Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,802,399 T→G 5.1% F351C (TTC→TGC)  nrdE → ribonucleoside‑diphosphate reductase 2, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,802,3990TG 5.1% 309.8 / 5.7 99F351C (TTC→TGC) nrdEribonucleoside‑diphosphate reductase 2, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (56/38);  new base G (3/2);  total (59/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

Only 100 of 106 total aligned reads displayed.

AGAGAATGCGCAGATGGCGCTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATG  >  NC_000913/2802250‑2802548
                                                                                                                                                     |                                                                                                                                                     
agagAATGCGCAGATGGCGCTGTTTTCGCCTTATGATGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACACATTCGCAAAAAATACCTCAACGCCCGTGATttctt                                                                                                                                                       <  2:562289/150‑1 (MQ=255)
agagAATGCGCAGATGGCGCTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATttctt                                                                                                                                                       <  1:951866/150‑1 (MQ=255)
  agaATGCGCAGATGGCGCTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTcc                                                                                                                                                     <  2:1202285/150‑1 (MQ=255)
  agaATGCGCAGATGGCGCTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTcc                                                                                                                                                     <  2:874148/150‑1 (MQ=255)
   gaATGCGCAGATGGCGCTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCTCAAAAAATACCTCAACGCCCGTGATTTCTTCCa                                                                                                                                                    <  2:1207851/150‑1 (MQ=255)
     aTGCGCAGATGGCGCTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGc                                                                                                                                                  <  1:1283352/150‑1 (MQ=255)
      tGCGCAGATGGCGCTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCg                                                                                                                                                 >  1:989093/1‑150 (MQ=255)
        cgcAGATGGCGCTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGAc                                                                                                                                               >  1:1488494/1‑150 (MQ=255)
             aTGGCGCTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCa                                                                                                                                          <  2:145778/150‑1 (MQ=255)
                gcgcTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGaa                                                                                                                                       >  2:780850/1‑150 (MQ=255)
                  gcTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTAGCCGATGTGGCCATCAGCCAACACTATGACGATCTGGTTGACGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAAt                                                                                                                                     >  2:205460/1‑150 (MQ=255)
                   cTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATc                                                                                                                                    >  1:1047025/1‑150 (MQ=255)
                    tGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGACATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACATCAACGCCCGTGATTTCTTCCAGCGAATGGCAGAAATcc                                                                                                                                   >  1:1306723/1‑150 (MQ=255)
                    tGTTTTAGGCTTCTGACGTAGAGAGAGTTTATGGGAAGACGTTTGCCGATGGGGCCATCAGCCAACACTATGACGAACTGGGTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATcc                                                                                                                                   <  2:312065/150‑1 (MQ=255)
                        ttCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTGCCAGTCGCTGGAAGAAATcacggg                                                                                                                               <  1:327677/150‑6 (MQ=255)
                         tCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGttt                                                                                                                              <  1:523428/150‑1 (MQ=255)
                          cGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTg                                                                                                                             <  1:547919/150‑1 (MQ=255)
                           gCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCTATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGa                                                                                                                            <  1:883250/150‑1 (MQ=255)
                              ttATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGACCTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCAAGTTTGAGTc                                                                                                                         >  1:1061851/1‑150 (MQ=255)
                              ttATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATACAGTTTGAGTc                                                                                                                         >  1:1056498/1‑150 (MQ=255)
                               tATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTcc                                                                                                                        >  1:442086/1‑150 (MQ=255)
                                  gACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGc                                                                                                                     <  1:605884/150‑1 (MQ=255)
                                   aCGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCt                                                                                                                    <  2:1633742/150‑1 (MQ=255)
                                    cGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTa                                                                                                                   >  1:141143/1‑150 (MQ=255)
                                    cGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTa                                                                                                                   >  1:1373111/1‑150 (MQ=255)
                                     gTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTAt                                                                                                                  >  1:905583/1‑150 (MQ=255)
                                         agCGAGTTTTTGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCGGTGATTTCTTCCAGCGTCTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCt                                                                                                              <  2:267122/150‑1 (MQ=255)
                                         agCGAGTTTATGGCAAGACGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTAGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCt                                                                                                              <  2:1218165/150‑1 (MQ=255)
                                          gCGAGTTTATGGCTAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTa                                                                                                             <  1:193602/150‑1 (MQ=255)
                                              gTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTAcatc                                                                                                         >  1:1213368/1‑150 (MQ=255)
                                                ttATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTAcatcat                                                                                                       >  1:861874/1‑150 (MQ=255)
                                                ttATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTAcatcat                                                                                                       >  2:1027817/1‑150 (MQ=255)
                                                  aTGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGt                                                                                                     <  1:1228675/150‑1 (MQ=255)
                                                     gCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCACCGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATg                                                                                                  >  1:931095/1‑150 (MQ=255)
                                                      cAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGa                                                                                                 >  2:339414/1‑150 (MQ=255)
                                                      cAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGa                                                                                                 >  2:1587193/1‑150 (MQ=255)
                                                      cAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCAGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGa                                                                                                 >  1:1341391/1‑150 (MQ=255)
                                                       aaGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGaa                                                                                                <  1:1324850/150‑1 (MQ=255)
                                                       aaGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGaa                                                                                                <  1:12494/150‑1 (MQ=255)
                                                       aaGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGaa                                                                                                >  1:1571297/1‑150 (MQ=255)
                                                        aGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAg                                                                                               <  2:323064/150‑1 (MQ=255)
                                                            gTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGacac                                                                                           >  2:1692914/1‑150 (MQ=255)
                                                             tttGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACg                                                                                          <  1:1478395/150‑1 (MQ=255)
                                                                gCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTa                                                                                       >  1:1391643/1‑150 (MQ=255)
                                                                   gATGTGGCAATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATAACTCAACGCCCGTGATTTCTTCATGCGACTGGCAGAAATACATTTTGAGTCCGGGTATCACTTCATCATTTATTAAGAAACTGTaaag                                                                                    >  2:149541/1‑149 (MQ=255)
                                                                       tgGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTg                                                                                >  1:1442304/1‑150 (MQ=255)
                                                                          ccATCAGCCAACACTATGACGAACTGGGTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTTTACCGTgtct                                                                             <  1:153329/150‑4 (MQ=255)
                                                                             tCAGCCAACAATATGACGACCTGGTTGCCGATGAACGCATGCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTGTGAGTCCGGCTCTCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAAcc                                                                          <  1:867845/150‑1 (MQ=255)
                                                                               aGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCt                                                                        >  1:437522/1‑150 (MQ=255)
                                                                               aGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCt                                                                        <  1:1730309/150‑1 (MQ=255)
                                                                                gCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTa                                                                       >  2:1591497/1‑150 (MQ=255)
                                                                                gCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTa                                                                       >  1:1387978/1‑150 (MQ=255)
                                                                                       cTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCgg                                                                >  1:1176682/1‑150 (MQ=255)
                                                                                        tATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGGCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCggg                                                               <  2:1047025/150‑1 (MQ=255)
                                                                                         aTGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGgc                                                              >  2:1323792/1‑150 (MQ=255)
                                                                                          tGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGgcg                                                             <  1:1692914/150‑1 (MQ=255)
                                                                                           taCGAACTGGTTGCCGATGAACGCATTCGCAAAAAATCCCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCAACATCAAGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGgcgc                                                            <  2:1523215/149‑1 (MQ=255)
                                                                                            aCGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCa                                                           >  2:674985/1‑150 (MQ=255)
                                                                                                       gCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGt                                                >  1:3885/1‑150 (MQ=255)
                                                                                                       gCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGt                                                <  1:1418959/150‑1 (MQ=255)
                                                                                                         cGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTaa                                              >  1:1525845/1‑150 (MQ=255)
                                                                                                         cGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTaa                                              >  1:650871/1‑150 (MQ=255)
                                                                                                          gATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTaaa                                             >  1:412790/1‑149 (MQ=255)
                                                                                                              aaCGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGACGGGCGCATAAATATGAGTAATctct                                         >  2:105193/1‑150 (MQ=255)
                                                                                                               aCGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTACCCCTATCGCCGGGCGCATAAATATGAGTAATCTCTg                                        >  2:745235/1‑150 (MQ=255)
                                                                                                               aCGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTg                                        >  2:188833/1‑150 (MQ=255)
                                                                                                                cGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGc                                       <  1:859751/150‑1 (MQ=255)
                                                                                                                 gCATTCGCAAAAAATACCTCAACGCCCTTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCt                                      <  1:636984/150‑1 (MQ=255)
                                                                                                                  cATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCTACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTc                                     >  2:1222234/1‑150 (MQ=255)
                                                                                                                  cATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTc                                     >  1:840514/1‑150 (MQ=255)
                                                                                                                  cATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTc                                     >  1:523532/1‑150 (MQ=255)
                                                                                                                   aTTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCACTACATCATGTATGAAGACACGGTAAACCTTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCtaa                                    >  1:1418865/1‑148 (MQ=255)
                                                                                                                    ttCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAg                                   >  1:1358796/1‑150 (MQ=255)
                                                                                                                      cGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGaa                                 >  1:1370084/1‑150 (MQ=255)
                                                                                                                       gCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGaaa                                >  1:555721/1‑150 (MQ=255)
                                                                                                                          aaaaaTACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAAttt                             >  1:181155/1‑150 (MQ=255)
                                                                                                                           aaaaTACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAAtttt                            <  1:294913/150‑1 (MQ=255)
                                                                                                                             aaTACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGTGTAATCTCTGCTCAGAAATTTTGc                          <  2:1052634/150‑1 (MQ=255)
                                                                                                                               tACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAg                        <  1:1528370/150‑1 (MQ=255)
                                                                                                                               tACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGAAACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTACTCTCTGCTCAGAAATTTTGCAg                        >  1:1028338/1‑150 (MQ=255)
                                                                                                                                aCCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAgg                       <  1:1587193/150‑1 (MQ=255)
                                                                                                                                aCCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAgg                       <  1:339414/150‑1 (MQ=255)
                                                                                                                                  cTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATTAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGtt                     >  2:351364/1‑150 (MQ=255)
                                                                                                                                  cTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGtt                     >  1:914036/1‑150 (MQ=255)
                                                                                                                                   tacaCGCCCGTTATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCTTAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTa                    <  2:254490/147‑1 (MQ=255)
                                                                                                                                    cAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGATTCCGGCTATCCCTACAGCATGTATGCAGCCACGGTAAACCGTGCTCCCCCGCTCGCCGGGCGCAGAAATCGGAGGACGCGCTGCTCAGAAAGGTTGCAGGTGaa                   >  2:836258/1‑150 (MQ=255)
                                                                                                                                    cAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTaa                   >  2:1209554/1‑150 (MQ=255)
                                                                                                                                    cAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTaa                   >  2:416537/1‑150 (MQ=255)
                                                                                                                                     aaCGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAAc                  <  2:460596/150‑1 (MQ=255)
                                                                                                                                     aaCGCCCGTGATTTATTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAAc                  <  1:209999/150‑1 (MQ=255)
                                                                                                                                       cGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAg                <  2:442086/150‑1 (MQ=255)
                                                                                                                                          ccGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATCAAGACACGGTAAACCGTTCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAgcgc             >  2:640991/1‑150 (MQ=255)
                                                                                                                                          actggATTTCTGCCAGTCGCTGGAAGATATCCAGTTTGAGTCCGGCTATCACTACATCATGTATGAAGACACGGTATACAGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTAAGAAATTTTGCAGGTTATCAgcgc             >  1:53189/5‑150 (MQ=255)
                                                                                                                                          actggATTTCTGCCAGTCGCTGGAAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAgcgc             >  1:313432/5‑150 (MQ=255)
                                                                                                                                                tttCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCaga       >  2:963263/1‑150 (MQ=255)
                                                                                                                                                 ttcttcCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCagag      <  2:722142/150‑1 (MQ=255)
                                                                                                                                                 ttcttcCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCagag      <  2:1387978/150‑1 (MQ=255)
                                                                                                                                                   cttcCAGCGACTGGGAGAAATCCAGGTGGAGTCCGGCTATCCCTACATCATGTATGCAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTa    <  2:1476192/150‑1 (MQ=255)
                                                                                                                                                   cttcCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTa    >  2:686396/1‑150 (MQ=255)
                                                                                                                                                     tcCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATTAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGATAACAGCGCCTCAGAGTATg  >  1:1176925/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGAGAATGCGCAGATGGCGCTGTTTTCGCCTTATGACGTAGAGCGAGTTTATGGCAAGCCGTTTGCCGATGTGGCCATCAGCCAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATG  >  NC_000913/2802250‑2802548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: