Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,788,673 A→T 6.3% intergenic (+24/‑312) ygaQ → / → csiD uncharacterized protein; putative enzyme/carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,788,6730AT 6.3% 354.2 / 8.7 125intergenic (+24/‑312)ygaQ/csiDuncharacterized protein; putative enzyme/carbon starvation protein
Reads supporting (aligned to +/- strand):  ref base A (48/69);  new base T (4/4);  total (52/73)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

Only 100 of 128 total aligned reads displayed.

GAAAAATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCCTT  >  NC_000913/2788525‑2788822
                                                                                                                                                    |                                                                                                                                                     
gAAAAATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATaa                                                                                                                                                      >  1:587261/1‑150 (MQ=255)
 aaaaaTTATCCCCCCTGGGAAAGTATTTCCGCTTAGAGGCGGAACGCTAAATATCAAGATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAAACGCCGAACTATCTCTATTTATAATTTTATGTTATATCTGCCGCGATaaa                                                                                                                                                     >  2:264487/1‑150 (MQ=255)
    aaTTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACg                                                                                                                                                  >  2:852125/1‑150 (MQ=255)
    aaTTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGAAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATTATTTAATGTTATATCTGCCCCGATAAAACg                                                                                                                                                  >  2:275856/1‑150 (MQ=255)
    aaTTATCACCCCTGGTAAAGTATTTACGATTAGAGGCTGAACTCTAAATATCAATATTCCTGGGAGTAGCTCTCTTCTTTTATGGAAAACAGGAGAACCGCCTAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACg                                                                                                                                                  <  1:295951/150‑1 (MQ=255)
        aTCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGc                                                                                                                                              <  1:1174567/150‑1 (MQ=255)
           cccccTGGTAAAGTATTTACGCTTAGAGTCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACAATCTCTATTTATAATTTAATGTTAAATCTGCCCAGATAAAACCGGGCAGa                                                                                                                                           >  2:255047/1‑150 (MQ=255)
           cccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGTTTTATCGGGGCAGa                                                                                                                                           <  1:376932/150‑1 (MQ=255)
           cccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGa                                                                                                                                           <  1:1382490/150‑1 (MQ=255)
           cccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGa                                                                                                                                           >  2:685486/1‑150 (MQ=255)
           cccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTATTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATTTTATATCTGCCCCGATAAAACGGGGCAGa                                                                                                                                           <  1:1518476/150‑1 (MQ=255)
            ccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGat                                                                                                                                          <  2:1225383/150‑1 (MQ=255)
            ccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGat                                                                                                                                          >  1:954852/1‑150 (MQ=255)
            ccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGat                                                                                                                                          >  2:141709/1‑150 (MQ=255)
                   tAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTAAAATTTATTGTTATATCTGCCCCGTTTTATCGGGGCAGatataacat                                                                                                                                   <  1:106167/150‑7 (MQ=255)
                    aaaGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATGTTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtt                                                                                                                                  >  2:887784/1‑150 (MQ=255)
                      aGTATTTTCGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGTCGTAGCGCTCTTCTTTTAGTGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgttta                                                                                                                                <  2:290836/150‑1 (MQ=255)
                       gTATTTACGCTTAGAGGCGGTACTATAAATATCAATAATCCTGGGCGTAGCGCTCTTATTTTAGGGAAAACAGGAGAACCGACGAACTATCTCTATTTATAAATTTATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtttag                                                                                                                               <  2:350921/150‑1 (MQ=255)
                       gTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtttag                                                                                                                               <  2:1450844/150‑1 (MQ=255)
                        tATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtttagt                                                                                                                              >  1:782959/1‑150 (MQ=255)
                         aTTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAACACAGGAGAACCGCCGAACTATCTCTATTGATAATTTAATGTTATATCTGCCCCGATAAAACGGGTCAGATAATATgtttagtt                                                                                                                             >  1:133109/1‑150 (MQ=255)
                         aTTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtttagtt                                                                                                                             <  1:986698/150‑1 (MQ=255)
                               gCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAATACAGGAGAAACGACGAACTATCTCTATTTATAATTTAATGTTATATCTGAACCGATAAAAAGGGGCAGATAATATGTTTAGTTTACTaa                                                                                                                       >  1:707625/1‑150 (MQ=255)
                                   agagGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTGTAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGt                                                                                                                   >  2:1596649/1‑150 (MQ=255)
                                   agagGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTCACTAACGGt                                                                                                                   <  2:1429755/150‑1 (MQ=255)
                                      ggCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCAt                                                                                                                >  1:84171/1‑150 (MQ=255)
                                      ggCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCAt                                                                                                                >  1:1331126/1‑150 (MQ=255)
                                         ggAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCAtttt                                                                                                             >  2:1689475/1‑150 (MQ=255)
                                         ggAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCAtttt                                                                                                             <  1:1617766/150‑1 (MQ=255)
                                           aaCTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTCGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGc                                                                                                           <  2:1518572/150‑1 (MQ=255)
                                           aaCTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTGTAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGc                                                                                                           <  1:1596649/150‑1 (MQ=255)
                                           aaCTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGc                                                                                                           <  2:804805/150‑1 (MQ=255)
                                            aCTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCa                                                                                                          <  1:673693/150‑1 (MQ=255)
                                            aCTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCa                                                                                                          <  2:782959/150‑1 (MQ=255)
                                              tctAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGt                                                                                                        >  1:761565/1‑150 (MQ=255)
                                                tAAATATCAATAATCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGa                                                                                                      <  2:1357246/150‑1 (MQ=255)
                                                       cAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCAtt                                                                                               >  2:1603740/1‑150 (MQ=255)
                                                       cAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGACGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCAtt                                                                                               >  2:1672271/1‑150 (MQ=255)
                                                        aaTATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCAttt                                                                                              <  2:1073967/150‑1 (MQ=255)
                                                          tatTCCTGGGCTTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTAc                                                                                            <  2:1375646/150‑1 (MQ=255)
                                                          tatTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTAc                                                                                            <  2:1702526/150‑1 (MQ=255)
                                                          tatTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTAc                                                                                            <  1:1629272/150‑1 (MQ=255)
                                                             tCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGt                                                                                         <  1:1512872/150‑1 (MQ=255)
                                                               cTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGttt                                                                                       <  1:573332/150‑1 (MQ=255)
                                                                tGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGttttt                                                                                     <  2:838394/150‑1 (MQ=255)
                                                                 gggCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAAGCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGttttt                                                                                     >  1:1087566/1‑150 (MQ=255)
                                                                      tatctctctTCTTTTAGGGATAACTGGAGAACCGACGAACTATCTCTAATTATAATTTAATGTTAAATCAGCCCCGATAAAACGGGGCAGATATTATGTTTAGTTTACTAACTGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCg                                                                                <  2:677663/145‑1 (MQ=255)
                                                                      tAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCg                                                                                <  2:1645085/150‑1 (MQ=255)
                                                                      actcgcTCTTCTTTTAGGGAAAACAGTAGAACCGCCGACCTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCg                                                                                <  2:137119/147‑1 (MQ=255)
                                                                          gcTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTTTTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCa                                                                            <  1:1615667/150‑1 (MQ=255)
                                                                          gcTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCa                                                                            >  2:1656612/1‑150 (MQ=255)
                                                                           ctctTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAg                                                                           <  1:379198/150‑1 (MQ=255)
                                                                             cttcttTTAGGTAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGAt                                                                         <  2:1069590/150‑1 (MQ=255)
                                                                                cttTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGTTAAt                                                                      >  2:841294/1‑150 (MQ=255)
                                                                                cttTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAAt                                                                      >  2:308670/1‑150 (MQ=255)
                                                                                cttTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAAt                                                                      >  2:1434471/1‑150 (MQ=255)
                                                                                    tAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGt                                                                  <  2:389600/150‑1 (MQ=255)
                                                                                    tAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGt                                                                  <  2:626960/150‑1 (MQ=255)
                                                                                     aGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGtt                                                                 <  2:1729552/150‑1 (MQ=255)
                                                                                      gggAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTtc                                                                <  2:1121205/150‑1 (MQ=255)
                                                                                      gggAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTtc                                                                <  2:615263/150‑1 (MQ=255)
                                                                                       ggAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTtct                                                               >  1:931748/1‑150 (MQ=255)
                                                                                           aaCAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATACAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTGTCTaa                                                           >  2:1472024/1‑150 (MQ=255)
                                                                                           aaCAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTaa                                                           <  1:719111/150‑1 (MQ=255)
                                                                                             cAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAAtt                                                         >  2:1454892/1‑149 (MQ=255)
                                                                                              aGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGt                                                        <  2:719428/150‑1 (MQ=255)
                                                                                               ggAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACCGTTTTTTATCGACCAGATAATCTGTTCTCTAATGtt                                                       <  2:1483795/150‑1 (MQ=255)
                                                                                                 agaACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTaa                                                     <  1:1611588/150‑1 (MQ=255)
                                                                                                      cgccgAACTATCTCTATTTATAATTTAAAGTTATATCTGCCCCGCTAAAACGGGGCATATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTccc                                                <  2:761421/150‑1 (MQ=255)
                                                                                                              tATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCt                                        >  1:743120/1‑150 (MQ=255)
                                                                                                              tATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCACTAACCt                                        >  1:301400/1‑150 (MQ=255)
                                                                                                                tatctATATATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTAATGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGt                                      <  2:68725/148‑1 (MQ=255)
                                                                                                                 ctctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGtt                                     <  1:1689475/150‑1 (MQ=255)
                                                                                                                  tctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTAATAAAGTTAATTTTGCAGTGAAGACATTTACTGTTTTTTATCGACCAGATAATCTTTTATCTAATGTTAACTCACCCTAACCTGttt                                    >  1:72576/1‑149 (MQ=255)
                                                                                                                   ctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGc                                   >  2:465663/1‑150 (MQ=255)
                                                                                                                   ctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGc                                   >  2:10877/1‑150 (MQ=255)
                                                                                                                   ctATTTATAATTTAATGGTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCATTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGc                                   <  2:1085723/150‑1 (MQ=255)
                                                                                                                       ttATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTATCGGTCATTTTGCCGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTa                               <  2:1104840/150‑1 (MQ=255)
                                                                                                                         ataATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGt                             <  2:587261/150‑1 (MQ=255)
                                                                                                                         ataATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCAGTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGt                             >  1:266414/1‑150 (MQ=255)
                                                                                                                          taATTTTATGTTTTATCTGGCCCGATAATAAGGGGCAGATAATATGTTTAGTTTATTTACGGTCATTTTGCAGTGTAGCGATTTACTGTTTTTTATCTACCAGATAATATGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGtt                            <  1:451864/150‑1 (MQ=255)
                                                                                                                          taATTTAATGTTATATCTGCCCTGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGGCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGtt                            <  1:346090/150‑1 (MQ=255)
                                                                                                                          taATTTAATGTTATATCTGCCACGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGtt                            <  1:887784/150‑1 (MQ=255)
                                                                                                                           aaTTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACTGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATGAGTTCTCTAATGTTAACTACCCCTAACCTGTTGCTTTAGTTa                           >  1:1127809/1‑150 (MQ=255)
                                                                                                                                  tGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCAttt                    >  1:1426684/1‑150 (MQ=255)
                                                                                                                                    ttATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTATTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCACCTAACCTGTTGCTTTAGTTATTCATTTcc                  <  2:145343/150‑1 (MQ=255)
                                                                                                                                    ttATATCTGCCCCGATAAAAAGGGTCAGATAATATGTTTAGTTTACTAACGTTAATTTTGCAGTGAAGCCATTTACTTTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTcc                  <  2:707625/150‑1 (MQ=255)
                                                                                                                                      ttatCTGCCCCGTTTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTg                >  1:966851/2‑150 (MQ=255)
                                                                                                                                      ttatCTGCCCCGTTTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTg                >  2:100827/2‑150 (MQ=255)
                                                                                                                                      atatCTGCCTCGATAAAAAGGTGCAGATAATATGTTTAGTTTACTATCGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTTTCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTTCTTTAGTTATTCATTTCCTg                <  1:275856/150‑1 (MQ=255)
                                                                                                                                      atatCTGCCCCGATAAAACGGGGAAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTg                <  1:1656612/150‑1 (MQ=255)
                                                                                                                                        atCTGCCCCGTTTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGtc              >  2:247967/1‑150 (MQ=255)
                                                                                                                                        atCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGtc              <  2:1331126/150‑1 (MQ=255)
                                                                                                                                        atCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTAATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATTATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGtc              >  2:928121/1‑150 (MQ=255)
                                                                                                                                         tCTGCCCCGTTTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTACTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGtct             >  2:1555799/1‑150 (MQ=255)
                                                                                                                                          cTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCACATAACCTGTGGCTTTAGTTATTCATTTCCTGtcta            >  2:835631/1‑149 (MQ=255)
                                                                                                                                             ccccGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACt         >  2:828951/1‑150 (MQ=255)
                                                                                                                                                 gATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGc     <  2:133277/150‑1 (MQ=255)
                                                                                                                                                  aTAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGcc    <  1:991184/150‑1 (MQ=255)
                                                                                                                                                    aaaaCGGGGAAGATAATATGTTTAGTTTACTATCGGTCATTTTGCATTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTTCTTTAGTTTTTCATTTCCTGTCTCACTTTGCCtt  <  1:1377244/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAAAAATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCCTT  >  NC_000913/2788525‑2788822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: