Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,823,838 C→A 6.6% intergenic (‑69/+11) recA ← / ← pncC DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor/nicotinamide‑nucleotide amidohydrolase; NMN amidohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,823,8380CA 6.6% 313.2 / 7.3 106intergenic (‑69/+11)recA/pncCDNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor/nicotinamide‑nucleotide amidohydrolase; NMN amidohydrolase
Reads supporting (aligned to +/- strand):  ref base C (42/57);  new base A (4/3);  total (46/60)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 111 total aligned reads displayed.

ATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAG  >  NC_000913/2823690‑2823987
                                                                                                                                                    |                                                                                                                                                      
attgAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCAATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCACTTATACTGTATGCt                                                                                                                                                       <  2:1694452/147‑1 (MQ=255)
 tgGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTGTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGcgc                                                                                                                                                      >  2:118808/1‑148 (MQ=255)
 tgGAGCCTTTACCAAATTGTATCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTc                                                                                                                                                      <  2:385331/150‑1 (MQ=255)
     gCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATAc                                                                                                                                                  >  1:677611/1‑150 (MQ=255)
      ccTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATATCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACa                                                                                                                                                 >  2:398937/1‑150 (MQ=255)
        tttACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGt                                                                                                                                               <  1:391975/150‑1 (MQ=255)
         ttACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTa                                                                                                                                              <  1:384600/150‑1 (MQ=255)
            acAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATTTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCa                                                                                                                                           >  1:123338/2‑150 (MQ=255)
              aaaTTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATGCAGTATCAAg                                                                                                                                         >  1:28687/1‑150 (MQ=255)
              aaaTTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAg                                                                                                                                         <  1:1306358/150‑1 (MQ=255)
              aaaTTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGAGCATACAGTAtaatt                                                                                                                                         <  1:1423410/150‑5 (MQ=255)
               aaTTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGAGCATACAGTAtaattg                                                                                                                                        <  2:1115147/150‑6 (MQ=255)
               aaTTGTATCTCAATCTGGTCCATTGCTGCCGCCAACGCTTTCTGTTTGTTTTCTTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAgt                                                                                                                                        <  2:855735/150‑1 (MQ=255)
                 ttGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGAGCATACAGTAtaattgct                                                                                                                                      <  2:424084/150‑8 (MQ=255)
                  tgattCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCAATTTTACTCCAGTCATGCCGGCTAAAACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGtt                                                                                                                                     >  1:294863/4‑150 (MQ=255)
                  tGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGtt                                                                                                                                     <  1:1446702/150‑1 (MQ=255)
                   gTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGttt                                                                                                                                    <  2:800850/150‑1 (MQ=255)
                    tttCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGtttt                                                                                                                                   <  2:501995/150‑1 (MQ=255)
                      tctcAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCAATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGt                                                                                                                                 <  1:56271/150‑1 (MQ=255)
                        tcAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATTCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAg                                                                                                                               <  1:80164/150‑1 (MQ=255)
                             cTGGCCCAGAGCTGCCGACAACGCTTTCTGTTTGTTTTCGTAGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTAAACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAAtt                                                                                                                          <  1:185024/150‑1 (MQ=255)
                                gCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAAttgtt                                                                                                                       <  1:1221123/150‑1 (MQ=255)
                                   cAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGcc                                                                                                                    <  2:1239845/150‑1 (MQ=255)
                                   cAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTAGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGcc                                                                                                                    <  2:1733872/150‑1 (MQ=255)
                                         tgcCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAgg                                                                                                              <  1:803213/150‑1 (MQ=255)
                                          gccgccAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGt                                                                                                             >  2:1535624/1‑150 (MQ=255)
                                           ccgccAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTc                                                                                                            <  1:2037/150‑1 (MQ=255)
                                            cgccAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCt                                                                                                           <  2:813980/150‑1 (MQ=255)
                                             gccAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAATGTCTg                                                                                                          >  2:1120153/1‑150 (MQ=255)
                                                  cGCTTTCTGTTTGTTTTCGTCGATATCCATTTTTACTCCTGTCATGCAGTGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATg                                                                                                     <  1:716369/150‑1 (MQ=255)
                                                  cGCTTTCTGTTTGTGTTCGTCGATAGCCATTTTTACTCCTGTCAAGCCGTGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTCTGCTCATACAGTATCAAGAGTTTTGAAGAAATTGTTGCCACAAGGTCTGCAATg                                                                                                     <  2:324168/150‑1 (MQ=255)
                                                   gCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGTGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGc                                                                                                    <  1:1228982/150‑1 (MQ=255)
                                                     tttCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCAt                                                                                                  >  2:874227/1‑150 (MQ=255)
                                                     tttCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGCAGACCTTGTGGCAACAATTTCTACaaaacac                                                                                                  <  1:413187/150‑6 (MQ=255)
                                                       tCTGTTTGTTTTCGTTGATAGCCATTTTTACTCCTGTAATGCCGTGTAATACCGGATAGTCAATATTTTCTGTTGAAGCAATTATACTGTATGCTCATACATTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGTTCTGCAATGCATAc                                                                                                <  2:1306589/150‑1 (MQ=255)
                                                         tgtttgttTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGTTAGTCAATATGTTCTGTTGAAGCATTTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGc                                                                                              <  1:1323456/150‑1 (MQ=255)
                                                         tgtttgttTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGTAATGCATACGc                                                                                              <  1:794514/150‑1 (MQ=255)
                                                          gtttgtttTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCa                                                                                             >  1:1002082/1‑150 (MQ=255)
                                                           tttgtttTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAg                                                                                            <  1:476288/150‑1 (MQ=255)
                                                           tttgtttTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAg                                                                                            <  2:1111226/150‑1 (MQ=255)
                                                            ttgtttGCGTCGATAGCCATTTTACCTCCTGTCATGCCGTGTAATACCGGATAGTCAATATGTTCTTTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGt                                                                                           <  2:1147301/150‑1 (MQ=255)
                                                             tgtttTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTa                                                                                          >  2:1058388/1‑150 (MQ=255)
                                                                  tcgtcgATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTg                                                                                     <  2:1157490/150‑1 (MQ=255)
                                                                   cgtcgATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTga                                                                                    <  2:166297/150‑1 (MQ=255)
                                                                     tcgATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgacg                                                                                  <  2:552172/150‑1 (MQ=255)
                                                                      cgATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgacga                                                                                 >  1:331735/1‑150 (MQ=255)
                                                                          aGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCa                                                                             >  2:605427/1‑150 (MQ=255)
                                                                           gCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCAc                                                                            >  1:375984/1‑150 (MQ=255)
                                                                           gCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCAc                                                                            >  2:1267689/1‑150 (MQ=255)
                                                                            ccATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCAcc                                                                           <  2:1323767/150‑1 (MQ=255)
                                                                            ccATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCAcc                                                                           >  2:174076/1‑150 (MQ=255)
                                                                              aTTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGc                                                                         >  1:1561998/1‑150 (MQ=255)
                                                                                 tttACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATc                                                                      >  2:739718/1‑150 (MQ=255)
                                                                                    aCTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACg                                                                   >  2:1147902/1‑150 (MQ=255)
                                                                                            cATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATTCAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTcgccgc                                                           <  2:28687/150‑1 (MQ=255)
                                                                                            cATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTcgccgc                                                           >  2:1029445/1‑150 (MQ=255)
                                                                                              tGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTg                                                         <  1:1726537/150‑1 (MQ=255)
                                                                                               gCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGa                                                        <  1:1640234/150‑1 (MQ=255)
                                                                                                 cGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAg                                                      <  1:461456/150‑1 (MQ=255)
                                                                                                  gggTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGATGAAGc                                                     >  1:661729/1‑150 (MQ=255)
                                                                                                   ggTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACAGCATCACGGTCGCCGCTGAAGCa                                                    >  2:279573/1‑150 (MQ=255)
                                                                                                       aTACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTcc                                               <  1:1685970/150‑1 (MQ=255)
                                                                                                       aTACCGGATAGTCAATATGTTATGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTcc                                               <  2:677611/150‑1 (MQ=255)
                                                                                                            ggATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTCCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCccgccg                                          >  1:826248/1‑150 (MQ=255)
                                                                                                                aGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTa                                      <  1:1067987/150‑1 (MQ=255)
                                                                                                                 gTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGGCGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTaa                                     >  1:82269/1‑150 (MQ=255)
                                                                                                                 gTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACTCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTaa                                     >  1:63903/1‑150 (MQ=255)
                                                                                                                 gTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTaa                                     >  2:1245606/1‑150 (MQ=255)
                                                                                                                  tCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAAt                                    >  1:746063/1‑150 (MQ=255)
                                                                                                                   cAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATg                                   >  1:48198/1‑150 (MQ=255)
                                                                                                                   cAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATg                                   >  2:1679040/1‑150 (MQ=255)
                                                                                                                          ttCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGGTTTGTAGAAATTGTGGCCACAAGGGCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAGGCATTACCCGGCGGGAATGcttcagc                            <  1:440126/150‑7 (MQ=255)
                                                                                                                            cTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACcg                          <  1:1680851/150‑1 (MQ=255)
                                                                                                                             tGTTGAAGCAATTATACTGTATTCTCATACAGTATCAAGTGTTTTTTAGAAATTGTTGCCACAAGGTCTGCAATGCATAAGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACcgc                         <  2:146647/150‑1 (MQ=255)
                                                                                                                             tGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACcgc                         >  1:271579/1‑150 (MQ=255)
                                                                                                                              gTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACcgcg                        >  1:1471230/1‑150 (MQ=255)
                                                                                                                              gTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACcgcg                        >  1:1232271/1‑150 (MQ=255)
                                                                                                                                tGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCggg                      <  1:1486035/150‑1 (MQ=255)
                                                                                                                                tGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCggg                      <  1:1492859/150‑1 (MQ=255)
                                                                                                                                 gAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGc                     <  1:1447053/150‑1 (MQ=255)
                                                                                                                                  aaGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCa                    <  1:1679040/150‑1 (MQ=255)
                                                                                                                                       aTTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGc               <  1:974181/150‑1 (MQ=255)
                                                                                                                                        ttATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGc                     >  1:472342/1‑143 (MQ=255)
                                                                                                                                        ttATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGc                     <  2:472342/143‑1 (MQ=255)
                                                                                                                                          ataCTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCaaa            >  2:644885/1‑150 (MQ=255)
                                                                                                                                          ataCTGTATGAGCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCaaa            >  1:1474306/1‑150 (MQ=255)
                                                                                                                                           taCTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCaaaa           >  2:870510/1‑150 (MQ=255)
                                                                                                                                           taCTGTATGAGCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCaaaa           >  1:1270180/1‑150 (MQ=255)
                                                                                                                                            aCTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAg          >  2:365659/1‑150 (MQ=255)
                                                                                                                                             cTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGc         <  2:1346761/150‑1 (MQ=255)
                                                                                                                                              tGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCa        >  1:1161203/1‑150 (MQ=255)
                                                                                                                                               gTATGAGCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCaa       >  2:1223051/1‑150 (MQ=255)
                                                                                                                                                tATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCaaa      <  1:319184/150‑1 (MQ=255)
                                                                                                                                                 aTGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAAc     <  1:1434093/150‑1 (MQ=255)
                                                                                                                                                 aTGCTCATACAGTATCAAGAGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCAGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAAc     <  2:123338/150‑1 (MQ=255)
                                                                                                                                                   gCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCTCAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGCTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAACCCa   >  2:1648246/1‑150 (MQ=255)
                                                                                                                                                   gCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCa   <  1:398937/150‑1 (MQ=255)
                                                                                                                                                    cTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATg                                   >  1:88521/1‑117 (MQ=255)
                                                                                                                                                    cTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAg  >  1:245421/1‑150 (MQ=255)
                                                                                                                                                    cTCATACAGTATCAAGTGTTTTGTAGAAATTCTTGCCACAAGGACTGCTATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAg  <  2:666561/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
ATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAG  >  NC_000913/2823690‑2823987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: