Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,802,659 G→A 6.2% A438T (GCC→ACC)  nrdE → ribonucleoside‑diphosphate reductase 2, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,802,6590GA 6.2% 353.4 / 8.7 112A438T (GCC→ACC) nrdEribonucleoside‑diphosphate reductase 2, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (48/57);  new base A (4/3);  total (52/60)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

Only 100 of 116 total aligned reads displayed.

CTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTATGGTTCG  >  NC_000913/2802511‑2802808
                                                                                                                                                     |                                                                                                                                                     
cTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGc                                                                                                                                                      >  2:1660732/1‑150 (MQ=255)
 tCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGcc                                                                                                                                                     <  2:437522/150‑1 (MQ=255)
   aGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGACCGCACGGTAGAGACTGCCGt                                                                                                                                                   >  2:1452966/1‑150 (MQ=255)
   aGAAATATTGCAGGTTAACAGCGCCTCAGAGTATGCCGAGAATCTCGACTATACCCGCACAGGCCCTGATATTTCCTGCAATTTAGGTTCGTTGAAAATTGAGCACACCATAGATTCCCACGATTTAGCCCGCACGGTAGAGACTGCCga                                                                                                                                                   >  2:1109121/1‑149 (MQ=255)
   aGAAAATTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTAAACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGt                                                                                                                                                   <  2:53189/150‑1 (MQ=255)
     aaaTTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTgc                                                                                                                                                 <  2:1213368/150‑1 (MQ=255)
     aaaTTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTgc                                                                                                                                                 >  2:1017716/1‑150 (MQ=255)
     aaaTTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGCAGTCTCTACCGTgc                                                                                                                                                 <  1:674985/150‑1 (MQ=255)
       aTTTTGCAGTTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGCAGTCTCTACCGTgcgg                                                                                                                                               <  1:320629/150‑2 (MQ=255)
       aTTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTgcgc                                                                                                                                               >  1:656554/1‑150 (MQ=255)
        ttttGCAGGTTAACAGCGCCTCAGAGTATGACGAGTATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGCAGTCTCTACCGTgcggg                                                                                                                                              <  1:768265/150‑3 (MQ=255)
         tttGAAGGTTAACAGCGCCTCATCGTAGGCCGAGAATCTCGCCTATACCCGCACACGCCCTGATATTTCCTGCACGTTAGGGGCGGTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCgg                                                                                                                                             <  1:836258/150‑1 (MQ=255)
           tGCAGGTTAACAGCGCCTCAGATTAAGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGGTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGtt                                                                                                                                           <  2:1442304/150‑1 (MQ=255)
           tGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGtt                                                                                                                                           <  2:283794/150‑1 (MQ=255)
             cAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGCCTGCCGTGCGCGGtttt                                                                                                                                         >  1:765983/1‑149 (MQ=255)
             cAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTa                                                                                                                                         >  2:1643615/1‑150 (MQ=255)
              aGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTaa                                                                                                                                        <  1:1169096/150‑1 (MQ=255)
               ggTTACCAGCGCCTCTTAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGTTATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAAc                                                                                                                                       <  1:543614/150‑1 (MQ=255)
               ggTTAACATCGCCTCATAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCCCACGGTAGAGACTGCCGTGCGCGGTTTAAc                                                                                                                                       <  1:205460/150‑1 (MQ=255)
               ggTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAAc                                                                                                                                       <  2:1370084/150‑1 (MQ=255)
               ggTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGAAATATAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAAc                                                                                                                                       >  2:1058466/1‑150 (MQ=255)
                gTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCTCGCACGGTAGAGACTGCCGTGCGCGGTTTAACg                                                                                                                                      <  2:354959/150‑1 (MQ=255)
                 ttAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACgg                                                                                                                                     <  2:1207983/150‑1 (MQ=255)
                      aGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTa                                                                                                                                <  2:181155/150‑1 (MQ=255)
                       gcgcCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTAt                                                                                                                               >  1:1029596/1‑150 (MQ=255)
                        cgcCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATc                                                                                                                              <  1:416537/150‑1 (MQ=255)
                        cgcCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATc                                                                                                                              >  2:124961/1‑150 (MQ=255)
                            tCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGat                                                                                                                          <  1:1723934/150‑1 (MQ=255)
                              agagTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTTCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGatat                                                                                                                        >  2:1097982/1‑150 (MQ=255)
                                 gTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAg                                                                                                                     <  1:1214650/150‑1 (MQ=255)
                                  tATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGt                                                                                                                    <  1:754643/150‑1 (MQ=255)
                                   aTGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGta                                                                                                                   >  2:612348/1‑149 (MQ=255)
                                   aTGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTc                                                                                                                   <  2:436363/150‑1 (MQ=255)
                                    tGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCCCGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCa                                                                                                                  <  1:351364/150‑1 (MQ=255)
                                    tGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCa                                                                                                                  <  1:686396/150‑1 (MQ=255)
                                       cGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGCATATTGCGCACACAATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACTGCAGTATCTGATATGAGTCatat                                                                                                               >  2:305047/1‑150 (MQ=255)
                                           aaTCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGc                                                                                                           >  1:1638057/1‑150 (MQ=255)
                                            aTCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCa                                                                                                          >  1:808590/1‑150 (MQ=255)
                                               tcGTCTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTGGTTGAATATTTCGCACACCATGGATTCCCCCGATTTTGCGCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCg                                                                                                       <  2:1176925/150‑1 (MQ=255)
                                                cGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGt                                                                                                      >  1:1100645/1‑150 (MQ=255)
                                                cGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATCGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCACATATGTGTCATATCAGCAGCGt                                                                                                      >  1:350332/1‑150 (MQ=255)
                                                  aCTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGc                                                                                                    <  2:737999/150‑1 (MQ=255)
                                                  aCTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGc                                                                                                    >  1:785655/1‑150 (MQ=255)
                                                   cTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGcc                                                                                                   >  1:1435862/1‑150 (MQ=255)
                                                    aataCCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCg                                                                                                  <  2:313432/149‑1 (MQ=255)
                                                     ataCCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGt                                                                                                 <  1:1591497/150‑1 (MQ=255)
                                                      taCCCGCACAGTCCATGATATTTCCTGCAATTTAGGTTCTTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTc                                                                                                <  1:1287982/150‑1 (MQ=255)
                                                      taCCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTc                                                                                                <  1:1710161/150‑1 (MQ=255)
                                                        cccGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTcc                                                                                               <  2:470720/150‑1 (MQ=255)
                                                          cGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATc                                                                                            >  2:430780/1‑150 (MQ=255)
                                                           gCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCg                                                                                           <  2:569720/150‑1 (MQ=255)
                                                           gCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCg                                                                                           <  2:1029596/150‑1 (MQ=255)
                                                              caGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAg                                                                                        >  2:693270/1‑150 (MQ=255)
                                                                ggCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGcc                                                                                      <  1:1643615/150‑1 (MQ=255)
                                                                    aTGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGaa                                                                                  >  2:1269699/1‑150 (MQ=255)
                                                                         atTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATgcc                                                                             <  2:1126414/150‑1 (MQ=255)
                                                                              cTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTc                                                                        >  1:368098/1‑150 (MQ=255)
                                                                                gCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGc                                                                      >  1:1309356/1‑150 (MQ=255)
                                                                                 cAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTCGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCa                                                                     <  1:509039/150‑1 (MQ=255)
                                                                                     ttAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGcc                                                                 >  1:440623/1‑150 (MQ=255)
                                                                                     ttAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGcc                                                                 <  1:430254/150‑1 (MQ=255)
                                                                                        ggTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGGTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATc                                                              <  1:1209554/150‑1 (MQ=255)
                                                                                          ttCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCAATCGAAGCCGGAAATGCCGCCTCGCACGCCATCgg                                                            >  1:141701/1‑150 (MQ=255)
                                                                                             gTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCCTCGGACt                                                         >  2:752042/1‑150 (MQ=255)
                                                                                             gTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACt                                                         >  1:1147811/1‑150 (MQ=255)
                                                                                             gTTGAATATTGCGCACACCATGGATTCACCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGAcg                                                         >  1:302227/1‑149 (MQ=255)
                                                                                               tGAATATTGCGCACACCATTGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTgg                                                       <  1:149541/150‑1 (MQ=255)
                                                                                               tGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGAGATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTgg                                                       >  2:1178330/1‑150 (MQ=255)
                                                                                                 aaTATTGAGCACACCATGGATTCCCCCGCTTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTgggg                                                     <  2:199728/150‑1 (MQ=255)
                                                                                                      tGCGCACACCATTGATTCCCGCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTCACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCGCGCACGCCATCGGACTGGGGCAGAt                                                <  1:640991/150‑1 (MQ=255)
                                                                                                          cacaCCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAAt                                            >  1:1003153/1‑150 (MQ=255)
                                                                                                           acacCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAAtt                                           <  1:1017716/150‑1 (MQ=255)
                                                                                                              ccATGGATTCCCCCGATTTTGCCCGCACCGCAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGGCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTac                                        <  1:799893/150‑1 (MQ=255)
                                                                                                               cATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTaca                                       >  2:1581576/1‑150 (MQ=255)
                                                                                                               cATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTaca                                       <  2:1028338/150‑1 (MQ=255)
                                                                                                                   gATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGc                                   <  1:944277/150‑1 (MQ=255)
                                                                                                                        ccccGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCt                              >  2:60031/1‑150 (MQ=255)
                                                                                                                        ccccGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCt                              >  2:748422/1‑150 (MQ=255)
                                                                                                                         cccGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTg                             >  2:1678002/1‑150 (MQ=255)
                                                                                                                         cccGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGACGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTg                             >  1:1563557/1‑150 (MQ=255)
                                                                                                                              ttttGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGcgcg                        >  1:922029/1‑150 (MQ=255)
                                                                                                                              ttttGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGcgcg                        <  1:1404630/150‑1 (MQ=255)
                                                                                                                                  accgcGCACGGCAGTCTCTACCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCgaga                     >  1:1100189/5‑150 (MQ=255)
                                                                                                                                  accgcGCACGGCAGTCTCTACCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGACCATCGAAGCCGGACATGCCGCCTCGCACGCCATCGCACTGGGGCAGATGAATTTACACGGCTATCTGGAGCgaga                     >  1:1560421/5‑150 (MQ=255)
                                                                                                                                   ccgcGCACGGCAGTCTCTACCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGCATTTAAACGGCTATCTGGCGCGAGaa                    >  1:1119432/4‑150 (MQ=255)
                                                                                                                                   ccgcGCACGGCAGTCTCTACCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGaa                    >  2:1443663/4‑150 (MQ=255)
                                                                                                                                    cccGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAg                   >  1:564284/1‑150 (MQ=255)
                                                                                                                                    cccGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAg                   >  2:190465/1‑150 (MQ=255)
                                                                                                                                    cccGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAg                   >  2:1433603/1‑150 (MQ=255)
                                                                                                                                            gTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCTCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCt           <  2:572946/150‑1 (MQ=255)
                                                                                                                                            gTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCt           <  2:675802/150‑1 (MQ=255)
                                                                                                                                            gTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCt           <  1:646351/150‑1 (MQ=255)
                                                                                                                                              agagACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTa         <  2:1435862/150‑1 (MQ=255)
                                                                                                                                                agaCTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGTGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTATg       <  1:1058466/150‑1 (MQ=255)
                                                                                                                                                agaCTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTGATg       >  2:559457/1‑150 (MQ=255)
                                                                                                                                                  aCTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTATGGt     >  1:1687972/1‑150 (MQ=255)
                                                                                                                                                     gCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTATGGTTCg  >  1:690322/1‑150 (MQ=255)
                                                                                                                                                     gCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTATGGTTCg  >  2:1321442/1‑150 (MQ=255)
                                                                                                                                                     gCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTAATGGTTCg  >  1:236687/1‑150 (MQ=255)
                                                                                                                                                     gCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGAGCCGTCCATCGAAGCCGGAAAAGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACAAAGCTATCTGGCGCGAGAAGGCAACGCTTATGGTTCg  >  1:824525/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTATGGTTCG  >  NC_000913/2802511‑2802808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: