Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,900,327 A→C 7.3% intergenic (‑54/‑265) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,900,3270AC 7.3% 437.3 / 18.6 153intergenic (‑54/‑265)ygcW/yqcEputative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (65/74);  new base C (7/4);  total (74/79)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

Only 100 of 157 total aligned reads displayed.

TGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACA  >  NC_000913/2900179‑2900472
                                                                                                                                                     |                                                                                                                                                 
tGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTACCCCGCgat                                                                                                                                                  >  2:713838/1‑149 (MQ=255)
 aggtaaaaCCGCAATTGTTACCGGTGGGAATAGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGCACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAAGAAAGGTAACTTCCCCCGCgag                                                                                                                                                  >  2:1446108/6‑150 (MQ=37)
   ccTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTGCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAgg                                                                                                                                               >  1:1329909/1‑150 (MQ=255)
    cTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAggg                                                                                                                                              <  2:1404281/150‑1 (MQ=255)
      aaaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGaa                                                                                                                                            >  2:1249277/1‑150 (MQ=255)
       aaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAg                                                                                                                                           >  1:105081/1‑150 (MQ=255)
       aaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAg                                                                                                                                           >  1:190623/1‑150 (MQ=255)
           gCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAc                                                                                                                                       <  1:1350224/150‑1 (MQ=255)
             tATTCCCACCGGTAACAATTTCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTACCt                                                                                                                                     <  1:1713141/150‑1 (MQ=39)
              aTTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCtt                                                                                                                                    <  2:1013575/150‑1 (MQ=255)
                 cccACCGGTAACACTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTACCTTtct                                                                                                                                 <  1:1266865/150‑1 (MQ=39)
                 cccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtct                                                                                                                                 <  2:191935/150‑1 (MQ=255)
                  ccACCGGTAAAATTTTGGGTTTTAACTTTGAGGGTTTAAAAATCCATATACTAACCATTGTTTTAATCGTTTTAAAAATTTATCTCCAATTTACGTAAAGCAAAAAAAAAGTATGGTTACTTCCCCCGCGAGAGGGAAGTTACCTTtctc                                                                                                                                <  2:806315/150‑1 (MQ=255)
                       ggTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAt                                                                                                                           <  2:243035/150‑1 (MQ=255)
                        gTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtctc                                                                                                                        <  2:410333/150‑1 (MQ=255)
                             aaTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAACGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTgg                                                                                                                     >  1:287662/1‑150 (MQ=255)
                               ttGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTa                                                                                                                   <  2:723013/150‑1 (MQ=255)
                                    gTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTgg                                                                                                              >  1:528402/1‑150 (MQ=255)
                                     ttttACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGga                                                                                                             <  1:1453832/150‑1 (MQ=255)
                                      tttACCTATCAGGGAGAATAAAAGGAATCACTAAGCATTGAGAGAGTCGCTAGACTTAGCTCGCTCCAATTTCCGTAAGGCATTAAAATAGACAGGCTACTTGCGCCGCCAGAGGGAAGTTACCTTTGTCTTTATCTCTGGTAACTGgag                                                                                                            <  1:1345006/150‑1 (MQ=255)
                                        tACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCCAGTTACCAGAGATa                                                                                                         <  1:1454947/150‑1 (MQ=255)
                                         aCCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCCAGTTACCAGAGATa                                                                                                         <  1:1676075/150‑1 (MQ=255)
                                           cTTTCATGGAGAAAAAATCCATTGATAAAGCATTGAGAGATTCGATTGACATATTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGTTAACTTCCCCCGCGAGAGTGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAt                                                                                                       <  1:141843/150‑1 (MQ=255)
                                             ttCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAttt                                                                                                     <  2:756971/150‑1 (MQ=255)
                                             ttCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAttt                                                                                                     <  2:714729/150‑1 (MQ=255)
                                              tCAGGGAGAAAACATCCATTGAGAACGCATTGAGATATTCGATTGACATAGTTAGCTCCAATTTCCGTTAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCCAGTTACCAGAGATAAtgag                                                                                                    <  1:302124/150‑4 (MQ=255)
                                               cAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTACGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGTGAGGGAAGTTACCTTTCTCATTATCTATGGTAAATGGAGATAAttttt                                                                                                   >  2:1263024/1‑150 (MQ=255)
                                                 gcgagaTAAAATCCATTTATAACGCATTGAGAGATTCGATTGACATATTTTGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTTCCTTTCTAATTATCTCTGGTAACTGGAGATAATTTTTAg                                                                                                 <  2:476848/148‑1 (MQ=255)
                                                  ggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTCACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTATCATTATCTCTGGTAACTGGAGTTAATTTTTAGc                                                                                                >  2:1177857/1‑150 (MQ=255)
                                                   gagaAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCa                                                                                               >  1:740674/1‑150 (MQ=255)
                                                         caacgcATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAggg                                                                                         <  1:73645/145‑1 (MQ=255)
                                                          aaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGc                                                                                        >  2:1431304/1‑150 (MQ=255)
                                                          aaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGc                                                                                        <  2:1213533/150‑1 (MQ=255)
                                                            tCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCAt                                                                                      >  1:794515/1‑150 (MQ=255)
                                                              cATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATaa                                                                                    >  1:244729/1‑150 (MQ=255)
                                                                ttGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTg                                                                                 <  2:463895/150‑1 (MQ=255)
                                                                 tGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAATGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTg                                                                                 <  2:1335110/150‑1 (MQ=255)
                                                                  gagaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGc                                                                                >  1:1707414/1‑150 (MQ=255)
                                                                  gagaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGc                                                                                >  2:677595/1‑150 (MQ=255)
                                                                  gagaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGc                                                                                >  1:140789/1‑150 (MQ=255)
                                                                      aCGCATTGAGAGATTAGATTGACATTGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTACACCTCCAGAGGTAAGTTAACTTTCTCATTATCTATGGTAAATGGAGATAATTTTTAGCATAAGGGCATAACTTCTAAt                                                                            >  1:163665/1‑150 (MQ=255)
                                                                       cGCATTGAGTGATTCGATTGAAATGGTTATCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGATGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtg                                                                           <  1:1158531/150‑1 (MQ=255)
                                                                       cGCATTGAGAGATTCGATTGACATATTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAATGGCATAACTGCTAAtg                                                                           <  2:1182653/150‑1 (MQ=255)
                                                                       cGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGGGAGGGAAGTTCCCTTTCTCATTATCTCTGGTAACTGGTGATAATTTTTAGCATAAGGGCATAACTGCTAAtg                                                                           >  2:980443/1‑150 (MQ=255)
                                                                         cATTGAGAGATTCGATTGACATAGTTAGCTCCATTTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtgtt                                                                         >  1:1656990/1‑149 (MQ=255)
                                                                         aaTTGAGAGATTCTATTTACATAGTTAGCTCAAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtgtg                                                                         <  2:1495633/149‑1 (MQ=255)
                                                                          aTTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGa                                                                        >  1:1240367/1‑150 (MQ=255)
                                                                          aTTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGAt                                                                       <  1:367116/150‑1 (MQ=255)
                                                                            tGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATATCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATc                                                                      >  1:697692/1‑150 (MQ=255)
                                                                             gagagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCg                                                                     <  2:1147212/150‑1 (MQ=255)
                                                                             gagagaTGCCATTGACATAGTTAGCTCCAAATTCCGTAAGGCAAAAAAAAAGAAACGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGCTAATTTTTAGCATAAGGGCATAACTGCTAATGTGAGCg                                                                     <  2:303898/150‑1 (MQ=255)
                                                                              agagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGt                                                                    >  1:1297507/1‑150 (MQ=255)
                                                                                  aTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATc                                                                <  2:1574273/150‑1 (MQ=255)
                                                                                     cGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAACTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATcaca                                                             >  1:455228/1‑150 (MQ=255)
                                                                                       aTTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgt                                                           <  2:453314/150‑1 (MQ=255)
                                                                                       aTTGACATAGTTAGCTCAAATTTACTTAAGTCAAAAAAAAAGAAAGTTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgt                                                           <  2:415911/150‑1 (MQ=255)
                                                                                           aCATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAAATTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATATCTGGTAACTGGAGATAATTTTTAGAATAAGTGAATAACTGCTAATGTGATCTTAATCACAGTGTGa                                                       >  2:1100176/1‑150 (MQ=255)
                                                                                            cATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGat                                                      >  1:283148/1‑150 (MQ=255)
                                                                                            cATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGat                                                      <  1:179615/150‑1 (MQ=255)
                                                                                            cATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGat                                                      >  1:624900/1‑150 (MQ=255)
                                                                                            cATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCCTAATCACATTGTGat                                                      >  2:1604436/1‑150 (MQ=255)
                                                                                            cATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGata                                                     <  1:1547600/150‑1 (MQ=255)
                                                                                             atatTTAGCTCCAATTTCAGTAATTCAAAATAAAAGAAAGGTAACTTCCCCATCTAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGTAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGata                                                     <  1:333900/150‑1 (MQ=255)
                                                                                                  tAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCTTAATCACAGTGTGATATTCGt                                                >  2:613278/1‑150 (MQ=255)
                                                                                                    gCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTAt                                              >  2:1492101/1‑150 (MQ=255)
                                                                                                    cctaaaaTTTCCTTAAGGCAAAAAAATATAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTAt                                              <  2:1457355/145‑1 (MQ=255)
                                                                                                     cTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGtgtg                                                        <  2:1214618/139‑1 (MQ=255)
                                                                                                     cTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGtgtg                                                        >  1:1214618/1‑139 (MQ=255)
                                                                                                       ccAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGt                                           >  2:1730570/1‑150 (MQ=255)
                                                                                                        cAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGGGAGAGGGAAGTTACCATTCTCATTAACTCTGGTAACTGGAGATAATTTTTAGCATAAGGGAATAAATGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTc                                          >  2:295177/1‑150 (MQ=255)
                                                                                                        cAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTc                                          <  2:64694/150‑1 (MQ=255)
                                                                                                         aaTTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCa                                         >  2:631233/1‑150 (MQ=255)
                                                                                                         aaTTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCa                                         >  1:592795/1‑150 (MQ=255)
                                                                                                          aTTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCTTAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCaa                                        >  1:1325103/1‑150 (MQ=255)
                                                                                                             tCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAaata                                     >  2:100394/1‑150 (MQ=255)
                                                                                                             tCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAaata                                     <  2:312264/150‑1 (MQ=255)
                                                                                                               cGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCGCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAaataat                                   >  1:1388125/1‑150 (MQ=255)
                                                                                                                    agCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaa                              >  1:630835/2‑150 (MQ=255)
                                                                                                                         aaaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTTGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAg                          <  1:1249277/150‑1 (MQ=255)
                                                                                                                           aaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCa                        <  1:634301/150‑1 (MQ=255)
                                                                                                                           aaaaaaGAAAGGTAACTTCCCCCACGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCa                        >  2:893996/1‑150 (MQ=255)
                                                                                                                              aaagaaagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGaa                     >  1:449714/1‑150 (MQ=255)
                                                                                                                                attatggTAACTTACCCCGCGAGAGGGAAGTTACCTTTCTCTTTATCTCTGTTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGc                   <  1:123886/145‑1 (MQ=255)
                                                                                                                                agaaagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGc                   <  1:43905/150‑1 (MQ=255)
                                                                                                                                 gaaagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCg                  <  1:713838/150‑1 (MQ=255)
                                                                                                                                   aagGTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTg                >  2:906288/1‑150 (MQ=255)
                                                                                                                                   aagGTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTg                >  1:316528/1‑150 (MQ=255)
                                                                                                                                   aagGTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTg                >  2:169182/1‑150 (MQ=255)
                                                                                                                                    agGTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGa               >  2:1658322/1‑150 (MQ=255)
                                                                                                                                    agGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGa               >  2:111187/1‑150 (MQ=255)
                                                                                                                                     ggTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGAt              >  1:948078/1‑150 (MQ=255)
                                                                                                                                     ggTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGAt              >  1:556101/1‑150 (MQ=255)
                                                                                                                                          cTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTActc         <  1:1438000/150‑1 (MQ=255)
                                                                                                                                          cTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTActc         <  1:1431304/150‑1 (MQ=255)
                                                                                                                                          cTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTActc         <  2:740674/150‑1 (MQ=255)
                                                                                                                                             cccccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTca      <  1:978132/150‑1 (MQ=255)
                                                                                                                                              ccccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcac     <  1:1065012/150‑1 (MQ=255)
                                                                                                                                               ccggcgTGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGTGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcaca    <  1:295177/147‑1 (MQ=255)
                                                                                                                                                 cgcgAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcacaca  >  2:78605/1‑150 (MQ=255)
                                                                                                                                                 cgcgAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcacaca  >  1:1675384/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACA  >  NC_000913/2900179‑2900472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: