Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,802,654 A→T 6.5% E436V (GAG→GTG) ‡ nrdE → ribonucleoside‑diphosphate reductase 2, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,802,6540AT 6.5% 306.1 / 8.9 107E436V (GAG→GTG) ‡nrdEribonucleoside‑diphosphate reductase 2, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (46/54);  new base T (4/3);  total (50/57)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

Only 100 of 111 total aligned reads displayed.

CTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTATG  >  NC_000913/2802511‑2802803
                                                                                                                                                |                                                                                                                                                     
cTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGc                                                                                                                                                 >  2:1660732/1‑150 (MQ=255)
 tCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGcc                                                                                                                                                <  2:437522/150‑1 (MQ=255)
   aGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGACCGCACGGTAGAGACTGCCGt                                                                                                                                              >  2:1452966/1‑150 (MQ=255)
   aGAAATATTGCAGGTTAACAGCGCCTCAGAGTATGCCGAGAATCTCGACTATACCCGCACAGGCCCTGATATTTCCTGCAATTTAGGTTCGTTGAAAATTGAGCACACCATAGATTCCCACGATTTAGCCCGCACGGTAGAGACTGCCga                                                                                                                                              >  2:1109121/1‑149 (MQ=255)
   aGAAAATTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTAAACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGt                                                                                                                                              <  2:53189/150‑1 (MQ=255)
    gAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTg                                                                                                                                             <  2:1389673/150‑1 (MQ=255)
    gAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATATAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTg                                                                                                                                             >  1:572946/1‑150 (MQ=255)
     aaaTTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTgc                                                                                                                                            >  2:1017716/1‑150 (MQ=255)
     aaaTTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGCAGTCTCTACCGTgc                                                                                                                                            <  1:674985/150‑1 (MQ=255)
       aTTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTgcgc                                                                                                                                          >  1:656554/1‑150 (MQ=255)
         tttGAAGGTTAACAGCGCCTCATCGTAGGCCGAGAATCTCGCCTATACCCGCACACGCCCTGATATTTCCTGCACGTTAGGGGCGGTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCgg                                                                                                                                        <  1:836258/150‑1 (MQ=255)
           tGCAGGTTAACAGCGCCTCAGATTAAGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGGTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGtt                                                                                                                                      <  2:1442304/150‑1 (MQ=255)
           tGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGtt                                                                                                                                      <  2:283794/150‑1 (MQ=255)
             cAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGCCTGCCGTGCGCGGtttt                                                                                                                                    >  1:765983/1‑149 (MQ=255)
             cAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTa                                                                                                                                    >  2:1643615/1‑150 (MQ=255)
              aGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTaa                                                                                                                                   <  1:1169096/150‑1 (MQ=255)
               ggTTACCAGCGCCTCTTAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGTTATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAAc                                                                                                                                  <  1:543614/150‑1 (MQ=255)
               ggTTAACATCGCCTCATAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCCCACGGTAGAGACTGCCGTGCGCGGTTTAAc                                                                                                                                  <  1:205460/150‑1 (MQ=255)
               ggTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAAc                                                                                                                                  <  2:1370084/150‑1 (MQ=255)
               ggTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGAAATATAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAAc                                                                                                                                  >  2:1058466/1‑150 (MQ=255)
                gTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCTCGCACGGTAGAGACTGCCGTGCGCGGTTTAACg                                                                                                                                 <  2:354959/150‑1 (MQ=255)
                 ttAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACgg                                                                                                                                <  2:1207983/150‑1 (MQ=255)
                  tAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACCCCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGc                                                                                                                               >  2:1207397/1‑150 (MQ=255)
                   aaCAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCAGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCa                                                                                                                              >  2:609103/1‑150 (MQ=255)
                      aGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTa                                                                                                                           <  2:181155/150‑1 (MQ=255)
                       gcgcCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTAt                                                                                                                          >  1:1029596/1‑150 (MQ=255)
                        cgcCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATc                                                                                                                         <  1:416537/150‑1 (MQ=255)
                        cgcCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATc                                                                                                                         >  2:124961/1‑150 (MQ=255)
                          catCAGAGTATGACGTGATTCTCGACTATAGCCGCACAGGCCATGATATTTCCTGCAATTTTGGTTCGTTGAATATTGAGCACACGATGGATTCCGCCGATTTTGCCCGCACGGTTGAGACTGCCGTGCGCGGTTTAACGGGAGTATCAg                                                                                                                       <  2:442863/148‑1 (MQ=255)
                            tCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGat                                                                                                                     <  1:1723934/150‑1 (MQ=255)
                            tCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGat                                                                                                                     <  1:1323792/150‑1 (MQ=255)
                              agagTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTTCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGatat                                                                                                                   >  2:1097982/1‑150 (MQ=255)
                                 gTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAg                                                                                                                <  1:1214650/150‑1 (MQ=255)
                                  tATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGt                                                                                                               <  1:754643/150‑1 (MQ=255)
                                   aTGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGta                                                                                                              >  2:612348/1‑149 (MQ=255)
                                   aTGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTc                                                                                                              <  2:436363/150‑1 (MQ=255)
                                    tGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCCCGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCa                                                                                                             <  1:351364/150‑1 (MQ=255)
                                    tGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCa                                                                                                             <  1:686396/150‑1 (MQ=255)
                                       cGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCatat                                                                                                          >  2:2859/1‑150 (MQ=255)
                                           aaTCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGc                                                                                                      >  1:1638057/1‑150 (MQ=255)
                                            aTCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCa                                                                                                     >  1:808590/1‑150 (MQ=255)
                                               tcGTCTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTGGTTGAATATTTCGCACACCATGGATTCCCCCGATTTTGCGCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCg                                                                                                  <  2:1176925/150‑1 (MQ=255)
                                                cGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGt                                                                                                 >  1:1100645/1‑150 (MQ=255)
                                                cGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATCGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCACATATGTGTCATATCAGCAGCGt                                                                                                 >  1:350332/1‑150 (MQ=255)
                                                  aCTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGc                                                                                               <  2:737999/150‑1 (MQ=255)
                                                  aCTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGc                                                                                               >  1:785655/1‑150 (MQ=255)
                                                   cTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGcc                                                                                              >  1:1435862/1‑150 (MQ=255)
                                                    aataCCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCg                                                                                             <  2:313432/149‑1 (MQ=255)
                                                     ataCCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGt                                                                                            <  1:1591497/150‑1 (MQ=255)
                                                      taCCCGCACAGTCCATGATATTTCCTGCAATTTAGGTTCTTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTc                                                                                           <  1:1287982/150‑1 (MQ=255)
                                                      taCCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTc                                                                                           <  1:1710161/150‑1 (MQ=255)
                                                        cccGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTcc                                                                                          <  2:470720/150‑1 (MQ=255)
                                                          cGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATc                                                                                       >  2:430780/1‑150 (MQ=255)
                                                              caGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAg                                                                                   >  2:693270/1‑150 (MQ=255)
                                                                ggCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGcc                                                                                 <  2:1638057/150‑1 (MQ=255)
                                                                ggCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGcc                                                                                 <  1:1643615/150‑1 (MQ=255)
                                                                    aTGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGaa                                                                             >  2:1269699/1‑150 (MQ=255)
                                                                         atTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATgcc                                                                        <  2:1126414/150‑1 (MQ=255)
                                                                              cTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTc                                                                   >  1:368098/1‑150 (MQ=255)
                                                                                gCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGc                                                                 >  1:1309356/1‑150 (MQ=255)
                                                                                 cAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTCGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCa                                                                <  1:509039/150‑1 (MQ=255)
                                                                                     ttAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGcc                                                            <  1:430254/150‑1 (MQ=255)
                                                                                     ttAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGcc                                                            >  1:440623/1‑150 (MQ=255)
                                                                                     ttAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGcc                                                            <  1:745235/150‑1 (MQ=255)
                                                                                     ttAGGTGCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGcc                                                            <  1:188833/150‑1 (MQ=255)
                                                                                          ttCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCAATCGAAGCCGGAAATGCCGCCTCGCACGCCATCgg                                                       >  1:141701/1‑150 (MQ=255)
                                                                                            cGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGAc                                                     <  2:1039618/150‑1 (MQ=255)
                                                                                             gTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCCTCGGACt                                                    >  2:752042/1‑150 (MQ=255)
                                                                                             gTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACt                                                    >  1:1147811/1‑150 (MQ=255)
                                                                                             gTTGAATATTGCGCACACCATGGATTCACCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGAcg                                                    >  1:302227/1‑149 (MQ=255)
                                                                                               tGAATATTGCGCACACCATTGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTgg                                                  <  1:149541/150‑1 (MQ=255)
                                                                                               tGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGAGATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTgg                                                  >  2:1178330/1‑150 (MQ=255)
                                                                                                 aaTATTGAGCACACCATGGATTCCCCCGCTTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTgggg                                                <  2:199728/150‑1 (MQ=255)
                                                                                                      tGCGCACACCATTGATTCCCGCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTCACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCGCGCACGCCATCGGACTGGGGCAGAt                                           <  1:640991/150‑1 (MQ=255)
                                                                                                        cgcACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCACGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGa                                         <  1:817495/150‑1 (MQ=255)
                                                                                                          cacaCCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAAt                                       >  1:1003153/1‑150 (MQ=255)
                                                                                                           acacCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAAtt                                      <  1:1017716/150‑1 (MQ=255)
                                                                                                              ccATGGATTCCCCCGATTTTGCCCGCACCGCAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGGCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTac                                   <  1:799893/150‑1 (MQ=255)
                                                                                                               cATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTaca                                  <  2:1028338/150‑1 (MQ=255)
                                                                                                                 tGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACg                                <  1:405478/150‑1 (MQ=255)
                                                                                                                   gATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGc                              <  1:944277/150‑1 (MQ=255)
                                                                                                                        ccccGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCt                         >  1:1137486/1‑150 (MQ=255)
                                                                                                                        ccccGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCt                         >  2:748422/1‑150 (MQ=255)
                                                                                                                        ccccGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCt                         >  2:60031/1‑150 (MQ=255)
                                                                                                                         cccGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTg                        >  2:1678002/1‑150 (MQ=255)
                                                                                                                              ttttGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGcgcg                   >  1:922029/1‑150 (MQ=255)
                                                                                                                              ttttGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGcgcg                   <  1:1404630/150‑1 (MQ=255)
                                                                                                                                  accgcGCACGGCAGTCTCTACCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCgaga                >  1:1100189/5‑150 (MQ=255)
                                                                                                                                  accgcGCACGGCAGTCTCTACCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGACCATCGAAGCCGGACATGCCGCCTCGCACGCCATCGCACTGGGGCAGATGAATTTACACGGCTATCTGGAGCgaga                >  1:1560421/5‑150 (MQ=255)
                                                                                                                                   ccgcGCACGGCAGTCTCTACCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGCATTTAAACGGCTATCTGGCGCGAGaa               >  1:1119432/4‑150 (MQ=255)
                                                                                                                                   ccgcGCACGGCAGTCTCTACCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGaa               >  2:1443663/4‑150 (MQ=255)
                                                                                                                                    cccGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAg              >  2:1433603/1‑150 (MQ=255)
                                                                                                                                    cccGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAg              >  2:190465/1‑150 (MQ=255)
                                                                                                                                            gTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCTCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCt      <  2:572946/150‑1 (MQ=255)
                                                                                                                                            gTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCt      <  2:675802/150‑1 (MQ=255)
                                                                                                                                            gTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCt      <  1:646351/150‑1 (MQ=255)
                                                                                                                                              agagACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTa    <  2:1435862/150‑1 (MQ=255)
                                                                                                                                               gagaCTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTAt   <  1:1581165/150‑1 (MQ=255)
                                                                                                                                                agaCTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGTGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTATg  <  1:1058466/150‑1 (MQ=255)
                                                                                                                                                agaCTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTGATg  >  2:559457/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
CTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTATG  >  NC_000913/2802511‑2802803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: