Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 317,566 G→A 5.7% pseudogene (128/129 nt) ykgQ → pseudogene, putative dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913317,5660GA 5.7% 447.2 / 9.5 143pseudogene (128/129 nt)ykgQpseudogene, putative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (69/63);  new base A (3/5);  total (74/69)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.33e-01

Only 100 of 151 total aligned reads displayed.

GCAGATGCCCATCAAAAATCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGT  >  NC_000913/317419‑317715
                                                                                                                                                   |                                                                                                                                                     
gCAGATGCCCATCAAAAATCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACAtgattgat                                                                                                                                                     >  1:1538397/1‑150 (MQ=255)
gCAGATGCCCATCAAAAATCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACAtgattgat                                                                                                                                                     >  2:845223/1‑150 (MQ=255)
gCAGATGCCCATCAAAAATCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACAtgattgat                                                                                                                                                     >  2:1566659/1‑150 (MQ=255)
 cAGATGCCCATCAAAAATCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTTTCTTGCTGTAAAAATACATGATTGata                                                                                                                                                    >  1:147083/1‑150 (MQ=255)
   gATGCCCATCAAAAATCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATAtt                                                                                                                                                  <  1:532041/150‑1 (MQ=255)
       cccATCAAAAATCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAAta                                                                                                                                              >  2:509642/1‑149 (MQ=255)
       cccATCAAAAATCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATc                                                                                                                                              >  1:653447/1‑150 (MQ=255)
       cccATCAAAAATCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGATTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTCATATTAAta                                                                                                                                              >  1:460085/1‑149 (MQ=255)
          aTCAAAAATCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATTTTAATCATg                                                                                                                                           >  2:120703/1‑150 (MQ=255)
          aTCAAAAATCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATg                                                                                                                                           >  2:1696746/1‑150 (MQ=255)
            cAAAAATCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGTTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTAGGTAAAATACATGATTAATATCAATCATGTAtt                                                                                                                                       <  1:906967/150‑1 (MQ=38)
            cAAAAATCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTa                                                                                                                                         <  1:408366/150‑1 (MQ=255)
            cAAAAATCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTAATATCAATCATGTa                                                                                                                                         <  1:552157/150‑1 (MQ=255)
            cAAAAATCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGAGTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTa                                                                                                                                         >  1:772111/1‑150 (MQ=255)
             aaaaaTCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTAt                                                                                                                                        <  1:1695829/150‑1 (MQ=255)
                aaTCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTTATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTAtttt                                                                                                                                     <  2:95027/150‑1 (MQ=255)
                 aTCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCTGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTAATATCAATCATGTAttttt                                                                                                                                    <  1:820796/150‑2 (MQ=255)
                 aTCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTAGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTa                                                                                                                                    >  1:102287/1‑150 (MQ=255)
                    gATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCt                                                                                                                                 <  2:337652/150‑1 (MQ=255)
                    gATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGATCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCt                                                                                                                                 <  1:767326/150‑1 (MQ=255)
                      tttCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTaa                                                                                                                               >  1:1379392/1‑150 (MQ=255)
                        tctcACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAga                                                                                                                             >  1:813569/1‑150 (MQ=255)
                          tcACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAgacg                                                                                                                           <  2:653447/150‑1 (MQ=255)
                           cACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAgacga                                                                                                                          >  2:468347/1‑150 (MQ=255)
                             cTGACAGATAAAGAAATGGCACATATTAGTTCGATTGATTTGGGGTATGTTGGGGAGTCTGTAAAAAATTTTAATCCTGAATTTTTTCGTGTTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACa                                                                                                                        <  2:1369083/150‑1 (MQ=255)
                                  aGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAAc                                                                                                                   <  1:1558800/150‑1 (MQ=255)
                                  aGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAAc                                                                                                                   >  2:1267732/1‑150 (MQ=255)
                                   gATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAAcc                                                                                                                  >  2:307823/1‑150 (MQ=255)
                                      aaaGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCttt                                                                                                               <  2:1538397/150‑1 (MQ=255)
                                      aaaGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCttt                                                                                                               >  2:1094210/1‑150 (MQ=255)
                                       aaGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCtttt                                                                                                              <  2:881463/150‑1 (MQ=255)
                                       aaGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCtttt                                                                                                              <  2:223723/150‑1 (MQ=255)
                                          aaaTGGCAAAAATTAGTTCGCTTGATTTGGGTTATGTTTGGGAGTCGTTAAAACATTTTAATCATTAATTTTTTCTTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAAGCATGTATTTTACCTAAGACGACAAAAACCTTTTAAc                                                                                                           <  1:121393/150‑1 (MQ=255)
                                            aTGGCACAAATTAGTTAGATTTATTTGGTTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTTTTCGTTTTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATTTATTTTACCTAAGACGACAAAAACCTTTTAACAg                                                                                                         <  2:1490575/150‑1 (MQ=255)
                                              gtcacaAATTAGTTCGCTTGATTTGGGTTATTTTGGTGATTCGGTAAATCATTTTAATCCTGAATTTGTTCGTTGTTGTCTTGCTGTAATAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAggg                                                                                                       <  1:1148207/148‑1 (MQ=255)
                                              ggCTCAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAggg                                                                                                       <  1:1104118/150‑1 (MQ=255)
                                              ggCACAAATTAGTTCGCTTGATTTTGGTTATGTTGGGTATTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGCTTGTCTTGCGGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAggg                                                                                                       <  1:725362/150‑1 (MQ=255)
                                              ggCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTTGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAggg                                                                                                       <  1:94479/150‑1 (MQ=255)
                                              ggCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAggg                                                                                                       <  2:1620952/150‑1 (MQ=255)
                                              ggCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAggg                                                                                                       <  1:517205/150‑1 (MQ=255)
                                                cacaAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAg                                                                                                     >  1:323203/1‑150 (MQ=255)
                                                          ttCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGAt                                                                                           <  1:468347/150‑1 (MQ=255)
                                                          ttCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGAt                                                                                           <  2:1152518/150‑1 (MQ=255)
                                                            cGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCt                                                                                         <  2:18786/150‑1 (MQ=255)
                                                             gCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCtt                                                                                        >  2:1136340/1‑150 (MQ=255)
                                                              cTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTc                                                                                       >  2:1364704/1‑150 (MQ=255)
                                                              cTTGATTTGGGGTATGTTGGGGAGTCGGCAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGAGGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTc                                                                                       <  2:704795/150‑1 (MQ=255)
                                                                 gATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCAct                                                                                    >  2:1527322/1‑150 (MQ=255)
                                                                  aTTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCActc                                                                                   >  1:22922/1‑150 (MQ=255)
                                                                      gggTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTc                                                                               <  1:1267732/150‑1 (MQ=255)
                                                                        gTTATTTTTGGGAGTCGGTAATACATTTTAATCCTGTTTTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTTATCATGAATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCat                                                                             >  2:1097800/1‑150 (MQ=255)
                                                                        aggtatctGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCat                                                                             <  2:720861/143‑1 (MQ=255)
                                                                            tGTTGGGGAGTCTGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATAtct                                                                         <  1:692127/150‑1 (MQ=255)
                                                                              ttGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTcc                                                                       >  1:1238192/1‑150 (MQ=255)
                                                                              ttGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTcc                                                                       >  2:797164/1‑150 (MQ=255)
                                                                               tGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTATTCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCg                                                                      >  1:151064/1‑150 (MQ=255)
                                                                                ggggAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTAtt                                                                                                                                       <  1:39129/84‑1 (MQ=255)
                                                                                ggggAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTAtt                                                                                                                                       >  2:39129/1‑84 (MQ=255)
                                                                                  ggAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATATTCACTATGTCATATATCCGGTa                                                                   >  2:598028/1‑150 (MQ=255)
                                                                                       cGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAatatt                                                              >  2:1670842/1‑149 (MQ=255)
                                                                                         gTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGc                                                            <  1:1696746/150‑1 (MQ=255)
                                                                                           aaaaCATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGt                                                          >  2:213581/1‑150 (MQ=255)
                                                                                           aaaaCATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGt                                                          >  2:336969/1‑150 (MQ=255)
                                                                                           aaaaCATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGt                                                          >  2:181284/1‑150 (MQ=255)
                                                                                            aaaCATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTc                                                         >  1:568559/1‑150 (MQ=255)
                                                                                                aTTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGc                                                     >  2:165786/1‑150 (MQ=255)
                                                                                                aTTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGc                                                     <  2:161022/150‑1 (MQ=255)
                                                                                                     aaTCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTtcat                                                >  1:1498780/1‑150 (MQ=255)
                                                                                                        ccTGAATTTGTTGGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTtcatcatc                                            <  2:1284949/150‑1 (MQ=255)
                                                                                                          tGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCAGGTAATATGACGTAATGCTtcatcataa                                           >  1:164609/1‑148 (MQ=255)
                                                                                                           gAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGatat                                                                                               >  1:1584811/1‑97 (MQ=255)
                                                                                                           gAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGatat                                                                                               <  2:1584811/97‑1 (MQ=255)
                                                                                                            aaTTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGa                                         >  1:688327/1‑150 (MQ=255)
                                                                                                             aTTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGaa                                        >  2:1099932/1‑150 (MQ=255)
                                                                                                               tttttCGTGGTTGTCTTTCTGTAAAAATACATGATTGATATTAATCATGTACTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAg                                      <  2:147083/147‑1 (MQ=255)
                                                                                                               ttGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAg                                      <  2:1419540/150‑1 (MQ=255)
                                                                                                                tGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAgg                                     >  1:1612224/1‑150 (MQ=255)
                                                                                                                tGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTAATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAgg                                     >  1:1019437/1‑150 (MQ=255)
                                                                                                                 gTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAggg                                    <  2:332689/150‑1 (MQ=255)
                                                                                                                   tCGTGGTTGTCTTGCTGTAAAAATACATGAATGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCTGTAATATGGCGTCAGGCTTCATCATCAGAAAggggg                                  <  2:424616/150‑1 (MQ=255)
                                                                                                                    cGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGc                                 >  2:1067046/1‑150 (MQ=255)
                                                                                                                         ttGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGat                            <  1:1158751/150‑2 (MQ=255)
                                                                                                                            gcTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGcc                         <  1:1566659/149‑1 (MQ=255)
                                                                                                                              ttGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGCCTg                       <  2:1052845/150‑1 (MQ=255)
                                                                                                                              ttGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGCCTg                       <  2:1690205/150‑1 (MQ=255)
                                                                                                                               tGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCATCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGCCTGa                      <  2:845773/150‑1 (MQ=255)
                                                                                                                                gCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGCCTGaa                     >  1:1460820/1‑150 (MQ=255)
                                                                                                                                 cTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGCCTGaaa                    <  2:1331845/150‑1 (MQ=255)
                                                                                                                                  tGTAAAAATACATGATTGATAATAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGCCTGaaaa                   >  1:1292928/1‑150 (MQ=255)
                                                                                                                                   ttAAAAATACATGATTTATATTAATCATGTATTTTACCTAATACGACAAAAACCTTTTAACAGGGAGTGAAATTGAGCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGACAGGGGGCCTGACGCCTGaaaaa                  <  2:231866/149‑1 (MQ=255)
                                                                                                                                   gTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATATCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGTGGGCCTGACGCATGaaaaa                  >  2:1313649/1‑150 (MQ=255)
                                                                                                                                     aaaaaTACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGCCTGAAAAAGt                <  2:1430545/150‑1 (MQ=255)
                                                                                                                                      aaaaTACATGATTAATATCAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGCCTGAAAAAGTg               >  2:1253168/1‑150 (MQ=255)
                                                                                                                                       aaaTACATGATTGATATTAATCATGTATTTTAACTAAGACGACAAAAACCTTTTAACAGGGAGTGATAATGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGCCTGAAAAAGTGa              <  2:253060/150‑1 (MQ=255)
                                                                                                                                         aTACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACATGGAGTGATATTGATCTTCAATCTGTCATATATCCGGTAATATGGCGTCAGGCTTAATAAACAGAAAGGGGGCCTGACGCCTGAAAAAGTTaac            >  1:1095051/1‑150 (MQ=255)
                                                                                                                                           aCATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGCCTGAAAAAGTGaacaa          >  2:877059/1‑150 (MQ=255)
                                                                                                                                           aCATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGCCTGAAAAAGTGaacaa          >  1:359119/1‑150 (MQ=255)
                                                                                                                                            cATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGCCTGAAAAAGTGaacaac         >  1:254875/1‑150 (MQ=255)
                                                                                                                                             aTGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGCCTGAAAAAGTGAacaaca        <  2:793156/150‑1 (MQ=255)
                                                                                                                                                   gatATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAgt  >  1:1359800/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCAGATGCCCATCAAAAATCGATTTCTCACTGACAGATAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGT  >  NC_000913/317419‑317715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: