Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,863,783 T→C 12.0% V64A (GTC→GCC)  ygbL → putative class II aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,863,7830TC12.0% 254.6 / 22.5 100V64A (GTC→GCC) ygbLputative class II aldolase
Reads supporting (aligned to +/- strand):  ref base T (36/52);  new base C (5/7);  total (41/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.48e-01

Only 100 of 101 total aligned reads displayed.

ATGACGCGGATTGCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGG  >  NC_000913/2863635‑2863932
                                                                                                                                                    |                                                                                                                                                     
aTGACGCGGATTGCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTc                                                                                                                                                      <  2:586362/150‑1 (MQ=255)
 tGACGCGGATTGCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTcg                                                                                                                                                     <  2:46122/150‑1 (MQ=255)
  gACGCGGATTGCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTcgc                                                                                                                                                    >  1:1731933/1‑150 (MQ=255)
  gACGCGGATTGCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTcgc                                                                                                                                                    >  1:878836/1‑150 (MQ=255)
     gcgGATTGCCAGTTCATTCTTTCAGCTCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGAAACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTcgccgc                                                                                                                                                 <  2:821073/150‑1 (MQ=255)
     gcgGATTCCCAGTTCCTTCTTGCAGCGCGGCTATGCACCCGGCGCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGCGAATTTACTGGCGACACCGACAGGGTCATGCCTGGGCAATCTCGATCCGCGGCGACTTTGGAAAGCcgctgc                                                                                                                                                 <  1:272301/150‑1 (MQ=255)
      cgGATTGCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCcgcg                                                                                                                                                <  1:878874/150‑1 (MQ=255)
      cgGATTGCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGACTTTGGAAAGCCGCtgcg                                                                                                                                                <  2:46433/150‑1 (MQ=255)
         aTTGCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCGGCGACTTTGGAAAGCCGCTGCGGAt                                                                                                                                             <  1:967134/150‑1 (MQ=255)
         aTTGCCAGTTCATGCTTTCAGCGCGGCTATGCAACCGGCTCGGCTGGCAATCTGTCGCTGCTTTTCCCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCGGCGACTTTGGAAAGCCGCTGCGGAt                                                                                                                                             <  1:87761/150‑1 (MQ=255)
          ttGCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATg                                                                                                                                            <  1:1127592/150‑1 (MQ=255)
          ttGCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATg                                                                                                                                            <  1:468866/150‑1 (MQ=255)
           tGCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCGGCGACTTTGGAAAGCCGCTGCGGAtcg                                                                                                                                           <  2:629288/150‑3 (MQ=255)
            gCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGc                                                                                                                                          >  2:1033196/1‑150 (MQ=255)
            gCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGc                                                                                                                                          >  1:1250820/1‑150 (MQ=255)
             ccAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCg                                                                                                                                         >  2:175582/1‑150 (MQ=255)
                  gcatccattCAGCACGGCTATCCAACGGGATCGGCTGGCAATCTGTCGCTTCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGATCAAGCCTGGGCAATCTAGATCCGGAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATgg                                                                                                                                    <  2:1705535/143‑1 (MQ=255)
                   cATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGt                                                                                                                                   >  1:1567068/1‑150 (MQ=255)
                     ttCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTa                                                                                                                                 <  2:438070/150‑1 (MQ=255)
                       cTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAg                                                                                                                               >  1:1024518/1‑150 (MQ=255)
                       cTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAg                                                                                                                               >  1:1374005/1‑150 (MQ=255)
                       cTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAg                                                                                                                               >  2:627748/1‑150 (MQ=255)
                       cTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAg                                                                                                                               >  2:67300/1‑150 (MQ=255)
                         ttCAGCGCGGCTATGCAACCGGTTCTGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAgtg                                                                                                                             <  2:11218/150‑1 (MQ=255)
                         ttCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGGCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAgtg                                                                                                                             <  1:1519285/150‑1 (MQ=255)
                             gccgggCTATGCAACAGGTTCGGCTGGCAATCTGTCGCTGCTTTAACCTGACGGGAATATACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGa                                                                                                                         <  2:169156/146‑1 (MQ=255)
                              cgcgGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGAc                                                                                                                        <  2:410465/150‑1 (MQ=255)
                               gcgGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACa                                                                                                                       <  1:844514/150‑1 (MQ=255)
                                  gCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAAc                                                                                                                    >  2:1508626/1‑150 (MQ=255)
                                    tATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGCCACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAAccc                                                                                                                  >  2:1440480/1‑150 (MQ=255)
                                      tGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTc                                                                                                                <  2:70898/150‑1 (MQ=255)
                                       gCAACCGGTTCGTCTGGCAATCGGGCGCTGCGTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCg                                                                                                               <  2:1079995/150‑1 (MQ=255)
                                       gCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCg                                                                                                               >  1:1306380/1‑150 (MQ=255)
                                       gCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCg                                                                                                               <  2:1468289/150‑1 (MQ=255)
                                       gCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCg                                                                                                               >  2:1678159/1‑150 (MQ=255)
                                        cAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGa                                                                                                              >  2:366742/1‑150 (MQ=255)
                                         aaCCGTTTCTGCTGGCAATCTGTCGCTGCTTTTACCTGACGTGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGACGCGGATGGCGAATGGTTAAGTGGTGACAATCACTCGaa                                                                                                             <  2:386144/150‑1 (MQ=255)
                                          aCCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGaaa                                                                                                            <  1:1102247/150‑1 (MQ=255)
                                            cGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAaga                                                                                                          <  1:175582/150‑1 (MQ=255)
                                             ggTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAagag                                                                                                         >  2:805384/1‑150 (MQ=255)
                                              gTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAgg                                                                                                        <  2:606382/150‑1 (MQ=255)
                                                     gggCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATTCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCtt                                                                                                 <  2:1082186/149‑1 (MQ=255)
                                                      ggCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGCCGCCGCGGATGGCGAATGGTTAAGTGGCGACAAACCCTCGAAAGAGGTGCTCttt                                                                                                <  2:159744/150‑1 (MQ=255)
                                                      ggCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCAGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGAAGGCGAATGGTTAAGTGGTGACACACCCTCGAAAGAGGtccacatt                                                                                                >  2:251518/1‑143 (MQ=255)
                                                        cAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCa                                                                                              >  1:644525/1‑150 (MQ=255)
                                                        cAATCTGGCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCa                                                                                              <  1:1633785/150‑1 (MQ=255)
                                                          aTCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCAAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATc                                                                                            <  2:577903/150‑1 (MQ=255)
                                                           tCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGCACCTCTTTcgagg                                                                                           <  2:631820/150‑5 (MQ=255)
                                                             tGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTgg                                                                                         <  2:1154491/150‑1 (MQ=255)
                                                              gTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGgc                                                                                        <  2:352795/150‑1 (MQ=255)
                                                                 gctgctTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCt                                                                                     >  2:163951/1‑150 (MQ=255)
                                                                  ctgctTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTg                                                                                    <  1:170314/150‑1 (MQ=255)
                                                                  ctgctTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTg                                                                                    <  2:1291577/150‑1 (MQ=255)
                                                                       tttACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCg                                                                               >  1:776525/1‑150 (MQ=255)
                                                                       tttACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCg                                                                               <  2:1374005/150‑1 (MQ=255)
                                                                        ttACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGc                                                                              >  1:308730/1‑150 (MQ=255)
                                                                           ccTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGcaac                                                                           <  2:1351764/150‑1 (MQ=255)
                                                                              gACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAAt                                                                        <  1:1703954/150‑1 (MQ=255)
                                                                                cGGGAATTTACTGGCGACACCGTCAGGTTCATGCCTGGGCAATCTCGCTCCTCAGCGGCTATCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGGGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATcc                                                                      <  2:1629180/150‑1 (MQ=255)
                                                                                  ggAATTTACTGGCTACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCcgc                                                                    <  2:90352/150‑1 (MQ=255)
                                                                                   gAATTTACTCGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCGTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCcgcg                                                                   <  2:1067605/150‑1 (MQ=255)
                                                                                       ttACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGCTCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGAGAATGGTTACGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGt                                                               <  1:67300/150‑1 (MQ=255)
                                                                                         aCTGGCGCCACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGtta                                                             >  2:1090388/1‑148 (MQ=255)
                                                                                          cTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTaaa                                                            >  1:731217/1‑150 (MQ=255)
                                                                                          cTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTaaa                                                            >  1:724893/1‑150 (MQ=255)
                                                                                            ggCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGc                                                          <  1:753961/150‑1 (MQ=255)
                                                                                                 cacCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCggtgg                                                     <  2:1250820/150‑1 (MQ=255)
                                                                                                 cacCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCggtgg                                                     <  1:17383/150‑1 (MQ=255)
                                                                                                   ccGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGgtggtg                                                   <  1:347915/150‑1 (MQ=255)
                                                                                                     gACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCa                                                 <  2:521116/150‑1 (MQ=255)
                                                                                                       cAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCAtt                                               >  2:1209573/1‑150 (MQ=255)
                                                                                                       cAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCAtt                                               <  2:480733/150‑1 (MQ=255)
                                                                                                       cAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCAtt                                               <  2:1100437/150‑1 (MQ=255)
                                                                                                         ggTTCATGCCTGTGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGATAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTg                                             <  1:979336/150‑1 (MQ=255)
                                                                                                             cATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgcaca                                         <  1:1387046/150‑1 (MQ=255)
                                                                                                             cATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGAGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgcaca                                         >  2:933746/1‑150 (MQ=255)
                                                                                                               tggcTGGGCAATCTCGATCCGCAGCGGCTTTCCATAGTCGCCGGGGATGGCGTTTGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTGATATGGCGCTTTTTAGAAAGAATCCGCGCTGTAATGGTGTGTTGCATTTgcaaagt                                       >  1:110764/4‑149 (MQ=255)
                                                                                                               tGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgcacagc                                       <  2:324052/150‑1 (MQ=255)
                                                                                                                 ccTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgcacagcac                                     <  1:1131966/150‑1 (MQ=255)
                                                                                                                  cTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgcacagcaca                                    >  2:272406/1‑150 (MQ=255)
                                                                                                                     ggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATgg                                 <  1:1033196/150‑1 (MQ=255)
                                                                                                                     ggCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATgg                                 <  1:1440480/150‑1 (MQ=255)
                                                                                                                        aaTCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCg                              >  1:1319760/1‑150 (MQ=255)
                                                                                                                         ttcgccaTCCGCGGCGACTTTGGAAAGCCGCTGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCACCAATCCGCGCTGTAAAGCGGTGGTGCTTTTGACCAGGACATGGTCGa                             >  2:1137960/7‑150 (MQ=37)
                                                                                                                          tctcGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGAc                            <  1:1508626/150‑1 (MQ=255)
                                                                                                                           cgccaTCCGCGGCGACTTTGGAAAGCCGCTGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACg                           >  1:935518/5‑150 (MQ=39)
                                                                                                                           cgccaTCCGCGGCGACTTTGGAAAGCCGCTGCGGATCGCGAATGGTTAAGTGGTGACAAACCCACGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACg                           <  2:928367/146‑1 (MQ=38)
                                                                                                                            gccaTCCGCGGCGACTTTGGAAAGCCGCTGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGCCgg                          >  1:160556/4‑150 (MQ=255)
                                                                                                                             ctaTCCTCAGCGGCTTTCCATAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCTCTGTATCGCAACTATCCGAGCTGTAAAGCGGTGGTGCATTTGCACAGCACATTGTCGACGgc                         <  2:392885/148‑1 (MQ=255)
                                                                                                                             cGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGgc                         >  2:48864/1‑150 (MQ=255)
                                                                                                                             cGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGgc                         >  2:1529150/1‑150 (MQ=255)
                                                                                                                             cGAACCGCAGCGGCGTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGgc                         <  1:1153779/150‑1 (MQ=255)
                                                                                                                              caTCCGCGGCGACTTTGGAAAGCCGCTGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGgcg                        >  1:812336/2‑150 (MQ=255)
                                                                                                                               aTCCGCGGCGACTTTGGAAAGCCGCTGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCAACTGGAGCTGTATCGCAACAATCAGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGgcgc                       >  2:200848/1‑150 (MQ=255)
                                                                                                                                 ccGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCtt                     <  1:968720/150‑1 (MQ=255)
                                                                                                                                   gCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGATTGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATGCGCGCTGTATAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTc                   >  1:198938/1‑150 (MQ=255)
                                                                                                                                   gCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTc                   >  2:1636209/1‑150 (MQ=255)
                                                                                                                                              ccAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAg        >  1:1610495/1‑150 (MQ=255)
                                                                                                                                                   gTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGTCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCATGCAAGGGCTg   >  1:208399/1‑150 (MQ=255)
                                                                                                                                                    tCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAACGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTgg  <  2:1040349/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATGACGCGGATTGCCAGTTCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGG  >  NC_000913/2863635‑2863932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: