Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 324,874 T→G 5.7% N164T (AAC→ACC) ‡ ykgH ← putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913324,8740TG 5.7% 398.3 / 9.8 137N164T (AAC→ACC) ‡ykgHputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (55/73);  new base G (5/3);  total (60/77)
Fisher's exact test for biased strand distribution p-value = 3.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

Only 100 of 139 total aligned reads displayed.

TACCATTTTTTTGACAAGGCAAAACATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTCAGC  >  NC_000913/324726‑325022
                                                                                                                                                    |                                                                                                                                                    
tACCATTTTTTTGACAAGGCAAAACATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGTTACAATGACCAGtt                                                                                                                                                     >  1:213779/1‑150 (MQ=255)
 aCCATTTTTTTGACAAGGCAAAACATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTc                                                                                                                                                    >  2:102412/1‑150 (MQ=255)
    aTTTTTTTGACAAGGCAAAACATTACAGAAATTACTATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCtt                                                                                                                                                 >  1:885638/1‑149 (MQ=255)
    aTTTTTTTGACAAGGCAAAACATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTg                                                                                                                                                 >  1:468409/1‑150 (MQ=255)
    aTTTTTTTGACAAGGCAAAACATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTg                                                                                                                                                 <  2:660723/150‑1 (MQ=255)
      ttttttGACAAGGCAAAACATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGt                                                                                                                                               >  1:1129759/1‑150 (MQ=255)
       tttttGACAAGGCAAAACATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTc                                                                                                                                              >  1:305761/1‑150 (MQ=255)
          ttGACAAGGCAAAACATTACAGAAATTACAATGCTAAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGGAACTGGTCatt                                                                                                                                           <  2:306242/150‑3 (MQ=255)
           tGACAAGGCAAAACATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGt                                                                                                                                          >  1:1068446/1‑150 (MQ=255)
           tGACAAGGCAAAACATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGGAACTGGTCattg                                                                                                                                          <  2:748493/150‑4 (MQ=255)
            gACAAGGCAAAACATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAACTGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGta                                                                                                                                         <  1:118147/150‑1 (MQ=255)
            gACAAGGCAAAACATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGta                                                                                                                                         >  2:454492/1‑150 (MQ=255)
                 ggCAAAACATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTatataa                                                                                                                                    >  1:1542756/1‑148 (MQ=255)
                  gCAAAACATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCAt                                                                                                                                   <  1:1445925/150‑1 (MQ=255)
                  gCAAAACATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCAt                                                                                                                                   >  1:1725036/1‑150 (MQ=255)
                  gCAAAACATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCAt                                                                                                                                   >  2:595002/1‑150 (MQ=255)
                    aaaaCATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCAttt                                                                                                                                 <  1:941627/150‑1 (MQ=255)
                      aaCATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCAttttt                                                                                                                               <  2:464897/150‑1 (MQ=255)
                       aCATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTg                                                                                                                              <  2:1495171/150‑1 (MQ=255)
                        cATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGt                                                                                                                             <  2:906776/150‑1 (MQ=255)
                        cATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTACGGATACAATGACCAGTTACTGGTCAGGTATATCATTTTTGt                                                                                                                             >  2:1094367/1‑150 (MQ=255)
                         aTTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCATGTAATTTATTTTTTATTTCTGGTGAGCTTATAGATACACAGCCATCACCGTTCGGATACAATGACCAGTTACATGTAAGGTATATCATTATTGta                                                                                                                            >  1:168752/1‑150 (MQ=255)
                          ttACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGtat                                                                                                                           >  1:1519395/1‑150 (MQ=255)
                          ttACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGtat                                                                                                                           <  2:83237/150‑1 (MQ=255)
                          ttACAGAAATTACAATGCTTAGAAAAAATCCAAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGcat                                                                                                                           <  2:1501324/150‑1 (MQ=255)
                            aCAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATaa                                                                                                                         <  1:506795/150‑1 (MQ=255)
                             cAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGCGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATaaa                                                                                                                        >  2:41753/1‑150 (MQ=255)
                               gAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCATGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGTTCAGGTATAGCATTTTTGTATAAAgg                                                                                                                      >  1:273527/1‑149 (MQ=255)
                               gAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGc                                                                                                                      >  1:1561820/1‑150 (MQ=255)
                                    tACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACt                                                                                                                 <  1:1330435/150‑1 (MQ=255)
                                    tACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGAGACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACt                                                                                                                 <  1:668474/150‑1 (MQ=255)
                                       aaTGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGtt                                                                                                              <  1:903750/150‑1 (MQ=255)
                                       aaTGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGtt                                                                                                              <  2:62561/150‑1 (MQ=255)
                                           cTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAAtt                                                                                                          <  1:1456110/150‑1 (MQ=255)
                                           cTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAAtt                                                                                                          >  2:183808/1‑150 (MQ=255)
                                             tAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGTGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGc                                                                                                        <  1:1427215/150‑1 (MQ=255)
                                             tAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGc                                                                                                        <  2:650721/150‑1 (MQ=255)
                                              aGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCt                                                                                                       <  1:871146/150‑1 (MQ=255)
                                               gAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTg                                                                                                      <  1:723225/150‑1 (MQ=255)
                                                aaaaaaTCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGa                                                                                                     >  1:362409/1‑150 (MQ=255)
                                                        ccAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGttt                                                                                             <  2:1443439/150‑1 (MQ=255)
                                                        ccAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGttt                                                                                             >  1:920836/1‑150 (MQ=255)
                                                         cAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTg                                                                                            <  2:528305/150‑1 (MQ=255)
                                                          agagCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGt                                                                                           <  2:1431573/150‑1 (MQ=255)
                                                              ccAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTAtct                                                                                       <  1:391406/150‑1 (MQ=255)
                                                              ccAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTAtct                                                                                       <  2:1647232/150‑1 (MQ=255)
                                                               cAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGCTACAATGACCCGTTCCTGGGCAGGTATATCATTTATGTAGAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTAtctc                                                                                      >  1:1064689/1‑150 (MQ=255)
                                                                aGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTTATTGCTGAAGGCGTTTGTATCTCa                                                                                     >  2:1504600/1‑150 (MQ=255)
                                                                aGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCa                                                                                     >  1:905989/1‑150 (MQ=255)
                                                                aGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGATTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCATGTATATAATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTAtctaa                                                                                     >  1:1499775/1‑148 (MQ=255)
                                                                 ggCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCAt                                                                                    >  1:956250/1‑150 (MQ=255)
                                                                   cAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCg                                                                                  <  2:278202/150‑1 (MQ=255)
                                                                   cAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCg                                                                                  >  1:672458/1‑150 (MQ=255)
                                                                   cAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGATCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCg                                                                                  <  1:1440320/150‑1 (MQ=255)
                                                                   cAAATGGCAGCAGGGTAATTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCg                                                                                  <  2:1415952/150‑1 (MQ=255)
                                                                       tGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGaa                                                                              <  2:305761/150‑1 (MQ=255)
                                                                        ggCAGAAGGGTATTTATTTTTTATTTCTTGTGAGCTTATATTTACACAGCCATCACCGTTCGGATACAATGACCAGCACCTGGTCATTTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAAc                                                                             <  2:96947/150‑1 (MQ=255)
                                                                           agcagGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTtg                                                                          <  1:66134/150‑1 (MQ=255)
                                                                               gggTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTtgcctg                                                                      <  2:687265/150‑1 (MQ=255)
                                                                               gggTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTtgcctg                                                                      <  2:905989/150‑1 (MQ=255)
                                                                                  tatttattTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCa                                                                   <  1:948705/150‑1 (MQ=255)
                                                                                   atttatttTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCAt                                                                  <  2:1359721/150‑1 (MQ=255)
                                                                                    tttattttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCATGGTCAGGTATATCATTTTTGTATAAATCTCACTGTTAATTGCTGAAGGCGTTAGTATCTCATCTAGAAATTGACTGCCAtt                                                                 >  1:281456/1‑150 (MQ=255)
                                                                                     tttttttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGTTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTa                                                                <  1:715803/147‑1 (MQ=255)
                                                                                     ttattttttatttCTGGTGAGCTTTTTGTTTAACAGCGATCACCGTTCGGATACAATGTCCAGTTCCTGGTCTGGTATATCATTTTTGATTAAAGCTCACTGTTAATTGCTGTAGGTGTTTGTATCTCATCGAGAACTTGCCTGCCATTa                                                                <  1:1299871/150‑1 (MQ=255)
                                                                                          ttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGAACAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTGACTGTTAATTGCTGAAGGCGTTTGTATGTCATCGAGAAATTGCGTGCGATTATGatt                                                           >  1:960093/1‑149 (MQ=255)
                                                                                             tatttCTGGTGAGCTTATAGTTACACACCCATCACCGTTCGGATACAATGACCAGTACCTGGTCAGGTATATCATTTATGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACATGCCTGCCATTATGATATTc                                                        <  2:277639/150‑1 (MQ=255)
                                                                                              atttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCt                                                       <  2:1304993/150‑1 (MQ=255)
                                                                                                  cTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACg                                                   <  2:1129759/150‑1 (MQ=255)
                                                                                                   tGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGc                                                  <  2:468409/150‑1 (MQ=255)
                                                                                                      tGAGCTTATAGTTACACAGCCATCCCCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATa                                               >  2:455788/1‑150 (MQ=255)
                                                                                                      tGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGTTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTCAAGGCGATTGTATATCATCCAGAAATTGCCTGCCATTATGATATTCTGACGCATa                                               >  1:1044946/1‑150 (MQ=255)
                                                                                                         gCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCa                                            <  1:556926/150‑1 (MQ=255)
                                                                                                           ttATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCAtt                                          <  2:1561820/150‑1 (MQ=255)
                                                                                                            tataGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATTTCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCAttt                                         <  1:384925/150‑1 (MQ=255)
                                                                                                             ataGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTg                                        <  1:183808/150‑1 (MQ=255)
                                                                                                               aGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGat                                      <  2:790628/150‑1 (MQ=255)
                                                                                                                  tACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGataat                                   >  2:218046/1‑150 (MQ=255)
                                                                                                                       aGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCTCTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAgg                              <  1:1659502/150‑1 (MQ=255)
                                                                                                                          cATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATAGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCt                           >  2:251335/1‑150 (MQ=255)
                                                                                                                           aTCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTTTGATATTCTGACGCATACCATTTGATAATAGAGGCCTg                          <  1:789887/150‑1 (MQ=255)
                                                                                                                           aTCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTg                          <  2:1191433/150‑1 (MQ=255)
                                                                                                                             cACCGTTCGGATACAATGACCAGTTCGTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATGGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCa                        >  2:1232980/1‑150 (MQ=255)
                                                                                                                               ccGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCAcg                      <  1:1184561/150‑1 (MQ=255)
                                                                                                                               ccGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGGTTGTATCTCATCGAGATCTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCAcg                      <  1:184720/150‑1 (MQ=255)
                                                                                                                                cGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCAcgc                     >  1:827283/1‑150 (MQ=255)
                                                                                                                                   tCGGATTCAATGACCAGTTCCTGGTCTGGTATATCATTTTTGTATAAAGCTCACTTTTAATTGCTGAAGGCGTTTGTATGTCATTGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCgag                  <  1:639215/150‑1 (MQ=255)
                                                                                                                                       aTACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGatat              <  1:595002/150‑1 (MQ=255)
                                                                                                                                         tacctGACCAGGAACTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATAttt            >  1:935103/5‑150 (MQ=255)
                                                                                                                                         aCAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATAttt            <  2:956250/150‑1 (MQ=255)
                                                                                                                                           cctGACCAGGAACTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTcc          <  2:699757/148‑1 (MQ=255)
                                                                                                                                           cctGACCAGGAACTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTcc          >  2:385370/3‑150 (MQ=255)
                                                                                                                                            aTGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCct         >  1:1598794/1‑150 (MQ=255)
                                                                                                                                            aTGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCct         >  1:127652/1‑150 (MQ=255)
                                                                                                                                            aTGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCct         <  1:1257361/150‑1 (MQ=255)
                                                                                                                                            aTGACCAGTTCCTGGTCAGGTATATAATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCct         <  1:1707789/150‑1 (MQ=255)
                                                                                                                                             tGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCctc        <  2:797352/150‑1 (MQ=255)
                                                                                                                                                ccAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTc     >  1:1618383/1‑150 (MQ=255)
                                                                                                                                                ccAGTTCCTGGTCAGGTATATCATTTCTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTc     <  1:767378/150‑1 (MQ=255)
                                                                                                                                                   gTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTcagc  <  1:218046/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TACCATTTTTTTGACAAGGCAAAACATTACAGAAATTACAATGCTTAGAAAAAATCCCAGAGCCAGGCAAATGGCAGCAGGGTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAGAACTTGCCTGCCATTATGATATTCTGACGCATACCATTTGATAATAGAGGCCTGCACGCGAGATATTTCCTCTTCAGC  >  NC_000913/324726‑325022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: