Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,788,671 A→T 5.9% intergenic (+22/‑314) ygaQ → / → csiD uncharacterized protein; putative enzyme/carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,788,6710AT 5.9% 379.7 / 5.9 129intergenic (+22/‑314)ygaQ/csiDuncharacterized protein; putative enzyme/carbon starvation protein
Reads supporting (aligned to +/- strand):  ref base A (52/68);  new base T (4/4);  total (56/73)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

CCGAAAAATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCC  >  NC_000913/2788523‑2788820
                                                                                                                                                    |                                                                                                                                                     
ccGAAAAATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGAt                                                                                                                                                      <  1:631264/150‑1 (MQ=255)
ccGAAAAATTATCCCCACTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGAt                                                                                                                                                      <  2:281800/150‑1 (MQ=255)
ccGAAAAATTATCCCACCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGAt                                                                                                                                                      >  1:1121205/1‑150 (MQ=255)
  gAAAAATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATaa                                                                                                                                                    >  1:587261/1‑150 (MQ=255)
   aaaaaTTATCCCCCCTGGGAAAGTATTTCCGCTTAGAGGCGGAACGCTAAATATCAAGATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAAACGCCGAACTATCTCTATTTATAATTTTATGTTATATCTGCCGCGATaaa                                                                                                                                                   >  2:264487/1‑150 (MQ=255)
      aaTTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACg                                                                                                                                                >  2:852125/1‑150 (MQ=255)
      aaTTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGAAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATTATTTAATGTTATATCTGCCCCGATAAAACg                                                                                                                                                >  2:275856/1‑150 (MQ=255)
      aaTTATCACCCCTGGTAAAGTATTTACGATTAGAGGCTGAACTCTAAATATCAATATTCCTGGGAGTAGCTCTCTTCTTTTATGGAAAACAGGAGAACCGCCTAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACg                                                                                                                                                <  1:295951/150‑1 (MQ=255)
        ttATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACggg                                                                                                                                              <  2:1027100/150‑1 (MQ=255)
           tCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGTTTTATCGGGGCa                                                                                                                                           <  1:126485/150‑1 (MQ=255)
            ccccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGTTTTATCGGGGCAg                                                                                                                                          <  2:1324932/150‑1 (MQ=255)
             cccccTGGTAAAGTATTTACGCTTAGAGTCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACAATCTCTATTTATAATTTAATGTTAAATCTGCCCAGATAAAACCGGGCAGa                                                                                                                                         >  2:255047/1‑150 (MQ=255)
             cccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGTTTTATCGGGGCAGa                                                                                                                                         <  1:376932/150‑1 (MQ=255)
             cccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGa                                                                                                                                         <  1:1382490/150‑1 (MQ=255)
             cccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGa                                                                                                                                         >  2:685486/1‑150 (MQ=255)
             cccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTATTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATTTTATATCTGCCCCGATAAAACGGGGCAGa                                                                                                                                         <  1:1518476/150‑1 (MQ=255)
              ccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGat                                                                                                                                        >  2:141709/1‑150 (MQ=255)
              ccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGat                                                                                                                                        <  2:1225383/150‑1 (MQ=255)
                   ggTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAatat                                                                                                                                   >  1:195089/1‑150 (MQ=255)
                     tAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTAAAATTTATTGTTATATCTGCCCCGTTTTATCGGGGCAGatataacat                                                                                                                                 <  1:106167/150‑7 (MQ=255)
                      aaaGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATGTTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtt                                                                                                                                >  2:887784/1‑150 (MQ=255)
                         gTATTTACGCTTAGAGGCGGTACTATAAATATCAATAATCCTGGGCGTAGCGCTCTTATTTTAGGGAAAACAGGAGAACCGACGAACTATCTCTATTTATAAATTTATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtttag                                                                                                                             <  2:350921/150‑1 (MQ=255)
                          tATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtttagt                                                                                                                            >  1:782959/1‑150 (MQ=255)
                           aTTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAACACAGGAGAACCGCCGAACTATCTCTATTGATAATTTAATGTTATATCTGCCCCGATAAAACGGGTCAGATAATATgtttagtt                                                                                                                           >  1:133109/1‑150 (MQ=255)
                           aTTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtttagtt                                                                                                                           <  1:986698/150‑1 (MQ=255)
                                 gCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAATACAGGAGAAACGACGAACTATCTCTATTTATAATTTAATGTTATATCTGAACCGATAAAAAGGGGCAGATAATATGTTTAGTTTACTaa                                                                                                                     >  1:707625/1‑150 (MQ=255)
                                     agagGCGGAACTCTAAATATGAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACATGAGAACCTCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGt                                                                                                                 <  1:1537379/150‑1 (MQ=255)
                                     agagGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTGTAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGt                                                                                                                 >  2:1596649/1‑150 (MQ=255)
                                     agagGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTCACTAACGGt                                                                                                                 <  2:1429755/150‑1 (MQ=255)
                                        ggCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCAt                                                                                                              >  1:1331126/1‑150 (MQ=255)
                                           ggAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCAtttt                                                                                                           <  1:1617766/150‑1 (MQ=255)
                                           ggAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCAtttt                                                                                                           >  2:1689475/1‑150 (MQ=255)
                                             aaCTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTCGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGc                                                                                                         <  2:1518572/150‑1 (MQ=255)
                                             aaCTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTGTAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGc                                                                                                         <  1:1596649/150‑1 (MQ=255)
                                             aaCTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGc                                                                                                         <  2:804805/150‑1 (MQ=255)
                                              aCTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCa                                                                                                        <  2:782959/150‑1 (MQ=255)
                                              aCTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCa                                                                                                        <  1:673693/150‑1 (MQ=255)
                                                tctAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGt                                                                                                      >  1:761565/1‑150 (MQ=255)
                                                  tAAATATCAATAATCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGa                                                                                                    <  2:1357246/150‑1 (MQ=255)
                                                      tatCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGcc                                                                                                >  1:342331/1‑150 (MQ=255)
                                                         cAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCAtt                                                                                             >  2:1603740/1‑150 (MQ=255)
                                                         cAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGACGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCAtt                                                                                             >  2:1672271/1‑150 (MQ=255)
                                                          aaTATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCAttt                                                                                            <  2:1073967/150‑1 (MQ=255)
                                                          aaTATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCAttt                                                                                            <  1:758438/150‑1 (MQ=255)
                                                            tatTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTAc                                                                                          <  1:1629272/150‑1 (MQ=255)
                                                              ttCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTg                                                                                        <  2:10922/150‑1 (MQ=255)
                                                                 cTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGttt                                                                                     <  1:573332/150‑1 (MQ=255)
                                                                  tGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAA‑TTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGttttt                                                                                   <  2:838394/150‑1 (MQ=255)
                                                                   gggCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAAGCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGttttt                                                                                   >  1:1087566/1‑150 (MQ=255)
                                                                   gggCGTAGCGCTCTTCATTTAGCGAAAACAGCATAACCGCCGAACTATCTCTATTTATAATTTAATGTTAAATCTGCCCCGATAAAACGCGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGttttt                                                                                   <  2:170608/150‑1 (MQ=255)
                                                                        tatctctctTCTTTTAGGGATAACTGGAGAACCGACGAACTATCTCTAATTATAATTTAATGTTAAATCAGCCCCGATAAAACGGGGCAGATATTATGTTTAGTTTACTAACTGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCg                                                                              <  2:677663/145‑1 (MQ=255)
                                                                        tAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCg                                                                              <  2:1645085/150‑1 (MQ=255)
                                                                        actcgcTCTTCTTTTAGGGAAAACAGTAGAACCGCCGACCTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCg                                                                              <  2:137119/147‑1 (MQ=255)
                                                                            gcTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTTTTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCa                                                                          <  1:1615667/150‑1 (MQ=255)
                                                                            gcTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCa                                                                          >  2:1656612/1‑150 (MQ=255)
                                                                             ctctTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAg                                                                         <  1:379198/150‑1 (MQ=255)
                                                                              tcttctTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGa                                                                        >  1:1624627/1‑150 (MQ=255)
                                                                               cttcttTTAGGTAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGAt                                                                       <  2:1069590/150‑1 (MQ=255)
                                                                                  cttTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGTTAAt                                                                    >  2:841294/1‑150 (MQ=255)
                                                                                  cttTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAAt                                                                    >  2:1434471/1‑150 (MQ=255)
                                                                                  cttTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAAt                                                                    >  1:969796/1‑150 (MQ=255)
                                                                                  cttTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAAt                                                                    >  2:308670/1‑150 (MQ=255)
                                                                                   ttttAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAAta                                                                   >  1:416550/1‑149 (MQ=255)
                                                                                    tttAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCt                                                                  >  2:74381/1‑150 (MQ=255)
                                                                                      tAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGt                                                                <  2:626960/150‑1 (MQ=255)
                                                                                       tgggAAAACAGGAGAACCGCCGAACTATCTATATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGtt                                                               <  2:1450275/149‑1 (MQ=255)
                                                                                       aGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGtt                                                               <  2:1729552/150‑1 (MQ=255)
                                                                                        gggAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTtc                                                              <  2:682286/150‑1 (MQ=255)
                                                                                         ggAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTtct                                                             >  1:931748/1‑150 (MQ=255)
                                                                                         ggAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTtct                                                             >  2:1371495/1‑150 (MQ=255)
                                                                                          gAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTtcta                                                            >  2:819908/1‑149 (MQ=255)
                                                                                             aaCAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTaa                                                         <  1:719111/150‑1 (MQ=255)
                                                                                              aCAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAAt                                                        >  2:606891/1‑150 (MQ=255)
                                                                                               cAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAAtt                                                       >  2:1454892/1‑149 (MQ=255)
                                                                                                aGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGt                                                      <  2:719428/150‑1 (MQ=255)
                                                                                                 ggAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACCGTTTTTTATCGACCAGATAATCTGTTCTCTAATGtt                                                     <  2:1483795/150‑1 (MQ=255)
                                                                                                                tATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCACTAACCt                                      >  1:301400/1‑150 (MQ=255)
                                                                                                                   ctctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGtt                                   <  1:1689475/150‑1 (MQ=255)
                                                                                                                    tctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTAATAAAGTTAATTTTGCAGTGAAGACATTTACTGTTTTTTATCGACCAGATAATCTTTTATCTAATGTTAACTCACCCTAACCTGttt                                  >  1:72576/1‑149 (MQ=255)
                                                                                                                     ctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGc                                 >  2:10877/1‑150 (MQ=255)
                                                                                                                     ctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGc                                 >  2:465663/1‑150 (MQ=255)
                                                                                                                        tttATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCttt                              <  2:342331/150‑1 (MQ=255)
                                                                                                                         ttATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTATCGGTCATTTTGCCGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTa                             <  2:1104840/150‑1 (MQ=255)
                                                                                                                         ttATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTa                             >  1:1397468/1‑150 (MQ=255)
                                                                                                                           ataATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGt                           <  2:587261/150‑1 (MQ=255)
                                                                                                                           ataATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCAGTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGt                           >  1:266414/1‑150 (MQ=255)
                                                                                                                            taATTTAATGTTATATCTGCCACGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGtt                          <  1:887784/150‑1 (MQ=255)
                                                                                                                             aaTTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACTGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATGAGTTCTCTAATGTTAACTACCCCTAACCTGTTGCTTTAGTTa                         >  1:1127809/1‑150 (MQ=255)
                                                                                                                                    tGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCAttt                  >  1:1426684/1‑150 (MQ=255)
                                                                                                                                      ttATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTATTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCACCTAACCTGTTGCTTTAGTTATTCATTTcc                <  2:145343/150‑1 (MQ=255)
                                                                                                                                      ttATATCTGCCCCGATAAAAAGGGTCAGATAATATGTTTAGTTTACTAACGTTAATTTTGCAGTGAAGCCATTTACTTTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTcc                <  2:707625/150‑1 (MQ=255)
                                                                                                                                        ttatCTGCCCCGTTTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTg              >  1:966851/2‑150 (MQ=255)
                                                                                                                                        ttatCTGCCCCGTTTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTg              >  2:100827/2‑150 (MQ=255)
                                                                                                                                        atatCAGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTg              <  1:852125/150‑1 (MQ=255)
                                                                                                                                          atCTGCCCCGTTTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGtc            >  2:247967/1‑150 (MQ=255)
                                                                                                                                          atCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGtc            >  2:1455664/1‑150 (MQ=255)
                                                                                                                                          atCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGtc            <  2:1331126/150‑1 (MQ=255)
                                                                                                                                          atCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTAATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATTATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGtc            >  2:928121/1‑150 (MQ=255)
                                                                                                                                           tCTGCCCCGTTTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTACTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGtct           >  2:1555799/1‑150 (MQ=255)
                                                                                                                                            cTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCACATAACCTGTGGCTTTAGTTATTCATTTCCTGtcta          >  2:835631/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCGAAAAATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCC  >  NC_000913/2788523‑2788820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑