Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,900,322 C→T 7.1% intergenic (‑49/‑270) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,900,3220CT 7.1% 455.8 / 15.1 153intergenic (‑49/‑270)ygcW/yqcEputative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (68/74);  new base T (7/4);  total (75/78)
Fisher's exact test for biased strand distribution p-value = 3.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-02

Only 100 of 157 total aligned reads displayed.

GCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACA  >  NC_000913/2900176‑2900470
                                                                                                                                                  |                                                                                                                                                    
gCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCcgc                                                                                                                                                   >  2:36901/1‑150 (MQ=255)
 acTGGCCTAAACCGCTATTCCCACCGGTATCAATTGCGGTTGTACCTTTCAGGGAGAAAAAATCCATTGAGAAAGCATTGAGAGATTCGATTGAAATAGTTAGCTCCAATTTCAGTAAGGCTAAATATAAGAAATCTAACTTCCCCcgcg                                                                                                                                                  >  2:1435469/2‑150 (MQ=255)
   tGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTACCCCGCgat                                                                                                                                                >  2:713838/1‑149 (MQ=255)
   tGGCCTAAACCGCTATTACCACCGGTAACAATTGCGGTTTTACATTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCgag                                                                                                                                                <  1:342746/150‑1 (MQ=255)
     gCCTAAACCGCTATTGCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTTAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGtgag                                                                                                                                              <  1:616553/150‑1 (MQ=255)
      ccTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTGCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAgg                                                                                                                                             >  1:1329909/1‑150 (MQ=255)
       cTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAggg                                                                                                                                            <  2:1404281/150‑1 (MQ=255)
         aaaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGaa                                                                                                                                          >  2:1249277/1‑150 (MQ=255)
          aaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAg                                                                                                                                         >  1:105081/1‑150 (MQ=255)
          aaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAg                                                                                                                                         >  1:190623/1‑150 (MQ=255)
              gCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTAc                                                                                                                                     <  1:453791/150‑1 (MQ=255)
              gCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAc                                                                                                                                     <  1:307188/150‑1 (MQ=255)
              gCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAc                                                                                                                                     <  1:1350224/150‑1 (MQ=255)
               cTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCTTTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAcc                                                                                                                                    <  2:1595062/150‑1 (MQ=255)
                tATTCCCACCGGTAACAATTTCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTACCt                                                                                                                                   <  1:1713141/150‑1 (MQ=39)
                    cccACCGGTAACACTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTACCTTtct                                                                                                                               <  1:1266865/150‑1 (MQ=39)
                     ccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTTCCTTtctc                                                                                                                              >  2:1195688/1‑150 (MQ=255)
                     ccACCGGTAAAATTTTGGGTTTTAACTTTGAGGGTTTAAAAATCCATATACTAACCATTGTTTTAATCGTTTTAAAAATTTATCTCCAATTTACGTAAAGCAAAAAAAAAGTATGGTTACTTCCCCCGCGAGAGGGAAGTTACCTTtctc                                                                                                                              <  2:806315/150‑1 (MQ=255)
                          ggTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAt                                                                                                                         <  2:243035/150‑1 (MQ=255)
                           gTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtctc                                                                                                                      <  2:410333/150‑1 (MQ=255)
                           gTAACAATTGCGGTTTTACCATTCAGGGAGAAAAAAACCAATGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCACTTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtc                                                                                                                        <  1:659488/150‑1 (MQ=255)
                            tAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtct                                                                                                                       <  2:49111/150‑1 (MQ=255)
                                aaTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAACGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTgg                                                                                                                   >  1:287662/1‑150 (MQ=255)
                                  ttGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTa                                                                                                                 <  2:710141/150‑1 (MQ=255)
                                   tGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTaa                                                                                                                >  1:172499/1‑150 (MQ=255)
                                     cGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACt                                                                                                              <  2:1498954/150‑1 (MQ=255)
                                      ggTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTAACCCGCGAGAGGGAACTTACCTTTCTCATTATCTCTGGTAACTg                                                                                                             >  2:77878/1‑150 (MQ=255)
                                       gTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTgg                                                                                                            <  1:268263/150‑1 (MQ=255)
                                       gTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCAAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAAATTCCCCCGCGAGAGGGAAGTTACCTTTCTCAATATCGCTGGTAACTgg                                                                                                            >  2:1223092/1‑150 (MQ=255)
                                        ttttACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGga                                                                                                           <  1:1453832/150‑1 (MQ=255)
                                         tttACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGgag                                                                                                          >  2:1065012/1‑150 (MQ=255)
                                         tttACCTATCAGGGAGAATAAAAGGAATCACTAAGCATTGAGAGAGTCGCTAGACTTAGCTCGCTCCAATTTCCGTAAGGCATTAAAATAGACAGGCTACTTGCGCCGCCAGAGGGAAGTTACCTTTGTCTTTATCTCTGGTAACTGgag                                                                                                          <  1:1345006/150‑1 (MQ=255)
                                           tACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCCAGTTACCAGAGATa                                                                                                       <  1:1454947/150‑1 (MQ=255)
                                            aCCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCCAGTTACCAGAGATa                                                                                                       <  1:1676075/150‑1 (MQ=255)
                                              cTTTCATGGAGAAAAAATCCATTGATAAAGCATTGAGAGATTCGATTGACATATTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGTTAACTTCCCCCGCGAGAGTGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAt                                                                                                     <  1:141843/150‑1 (MQ=255)
                                                     ggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGc                                                                                              >  2:634301/1‑150 (MQ=255)
                                                     ggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTCACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTATCATTATCTCTGGTAACTGGAGTTAATTTTTAGc                                                                                              >  2:1177857/1‑150 (MQ=255)
                                                          aaaaaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATaat                                                                                         >  1:208804/1‑149 (MQ=255)
                                                            caacgcATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAggg                                                                                       <  1:73645/145‑1 (MQ=255)
                                                            aaaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAggg                                                                                       <  1:1686576/150‑1 (MQ=255)
                                                            aaaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAggg                                                                                       <  2:1118989/150‑1 (MQ=255)
                                                             aaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGc                                                                                      <  2:1213533/150‑1 (MQ=255)
                                                             aaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGc                                                                                      >  2:1438000/1‑150 (MQ=255)
                                                               tCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCAt                                                                                    >  1:794515/1‑150 (MQ=255)
                                                                 cATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATaa                                                                                  >  2:263770/1‑150 (MQ=255)
                                                                    tGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAATGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTg                                                                               <  2:1335110/150‑1 (MQ=255)
                                                                     gagaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGc                                                                              >  1:140789/1‑150 (MQ=255)
                                                                         aCGCATTGAGAGATTAGATTGACATTGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTACACCTCCAGAGGTAAGTTAACTTTCTCATTATCTATGGTAAATGGAGATAATTTTTAGCATAAGGGCATAACTTCTAAt                                                                          >  1:163665/1‑150 (MQ=255)
                                                                          cGCATTGAGTGATTCGATTGAAATGGTTATCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGATGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtg                                                                         <  1:1158531/150‑1 (MQ=255)
                                                                          cGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGGGAGGGAAGTTCCCTTTCTCATTATCTCTGGTAACTGGTGATAATTTTTAGCATAAGGGCATAACTGCTAAtg                                                                         >  2:980443/1‑150 (MQ=255)
                                                                            cATTGAGAGATTCGATTGACATAGTTAGCTCCATTTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtgtt                                                                       >  1:1656990/1‑149 (MQ=255)
                                                                            aaTTGAGAGATTCTATTTACATAGTTAGCTCAAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtgtg                                                                       <  2:1495633/149‑1 (MQ=255)
                                                                             aTTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGa                                                                      >  1:1240367/1‑150 (MQ=255)
                                                                             aTTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGAt                                                                     <  1:367116/150‑1 (MQ=255)
                                                                               tGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATATCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATc                                                                    >  1:697692/1‑150 (MQ=255)
                                                                                gagagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCg                                                                   <  2:472224/150‑1 (MQ=255)
                                                                                gagagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCg                                                                   <  2:1147212/150‑1 (MQ=255)
                                                                                gagagaTGCCATTGACATAGTTAGCTCCAAATTCCGTAAGGCAAAAAAAAAGAAACGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGCTAATTTTTAGCATAAGGGCATAACTGCTAATGTGAGCg                                                                   <  2:303898/150‑1 (MQ=255)
                                                                                 agagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGt                                                                  >  1:1297507/1‑150 (MQ=255)
                                                                                        cGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAACTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATcaca                                                           >  1:455228/1‑150 (MQ=255)
                                                                                          aTTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgt                                                         <  2:453314/150‑1 (MQ=255)
                                                                                            tGACATAGTTAGCTCCAATTTGCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgtgt                                                       <  2:1329909/150‑1 (MQ=255)
                                                                                              aCATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAAATTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATATCTGGTAACTGGAGATAATTTTTAGAATAAGTGAATAACTGCTAATGTGATCTTAATCACAGTGTGa                                                     >  2:1100176/1‑150 (MQ=255)
                                                                                               cATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGat                                                    >  1:624900/1‑150 (MQ=255)
                                                                                               cATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGata                                                   <  1:1547600/150‑1 (MQ=255)
                                                                                                 tAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGatat                                                  <  1:544094/150‑1 (MQ=255)
                                                                                                     tAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCTTAATCACAGTGTGATATTCGt                                              >  2:613278/1‑150 (MQ=255)
                                                                                                       gCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTAt                                            <  2:190623/150‑1 (MQ=255)
                                                                                                       cctaaaaTTTCCTTAAGGCAAAAAAATATAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTAt                                            <  2:1457355/145‑1 (MQ=255)
                                                                                                        cTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGtgtg                                                      >  1:1214618/1‑139 (MQ=255)
                                                                                                          ccAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGt                                         >  2:1730570/1‑150 (MQ=255)
                                                                                                          ccAATTTCCGTAAGGCAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTc                                        <  2:1470681/150‑1 (MQ=255)
                                                                                                           cAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGGGAGAGGGAAGTTACCATTCTCATTAACTCTGGTAACTGGAGATAATTTTTAGCATAAGGGAATAAATGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTc                                        >  2:295177/1‑150 (MQ=255)
                                                                                                           cAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTgac                                        >  2:722854/1‑148 (MQ=255)
                                                                                                           cAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTc                                        <  2:64694/150‑1 (MQ=255)
                                                                                                           cAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTc                                        <  1:990460/150‑1 (MQ=255)
                                                                                                            aaTTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCa                                       >  1:291348/1‑150 (MQ=255)
                                                                                                             aTTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCTTAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCaa                                      >  1:1325103/1‑150 (MQ=255)
                                                                                                                tCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAaata                                   <  2:312264/150‑1 (MQ=255)
                                                                                                                tCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAaata                                   >  2:100394/1‑150 (MQ=255)
                                                                                                                  cGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCGCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAaataat                                 >  1:1388125/1‑150 (MQ=255)
                                                                                                                   gTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAAtt                                <  2:1663910/150‑1 (MQ=255)
                                                                                                                       agCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaa                            >  1:630835/2‑150 (MQ=255)
                                                                                                                           aaaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTTGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAg                        <  1:1249277/150‑1 (MQ=255)
                                                                                                                             aaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCa                      >  2:726538/1‑150 (MQ=255)
                                                                                                                             aaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCa                      >  2:425511/1‑150 (MQ=255)
                                                                                                                             aaaaaaGAAAGGTAACTTCCCCCACGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCa                      >  2:893996/1‑150 (MQ=255)
                                                                                                                                  attatggTAACTTACCCCGCGAGAGGGAAGTTACCTTTCTCTTTATCTCTGTTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGc                 <  1:123886/145‑1 (MQ=255)
                                                                                                                                  agaaagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGc                 <  1:43905/150‑1 (MQ=255)
                                                                                                                                     aagGTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTg              >  1:316528/1‑150 (MQ=255)
                                                                                                                                     aagGTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTg              >  2:906288/1‑150 (MQ=255)
                                                                                                                                      agGTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGa             >  2:1658322/1‑150 (MQ=255)
                                                                                                                                       ggTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGAt            >  1:948078/1‑150 (MQ=255)
                                                                                                                                       ggTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGAt            >  1:556101/1‑150 (MQ=255)
                                                                                                                                            cTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTActc       <  2:740674/150‑1 (MQ=255)
                                                                                                                                            cTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTActc       <  1:1438000/150‑1 (MQ=255)
                                                                                                                                            cTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTActc       <  1:1431304/150‑1 (MQ=255)
                                                                                                                                                ccccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcac   <  1:1065012/150‑1 (MQ=255)
                                                                                                                                                 cccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGACAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcaca  <  1:722854/150‑1 (MQ=255)
                                                                                                                                                 cccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATAGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcaca  >  1:794413/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACA  >  NC_000913/2900176‑2900470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: