Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 324,579 A→G 5.5% intergenic (+126/+117) ykgG → / ← ykgH LutC family protein; putative electron transport chain YkgEFG component/putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913324,5790AG 5.5% 375.6 / 9.0 126intergenic (+126/+117)ykgG/ykgHLutC family protein; putative electron transport chain YkgEFG component/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (75/44);  new base G (5/2);  total (80/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.84e-01

Only 100 of 129 total aligned reads displayed.

ATCTGATTATTGAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTAC  >  NC_000913/324431‑324728
                                                                                                                                                    |                                                                                                                                                      
aTCTGATTATTGAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTAc                                                                                                                                                       >  1:748493/1‑150 (MQ=255)
  cTGATTATTGAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCa                                                                                                                                                     >  2:1668489/1‑150 (MQ=255)
   tGATTATTGAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATTCTGCTCTTCTGTAAATATCCTGGTATTACCaa                                                                                                                                                    >  1:836399/1‑150 (MQ=255)
   tGATTATTGAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCaa                                                                                                                                                    >  1:587535/1‑150 (MQ=255)
   tGATTATTGAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTCGTATTACCaa                                                                                                                                                    >  1:369875/1‑150 (MQ=255)
   tGATTATTGAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGAAAACCTGACTTAACACCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACaaa                                                                                                                                                    >  1:27094/1‑150 (MQ=255)
        attGAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTCACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCa                                                                                                                                               >  2:652663/1‑150 (MQ=255)
           gAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGa                                                                                                                                            >  2:599267/1‑150 (MQ=255)
           gAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGa                                                                                                                                            >  1:881538/1‑150 (MQ=255)
           gAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGCATTGGTAATACCAGGa                                                                                                                                            <  1:283392/150‑1 (MQ=255)
           gAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGATGAAAATATCGTTTAGGACAAAATGACTTAAACCCGTTCCATCATGGTTATGCTGCTCATCTGTAAATAAACTGGTATTACCAATGCCAGGa                                                                                                                                            >  1:115612/1‑150 (MQ=255)
             ggATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGatat                                                                                                                                         >  2:1640823/1‑150 (MQ=255)
                ttgttgAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATAttt                                                                                                                                       <  2:612263/150‑1 (MQ=255)
                       gagagaTATGAAGTCTGGACATAAACCTGGAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGa                                                                                                                                <  1:715099/150‑1 (MQ=255)
                         gagaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGaaa                                                                                                                              >  1:742912/1‑150 (MQ=255)
                         gagaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGaaa                                                                                                                              <  1:345603/150‑1 (MQ=255)
                           gaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATTTTTACAGTGaaaaa                                                                                                                            >  1:1323370/1‑150 (MQ=255)
                           gaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGaaaaa                                                                                                                            >  2:1456110/1‑150 (MQ=255)
                           gaTATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTACCGTCATGGTTATGCTGCTCTTCTGTAAATATACTGGTATTACCAATGCCAGGATATTTACAGTGaaaaa                                                                                                                            >  2:763794/1‑150 (MQ=255)
                             tatGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGa                                                                                                                          >  1:1732606/1‑150 (MQ=255)
                             tatGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATACTTGTATTACCAATGCCAGGATATTTACAGTGAAAAAGa                                                                                                                          >  1:831081/1‑150 (MQ=255)
                                gAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAg                                                                                                                       <  2:1646027/150‑1 (MQ=255)
                                 aaGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAgg                                                                                                                      <  1:1198374/150‑1 (MQ=255)
                                     cTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATc                                                                                                                  >  1:1718440/1‑150 (MQ=255)
                                     cTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATc                                                                                                                  >  2:1156625/1‑150 (MQ=255)
                                       ggACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCa                                                                                                                >  2:732182/1‑150 (MQ=255)
                                        gACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCAt                                                                                                               <  2:1650586/150‑1 (MQ=255)
                                             aaaCCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATTCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTg                                                                                                          >  2:426185/1‑150 (MQ=255)
                                             aaaCCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCATGGTATTACCAATGCCAGGTTATTTACAGTGAAAAAGACAGGCTTCCATTCCTg                                                                                                          >  2:857446/1‑150 (MQ=255)
                                               aCCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGaa                                                                                                        >  2:980243/1‑150 (MQ=255)
                                               aCCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGaa                                                                                                        >  2:532185/1‑150 (MQ=255)
                                                ccTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGaaa                                                                                                       >  2:844010/1‑150 (MQ=255)
                                                 cTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGaaaa                                                                                                      >  2:632473/1‑150 (MQ=255)
                                                   gaagaaGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCATGAAAACg                                                                                                    >  2:431634/1‑150 (MQ=255)
                                                     agaagGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACggg                                                                                                  >  2:757978/1‑150 (MQ=255)
                                                        agGCATACGATGTTGAAAATATCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttc                                                                                              <  1:1711423/150‑1 (MQ=255)
                                                        agGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttc                                                                                              <  2:1438672/150‑1 (MQ=255)
                                                        agGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttc                                                                                              >  1:658609/1‑150 (MQ=255)
                                                        agGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttcc                                                                                             <  1:1640823/150‑1 (MQ=255)
                                                         ggCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGttcc                                                                                             <  2:1613195/150‑1 (MQ=255)
                                                          gCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAAAGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGATAACGGGttccc                                                                                            >  1:306242/1‑150 (MQ=255)
                                                             tACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGCCAGGCATCCATTCCTGAAAACGGGttcccttc                                                                                         <  1:720494/150‑1 (MQ=255)
                                                                     tGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCAt                                                                                 >  1:749305/1‑150 (MQ=255)
                                                                        aaaTAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGt                                                                              <  1:599267/150‑1 (MQ=255)
                                                                            aGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGTATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACt                                                                          <  2:1657749/150‑1 (MQ=255)
                                                                                 ttAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGc                                                                     <  2:1224871/150‑1 (MQ=255)
                                                                                   aGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCtt                                                                   >  1:155437/1‑150 (MQ=255)
                                                                                   aGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCAGCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCtt                                                                   >  2:921399/1‑150 (MQ=255)
                                                                                    ggACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCttt                                                                  >  1:985715/1‑150 (MQ=255)
                                                                                    ggACAACCTGACTTAACCCCGTGCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACTGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCttt                                                                  >  1:906579/1‑150 (MQ=255)
                                                                                      aCAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTtat                                                                >  2:904561/1‑150 (MQ=255)
                                                                                      aCAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTtat                                                                >  2:1330435/1‑150 (MQ=255)
                                                                                       cAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTtata                                                               <  2:587535/150‑1 (MQ=255)
                                                                                         aCCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACa                                                             >  1:1258624/1‑150 (MQ=255)
                                                                                         aCCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCGTTATACa                                                             >  2:1518899/1‑150 (MQ=255)
                                                                                            tGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGc                                                          <  1:1471439/150‑1 (MQ=255)
                                                                                            tGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATAACCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGc                                                          >  2:1548488/1‑150 (MQ=255)
                                                                                            tGACTTAACCCCGTTACGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGc                                                          >  1:918105/1‑150 (MQ=255)
                                                                                              aCTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCAc                                                        <  1:980243/150‑1 (MQ=255)
                                                                                              aCTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCAc                                                        <  2:1388768/150‑1 (MQ=255)
                                                                                               cTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCAct                                                       >  2:1197371/1‑150 (MQ=255)
                                                                                               cTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCAct                                                       <  1:757978/150‑1 (MQ=255)
                                                                                                  aaCCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCActctc                                                    >  2:723225/1‑150 (MQ=255)
                                                                                                  aaCCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAAAGGGTTCCCTTACCGGTCATTGTCACTTCAGCTTTATACAGGCActcta                                                    >  2:1532276/1‑149 (MQ=255)
                                                                                                  aaCCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACAAATGCCAGGATATTTACAGTGAAAAAGACAGGAATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTTTCACTTCAGCTTTATACAGGCActcta                                                    >  2:1265155/1‑149 (MQ=255)
                                                                                                    ccccGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTa                                                  <  2:1162273/150‑1 (MQ=255)
                                                                                                      ccGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATc                                                <  1:1658128/150‑1 (MQ=255)
                                                                                                      ccGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATc                                                <  1:381007/150‑1 (MQ=255)
                                                                                                       cGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCa                                               <  2:1342300/150‑1 (MQ=255)
                                                                                                       cGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCa                                               >  1:1097296/1‑150 (MQ=255)
                                                                                                             gTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACATGCATCCATTCCTGAAAACGGGTTCACTTCCCGGTCATTGTCACTTCAGCTTTATACATACACTCTCTATCAGAAtgt                                         >  2:666940/1‑150 (MQ=255)
                                                                                                              tCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAAtgt                                         >  2:561806/1‑150 (MQ=255)
                                                                                                                aTGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAAtgttgt                                      <  1:1339454/150‑1 (MQ=255)
                                                                                                                 tGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATgttgtt                                     >  2:808645/1‑150 (MQ=255)
                                                                                                                    ttATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTttat                                  <  1:558548/150‑1 (MQ=255)
                                                                                                                       tgctgcTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTAc                               <  1:1251467/150‑1 (MQ=255)
                                                                                                                          tgctCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACTGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGACACTTCAGCTTTATACAGGCACTCACTATCAGAATGTTGTTTATTACTGc                            >  2:1707789/1‑150 (MQ=255)
                                                                                                                          tgctCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGc                            >  1:1445319/1‑150 (MQ=255)
                                                                                                                           gctCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGcc                           <  1:1460059/150‑1 (MQ=255)
                                                                                                                              cttctGTAAATATCCTGGTATTGCCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCag                        <  2:366279/150‑1 (MQ=255)
                                                                                                                                tctGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCagag                      >  1:1119803/1‑150 (MQ=255)
                                                                                                                                actGTAAATATCCTGGCATTGGTAATACCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCagag                      >  2:367859/2‑150 (MQ=39)
                                                                                                                                  tGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGtt                    >  1:1021614/1‑150 (MQ=255)
                                                                                                                                   gTAAATATCCTGGCATTGGTAATACCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGttt                   >  1:464897/1‑150 (MQ=39)
                                                                                                                                   gTAAATATCCTGGCATTGGTAATACCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGATGTTTATTACTGCCCAGAGttt                   >  2:506795/1‑150 (MQ=39)
                                                                                                                                     aaaTATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTttg                 >  2:821051/1‑150 (MQ=255)
                                                                                                                                     aaaTATCCTGGCATTGGTAATACCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATCCAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTttg                 >  2:871146/1‑150 (MQ=39)
                                                                                                                                     aaaTATCCTGGCATTGGTAATACCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTttg                 >  1:82336/1‑150 (MQ=39)
                                                                                                                                        tatCCTGGTATTACCAATGCCAGGATATTTACAGTGAAATAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTttgttg              <  1:652663/150‑1 (MQ=255)
                                                                                                                                        tatCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTttgttg              >  1:1281938/1‑150 (MQ=255)
                                                                                                                                          tCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGt            >  1:655766/1‑150 (MQ=255)
                                                                                                                                            cTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGCCAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTAt          >  1:860801/1‑150 (MQ=255)
                                                                                                                                            cTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTAt          >  2:66134/1‑150 (MQ=255)
                                                                                                                                             tGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTAtt         <  1:1379017/150‑1 (MQ=255)
                                                                                                                                               gTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCa       <  2:155437/150‑1 (MQ=255)
                                                                                                                                                tATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCAt      <  1:562911/150‑1 (MQ=255)
                                                                                                                                                 aTTACCAATGCCAGGATATTTACAGTGAAAAAGACAGCCATCCATAAATGAAAACGGGTTCAATTCCCGGTCATTTTAACTTCAGCTTTATACAGGCACTCGCGATCAGAATGTTGTTTATTACTGCCCAAATTTTTGTTGGTATTAAta     >  1:696163/1‑149 (MQ=255)
                                                                                                                                                   tACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTa   <  1:904561/150‑1 (MQ=255)
                                                                                                                                                    aCCAATGCCAGGATATTTACAGTGAAAAAGACAGGCCTCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTAc  <  1:1532276/150‑1 (MQ=255)
                                                                                                                                                    aCCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTAc  <  2:27094/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
ATCTGATTATTGAGGATTGTTGAGAGAGATATGAAGTCTGGACATAAACCTGAAGAAGGCATACGAGGTTGAAAATAGCGTTTAGGACAACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTAC  >  NC_000913/324431‑324728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: