Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,866,488 G→C 5.3% intergenic (+23/+71) ygbN → / ← rpoS putative transporter/RNA polymerase, sigma S (sigma 38) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,866,4880GC 5.3% 421.7 / 8.8 128intergenic (+23/+71)ygbN/rpoSputative transporter/RNA polymerase, sigma S (sigma 38) factor
Reads supporting (aligned to +/- strand):  ref base G (61/60);  new base C (2/5);  total (63/65)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

GTTCTGGATTGTGACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGA  >  NC_000913/2866339‑2866637
                                                                                                                                                     |                                                                                                                                                     
gTTCTGGATTGTGACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATggg                                                                                                                                                       <  1:660986/150‑1 (MQ=255)
gTTCTGGATTGTGACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATggg                                                                                                                                                       <  1:1436880/150‑1 (MQ=255)
gTTCTGGATTGTGACCAAATATCTGGGGTTGTCGGTAGCAGACGGGCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATggg                                                                                                                                                       <  2:399432/150‑1 (MQ=255)
  tCTGGATTGTGACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCa                                                                                                                                                     >  1:582342/1‑150 (MQ=255)
     ggATTGTGACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAAGTCCGATGGGCATCg                                                                                                                                                  <  2:1217773/150‑1 (MQ=255)
       aTTGTGACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCCGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGa                                                                                                                                                >  1:547993/1‑150 (MQ=255)
         tgtgACCAATTATCTGGGGTTGTCGGTAGCTGACGGTCTGTAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATATAAAGGTCCGATGGGCATCGGAcc                                                                                                                                              <  1:356708/150‑1 (MQ=255)
         tgtgACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGCCCATCGGAcc                                                                                                                                              <  1:337298/150‑1 (MQ=255)
          ttgACCTAATATCTGGGGTTGGCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGCCCATCGGACCt                                                                                                                                             <  1:836689/149‑1 (MQ=255)
            gACCAAATATCTGGGGTTTTCGGTTGGAGACGGTCTGAAAACGTTGACAGTGTTAACGACCATTCTCGGTTTTACATGATTCTTTATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGCCCATCGGACCttt                                                                                                                                           <  2:280338/150‑1 (MQ=255)
            gACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCttt                                                                                                                                           <  2:1703719/150‑1 (MQ=255)
             aCCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGATAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCtttt                                                                                                                                          <  1:1053373/150‑1 (MQ=255)
              ccAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGCCCATCGGACCttttt                                                                                                                                         <  1:681715/150‑2 (MQ=255)
               cAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTAt                                                                                                                                        >  2:922604/1‑150 (MQ=255)
               cAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTAt                                                                                                                                        >  2:916557/1‑150 (MQ=255)
                aaaTATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGCCCATCGGACCtttttat                                                                                                                                       <  1:1601748/150‑4 (MQ=255)
                 aaTATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTAttt                                                                                                                                      >  2:1336604/1‑149 (MQ=255)
                    atCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGc                                                                                                                                   <  1:712357/150‑1 (MQ=255)
                     tCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACTGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGca                                                                                                                                  >  2:1152332/1‑150 (MQ=255)
                                cGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGcc                                                                                                                       <  1:1521276/150‑1 (MQ=255)
                                cGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGcc                                                                                                                       <  2:1158331/150‑1 (MQ=255)
                                 ggTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCa                                                                                                                      >  1:384788/1‑150 (MQ=255)
                                  ggtgCAGACGGTCTGCTCACGTGGACTGTGTTTACGACGATTCTCGGGTTTTGCGGATTCTTATTTACCTGGTGCGTATGGGCGGTAATTTGTCCTTAATAAAAAGGTCGGATGGGCATCGGACCTTTTATTGTGCACTGAAAAGGCCAg                                                                                                                     <  1:929879/147‑1 (MQ=255)
                                    aGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGcc                                                                                                                   >  2:1199150/1‑150 (MQ=255)
                                    aGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGcc                                                                                                                   <  2:1002996/150‑1 (MQ=255)
                                           ggTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTg                                                                                                            <  2:844923/150‑1 (MQ=255)
                                           ggTCAGAAAACCAGGACAGTGTTAACGACCCTTCTCGGTTTTACCGGATTCTTACTTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGGCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTg                                                                                                            <  2:328997/150‑1 (MQ=255)
                                            ctCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTga                                                                                                           >  1:325682/2‑150 (MQ=255)
                                                gAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACt                                                                                                       <  1:699217/150‑1 (MQ=255)
                                                gAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACt                                                                                                       >  2:1616698/1‑150 (MQ=255)
                                                gAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACt                                                                                                       <  2:1732707/150‑1 (MQ=255)
                                                gAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACt                                                                                                       >  2:84493/1‑150 (MQ=255)
                                                   aaCCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGc                                                                                                    >  2:1642866/1‑150 (MQ=255)
                                                   aaCCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCCTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGc                                                                                                    <  1:679366/150‑1 (MQ=255)
                                                    aCCTGGACATTGTTAACGACCATTCTCGGGTTTACCGGATTCTTTATTACCTGGTGCGTTTGGGCGGTAATTTGACCTTAATAAAAAGGGCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGcc                                                                                                   <  2:48153/150‑1 (MQ=255)
                                                    aCCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGcc                                                                                                   <  2:564662/150‑1 (MQ=255)
                                                    aCCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCATAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCAGCGGACCTTTATTGTGCACAGAAAAGGCCAGTCTCAAGCGAGGCTGGCCt                                                                                                  <  2:341342/150‑1 (MQ=255)
                                                      cTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCtt                                                                                                 >  2:721292/1‑150 (MQ=255)
                                                       tGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGTCTCAAGCGAGGCTGGCCttt                                                                                                <  1:1498667/150‑1 (MQ=255)
                                                       tGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCttt                                                                                                >  1:36448/1‑150 (MQ=255)
                                                        ggACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTc                                                                                               >  2:1256069/1‑150 (MQ=255)
                                                         gACAGTGTTAACGACCATTCTCGGTTTTACCGTATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGTCTCAAGCGAGGCTGGCCttttc                                                                                              <  2:562900/150‑3 (MQ=255)
                                                         gACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCt                                                                                              >  2:141555/1‑150 (MQ=255)
                                                         gACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCt                                                                                              <  1:499729/150‑1 (MQ=255)
                                                         gACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCATAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCt                                                                                              <  2:1362335/150‑1 (MQ=255)
                                                            aGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGAc                                                                                           <  1:916557/150‑1 (MQ=255)
                                                            aGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGAc                                                                                           <  1:922604/150‑1 (MQ=255)
                                                             gtgtTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAACGGCCCGCCTCGCGTGAGACTGGCCTTTCTGACa                                                                                          >  2:268229/1‑150 (MQ=255)
                                                               gtTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGCCAGa                                                                                        >  2:334814/1‑150 (MQ=255)
                                                                 tAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATg                                                                                      >  1:1299179/1‑150 (MQ=255)
                                                                    cGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGctt                                                                                   <  2:714479/150‑1 (MQ=255)
                                                                      aCCATTCTCGGTTTTACCGGATTCTTAATTTCCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGcttac                                                                                 <  2:1134580/150‑1 (MQ=255)
                                                                       ccATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTTTGCACAGAAAATGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGattact                                                                                >  2:832602/1‑150 (MQ=255)
                                                                        cATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCAGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGcttactt                                                                               >  1:952559/1‑150 (MQ=255)
                                                                          ttCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCttacttac                                                                             <  1:129703/150‑1 (MQ=255)
                                                                            ctcGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGCCCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTc                                                                           >  2:908129/1‑150 (MQ=255)
                                                                            ctcGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTc                                                                           >  1:1604582/1‑150 (MQ=255)
                                                                              cGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTcgc                                                                         <  1:125651/150‑1 (MQ=255)
                                                                               ggTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTcgcg                                                                        <  1:588148/150‑1 (MQ=255)
                                                                               ggTTTTACAGGATTCTTTATTATCTGGTGAGTATGGGAGGTTATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGAACTTTTATTGTGCACAGATTTGGCCAGCCTCGCTTGAGTCTGGCCTTTCTGACAGATGCTTAGTTACTcgag                                                                        <  2:1415941/150‑3 (MQ=255)
                                                                                gTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTAGCgg                                                                       >  1:753027/1‑150 (MQ=255)
                                                                                           ttCTTAATTACCTGGTGCGTATTGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTc                                                            <  1:512526/150‑1 (MQ=255)
                                                                                           ttCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTc                                                            >  2:494216/1‑150 (MQ=255)
                                                                                           ttCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTc                                                            >  2:145823/1‑150 (MQ=255)
                                                                                            tCTTAATTACCTGGTGCGTCTGGGCGGTAATTTGACCTAAATAAAAAGGTCCGATGTGCATCGGACCTTTTATTGTGCTCAGAAAACGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACATCGCTTCg                                                           <  2:177431/150‑1 (MQ=255)
                                                                                            tCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCGGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCg                                                           <  2:1663249/150‑1 (MQ=255)
                                                                                            tCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCg                                                           <  2:1260383/150‑1 (MQ=255)
                                                                                              ttAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGat                                                         >  1:857266/1‑150 (MQ=255)
                                                                                              ttAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGat                                                         >  2:244352/1‑150 (MQ=255)
                                                                                                  ttACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTc                                                     <  1:1727150/150‑1 (MQ=255)
                                                                                                     ccTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGc                                                  >  1:1423573/1‑150 (MQ=255)
                                                                                                        ggTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAACGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGcccc                                               <  1:612697/150‑1 (MQ=255)
                                                                                                              tATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGGTGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGtt                                         <  1:906045/150‑1 (MQ=255)
                                                                                                               ttGGGCGGTAATTTGACCTTAATAACAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGttt                                        <  2:1332777/149‑1 (MQ=255)
                                                                                                                  ggCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCa                                     >  1:1486538/1‑150 (MQ=255)
                                                                                                                   gCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAg                                    <  2:13237/150‑1 (MQ=255)
                                                                                                                    cGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAgg                                   >  1:1005443/1‑150 (MQ=255)
                                                                                                                    cGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAgg                                   >  2:1040624/1‑150 (MQ=255)
                                                                                                                      gTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGAt                                 >  2:899541/1‑150 (MQ=255)
                                                                                                                         aTTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCgg                                                                       <  2:1391705/109‑1 (MQ=255)
                                                                                                                         aTTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCgg                                                                       >  1:1391705/1‑109 (MQ=255)
                                                                                                                         aTTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCGTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGAGTGGCCTTTATGACAGATGCTTACTTTCTCGCGGAAGAGCGCTTCGATATTCAGCACCTGCGTTTGCAGGAttta                              >  2:863063/1‑149 (MQ=255)
                                                                                                                          tttGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTcg                             <  2:1620654/150‑1 (MQ=255)
                                                                                                                              aCCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCATGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCAGCGTTTGCAGGATTTCGCGCa                         >  2:1487196/1‑150 (MQ=255)
                                                                                                                                   aataaAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCAAAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTAACTTACACGCGGAACAGCGCTTCGATATTCAGCCCCTGCGATTGCAGGATTTAGCGCAAACgg                    >  2:417206/1‑150 (MQ=255)
                                                                                                                                    ataaAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGgc                   >  2:397259/1‑150 (MQ=255)
                                                                                                                                      aaaaaGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTACGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGgcgc                 >  2:811754/1‑150 (MQ=255)
                                                                                                                                         aaGGTCCGATGGGCAGCGGGCGTGGTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCACTGCGTTTGAAGGATTTCGCGCAAACGCCGCAgg              >  2:1253077/1‑150 (MQ=255)
                                                                                                                                           ggTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGcc            >  2:135547/1‑150 (MQ=255)
                                                                                                                                            gTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCt           <  2:1483927/150‑1 (MQ=255)
                                                                                                                                            gTCCGATGCCCATCGGACCTTTTATTGTGCACAGAAAAGGCAAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCt           >  1:267242/1‑150 (MQ=255)
                                                                                                                                              ccGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCACGGAACAGCGCTTCGATATTAAGCCCCTGCGTATTCAGGATTTCGCGCAAACGGCGCAGGCCtta         >  2:157581/1‑149 (MQ=255)
                                                                                                                                                gATGGGAATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCaa       <  2:708276/150‑1 (MQ=255)
                                                                                                                                                  tGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAcc     <  2:547993/150‑1 (MQ=255)
                                                                                                                                                  tGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAcc     <  1:1156813/150‑1 (MQ=255)
                                                                                                                                                   gggCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCCGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGTCTTCAACCt    >  1:195792/1‑150 (MQ=255)
                                                                                                                                                    ggCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTg   >  2:517700/1‑150 (MQ=255)
                                                                                                                                                    ggCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTg   >  1:506092/1‑150 (MQ=255)
                                                                                                                                                    ggCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTAGCGCAAACGGCGCAGGCCTTCAACCTg   >  2:102944/1‑150 (MQ=255)
                                                                                                                                                     gCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGa  <  2:952559/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTCTGGATTGTGACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGA  >  NC_000913/2866339‑2866637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: