Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,839,321 C→G 12.8% intergenic (‑57/‑203) ascG ← / → ascF asc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,839,3210CG12.8% 313.7 / 35.1 117intergenic (‑57/‑203)ascG/ascFasc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
Reads supporting (aligned to +/- strand):  ref base C (51/48);  new base G (5/10);  total (57/60)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01

Only 100 of 119 total aligned reads displayed.

TCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCG  >  NC_000913/2839173‑2839469
                                                                                                                                                    |                                                                                                                                                    
tcCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTcc                                                                                                                                                     <  2:1207652/150‑1 (MQ=255)
          cGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATc                                                                                                                                           <  2:210914/150‑1 (MQ=255)
          cGTAGCCATTACCTGAAAGCACGAGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATc                                                                                                                                           <  2:131152/150‑1 (MQ=255)
            tAGCCATTACCTGAAATCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcgg                                                                                                                                         <  1:128233/150‑1 (MQ=255)
             aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                        <  1:417076/150‑1 (MQ=255)
             aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                        <  1:1646394/150‑1 (MQ=255)
             aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggc                                                                                                                                        <  2:451754/150‑1 (MQ=255)
             aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGGCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                        <  1:1530050/150‑1 (MQ=255)
              gCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggca                                                                                                                                       <  1:1194025/150‑2 (MQ=255)
              gCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcg                                                                                                                                       >  2:377129/1‑150 (MQ=255)
                cATTACCTTAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTATCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCggg                                                                                                                                     <  2:398782/150‑1 (MQ=255)
                cATTACCTGCACGCACGCGGGAAACGGTCGCGTTTGACACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcgaa                                                                                                                                     <  2:1077701/150‑3 (MQ=255)
                cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCggg                                                                                                                                     <  2:414944/150‑1 (MQ=255)
                cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTCGCCGATTGTGCAAGGACAATcggcaaa                                                                                                                                     <  1:1526273/150‑4 (MQ=255)
                cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTCGCCGATTGTGCAAGGACAATcggcaaa                                                                                                                                     <  1:1532850/150‑4 (MQ=255)
                cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATAGTCGTCATCATTTTCATCCCTTTAGACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCGTGCACAAGCGGCggg                                                                                                                                     >  2:1366533/1‑150 (MQ=255)
                cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCATCTCCACTTCCAGCATCGTCGGCATCATTTTCATCCCTTTTCACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCggg                                                                                                                                     >  2:623645/1‑150 (MQ=255)
                   tACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGaaa                                                                                                                                  <  1:528500/150‑1 (MQ=255)
                   tACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGaaa                                                                                                                                  >  1:1026733/1‑150 (MQ=255)
                    aCCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGaaaa                                                                                                                                 <  1:26552/150‑1 (MQ=255)
                      cTGAACGCACGAGGGATACGGTCGCTTTTGAAACCCCGGCGCGCTGCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCGTTTACACGCAATCAACGCAGTGTACTGCAGCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAat                                                                                                                               <  2:174711/150‑1 (MQ=255)
                      cTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAat                                                                                                                               >  2:694426/1‑150 (MQ=255)
                          aaGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTAGCCACTTCCAGCATCGTCGTCATCATTTTCCTCCCATTACCAGCAATCAACGCAGTGTACAGCCCCCGTTGCCGCTTGTGCTTGCACAATGGGCGGGTAAAATAtta                                                                                                                           >  2:1281203/1‑149 (MQ=255)
                           aGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCa                                                                                                                          <  2:1427890/150‑1 (MQ=255)
                            gCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAg                                                                                                                         >  1:998901/1‑150 (MQ=255)
                            gCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAg                                                                                                                         <  2:86165/150‑1 (MQ=255)
                            gCACGCGGGAAACGGGCGCTTTTGAAACCCCGGCGCGCTTCGCCACATCGAGCATCGTCGTGATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAg                                                                                                                         <  2:743524/150‑1 (MQ=255)
                               cgcgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTg                                                                                                                      <  2:477238/150‑1 (MQ=255)
                                     aaaCGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACCGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGt                                                                                                                <  1:970495/150‑1 (MQ=255)
                                          gTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTcac                                                                                                           >  2:1005230/1‑150 (MQ=255)
                                          gTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTcac                                                                                                           <  1:827449/150‑1 (MQ=255)
                                          gTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTACTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTcac                                                                                                           >  1:422845/1‑150 (MQ=255)
                                             gCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaaa                                                                                                        >  1:196191/1‑150 (MQ=255)
                                                  tGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaa                                                                                                   <  1:1628565/150‑1 (MQ=255)
                                                    aaaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaaa                                                                                                 >  1:1126762/1‑150 (MQ=255)
                                                     aaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGGACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATTTaaaaa                                                                                                >  2:309953/1‑150 (MQ=255)
                                                     aaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATACCTTTACACGCAATCAACGCAGTGGACTGCAGCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGAGCCCTGTTTCAAAAATATaaaaa                                                                                                >  1:36756/1‑150 (MQ=255)
                                                       ccccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAAt                                                                                              <  2:725654/150‑1 (MQ=255)
                                                        cccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATg                                                                                             <  1:1072447/150‑1 (MQ=255)
                                                        cccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATg                                                                                             <  1:417393/150‑1 (MQ=255)
                                                           ggCGCGCTTCGCCACTTCCAGTATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAAc                                                                                          <  1:1389101/150‑1 (MQ=255)
                                                           ggCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAAc                                                                                          >  1:1245541/1‑150 (MQ=255)
                                                            gcgcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACa                                                                                         >  1:661340/1‑150 (MQ=255)
                                                              gcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGACCAAt                                                                                       >  1:276688/1‑150 (MQ=255)
                                                              gcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATAAACGCAGTGTACTGATCCGTTTGCCTATTGTACTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAt                                                                                       <  1:1549821/150‑1 (MQ=255)
                                                               cgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGTTTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAtt                                                                                      <  2:135809/150‑1 (MQ=255)
                                                                gcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCACTCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTc                                                                                     <  1:906594/150‑1 (MQ=255)
                                                                 cTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCa                                                                                    >  2:57906/1‑150 (MQ=255)
                                                                    cGCCACAACCAGCATCGTCGTCATCATTTTCATCCCTTTACACCCAATCAACGCAGTGGACTGCACCGTTTGCCGAGTGCCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActc                                                                                 <  2:106739/150‑1 (MQ=255)
                                                                     gCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActct                                                                                <  1:1583111/150‑1 (MQ=255)
                                                                       cACTTCCATCATCGTCGTCATCATTTTCATCTCTTTACACGCAATCTAGGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActctct                                                                              <  1:191117/150‑1 (MQ=255)
                                                                           tCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCt                                                                          <  1:1423177/150‑1 (MQ=255)
                                                                           tCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCt                                                                          >  2:1366584/1‑150 (MQ=255)
                                                                              aGCATCGTCGTCATCATTTTCATCCCTTTACCCGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGACCAAATCACTCTCTTGCTTAt                                                                       >  1:301392/1‑150 (MQ=255)
                                                                                cATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTAttt                                                                     <  1:1088461/150‑1 (MQ=255)
                                                                                cATAGTCGTAATCATTTTCTTACCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCTCAATCGGCGGGAATAATATTCAGGTGTCCGGTTTCACAAATATAAAAAATGATCTATTCACTCTCTTGCTTAttt                                                                     <  2:1651406/150‑1 (MQ=255)
                                                                                 aTCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTa                                                                    >  1:216941/1‑150 (MQ=255)
                                                                                    gtcgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTg                                                                 <  1:572301/150‑1 (MQ=255)
                                                                                        tcatcaTTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACaa                                                             >  2:1055513/1‑150 (MQ=255)
                                                                                        tcatcaTTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACaa                                                             >  1:938521/1‑150 (MQ=255)
                                                                                        tcatcaTTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACaa                                                             >  2:1287548/1‑150 (MQ=255)
                                                                                         catcttTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAAc                                                            <  1:177634/150‑1 (MQ=255)
                                                                                         catcatTTTCATCCCTTTACCCGCAATCAACGCAGTGTACTGCACCGTTTGCCGCTTGTCCTTGCACTATCGGCGGGAAAAATATTCAGGTGACCGGATTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAAc                                                            <  2:727785/150‑1 (MQ=255)
                                                                                           tcatTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTAAGGTGACAGGTTTCACAAATATAAAAAATGAACAATTCACTCTATTGCTTATTTAGTGACAACTa                                                          >  1:1506585/1‑150 (MQ=255)
                                                                                             atTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTAtt                                                        >  2:115481/1‑150 (MQ=255)
                                                                                              ttttCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTc                                                       <  2:1026733/150‑1 (MQ=255)
                                                                                                   aTCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGAt                                                  >  1:1582377/1‑150 (MQ=255)
                                                                                                    tCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGAtt                                                 >  2:1282293/1‑150 (MQ=255)
                                                                                                         ttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgtg                                            <  2:213854/150‑1 (MQ=255)
                                                                                                            cacGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTTTGaaa                                         >  2:281041/1‑150 (MQ=255)
                                                                                                            cacGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGaaa                                         >  2:1045977/1‑150 (MQ=255)
                                                                                                             acGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAc                                        >  1:1162406/1‑150 (MQ=255)
                                                                                                              cGCAATCAACGCAGTGTACTGCACCGTTTGCCGTTTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTGACTCTGTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAcc                                       <  2:1296298/150‑1 (MQ=255)
                                                                                                              cGCAATCAACGAAGTGTACTGCACCTTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGAACGGGTTCACAAATATAAAAAATGAACAATTCACTCTATTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAcc                                       <  2:727032/150‑1 (MQ=255)
                                                                                                                   tCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGttt                                  <  2:1666365/150‑1 (MQ=255)
                                                                                                                    cAACGCAGTGTACTGCACCGTTTGTCGATTGTCCTTGCACAATCGGCGGGAAATATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                 <  1:1674105/150‑1 (MQ=255)
                                                                                                                    cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                 >  1:1623319/1‑150 (MQ=255)
                                                                                                                    cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                 >  2:671754/1‑150 (MQ=255)
                                                                                                                     aaCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCt                                >  2:328748/1‑150 (MQ=255)
                                                                                                                      aCGCAGTGTAATGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCtt                               <  2:870731/150‑1 (MQ=255)
                                                                                                                         cAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAAt                            <  2:647052/150‑1 (MQ=255)
                                                                                                                           gtgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAAGAATTCCCTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTc                          >  2:1454275/1‑150 (MQ=255)
                                                                                                                            tgtACTGCACCGTTTGCCTATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTcc                         <  1:623645/150‑1 (MQ=255)
                                                                                                                             gtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACTAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCAGGATTTTGTGAAACCGGTTTCTTAATTCCg                        <  1:283497/150‑1 (MQ=255)
                                                                                                                                cTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGATACCGGTTACTTAATTCCCttt                     >  2:849654/1‑150 (MQ=255)
                                                                                                                                cTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGttt                     >  1:556025/1‑150 (MQ=255)
                                                                                                                                  gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCa                   >  2:498902/1‑150 (MQ=255)
                                                                                                                                  gCACCGTTTGCCGATTGTACTTGAACAATCGGCGGGAAAAATATTCATGTGACCGGTTTCACAAATATAAAAAATTAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCa                   <  1:959248/150‑1 (MQ=255)
                                                                                                                                     ccGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCa                >  2:1322886/1‑150 (MQ=255)
                                                                                                                                       gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACTACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATc              <  2:422845/150‑1 (MQ=255)
                                                                                                                                       gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTAATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATc              >  1:807499/1‑150 (MQ=255)
                                                                                                                                          cgCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGc           >  2:1249453/2‑150 (MQ=255)
                                                                                                                                           gCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa          >  1:407148/1‑150 (MQ=255)
                                                                                                                                           gCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa          >  1:95187/1‑150 (MQ=255)
                                                                                                                                             cGATTGTGCAAGGACAATCGGCATCAAAAAAATTAAGGTGACCTAATTAAAAAATATAAAAAATGAACAATTAACTCTCATTATAATTTAGATACAAAAATTCATGATTTTGTGAAACCGGTTTCTTAATTACTTTTCAGCATCGGCAtt        >  2:198661/1‑150 (MQ=255)
                                                                                                                                             cGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCAtt        >  1:381024/1‑150 (MQ=255)
                                                                                                                                               aTTGTCCTTGCACAATCGGCTGGAAAAATATTAAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCAtttt      <  2:91582/150‑1 (MQ=255)
                                                                                                                                                   tCCTTGCACAATCGGCGGGAAAAATATTCAGTTGACCTGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg  <  1:470786/150‑1 (MQ=255)
                                                                                                                                                   tCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg  <  1:1282293/150‑1 (MQ=255)
                                                                                                                                                   tCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg  <  1:1296586/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCG  >  NC_000913/2839173‑2839469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: