Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,877,564 T→C 6.1% R328R (CGT→CGC) ‡ iap → aminopeptidase in alkaline phosphatase isozyme conversion

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,877,5640TC 6.1% 373.8 / 11.4 131R328R (CGT→CGC) ‡iapaminopeptidase in alkaline phosphatase isozyme conversion
Reads supporting (aligned to +/- strand):  ref base T (72/50);  new base C (2/6);  total (74/57)
Fisher's exact test for biased strand distribution p-value = 7.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.23e-02

Only 100 of 131 total aligned reads displayed.

AACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGTTTATCCC  >  NC_000913/2877418‑2877711
                                                                                                                                                  |                                                                                                                                                   
aaCTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGCCTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGAc                                                                                                                                                  >  2:1054742/1‑150 (MQ=255)
  cTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCACCATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCCTGACGt                                                                                                                                                >  1:262117/1‑150 (MQ=255)
  cTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGt                                                                                                                                                >  1:675059/1‑150 (MQ=255)
  cTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGt                                                                                                                                                >  1:1216134/1‑150 (MQ=255)
  cTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGt                                                                                                                                                >  2:598427/1‑150 (MQ=255)
  cTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGt                                                                                                                                                >  1:252825/1‑150 (MQ=255)
   tGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGtt                                                                                                                                               <  2:538215/150‑1 (MQ=255)
    ggAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTa                                                                                                                                              >  2:12538/1‑150 (MQ=255)
    ggAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCACGGCAGCGACGttc                                                                                                                                              <  1:1161405/150‑2 (MQ=255)
    ggAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCACGGCAGCGACGttc                                                                                                                                              <  1:110262/150‑2 (MQ=255)
      aaTCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCAGGAAGAATAGAACGTCGCTGCCGTGACGTTATg                                                                                                                                            <  2:1221618/150‑1 (MQ=255)
       aTCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCACGGCAGCGACGttctat                                                                                                                                           <  1:1724929/150‑5 (MQ=255)
         cTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCTTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCgg                                                                                                                                         >  2:919625/1‑150 (MQ=255)
          ttGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGa                                                                                                                                        >  1:821006/1‑150 (MQ=255)
           tGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATACTCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTACCGTGACGTTATGCGGAt                                                                                                                                       <  2:1038865/150‑1 (MQ=255)
             ggAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATaa                                                                                                                                     >  1:2335/1‑150 (MQ=255)
              gAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAAt                                                                                                                                    >  1:1470202/1‑150 (MQ=255)
                 tAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCt                                                                                                                                 <  1:388057/150‑1 (MQ=255)
                   aGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGTTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTAc                                                                                                                               >  2:537570/1‑150 (MQ=255)
                       tGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACctct                                                                                                                           >  1:971031/1‑150 (MQ=255)
                       tGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACctct                                                                                                                           >  1:724268/1‑150 (MQ=255)
                           tATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGCCGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTg                                                                                                                       >  2:266768/1‑150 (MQ=255)
                           tATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTg                                                                                                                       >  2:1082327/1‑150 (MQ=255)
                           tATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTg                                                                                                                       >  2:1645141/1‑150 (MQ=255)
                           tATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTg                                                                                                                       <  2:784255/150‑1 (MQ=255)
                           tATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTg                                                                                                                       <  2:951634/150‑1 (MQ=255)
                             tCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGaa                                                                                                                     >  2:100457/1‑150 (MQ=255)
                              cAGCAACGCTCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGAATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTTGTGAAg                                                                                                                    <  1:630116/150‑1 (MQ=255)
                              cAGCAACGCGCAAAAACACCTTCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAg                                                                                                                    <  1:960834/150‑1 (MQ=255)
                               aGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAgg                                                                                                                   >  1:1449983/1‑150 (MQ=255)
                                gCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGa                                                                                                                  >  2:1343393/1‑150 (MQ=255)
                                gCAACGAGCAAAAACACCTGCCTTCCCGGCGGGAAATATCTTGCATGACGTAAGACTGGATAATCACCAACATATTGATAATGCTCATCCTGGAAGAATAGAACGTCGCTGCCGTGAAGATATGCGGATAATGCTAACTCTGGTGAAGGa                                                                                                                  >  1:357250/1‑150 (MQ=255)
                                   aCGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGtt                                                                                                               <  1:1150422/150‑1 (MQ=255)
                                   aCGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGtt                                                                                                               <  2:635676/150‑1 (MQ=255)
                                    cgcgCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTg                                                                                                              >  2:827731/1‑150 (MQ=255)
                                     gcgcAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTgg                                                                                                             <  1:1501821/150‑1 (MQ=255)
                                     gcgcAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTgg                                                                                                             <  2:750316/150‑1 (MQ=255)
                                          aaaaCACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAg                                                                                                        >  2:353061/1‑150 (MQ=255)
                                          aaaaCACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAg                                                                                                        >  1:515427/1‑150 (MQ=255)
                                           aaaCACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAgg                                                                                                       <  1:1661085/150‑1 (MQ=255)
                                               acCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTc                                                                                                   <  2:1666593/150‑1 (MQ=255)
                                                  tGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTg                                                                                                >  1:1320441/1‑150 (MQ=255)
                                                   gCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGa                                                                                               >  2:387000/1‑150 (MQ=255)
                                                      ttCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATgg                                                                                            <  2:1351681/150‑1 (MQ=255)
                                                       tCCCGGCGGGATATAGCTGGCATGACGTTAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATggg                                                                                           <  1:383859/150‑1 (MQ=255)
                                                        cccGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGt                                                                                          >  1:181502/1‑150 (MQ=255)
                                                          cggcggGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATCTTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGttt                                                                                        <  2:832544/150‑1 (MQ=255)
                                                          cggcggGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGttt                                                                                        <  1:51100/150‑1 (MQ=255)
                                                           ggcggGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTg                                                                                       <  2:10992/150‑1 (MQ=255)
                                                              gggAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGaaa                                                                                    >  1:1469787/1‑150 (MQ=255)
                                                                     aGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAg                                                                             <  1:1150978/150‑1 (MQ=255)
                                                                      gCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGTATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGc                                                                            >  2:287360/1‑150 (MQ=255)
                                                                            aTGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAg                                                                      <  1:100457/150‑1 (MQ=255)
                                                                            aTGACGTAAGACTGCATAATCACCAACATATTGATAAGGCTCTTCCTCGAAGAATAGAACGTAGCTGCCCTGACGATATGCGGATAATGCTACCTCTGTAGAAGGAGTTGGCGACGGCGACTTCATGGGTTTGAAAAACGGAGCTggcaa                                                                      >  1:306047/1‑147 (MQ=255)
                                                                             tGACGTAAGACTGGATAAACACCAACATATTGATAAGGCTCTTCCAGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGt                                                                     <  2:607919/150‑1 (MQ=255)
                                                                              gACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGtt                                                                    >  1:570754/1‑150 (MQ=255)
                                                                              gACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTGGATGGGTTTGAAAATGGGAGCTGGGAGtt                                                                    >  1:674210/1‑150 (MQ=255)
                                                                                  tAAGACTGGATAATCACCATCATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGAGGGGTTTGAAAATGGGAGCTGGGAGTTCTAc                                                                <  1:266768/150‑1 (MQ=255)
                                                                                   aaGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTAcc                                                               >  2:1113721/1‑150 (MQ=255)
                                                                                    aGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCg                                                              >  2:1514838/1‑150 (MQ=255)
                                                                                        tGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCaga                                                          >  1:1042004/1‑150 (MQ=255)
                                                                                         ggATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCagag                                                         >  2:606528/1‑150 (MQ=255)
                                                                                         ggATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCagag                                                         >  2:902420/1‑150 (MQ=255)
                                                                                           aTAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATTATGCTACCTCTGGTGAAGGAGTTGGCGAAGTCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGAAGAGGc                                                       >  2:198511/1‑150 (MQ=255)
                                                                                            tAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCg                                                      >  1:332859/1‑150 (MQ=255)
                                                                                              aTCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCggg                                                    <  1:606528/150‑1 (MQ=255)
                                                                                              aTCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCggg                                                    >  2:223377/1‑150 (MQ=255)
                                                                                              aTCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGCGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCggg                                                    <  1:920713/150‑1 (MQ=255)
                                                                                               tCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCgggg                                                   <  2:252825/150‑1 (MQ=255)
                                                                                                cACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCggggg                                                  >  2:1605653/1‑150 (MQ=255)
                                                                                                     aCATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTc                                             <  2:524198/150‑1 (MQ=255)
                                                                                                      cATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTcc                                            <  1:1645141/150‑1 (MQ=255)
                                                                                                         atTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCTTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCAAAg                                         >  2:1222707/1‑150 (MQ=255)
                                                                                                           tGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTg                                       >  2:366084/1‑150 (MQ=255)
                                                                                                                  gCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCca                                <  2:1470202/150‑1 (MQ=255)
                                                                                                                   ctctTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCcat                               >  2:543375/1‑150 (MQ=255)
                                                                                                                    tctTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCcatc                              <  2:147626/150‑1 (MQ=255)
                                                                                                                        ccTGGACGAATAGAACGTCGCTGCCGTGACGGTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGGCTTGATGGGTTTGAAAAGGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCg                          <  1:1416017/150‑1 (MQ=255)
                                                                                                                         cTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGc                         >  1:1629804/1‑150 (MQ=255)
                                                                                                                             aagaaTAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATcc                     <  1:1341637/150‑1 (MQ=255)
                                                                                                                             aagaaTAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATcc                     <  1:827731/150‑1 (MQ=255)
                                                                                                                                aaTAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGt                  >  1:1562259/1‑150 (MQ=255)
                                                                                                                                aaTAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGt                  >  2:254478/1‑150 (MQ=255)
                                                                                                                                  cataaCGTCACGGCAGCGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCCTCGCCTCCAGTgc                >  2:318885/4‑150 (MQ=255)
                                                                                                                                  cataaCGTCACGGCAGCGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTgc                >  1:372248/4‑150 (MQ=255)
                                                                                                                                    gAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCggggg                                                  >  1:1023491/1‑114 (MQ=255)
                                                                                                                                    gAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCggggg                                                  <  2:1023491/114‑1 (MQ=255)
                                                                                                                                    gAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTgcgc              >  1:509531/1‑150 (MQ=255)
                                                                                                                                         tCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGt         <  1:1565841/150‑1 (MQ=255)
                                                                                                                                         tCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGt         <  2:1576299/150‑1 (MQ=255)
                                                                                                                                         tCGCTGCCGGGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGt         <  2:46976/150‑1 (MQ=255)
                                                                                                                                          ggCTGCCGTGACGTTATGCGGATAATGCTTCCTCTGGTGAAGGAGTAGGCGAAGGCGTCTTGATGGGTTTGATTATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGtt        <  1:161068/149‑1 (MQ=255)
                                                                                                                                           gCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGttt       >  1:1249429/1‑150 (MQ=255)
                                                                                                                                              gCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGGCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGTTTATc    <  2:821006/150‑1 (MQ=255)
                                                                                                                                              gCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGGCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGTTTATc    <  1:12538/150‑1 (MQ=255)
                                                                                                                                               ccGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGCAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGTTTATcc   >  1:686498/1‑150 (MQ=255)
                                                                                                                                               ccGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGTTTATcc   >  2:191476/1‑150 (MQ=255)
                                                                                                                                                cGTGACGTTATGCGGATAATGCTTCCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGTTTATccc  <  1:1440234/150‑1 (MQ=255)
                                                                                                                                                cGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGTTTATccc  >  1:849119/1‑150 (MQ=255)
                                                                                                                                                cGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGTTTATccc  >  2:1326847/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATGACGTAAGACTGGATAATCACCAACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGTTTATCCC  >  NC_000913/2877418‑2877711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑