Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,800,517 T→A 7.6% intergenic (+42/‑206) ygaM → / → nrdH putative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,800,5170TA 7.6% 405.7 / 18.0 144intergenic (+42/‑206)ygaM/nrdHputative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein
Reads supporting (aligned to +/- strand):  ref base T (58/73);  new base A (6/5);  total (64/80)
Fisher's exact test for biased strand distribution p-value = 5.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.37e-01

Only 100 of 144 total aligned reads displayed.

TTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAG  >  NC_000913/2800369‑2800665
                                                                                                                                                    |                                                                                                                                                     
  agCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTc                                                                                                                                                    >  2:894625/2‑150 (MQ=255)
       cgcgGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGGACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCgg                                                                                                                                              <  2:1062056/150‑1 (MQ=255)
         cgGATTCTTTTGTTAGTGAAAGACCCTGGTGTAGCGATGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCTCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCAGAAGCCTTTCTTCgggt                                                                                                                                            >  1:858709/1‑149 (MQ=255)
           gATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGtc                                                                                                                                          <  2:1136802/150‑2 (MQ=255)
           gATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGAACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTACTGCAAAAATGATAATAAATACGCGGCTTTGACCCCGAAGACAGGCTTCGGGGtc                                                                                                                                          <  2:1186298/150‑2 (MQ=255)
             ttCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTTTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTc                                                                                                                                        >  1:1258275/1‑150 (MQ=255)
             ttCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAACTCGTAGTGCAAAAATGATAATAAATACGCGTCTTTTACACAGAAGCCTGTCTTCGAGGttac                                                                                                                                        >  1:142357/1‑148 (MQ=255)
              tCTTTTGTTCGTGATAGACCCTGGTGTAGCGTGGGTTCAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCt                                                                                                                                       <  1:1227445/150‑1 (MQ=255)
              tCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCt                                                                                                                                       >  1:188986/1‑150 (MQ=255)
               cTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCtt                                                                                                                                      >  1:920367/1‑150 (MQ=255)
               cTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGtcaaag                                                                                                                                      <  2:782084/150‑6 (MQ=255)
                ttttGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCttt                                                                                                                                     >  1:349453/1‑150 (MQ=255)
                  ttGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCttttt                                                                                                                                   >  2:377541/1‑150 (MQ=255)
                   tGTTCGTGAAATACCCTGGTGTAGCGTGGGTACAGCAGCTGCTGTAGGTTTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTg                                                                                                                                  <  2:1045007/150‑1 (MQ=255)
                   tGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTg                                                                                                                                  <  1:1494051/150‑1 (MQ=255)
                   tGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTg                                                                                                                                  >  2:897188/1‑150 (MQ=255)
                   tGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTg                                                                                                                                  <  2:1685491/150‑1 (MQ=255)
                    gTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTga                                                                                                                                 >  1:1045007/1‑149 (MQ=255)
                     ttCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGcc                                                                                                                                >  2:1641660/1‑150 (MQ=255)
                     ttCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGcc                                                                                                                                >  2:335147/1‑150 (MQ=255)
                     ttCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGcc                                                                                                                                <  2:550333/150‑1 (MQ=255)
                     ttCGTGAAAGACCCTGGTGTAGCGGGGGTACAGCAGCTGCGGTAGGTAGTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCTAAGCCTGTCTTCGGGGTTTCTTTTTGcc                                                                                                                                <  2:153505/150‑1 (MQ=255)
                       cGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCAAAGCCTGTCTTCGGGGTTTCTTTTTGCCTg                                                                                                                              >  1:565167/1‑150 (MQ=255)
                          gAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTg                                                                                                                           <  2:1114896/150‑1 (MQ=255)
                          gAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTg                                                                                                                           <  2:419813/150‑1 (MQ=255)
                          gAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTg                                                                                                                           >  1:186390/1‑150 (MQ=255)
                              gACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATc                                                                                                                       <  1:237980/150‑1 (MQ=255)
                                cccTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATcac                                                                                                                     >  2:373478/1‑150 (MQ=255)
                                   tGGTGTAGCGTGTGTACAGCAGCGGCGGTAGGTATTATTATCGGCGCTCTGTGAAGCATGCGCAAATCGTAGTGCAAAAATGATAATTAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaa                                                                                                                  <  1:241826/150‑1 (MQ=255)
                                        tAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATcc                                                                                                             >  1:1681020/1‑150 (MQ=255)
                                        tAGCGTGGGTACAGAAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTACGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATcc                                                                                                             <  1:70785/150‑1 (MQ=255)
                                         aGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAATCATGCGCAAATCGTAGTGCAATAATGATAATAAATACGCGTCTTTGACCCCGAATCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATccc                                                                                                            <  2:1717592/150‑1 (MQ=255)
                                          gCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATcccc                                                                                                           <  2:1119391/150‑1 (MQ=255)
                                                  aCAGCAGCTGCTGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCTCAAATCGTAGTGCAAAAATGATAATAAATACGCCTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCg                                                                                                   <  2:1614228/150‑1 (MQ=255)
                                                  aCAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCg                                                                                                   <  1:303106/150‑1 (MQ=255)
                                                     gcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa                                                                                                >  2:660603/1‑150 (MQ=255)
                                                        gcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCCCGc                                                                                             >  2:503510/1‑150 (MQ=255)
                                                          tGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTc                                                                                           <  2:1345234/150‑1 (MQ=255)
                                                            cGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCat                                                                                         <  2:920367/150‑1 (MQ=255)
                                                            cGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCACTCCCCCGTCACCCTCaa                                                                                         >  2:1058273/1‑149 (MQ=255)
                                                             ggtaggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCata                                                                                        >  1:1089428/1‑150 (MQ=255)
                                                             ggtaggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCata                                                                                        <  2:1311143/150‑1 (MQ=255)
                                                                aggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCACCCTACCCCGTCACGCGCATATcc                                                                                     >  2:284898/1‑150 (MQ=255)
                                                                  gtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAg                                                                                   <  2:462780/150‑1 (MQ=255)
                                                                   taTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAgg                                                                                  <  1:599684/150‑1 (MQ=255)
                                                                    ttttttATCGGCGCACTGTTAAGCATGCGCAAATCCTAGTGCAAAAATGATAATAAATACGCGTCTTAGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAggg                                                                                 <  2:450955/149‑1 (MQ=255)
                                                                    aTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAggg                                                                                 >  2:523350/1‑150 (MQ=255)
                                                                    aTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAggg                                                                                 <  2:1226001/150‑1 (MQ=255)
                                                                       tttATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTaa                                                                              <  2:33669/150‑1 (MQ=255)
                                                                       tttATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGGaa                                                                              >  1:1543479/1‑150 (MQ=255)
                                                                       tttATCGGCGCACTGTTAAGAATGCGCTAATCGTAGTTCTAAAATGATTATAATTACGCGTCTTTGTCCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTaa                                                                              <  2:1254103/150‑1 (MQ=255)
                                                                            cGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAATATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCg                                                                         <  2:262949/150‑1 (MQ=255)
                                                                            cGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCg                                                                         <  1:114984/150‑1 (MQ=255)
                                                                             ggCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGa                                                                        >  1:1246661/1‑150 (MQ=255)
                                                                              gcgcACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc                                                                       <  1:1313894/150‑1 (MQ=255)
                                                                               cgcACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAcc                                                                      <  2:1695075/150‑1 (MQ=255)
                                                                                 cACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGACTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCTACCAc                                                                    >  1:311698/1‑150 (MQ=255)
                                                                                     gTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAtt                                                                <  1:796114/150‑1 (MQ=255)
                                                                                     gTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCAAGGGTAATTTCGACCACTAtt                                                                >  2:160640/1‑150 (MQ=255)
                                                                                        aaGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc                                                             >  2:790037/1‑150 (MQ=255)
                                                                                           cATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCtat                                                          >  2:549815/1‑150 (MQ=255)
                                                                                           cATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCtat                                                          <  2:837184/150‑1 (MQ=255)
                                                                                              gcgcAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTCCCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCtatata                                                       >  2:118935/1‑150 (MQ=255)
                                                                                               cgcAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTatatat                                                      >  1:1589627/1‑150 (MQ=255)
                                                                                                      cGTAGTTCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTgg                                               <  1:466689/150‑1 (MQ=255)
                                                                                                      cGTAGTGCAAAATTGATAATAAATACGCGTCTTTGACCCCGAATCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATACCCCTACCCAGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTgg                                               <  2:858709/150‑1 (MQ=255)
                                                                                                       gTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGt                                              <  2:1246661/150‑1 (MQ=255)
                                                                                                       gTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGt                                              >  1:892800/1‑150 (MQ=255)
                                                                                                        tAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGtt                                             <  2:757626/150‑1 (MQ=255)
                                                                                                         aGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTg                                            <  1:849810/150‑1 (MQ=255)
                                                                                                         aGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGCCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTg                                            >  1:1230940/1‑150 (MQ=255)
                                                                                                          gTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTg                                            <  2:1481228/150‑1 (MQ=255)
                                                                                                           tGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGaa                                          >  1:350538/1‑150 (MQ=255)
                                                                                                           tGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGaa                                          <  1:894625/150‑1 (MQ=255)
                                                                                                             cataaaTGATAATAAATACTCGTCTTTGACCCCGAAGCCTTTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTTGTTGAATc                                        <  1:251104/147‑1 (MQ=255)
                                                                                                               aaaaTGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTCAATCACAAAAATCACCCTACCCCGACACGCTCATATCCAGGGTAATATCGACCACTATTTGCTATATATTGTGTGGTTGAATCtt                                      >  1:1248289/1‑150 (MQ=255)
                                                                                                                  aTGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCttttt                                   <  2:1221294/150‑1 (MQ=255)
                                                                                                                   tGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCtttttt                                  <  2:378187/150‑1 (MQ=255)
                                                                                                                   tGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCtttttt                                  <  2:627133/150‑1 (MQ=255)
                                                                                                                    tataataAATACGCGTCTTTGACCCCGAAGCCGGTCTTCGGGGGTTCTTTTTGCCTGGGGCATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTc                                 <  1:1042150/149‑1 (MQ=255)
                                                                                                                     ataataAATACGCGTCTTTGACCCCGAAGCCGGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCa                                <  2:1321051/150‑1 (MQ=255)
                                                                                                                      taataaATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCaa                               <  2:649103/150‑1 (MQ=255)
                                                                                                                        ataaataCGCGTCTTTGAACCCGAAGCCTGTCTTCGGGGTTTCTTTTTTCCTGGTGAATCACAAAAATCCCGCTACCCCGTCAGGCTCATATCCAGGTTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACt                             <  1:1172464/150‑1 (MQ=255)
                                                                                                                          aaataCGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGt                                              <  1:757854/131‑1 (MQ=255)
                                                                                                                              aCGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCt                       <  2:743314/150‑1 (MQ=255)
                                                                                                                               cgcgTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCCCTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTa                      >  2:1138034/1‑150 (MQ=255)
                                                                                                                               cgcgTCTTTGACCCCGAAGCCTGTCTTAGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCTCTATTTGCTATATATTGTGTGGTTTAATCTTTTTTCAACTACATCTa                      >  2:1335983/1‑150 (MQ=255)
                                                                                                                                  gTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTa                   <  2:948186/150‑1 (MQ=255)
                                                                                                                                  gTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTa                   <  1:335147/150‑1 (MQ=255)
                                                                                                                                    cTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTAta                 >  2:178695/1‑149 (MQ=255)
                                                                                                                                       tGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATctct              <  2:1409439/150‑1 (MQ=255)
                                                                                                                                       tGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATctct              <  1:404891/150‑1 (MQ=255)
                                                                                                                                       aaaCCCCGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATctct              >  1:1730157/3‑150 (MQ=255)
                                                                                                                                        aaCCCCGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAAGCCCCCTACCCCGTCCCGCTCATATCCAGGCTAATTTCGACCACTATTTGCTTTATATTGTGTTGTTGAATCTTTTTTCAAATACATCTAGTATCTCTg             >  2:1334844/2‑150 (MQ=255)
                                                                                                                                         aacccGAAGACAGGCTTCCGGGTTTCTATTTTCCTGGTGAAACACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTCTGCTTGAATGTTTTTTCACCTACATCTAGTATCTCTGt            >  2:699257/3‑150 (MQ=255)
                                                                                                                                         aCCCCGAAGCCTGTCATCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGt            <  1:910559/150‑1 (MQ=255)
                                                                                                                                         aCCCCGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGt            >  2:824590/1‑150 (MQ=255)
                                                                                                                                         aCCCCGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGt            >  1:504607/1‑150 (MQ=255)
                                                                                                                                          ccccGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCCCGCTCATAGCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTa           >  2:146403/1‑150 (MQ=255)
                                                                                                                                                  ccTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACaga   <  2:1220718/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAG  >  NC_000913/2800369‑2800665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: