Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,839,324 T→A 12.0% intergenic (‑60/‑200) ascG ← / → ascF asc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,839,3240TA12.0% 288.0 / 27.8 117intergenic (‑60/‑200)ascG/ascFasc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
Reads supporting (aligned to +/- strand):  ref base T (53/50);  new base A (4/10);  total (57/60)
Fisher's exact test for biased strand distribution p-value = 1.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.07e-01

Only 100 of 119 total aligned reads displayed.

TGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCAC  >  NC_000913/2839180‑2839473
                                                                                                                                                |                                                                                                                                                     
   cGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATc                                                                                                                                               <  2:210914/150‑1 (MQ=255)
     tAGCCATTACCTGAAATCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcgg                                                                                                                                             <  1:128233/150‑1 (MQ=255)
      aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                            <  1:1646394/150‑1 (MQ=255)
      aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                            <  1:417076/150‑1 (MQ=255)
      aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggc                                                                                                                                            <  2:451754/150‑1 (MQ=255)
      aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGGCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                            <  1:1530050/150‑1 (MQ=255)
       gCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggca                                                                                                                                           <  1:1194025/150‑2 (MQ=255)
       gCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcg                                                                                                                                           >  2:377129/1‑150 (MQ=255)
         cATTACCTTAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTATCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCggg                                                                                                                                         <  2:398782/150‑1 (MQ=255)
         cATTACCTGCACGCACGCGGGAAACGGTCGCGTTTGACACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcgaa                                                                                                                                         <  2:1077701/150‑3 (MQ=255)
         cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCggg                                                                                                                                         <  2:414944/150‑1 (MQ=255)
         cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTCGCCGATTGTGCAAGGACAATcggcaaa                                                                                                                                         <  1:1526273/150‑4 (MQ=255)
         cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTCGCCGATTGTGCAAGGACAATcggcaaa                                                                                                                                         <  1:1532850/150‑4 (MQ=255)
         cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATAGTCGTCATCATTTTCATCCCTTTAGACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCGTGCACAAGCGGCggg                                                                                                                                         >  2:1366533/1‑150 (MQ=255)
         cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCATCTCCACTTCCAGCATCGTCGGCATCATTTTCATCCCTTTTCACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCggg                                                                                                                                         >  2:623645/1‑150 (MQ=255)
            tACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGaaa                                                                                                                                      >  1:1026733/1‑150 (MQ=255)
            tACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGaaa                                                                                                                                      <  1:528500/150‑1 (MQ=255)
             aCCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGaaaa                                                                                                                                     <  1:26552/150‑1 (MQ=255)
               cTGAACGCACGAGGGATACGGTCGCTTTTGAAACCCCGGCGCGCTGCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCGTTTACACGCAATCAACGCAGTGTACTGCAGCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAat                                                                                                                                   <  2:174711/150‑1 (MQ=255)
               cTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAat                                                                                                                                   >  2:694426/1‑150 (MQ=255)
                   aaGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTAGCCACTTCCAGCATCGTCGTCATCATTTTCCTCCCATTACCAGCAATCAACGCAGTGTACAGCCCCCGTTGCCGCTTGTGCTTGCACAATGGGCGGGTAAAATAtta                                                                                                                               >  2:1281203/1‑149 (MQ=255)
                    aGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCa                                                                                                                              <  2:1427890/150‑1 (MQ=255)
                     gCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAg                                                                                                                             <  2:86165/150‑1 (MQ=255)
                     gCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAg                                                                                                                             >  1:998901/1‑150 (MQ=255)
                     gCACGCGGGAAACGGGCGCTTTTGAAACCCCGGCGCGCTTCGCCACATCGAGCATCGTCGTGATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAg                                                                                                                             <  2:743524/150‑1 (MQ=255)
                        cgcgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTg                                                                                                                          <  2:477238/150‑1 (MQ=255)
                              aaaCGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACCGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGt                                                                                                                    <  1:970495/150‑1 (MQ=255)
                                   gTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTcac                                                                                                               <  1:827449/150‑1 (MQ=255)
                                   gTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTcac                                                                                                               >  2:1005230/1‑150 (MQ=255)
                                   gTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTACTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTcac                                                                                                               >  1:422845/1‑150 (MQ=255)
                                      gCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaaa                                                                                                            >  1:196191/1‑150 (MQ=255)
                                           tGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaa                                                                                                       <  1:1628565/150‑1 (MQ=255)
                                             aaaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaaa                                                                                                     >  1:1126762/1‑150 (MQ=255)
                                              aaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGGACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATTTaaaaa                                                                                                    >  2:309953/1‑150 (MQ=255)
                                              aaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATACCTTTACACGCAATCAACGCAGTGGACTGCAGCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGAGCCCTGTTTCAAAAATATaaaaa                                                                                                    >  1:36756/1‑150 (MQ=255)
                                                ccccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAAt                                                                                                  <  2:725654/150‑1 (MQ=255)
                                                 cccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATg                                                                                                 <  1:1072447/150‑1 (MQ=255)
                                                 cccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATg                                                                                                 <  1:417393/150‑1 (MQ=255)
                                                    ggCGCGCTTCGCCACTTCCAGTATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAAc                                                                                              <  1:1389101/150‑1 (MQ=255)
                                                    ggCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAAc                                                                                              >  1:1245541/1‑150 (MQ=255)
                                                     gcgcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACa                                                                                             >  1:661340/1‑150 (MQ=255)
                                                       gcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGACCAAt                                                                                           >  1:276688/1‑150 (MQ=255)
                                                       gcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATAAACGCAGTGTACTGATCCGTTTGCCTATTGTACTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAt                                                                                           <  1:1549821/150‑1 (MQ=255)
                                                        cgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGTTTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAtt                                                                                          <  2:135809/150‑1 (MQ=255)
                                                         gcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCACTCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTc                                                                                         <  1:906594/150‑1 (MQ=255)
                                                          cTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCa                                                                                        >  2:57906/1‑150 (MQ=255)
                                                             cGCCACAACCAGCATCGTCGTCATCATTTTCATCCCTTTACACCCAATCAACGCAGTGGACTGCACCGTTTGCCGAGTGCCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActc                                                                                     <  2:106739/150‑1 (MQ=255)
                                                              gCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActct                                                                                    <  1:1583111/150‑1 (MQ=255)
                                                                cACTTCCATCATCGTCGTCATCATTTTCATCTCTTTACACGCAATCTAGGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActctct                                                                                  <  1:191117/150‑1 (MQ=255)
                                                                    tCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCt                                                                              >  2:1366584/1‑150 (MQ=255)
                                                                    tCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCt                                                                              <  1:1423177/150‑1 (MQ=255)
                                                                       aGCATCGTCGTCATCATTTTCATCCCTTTACCCGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGACCAAATCACTCTCTTGCTTAt                                                                           >  1:301392/1‑150 (MQ=255)
                                                                         cATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTAttt                                                                         <  1:1088461/150‑1 (MQ=255)
                                                                         cATAGTCGTAATCATTTTCTTACCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCTCAATCGGCGGGAATAATATTCAGGTGTCCGGTTTCACAAATATAAAAAATGATCTATTCACTCTCTTGCTTAttt                                                                         <  2:1651406/150‑1 (MQ=255)
                                                                          aTCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTa                                                                        >  1:216941/1‑150 (MQ=255)
                                                                             gtcgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTg                                                                     <  1:572301/150‑1 (MQ=255)
                                                                                 tcatcaTTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACaa                                                                 >  1:938521/1‑150 (MQ=255)
                                                                                 tcatcaTTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACaa                                                                 >  2:1055513/1‑150 (MQ=255)
                                                                                 tcatcaTTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACaa                                                                 >  2:1287548/1‑150 (MQ=255)
                                                                                  catcttTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAAc                                                                <  1:177634/150‑1 (MQ=255)
                                                                                  catcatTTTCATCCCTTTACCCGCAATCAACGCAGTGTACTGCACCGTTTGCCGCTTGTCCTTGCACTATCGGCGGGAAAAATATTCAGGTGACCGGATTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAAc                                                                <  2:727785/150‑1 (MQ=255)
                                                                                    tcatTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTAAGGTGACAGGTTTCACAAATATAAAAAATGAACAATTCACTCTATTGCTTATTTAGTGACAACTa                                                              >  1:1506585/1‑150 (MQ=255)
                                                                                      atTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTAtt                                                            >  2:115481/1‑150 (MQ=255)
                                                                                       ttttCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTc                                                           <  2:1026733/150‑1 (MQ=255)
                                                                                            aTCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGAt                                                      >  1:1582377/1‑150 (MQ=255)
                                                                                             tCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGAtt                                                     >  2:1282293/1‑150 (MQ=255)
                                                                                              cccTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTTGTACAACTATTCAATAtttt                                                   >  2:1300734/1‑150 (MQ=255)
                                                                                                  ttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgtg                                                <  2:213854/150‑1 (MQ=255)
                                                                                                     cacGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTTTGaaa                                             >  2:281041/1‑150 (MQ=255)
                                                                                                     cacGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGaaa                                             >  2:1045977/1‑150 (MQ=255)
                                                                                                      acGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAc                                            >  1:1162406/1‑150 (MQ=255)
                                                                                                       cGCAATCAACGCAGTGTACTGCACCGTTTGCCGTTTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTGACTCTGTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAcc                                           <  2:1296298/150‑1 (MQ=255)
                                                                                                       cGCAATCAACGAAGTGTACTGCACCTTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGAACGGGTTCACAAATATAAAAAATGAACAATTCACTCTATTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAcc                                           <  2:727032/150‑1 (MQ=255)
                                                                                                            tCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGttt                                      <  2:1666365/150‑1 (MQ=255)
                                                                                                             cAACGCAGTGTACTGCACCGTTTGTCGATTGTCCTTGCACAATCGGCGGGAAATATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                     <  1:1674105/150‑1 (MQ=255)
                                                                                                             cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                     >  2:671754/1‑150 (MQ=255)
                                                                                                             cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                     >  1:1623319/1‑150 (MQ=255)
                                                                                                              aaCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCt                                    >  2:328748/1‑150 (MQ=255)
                                                                                                               aCGCAGTGTAATGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCtt                                   <  2:870731/150‑1 (MQ=255)
                                                                                                                  cAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAAt                                <  2:647052/150‑1 (MQ=255)
                                                                                                                    gtgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAAGAATTCCCTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTc                              >  2:1454275/1‑150 (MQ=255)
                                                                                                                     tgtACTGCACCGTTTGCCTATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTcc                             <  1:623645/150‑1 (MQ=255)
                                                                                                                      gtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACTAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCAGGATTTTGTGAAACCGGTTTCTTAATTCCg                            <  1:283497/150‑1 (MQ=255)
                                                                                                                         cTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGATACCGGTTACTTAATTCCCttt                         >  2:849654/1‑150 (MQ=255)
                                                                                                                         cTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGttt                         >  1:556025/1‑150 (MQ=255)
                                                                                                                           gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCa                       >  2:498902/1‑150 (MQ=255)
                                                                                                                           gCACCGTTTGCCGATTGTACTTGAACAATCGGCGGGAAAAATATTCATGTGACCGGTTTCACAAATATAAAAAATTAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCa                       <  1:959248/150‑1 (MQ=255)
                                                                                                                              ccGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCa                    >  2:1322886/1‑150 (MQ=255)
                                                                                                                                gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACTACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATc                  <  2:422845/150‑1 (MQ=255)
                                                                                                                                gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTAATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATc                  >  1:807499/1‑150 (MQ=255)
                                                                                                                                   cgCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGc               >  2:1249453/2‑150 (MQ=255)
                                                                                                                                    gCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa              >  1:407148/1‑150 (MQ=255)
                                                                                                                                      cGATTGTGCAAGGACAATCGGCATCAAAAAAATTAAGGTGACCTAATTAAAAAATATAAAAAATGAACAATTAACTCTCATTATAATTTAGATACAAAAATTCATGATTTTGTGAAACCGGTTTCTTAATTACTTTTCAGCATCGGCAtt            >  2:198661/1‑150 (MQ=255)
                                                                                                                                      cGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCAtt            >  1:381024/1‑150 (MQ=255)
                                                                                                                                        aTTGTCCTTGCACAATCGGCTGGAAAAATATTAAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCAtttt          <  2:91582/150‑1 (MQ=255)
                                                                                                                                            tCCTTGCACAATCGGCGGGAAAAATATTCAGTTGACCTGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg      <  1:470786/150‑1 (MQ=255)
                                                                                                                                            tCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg      <  1:1282293/150‑1 (MQ=255)
                                                                                                                                            tCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg      <  1:1296586/150‑1 (MQ=255)
                                                                                                                                                tGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCAc  >  2:133101/1‑150 (MQ=255)
                                                                                                                                                tGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCAc  <  1:943258/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
TGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCAC  >  NC_000913/2839180‑2839473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: