                                                                                                                      >  2:1601916/1‑150 (MQ=255)
                                cacTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACa                                                                                                                  <  1:827831/150‑1 (MQ=255)
                                cacTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGCGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACa                                                                                                                  <  2:618093/150‑1 (MQ=255)
                                  ctctACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACata                                                                                                                >  2:806034/1‑150 (MQ=255)
                                     tACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCg                                                                                                             <  2:1525460/150‑1 (MQ=255)
                                        cTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATg                                                                                                          <  2:1182387/150‑1 (MQ=255)
                                        cTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGATGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATg                                                                                                          <  2:1611171/150‑1 (MQ=255)
                                          gCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCt                                                                                                        <  2:1332939/150‑1 (MQ=255)
                                           cTACAAATTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGTCAGTCTGGCAAAAGCTACTGACACGCCTACTGGAACAACACTATGGCCTGACACTGAACGACTCGCCGTGCAGTGATGCCACTGTTATTAAGTAACATATCGctgctg                                                                                                       >  1:1039397/1‑150 (MQ=255)
                                              cAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGtt                                                                                                    >  1:1164836/1‑149 (MQ=255)
                                              cAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTa                                                                                                    >  2:1324631/1‑150 (MQ=255)
                                               aaTTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTAt                                                                                                   >  2:1275455/1‑150 (MQ=255)
                                                    cGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCActc                                                                                              <  1:933774/150‑1 (MQ=255)
                                                     gCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGGTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCActct                                                                                             >  2:823844/1‑150 (MQ=255)
                                                        ggcggcGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGc                                                                                          <  1:1524422/150‑1 (MQ=255)
                                                          cggcgAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCg                                                                                        >  1:1493370/1‑150 (MQ=255)
                                                            gcgAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGAt                                                                                      >  2:1493094/1‑150 (MQ=255)
                                                            gcgAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGAt                                                                                      >  1:456287/1‑150 (MQ=255)
                                                             cgAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATg                                                                                     <  1:677449/150‑1 (MQ=255)
                                                              gAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGc                                                                                    <  1:257410/150‑1 (MQ=255)
                                                                  cccTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTTTTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTc                                                                                <  2:845924/150‑1 (MQ=255)
                                                                   cctgcctgTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCTGATGCAGTCa                                                                               >  1:1640195/1‑150 (MQ=255)
                                                                       cctgTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAAttt                                                                           <  2:489588/150‑1 (MQ=255)
                                                                          gTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCt                                                                        <  2:1200542/150‑1 (MQ=255)
                                                                          gTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCt                                                                        >  1:1360139/1‑150 (MQ=255)
                                                                            ggCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCtgg                                                                      <  1:927302/149‑1 (MQ=255)
                                                                            cGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCtgg                                                                      <  2:319629/150‑1 (MQ=255)
                                                                               cAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCtggtgg                                                                   >  2:710854/1‑150 (MQ=255)
                                                                                 gTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGaa                                                                 >  1:673818/1‑150 (MQ=255)
                                                                                  tGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGaaa                                                                <  1:463864/150‑1 (MQ=255)
                                                                                   ggCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGaaaa                                                               <  1:826349/150‑1 (MQ=255)
                                                                                    gCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACTCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAg                                                              <  1:1306971/150‑1 (MQ=255)
                                                                                    gCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAg                                                              <  1:1634413/150‑1 (MQ=255)
                                                                                      tGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTa                                                            >  1:687235/1‑150 (MQ=255)
                                                                                        tCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACg                                                          <  1:1283975/150‑1 (MQ=255)
                                                                                        tCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACg                                                          <  1:989653/150‑1 (MQ=255)
                                                                                        tCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACg                                                          >  1:1206232/1‑150 (MQ=255)
                                                                                         cTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGa                                                         >  2:713805/1‑150 (MQ=255)
                                                                                           ggCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGTTGGAAAAGTACGAAc                                                       >  2:1145790/1‑150 (MQ=255)
                                                                                           ggCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAAc                                                       >  2:680269/1‑150 (MQ=255)
                                                                                           ggCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAAc                                                       >  1:1263244/1‑150 (MQ=255)
                                                                                            gCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAACGTACGAACt                                                      >  2:722953/1‑150 (MQ=255)
                                                                                              aaaTGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTgg                                                    <  1:1252217/150‑1 (MQ=255)
                                                                                                aTGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTa                                                  <  1:1140534/150‑1 (MQ=255)
                                                                                                aTGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTa                                                  <  2:946566/150‑1 (MQ=255)
                                                                                                aTGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTa                                                  <  2:1172141/150‑1 (MQ=255)
                                                                                                  gCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACg                                                <  2:691538/150‑1 (MQ=255)
                                                                                                   cTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGt                                               >  1:195861/1‑150 (MQ=255)
                                                                                                    tACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTa                                              <  2:326570/150‑1 (MQ=255)
                                                                                                     aCTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACCCGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGACCTGGTACGTAt                                             >  1:1081734/1‑150 (MQ=255)
                                                                                                     aCTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGGGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGACCTGGTACGTAt                                             >  1:1087348/1‑150 (MQ=255)
                                                                                                       tGACACGCCTGCTGGAACAACAGTATGGCCTGACACTGAACGTCACGCCGGTCAGTGATGTAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCg                                           <  2:1337862/150‑1 (MQ=255)
                                                                                                         acacGCCTGCTGGAACAACACTAGGGCCTGACACAGACCTACACGCCGTTCAGTGATGAAACTTTTATTAAGTAACATAACGATGCTGGTATCAATCTGGCCGATGCAGACAATAATCTGGTGGAAAAGTACGAACTGGTACGTATCGAt                                         <  2:882662/150‑1 (MQ=255)
                                                                                                          cacGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATc                                        >  2:1706930/1‑150 (MQ=255)
                                                                                                          cacGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATACCTCTGGCCGATGCAGTCAATTTTCTGGTGGATAAGTAAGAACTGGTACGTATCGATc                                        >  2:210134/1‑150 (MQ=255)
                                                                                                          cacGCCAGCTGGAACAACACTATGGCCTGACACTGAACGCCACGCCGTTCAGTGATGAAACTCTTATTAAGGAACATATCGATGCTGGTCTCACTCAGGCCGATGCAGTCAATTTTCTGTTGGAAAAGTACGAACTGGTACGTATCGATc                                        >  1:82118/1‑150 (MQ=255)
                                                                                                          cacGACTGCTGGAACAAAACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATc                                        <  1:37655/150‑1 (MQ=255)
                                                                                                             gCCTGCTGGAACAACACTATGGCCTGACACTGAACGACTCGCCGTTCAGTGTTGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATcaca                                     <  1:1697847/150‑1 (MQ=255)
                                                                                                              ccTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACag                                    >  1:546994/1‑150 (MQ=255)
                                                                                                                 gctgGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAgg                                 <  1:565423/150‑1 (MQ=255)
                                                                                                                    ggAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGAtt                              >  1:1472846/1‑150 (MQ=255)
                                                                                                                        aaacaCTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCg                          <  1:255316/149‑1 (MQ=255)
                                                                                                                         aacaCTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGAGTTTCGt                         >  2:236365/1‑150 (MQ=255)
                                                                                                                          acacTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGATGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTg                        >  1:1136672/1‑150 (MQ=255)
                                                                                                                           cacTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTgg                       <  1:1601916/150‑1 (MQ=255)
                                                                                                                               aTGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGcaac                   >  1:544572/1‑150 (MQ=255)
                                                                                                                                tGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGAATTTCGTGGcaaca                  >  2:1024185/1‑150 (MQ=255)
                                                                                                                                 ggCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGcaacaa                 <  1:1586886/150‑1 (MQ=255)
                                                                                                                                    tcatcacTGAACGGCGTGTCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAg              >  1:977452/5‑150 (MQ=255)
                                                                                                                                    cTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAg              <  1:353879/150‑1 (MQ=255)
                                                                                                                                       tcacTGAACGGCGTGTCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGtct           >  1:1458501/2‑150 (MQ=255)
                                                                                                                                        cacTGAACGGCGTGTCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTCGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGtctc          >  2:201028/1‑150 (MQ=255)
                                                                                                                                         acTGAACGGCGTGTCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTcc         >  1:1119181/1‑150 (MQ=255)
                                                                                                                                         acTGAACGGCGTGTCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTcc         >  2:1277389/1‑150 (MQ=255)
                                                                                                                                         acTGAACGGCGTGTCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTcc         >  2:1289469/1‑150 (MQ=255)
                                                                                                                                         acTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTcc         <  2:529216/150‑1 (MQ=255)
                                                                                                                                         acTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTcc         <  1:588018/150‑1 (MQ=255)
                                                                                                                                           tGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCat       >  2:1125506/1‑150 (MQ=255)
                                                                                                                                           tGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCat       <  1:1489058/150‑1 (MQ=255)
                                                                                                                                           tGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCat       >  2:1154302/1‑150 (MQ=255)
                                                                                                                                            gAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCata      >  2:1362157/1‑150 (MQ=255)
                                                                                                                                                gACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATAtt  >  2:343513/1‑150 (MQ=255)
                                                                                                                                                gACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATAtt  >  2:1699418/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCGATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATT  >  NC_000913/2777426‑2777719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: