Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,839,323 T→A 12.0% intergenic (‑59/‑201) ascG ← / → ascF asc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,839,3230TA12.0% 275.9 / 27.8 117intergenic (‑59/‑201)ascG/ascFasc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
Reads supporting (aligned to +/- strand):  ref base T (52/50);  new base A (4/10);  total (57/60)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.49e-02

Only 100 of 117 total aligned reads displayed.

TGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCG  >  NC_000913/2839180‑2839469
                                                                                                                                               |                                                                                                                                                  
tGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGTATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGcaca                                                                                                                                              >  2:1389101/1‑150 (MQ=255)
   cGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATc                                                                                                                                           <  2:210914/150‑1 (MQ=255)
   cGTAGCCATTACCTGAAAGCACGAGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATc                                                                                                                                           <  2:131152/150‑1 (MQ=255)
     tAGCCATTACCTGAAATCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcgg                                                                                                                                         <  1:128233/150‑1 (MQ=255)
      aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                        <  1:1646394/150‑1 (MQ=255)
      aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                        <  1:417076/150‑1 (MQ=255)
      aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggc                                                                                                                                        <  2:451754/150‑1 (MQ=255)
      aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGGCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                        <  1:1530050/150‑1 (MQ=255)
       gCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggca                                                                                                                                       <  1:1194025/150‑2 (MQ=255)
       gCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcg                                                                                                                                       >  2:377129/1‑150 (MQ=255)
        ccATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcgg                                                                                                                                      >  1:762510/1‑150 (MQ=255)
         cATTACCTTAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTATCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCggg                                                                                                                                     <  2:398782/150‑1 (MQ=255)
         cATTACCTGCACGCACGCGGGAAACGGTCGCGTTTGACACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcgaa                                                                                                                                     <  2:1077701/150‑3 (MQ=255)
         cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCggg                                                                                                                                     <  2:414944/150‑1 (MQ=255)
         cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTCGCCGATTGTGCAAGGACAATcggcaaa                                                                                                                                     <  1:1526273/150‑4 (MQ=255)
         cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTCGCCGATTGTGCAAGGACAATcggcaaa                                                                                                                                     <  1:1532850/150‑4 (MQ=255)
         cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATAGTCGTCATCATTTTCATCCCTTTAGACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCGTGCACAAGCGGCggg                                                                                                                                     >  2:1366533/1‑150 (MQ=255)
         cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCATCTCCACTTCCAGCATCGTCGGCATCATTTTCATCCCTTTTCACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCggg                                                                                                                                     >  2:623645/1‑150 (MQ=255)
            tACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGaaa                                                                                                                                  <  1:528500/150‑1 (MQ=255)
            tACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGaaa                                                                                                                                  >  1:1026733/1‑150 (MQ=255)
             aCCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGaaaa                                                                                                                                 <  1:26552/150‑1 (MQ=255)
              ccTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAAACGGCGGGaaaaa                                                                                                                                >  1:1671957/1‑150 (MQ=255)
               cTGAACGCACGAGGGATACGGTCGCTTTTGAAACCCCGGCGCGCTGCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCGTTTACACGCAATCAACGCAGTGTACTGCAGCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAat                                                                                                                               <  2:174711/150‑1 (MQ=255)
               cTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAat                                                                                                                               >  2:694426/1‑150 (MQ=255)
                   aaGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTAGCCACTTCCAGCATCGTCGTCATCATTTTCCTCCCATTACCAGCAATCAACGCAGTGTACAGCCCCCGTTGCCGCTTGTGCTTGCACAATGGGCGGGTAAAATAtta                                                                                                                           >  2:1281203/1‑149 (MQ=255)
                    aGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCa                                                                                                                          <  2:1427890/150‑1 (MQ=255)
                     gCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAg                                                                                                                         <  2:86165/150‑1 (MQ=255)
                     gCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAg                                                                                                                         >  1:998901/1‑150 (MQ=255)
                     gCACGCGGGAAACGGGCGCTTTTGAAACCCCGGCGCGCTTCGCCACATCGAGCATCGTCGTGATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAg                                                                                                                         <  2:743524/150‑1 (MQ=255)
                        cgcgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTg                                                                                                                      <  2:477238/150‑1 (MQ=255)
                              aaaCGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACCGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGt                                                                                                                <  1:970495/150‑1 (MQ=255)
                                 cGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTc                                                                                                             <  2:456529/150‑1 (MQ=255)
                                   gTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTcac                                                                                                           <  1:827449/150‑1 (MQ=255)
                                   gTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTcac                                                                                                           >  2:1005230/1‑150 (MQ=255)
                                   gTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTACTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTcac                                                                                                           >  1:422845/1‑150 (MQ=255)
                                      gCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaaa                                                                                                        >  2:1192788/1‑150 (MQ=255)
                                      gCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaaa                                                                                                        >  1:196191/1‑150 (MQ=255)
                                       cTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAat                                                                                                       <  1:238567/150‑1 (MQ=255)
                                           tGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaa                                                                                                   >  2:666843/1‑150 (MQ=255)
                                           tGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaa                                                                                                   <  1:1628565/150‑1 (MQ=255)
                                             aaaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaaa                                                                                                 >  1:1126762/1‑150 (MQ=255)
                                              aaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGGACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATTTaaaaa                                                                                                >  2:309953/1‑150 (MQ=255)
                                              aaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATACCTTTACACGCAATCAACGCAGTGGACTGCAGCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGAGCCCTGTTTCAAAAATATaaaaa                                                                                                >  1:36756/1‑150 (MQ=255)
                                                ccccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAAt                                                                                              <  2:725654/150‑1 (MQ=255)
                                                 cccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATg                                                                                             <  1:1072447/150‑1 (MQ=255)
                                                    ggCGCGCTTCGCCACTTCCAGTATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAAc                                                                                          <  1:1389101/150‑1 (MQ=255)
                                                    ggCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAAc                                                                                          >  1:1245541/1‑150 (MQ=255)
                                                     gcgcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACa                                                                                         >  1:661340/1‑150 (MQ=255)
                                                       gcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGACCAAt                                                                                       >  1:276688/1‑150 (MQ=255)
                                                       gcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATAAACGCAGTGTACTGATCCGTTTGCCTATTGTACTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAt                                                                                       <  1:1549821/150‑1 (MQ=255)
                                                        cgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGTTTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAtt                                                                                      <  2:135809/150‑1 (MQ=255)
                                                         gcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCACTCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTc                                                                                     <  1:906594/150‑1 (MQ=255)
                                                          cTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCa                                                                                    >  2:57906/1‑150 (MQ=255)
                                                             cGCCACAACCAGCATCGTCGTCATCATTTTCATCCCTTTACACCCAATCAACGCAGTGGACTGCACCGTTTGCCGAGTGCCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActc                                                                                 <  2:106739/150‑1 (MQ=255)
                                                              gCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActct                                                                                <  1:1583111/150‑1 (MQ=255)
                                                                cACTTCCATCATCGTCGTCATCATTTTCATCTCTTTACACGCAATCTAGGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActctct                                                                              <  1:191117/150‑1 (MQ=255)
                                                                    tCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCt                                                                          >  2:1366584/1‑150 (MQ=255)
                                                                       aGCATCGTCGTCATCATTTTCATCCCTTTACCCGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGACCAAATCACTCTCTTGCTTAt                                                                       >  1:301392/1‑150 (MQ=255)
                                                                         cATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTAttt                                                                     <  1:1088461/150‑1 (MQ=255)
                                                                          aTCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTa                                                                    >  1:216941/1‑150 (MQ=255)
                                                                             gtcgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTg                                                                 <  1:377129/150‑1 (MQ=255)
                                                                             gtcgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTg                                                                 <  1:572301/150‑1 (MQ=255)
                                                                                gtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACa                                                              >  1:1402767/1‑150 (MQ=255)
                                                                                 tcatcaTTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACaa                                                             >  2:1287548/1‑150 (MQ=255)
                                                                                 tcatcaTTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACaa                                                             >  1:938521/1‑150 (MQ=255)
                                                                                 tcatcaTTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACaa                                                             >  2:1055513/1‑150 (MQ=255)
                                                                                  catcatTTTCATCCCTTTACCCGCAATCAACGCAGTGTACTGCACCGTTTGCCGCTTGTCCTTGCACTATCGGCGGGAAAAATATTCAGGTGACCGGATTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAAc                                                            <  2:727785/150‑1 (MQ=255)
                                                                                    tcatTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTAAGGTGACAGGTTTCACAAATATAAAAAATGAACAATTCACTCTATTGCTTATTTAGTGACAACTa                                                          >  1:1506585/1‑150 (MQ=255)
                                                                                      atTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTAtt                                                        >  2:115481/1‑150 (MQ=255)
                                                                                       ttttCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTc                                                       <  2:1026733/150‑1 (MQ=255)
                                                                                             tCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGAtt                                                 >  2:1282293/1‑150 (MQ=255)
                                                                                              cccTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTTGTACAACTATTCAATAtttt                                               >  2:1300734/1‑150 (MQ=255)
                                                                                                  ttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgtg                                            <  2:213854/150‑1 (MQ=255)
                                                                                                     cacGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCCCAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGTCAACTTTTCATGATTTTGTGaaa                                         >  2:319271/1‑150 (MQ=255)
                                                                                                     cacGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGaaa                                         >  2:1045977/1‑150 (MQ=255)
                                                                                                      acGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAc                                        >  1:1162406/1‑150 (MQ=255)
                                                                                                       cGCAATCAACGCAGTGTACTGCACCGTTTGCCGTTTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTGACTCTGTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAcc                                       <  2:1296298/150‑1 (MQ=255)
                                                                                                       cGCAATCAACGAAGTGTACTGCACCTTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGAACGGGTTCACAAATATAAAAAATGAACAATTCACTCTATTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAcc                                       <  2:727032/150‑1 (MQ=255)
                                                                                                            tCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGttt                                  <  2:1666365/150‑1 (MQ=255)
                                                                                                             cAACGCAGTGTACTGCACCGTTTGTCGATTGTCCTTGCACAATCGGCGGGAAATATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                 <  1:1674105/150‑1 (MQ=255)
                                                                                                             cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                 >  2:671754/1‑150 (MQ=255)
                                                                                                             cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                 >  1:1623319/1‑150 (MQ=255)
                                                                                                              aaCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCt                                >  2:328748/1‑150 (MQ=255)
                                                                                                               aCGCAGTGTAATGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCtt                               <  2:870731/150‑1 (MQ=255)
                                                                                                                    gtgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAAGAATTCCCTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTc                          >  2:1454275/1‑150 (MQ=255)
                                                                                                                      gtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGATTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCg                        >  1:461562/1‑150 (MQ=255)
                                                                                                                         cTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGATACCGGTTACTTAATTCCCttt                     >  2:849654/1‑150 (MQ=255)
                                                                                                                         cTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGttt                     >  1:556025/1‑150 (MQ=255)
                                                                                                                           gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCa                   >  2:498902/1‑150 (MQ=255)
                                                                                                                           gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTAATAAGTGACAACTTTTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTATCa                   >  2:181084/1‑150 (MQ=255)
                                                                                                                           gCACCGTTTGCCGATTGTACTTGAACAATCGGCGGGAAAAATATTCATGTGACCGGTTTCACAAATATAAAAAATTAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCa                   <  1:959248/150‑1 (MQ=255)
                                                                                                                                gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTAATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATc              >  1:807499/1‑150 (MQ=255)
                                                                                                                                    gCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa          >  1:407148/1‑150 (MQ=255)
                                                                                                                                      cGATTGTGCAAGGACAATCGGCATCAAAAAAATTAAGGTGACCTAATTAAAAAATATAAAAAATGAACAATTAACTCTCATTATAATTTAGATACAAAAATTCATGATTTTGTGAAACCGGTTTCTTAATTACTTTTCAGCATCGGCAtt        >  2:198661/1‑150 (MQ=255)
                                                                                                                                      cGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCAtt        >  1:381024/1‑150 (MQ=255)
                                                                                                                                        aTTGTCCTTGCACAATCGGCTGGAAAAATATTAAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCAtttt      <  2:91582/150‑1 (MQ=255)
                                                                                                                                           gTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCAGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTcc   >  1:1548239/1‑150 (MQ=255)
                                                                                                                                            tCCTTGCACAATCGGCGGGAAAAATATTCAGTTGACCTGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg  <  1:470786/150‑1 (MQ=255)
                                                                                                                                            tCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg  <  1:1282293/150‑1 (MQ=255)
                                                                                                                                            tCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg  <  1:1296586/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCG  >  NC_000913/2839180‑2839469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: