Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 18,681 A→T 6.8% intergenic (+26/‑34) nhaA → / → nhaR sodium‑proton antiporter/transcriptional activator of nhaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091318,6810AT 6.8% 374.9 / 8.7 133intergenic (+26/‑34)nhaA/nhaRsodium‑proton antiporter/transcriptional activator of nhaA
Reads supporting (aligned to +/- strand):  ref base A (75/48);  new base T (4/6);  total (79/54)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

GCCTTTGGTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTACCG  >  NC_000913/18533‑18829
                                                                                                                                                    |                                                                                                                                                    
gCCTTTGGTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATaaa                                                                                                                                                     >  1:700063/1‑150 (MQ=255)
 ccTTTGGTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAAc                                                                                                                                                    >  1:1546558/1‑150 (MQ=255)
  cTTTGGTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACg                                                                                                                                                   <  1:674532/150‑1 (MQ=255)
    ttGGTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTACTTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGc                                                                                                                                                 <  2:1072156/150‑1 (MQ=255)
      ggTAGCGTAGATCCAGCACTGATTAACTGGGCGACACTCTGTTTCCTGGTCGGTTCTATCACTTCGGCTGTAATTGGATAGAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATATACGGCTc                                                                                                                                               <  1:197240/150‑1 (MQ=255)
      ggTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTc                                                                                                                                               >  1:477818/1‑150 (MQ=255)
      ggTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTc                                                                                                                                               <  1:1709363/150‑1 (MQ=255)
       gTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGTTAATTGGATACAGCTGGTTACGCTTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTcc                                                                                                                                              >  1:83446/1‑150 (MQ=255)
            gTAGATCCAGAGCTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCtttt                                                                                                                                         >  2:633259/1‑150 (MQ=255)
            gTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACTGTTTACCGGGGAGACATAAAAGGCTCCCtgtt                                                                                                                                         >  2:95909/1‑150 (MQ=255)
             tAGATCAAGAACTGCTTAACTGGGCGAAACTCGGTAGCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCGTTTATGGCTccccggta                                                                                                                                        <  2:148691/150‑6 (MQ=255)
              aGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCa                                                                                                                                       >  2:1297369/1‑150 (MQ=255)
                aTCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCAtt                                                                                                                                     >  1:619733/1‑150 (MQ=255)
                aTCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCAtt                                                                                                                                     <  1:962569/150‑1 (MQ=255)
                  ccAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGACGGTTCTATCTCTTCGGCGGTAAATGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGt                                                                                                                                   >  2:1395826/1‑150 (MQ=255)
                     gAACTGATTTACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTAt                                                                                                                                >  2:1625367/1‑150 (MQ=255)
                       cgtGATTAACTGGGCGAAACTCGGTATCGTGGTGGGTTCTATCGCTTCGGCGGTAATTTGATACAGCTGGTTACGGGTTCGTTTGCGTCAATCAGTTTGTCAGGACGGTTTACCGGGTAGCCATATACGGCTCCCTTTTCATTGTTATCa                                                                                                                              <  1:1442215/148‑1 (MQ=255)
                       aCTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCa                                                                                                                              >  1:1394143/1‑150 (MQ=255)
                          gATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAggg                                                                                                                           >  2:867434/1‑150 (MQ=255)
                          gATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAggg                                                                                                                           >  1:352044/1‑150 (MQ=255)
                            ttAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGgag                                                                                                                         <  1:1075999/150‑1 (MQ=255)
                              aaCTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGgagag                                                                                                                       >  2:123574/1‑150 (MQ=255)
                               aCTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGgagaga                                                                                                                      >  1:563245/1‑150 (MQ=255)
                               aCTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGgagaga                                                                                                                      <  2:1069316/150‑1 (MQ=255)
                                cTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGaa                                                                                                                     >  1:543437/1‑150 (MQ=255)
                                    gCGAAACTCAGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGGTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATgg                                                                                                                 >  2:1421324/1‑149 (MQ=255)
                                     cGAAACTCGGTATCCTGGTCGGTTCTTTCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTGTACCGGGGAGCCATAAACGGCTCCCTTTTCAGTGTTATCAGGGAGAGAAATGAg                                                                                                                >  2:123643/1‑150 (MQ=255)
                                         aCTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATg                                                                                                            <  2:1434622/150‑1 (MQ=255)
                                         aCTAGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATg                                                                                                            <  2:695523/150‑1 (MQ=255)
                                          cTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGt                                                                                                           >  1:475581/1‑150 (MQ=255)
                                           tCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCGTTTATGGCTccccgg                                                                                                                                          >  2:1299858/1‑115 (MQ=255)
                                           tCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGtc                                                                                                          >  2:1387589/1‑150 (MQ=255)
                                             ggTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGtctc                                                                                                        <  2:1608791/150‑1 (MQ=255)
                                             ggTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGtctc                                                                                                        >  1:1252210/1‑150 (MQ=255)
                                              gTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCa                                                                                                       >  1:999655/1‑150 (MQ=255)
                                                aTCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCata                                                                                                     <  2:555621/150‑1 (MQ=255)
                                                 tCCTGGTCGGTTCTATCTCTTCTGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATAAGTTTGACAGGCCGGTTTACCGGGGCGCCATAAACGGCTCCCTGTTCAGTGTTATCAGGGAGAGAAATGAGCAGGTCTCatat                                                                                                    >  2:339983/1‑150 (MQ=255)
                                                 tCCTGGTCGGTTCTATCGCTTCGGCGGTAATTGGCTACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATTAGCATGTCTCatat                                                                                                    <  2:700664/150‑1 (MQ=255)
                                                  ccTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATc                                                                                                   <  1:694920/150‑1 (MQ=255)
                                                    tGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCaa                                                                                                 >  1:1184236/1‑150 (MQ=255)
                                                     ggTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAAt                                                                                                >  1:1276480/1‑150 (MQ=255)
                                                      gTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAAtt                                                                                               >  2:453677/1‑150 (MQ=255)
                                                       gcGGTTCTATCTCTTCGGAGGTAATTGGATACAGCTGGTTCCGCGGTCGTTTGCGTCCAACAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTa                                                                                              <  1:1059370/149‑1 (MQ=255)
                                                                 ctctTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTg                                                                                    >  2:92030/1‑150 (MQ=255)
                                                                 ctctTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTg                                                                                    <  2:312214/150‑1 (MQ=255)
                                                                  tctTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGt                                                                                   <  1:1244411/150‑1 (MQ=255)
                                                                     tCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTAtt                                                                                <  2:563245/150‑1 (MQ=255)
                                                                      cggcggTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTa                                                                               <  2:917671/150‑1 (MQ=255)
                                                                       ggcggcAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTAc                                                                              <  2:1369658/150‑1 (MQ=255)
                                                                        gcggTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACt                                                                             <  2:222789/150‑1 (MQ=255)
                                                                         cggTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACtt                                                                            >  1:755127/1‑150 (MQ=255)
                                                                             aaTTGGATACAGCGGGTTACGCGTTCGTTTGCGTCACTGAGTTTGACAGGACGGTTTACCGGGGCGCCATAAACTGCTCCCTTATCAAAGATTTCAGGGAGATAAATGAGCATATCTAATAGCAATAACAAAAAAATGTATTACttaagc                                                                        >  2:163962/1‑146 (MQ=255)
                                                                               ttGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCa                                                                      >  1:686685/1‑150 (MQ=255)
                                                                                   aTACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTc                                                                  <  1:730024/150‑1 (MQ=255)
                                                                                      cAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCtat                                                               >  1:803045/1‑150 (MQ=255)
                                                                                              tACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAgg                                                       <  2:379231/150‑1 (MQ=255)
                                                                                              tACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAgg                                                       >  1:1126312/1‑150 (MQ=255)
                                                                                                  cgttcgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTcc                                                   >  2:80090/1‑150 (MQ=255)
                                                                                                  cgttcgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTcc                                                   >  1:1557662/1‑150 (MQ=255)
                                                                                                  cgttcgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTAGGGCATGTCTATAAAGAAGGTTcc                                                   >  1:1692871/1‑150 (MQ=255)
                                                                                                   gttcgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATATAGAAGGTTCCg                                                  >  2:1253871/1‑150 (MQ=255)
                                                                                                       gttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGt                                              >  1:986878/1‑150 (MQ=255)
                                                                                                         ttGCGTCCATCAGTTTGACAGGACGGTTTACGGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTg                                            <  2:83446/150‑1 (MQ=255)
                                                                                                          tGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTAATATCAATTAAAACCAATTGTATTACTTCTGTAATGTCTATAAAGAAGGTTCCGTGTTTgg                                           >  1:510833/1‑150 (MQ=255)
                                                                                                            cGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGgcg                                         >  2:411924/1‑150 (MQ=255)
                                                                                                            cGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGgcg                                         >  1:1346391/1‑150 (MQ=255)
                                                                                                            cGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCAGGTCTCATATCAATTACAACCACTTGTAGTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGgcg                                         >  2:1727023/1‑150 (MQ=255)
                                                                                                            cGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCANNNACGNNTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGgcg                                         >  2:1733619/1‑150 (MQ=255)
                                                                                                             gTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGgcgc                                        <  1:703042/150‑1 (MQ=255)
                                                                                                             gTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGgcgc                                        <  1:829755/150‑1 (MQ=255)
                                                                                                                cATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGTAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGTTTCCGTGTTTGGCGCAGc                                     >  1:1088399/1‑150 (MQ=255)
                                                                                                                cATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGc                                     >  2:648622/1‑150 (MQ=255)
                                                                                                                cATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTGTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGc                                     <  1:508878/150‑1 (MQ=255)
                                                                                                                cATCAGTTTGACAGGACGGATTACCGGGGAGCCATAAACGGCTCCCATTTCATTGTTTTCAGGGAGAGAAATGAGCATGTCACAAATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATATAGAAGGTTCCGTGGTTGGCGCAGc                                     >  2:504503/1‑150 (MQ=255)
                                                                                                                 aTCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCg                                    >  2:353064/1‑150 (MQ=255)
                                                                                                                 aTCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAGGTTCCGTGGTTTGCGCAGccg                                   >  2:901275/1‑148 (MQ=255)
                                                                                                                  tCAGTTTGACAGGACGGGTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCgg                                   <  2:971274/150‑1 (MQ=255)
                                                                                                                   cAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGa                                  >  2:628709/1‑150 (MQ=255)
                                                                                                                          aCAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCtt                           >  1:1148766/1‑150 (MQ=255)
                                                                                                                           cAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCttt                          <  1:709932/150‑1 (MQ=255)
                                                                                                                             ggACTGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCtttat                        <  2:392299/150‑1 (MQ=255)
                                                                                                                             ggACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCtttat                        >  1:431234/1‑150 (MQ=255)
                                                                                                                             ggACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCtttat                        >  2:1169556/1‑150 (MQ=255)
                                                                                                                             ggACGGTTTACCGGGGAGCCATAAACGGCTCCCGTTTCATTGTTATGAGGGAGAGAAATGAGCATGTCTCATTTCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCtttat                        <  2:1528608/150‑1 (MQ=255)
                                                                                                                               aCGGTTTACCGGGGAGCCATAATCGGCTCCCTGTTCATTGTTATCAGGGAGAGAAATGAGCATTTCTCATATCAATTACAACCAATTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCtttattt                      <  2:1321476/150‑1 (MQ=255)
                                                                                                                               aCGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCtttattt                      <  2:398964/150‑1 (MQ=255)
                                                                                                                               aCGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCtttattt                      <  2:388404/150‑1 (MQ=255)
                                                                                                                                  gTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAAc                   >  2:809455/1‑150 (MQ=255)
                                                                                                                                  gTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTACTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGATGGCGCAGCGGAGGCGCTTTATTTAAc                   <  2:1003212/150‑1 (MQ=255)
                                                                                                                                       ccGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCcac              <  1:62216/150‑1 (MQ=255)
                                                                                                                                       ccGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCcac              <  1:1710804/150‑1 (MQ=255)
                                                                                                                                       ccGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTGGGCGCAGCGGAGGCGCTTTATTTAACTCcac              <  1:255536/150‑1 (MQ=255)
                                                                                                                                       aaagggAGCCGTTTATGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCAGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCcac              >  1:999225/4‑150 (MQ=255)
                                                                                                                                         ggggAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACaa            >  2:292124/1‑150 (MQ=255)
                                                                                                                                          gggAGCCGTTTATGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACCACCACTTGTATTACTTCTGGAATGACTATAAAGAAGGTTCCGTGGTTGGAGCAGCGGAGGCGCTTTATTTAACTCCACaaa           >  1:92766/1‑150 (MQ=255)
                                                                                                                                               ccATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCAtt      <  2:475581/150‑1 (MQ=255)
                                                                                                                                                cATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTAATTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTa     >  1:58744/1‑150 (MQ=255)
                                                                                                                                                 aTAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTGATTTAACTCCACAAACCATTAc    >  2:832660/1‑150 (MQ=255)
                                                                                                                                                  tAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTAcc   <  2:803045/150‑1 (MQ=255)
                                                                                                                                                   aaaCGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTACCg  <  1:61807/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCTTTGGTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTACCG  >  NC_000913/18533‑18829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: