BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,907,998	A→T	7.4%	intergenic (‑57/+31)	eno ← / ← pyrG	enolase/CTP synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,907,998	0	A	T	7.4%	359.1 / 9.2	134	intergenic (‑57/+31)	eno/pyrG	enolase/CTP synthetase
Reads supporting (aligned to +/- strand): ref base A (66/54); new base T (3/8); total (69/65)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

AAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACG  >  NC_000913/2907850‑2908147

aaaCCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAg                                                                                                                                                      >  1:1518013/1‑150 (MQ=255)

  accaccCTCCAGTTGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAggg                                                                                                                                                    <  2:1548405/150‑1 (MQ=255)

  accaccCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAggg                                                                                                                                                    <  1:270638/150‑1 (MQ=255)

   ccaccCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGt                                                                                                                                                   >  2:646517/1‑150 (MQ=255)

       ccTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTAcgc                                                                                                                                               >  2:567878/1‑150 (MQ=255)

        cTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTAcgcg                                                                                                                                              >  2:581331/1‑150 (MQ=255)

          ccAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGtt                                                                                                                                            <  1:1647397/150‑1 (MQ=255)

          ccAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGAATTTTACGATTATGGACATTAGGTTTTCCTCAAGGCACTAGTTAAACTGTAACTCCAGACAAACAACGCGTACCCAGGGTACGCGtt                                                                                                                                            >  1:101870/1‑150 (MQ=255)

           cAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTg                                                                                                                                           >  1:337577/1‑150 (MQ=255)

             gATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTTAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGcc                                                                                                                                         >  2:557987/1‑150 (MQ=255)

             gATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTgtt                                                                                                                                         <  1:1696479/150‑3 (MQ=255)

               tGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGGCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGTTgtttg                                                                                                                                       <  1:387300/150‑5 (MQ=255)

                gTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGTTgtttgt                                                                                                                                      <  1:478427/150‑6 (MQ=255)

                gTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGTTgtttgt                                                                                                                                      <  1:1666102/150‑6 (MQ=255)

                 tACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGTTgtttgtc                                                                                                                                     <  1:646517/150‑7 (MQ=255)

                  agttCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGctct                                                                                                                                    <  2:474327/148‑1 (MQ=255)

                   cTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGAAGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTACTAAATTCAATAGTTAAAATGAAACTCCAGACCAACAACGCGTACCCAGCGTACGCCTTGCCGCTCTa                                                                                                                                   >  1:1611260/1‑150 (MQ=255)

                   agtCGGATTCAAAACGCGTGTTACCAAGGTTCTCGATGAATTAACGACTGATTATTTTTACTATTTTGGACATAAGGATTACATAAAGTCACAATTTAAACTGAACCTCCAGAAAACCAACGCTAACCCAGGGTACGCGTTGCCGCTCTa                                                                                                                                   <  2:1707784/148‑1 (MQ=255)

                      cGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACt                                                                                                                                <  1:1463306/150‑1 (MQ=255)

                      cGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCAAGACCGATGATTTTTACTATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACt                                                                                                                                >  2:989860/1‑150 (MQ=255)

                       ggCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACtt                                                                                                                               >  2:1697015/1‑150 (MQ=255)

                         cTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGTCATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACtttt                                                                                                                             >  1:845767/1‑150 (MQ=255)

                         cTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACtttt                                                                                                                             >  2:1704574/1‑150 (MQ=255)

                         cTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACtttt                                                                                                                             >  1:349912/1‑150 (MQ=255)

                         cTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAAAGCTTACCCAAGGTACGCGTAGACGCTCTAACtttt                                                                                                                             >  1:240405/1‑150 (MQ=255)

                           tCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATTATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACtttttt                                                                                                                           <  2:1512346/150‑1 (MQ=255)

                           tCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACtttttt                                                                                                                           <  1:1353755/150‑1 (MQ=255)

                            cAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCAGCTCTAACTTTTtta                                                                                                                          >  1:1560113/1‑150 (MQ=255)

                             aaCAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTttac                                                                                                                         <  2:608399/150‑1 (MQ=255)

                                   cGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAACCTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTacttactt                                                                                                                   >  2:937597/1‑150 (MQ=255)

                                   cGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTacttactt                                                                                                                   <  1:1088212/150‑1 (MQ=255)

                                       ttACCACGGGAGTCGATCATTTCACGACCGATGATTATTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGcc                                                                                                               <  2:866737/150‑1 (MQ=255)

                                            aCGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACg                                                                                                          <  2:1492351/150‑1 (MQ=255)

                                                gAGGCGATGATTTCACGACCTATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTc                                                                                                      <  2:291605/150‑1 (MQ=255)

                                                   tCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTgg                                                                                                   <  2:1574618/150‑1 (MQ=255)

                                                    cGATGATTTCACGACCGATGATTTTAACTATTTTGTACATTAGGTTTACCTCAAGTCACAAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGCTACGCGTTCCCGCTCTAACATATATAATTACTTCGCCTGACGTTTCTGGa                                                                                                  >  2:777593/1‑150 (MQ=255)

                                                     gatgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGaa                                                                                                 >  2:1326323/1‑150 (MQ=255)

                                                      atgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAAc                                                                                                <  1:1698317/150‑1 (MQ=255)

                                                       tgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACt                                                                                               <  2:1599079/150‑1 (MQ=255)

                                                       tgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACt                                                                                               <  2:1592575/150‑1 (MQ=255)

                                                        gatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTc                                                                                              >  2:1612726/1‑150 (MQ=255)

                                                         atTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCg                                                                                             >  2:987560/1‑150 (MQ=255)

                                                             cACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACACAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCATGACGTTTCTGGAACTCGCTgg                                                                                         >  1:124752/1‑150 (MQ=255)

                                                              aCGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTCCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggc                                                                                        <  2:1355293/150‑1 (MQ=255)

                                                              aCGACCGATGATTTTTACGATTTTGGACATTAGGGTTTCCTCAAGTCACTAGTTAAACTGAAACTCCTGACAACCAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggc                                                                                        <  2:92574/150‑1 (MQ=255)

                                                                     atgatTTTTACTATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCAAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTc                                                                                 <  2:374881/150‑1 (MQ=255)

                                                                     atgatTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTc                                                                                 <  1:1432560/150‑1 (MQ=255)

                                                                     atgatTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTc                                                                                 >  2:1438522/1‑150 (MQ=255)

                                                                         tttttACGATTTTTGACATTAGGTTTTCCTCAAGGCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACaa                                                                             <  2:1452338/150‑1 (MQ=255)

                                                                           tttACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGAGGTTTCTGGAACTCGCTGGCGGCTTTCACAAAg                                                                           >  1:447772/1‑150 (MQ=255)

                                                                                  ttttGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCaa                                                                    >  1:1440594/1‑150 (MQ=255)

                                                                                     tGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACa                                                                 >  2:1522267/1‑150 (MQ=255)

                                                                                       gACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGc                                                               <  1:1420307/150‑1 (MQ=255)

                                                                                        aCATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGCCAAACACCGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCg                                                              >  2:842681/1‑150 (MQ=255)

                                                                                        aCATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCg                                                              >  2:800816/1‑150 (MQ=255)

                                                                                         cATTAGGTTTTCCTCAAGTCACTAGTTAAACTGCAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCgg                                                             >  1:1013765/1‑150 (MQ=255)

                                                                                         cATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCgg                                                             >  2:838834/1‑150 (MQ=255)

                                                                                         cATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCgg                                                             >  2:959912/1‑150 (MQ=255)

                                                                                           ttAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGt                                                           <  1:1326323/150‑1 (MQ=255)

                                                                                             aGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGa                                                         >  2:357888/1‑150 (MQ=255)

                                                                                              ggTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAACCAGCGGGTGAc                                                        >  2:1067379/1‑150 (MQ=255)

                                                                                                   tCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCa                                                   >  1:510775/1‑150 (MQ=255)

                                                                                                   cccTCAAGCCACTATCTACACTGCACCACCCGCCAAACAACGCGTACCCCGGGCACGCGTTGCCGCTCTAACTTTTTTACTTCCTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCa                                                   <  1:289876/149‑1 (MQ=255)

                                                                                                    ccTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCAc                                                  >  1:1655774/1‑150 (MQ=255)

                                                                                                    ccTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCAc                                                  >  2:442467/1‑150 (MQ=255)

                                                                                                    ccTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCAc                                                  <  2:395276/150‑1 (MQ=255)

                                                                                                     cTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACg                                                 <  1:946748/150‑1 (MQ=255)

                                                                                                       cAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAACGCCTGCAAACAGCGGGTGACCATCACGTg                                               >  2:1111542/1‑150 (MQ=255)

                                                                                                       cAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTg                                               <  2:961802/150‑1 (MQ=255)

                                                                                                       cAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTg                                               <  1:1153531/150‑1 (MQ=255)

                                                                                                        aaGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGGCGCTCTAACTTTTTTACTTACGTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCCCAAAGCCTGCCAACAGAGGGTGCCCTTCCCGTgg                                              >  2:122668/1‑150 (MQ=255)

                                                                                                        aaGTAACTAGTTAAAGTTAAACTCCAGACAAACAACGCGTACCCCGGGTACGCGTTGCCGCTCTAACTTTTTTAGTTACTTCGGCTTACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTgg                                              <  2:807139/150‑1 (MQ=255)

                                                                                                          gTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGACGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAg                                            >  2:1107711/1‑150 (MQ=255)

                                                                                                           tCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGt                                           <  2:240405/150‑1 (MQ=255)

                                                                                                           tCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACCTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCAACACGTGGAGt                                           >  1:765037/1‑150 (MQ=255)

                                                                                                            cACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCa                                                                     >  2:609147/1‑123 (MQ=255)

                                                                                                            cACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCa                                                                     <  1:609147/123‑1 (MQ=255)

                                                                                                                aGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAg                                      >  2:1120978/1‑150 (MQ=255)

                                                                                                                 gTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGt                                     >  1:844601/1‑150 (MQ=255)

                                                                                                                 gTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGt                                     >  1:539175/1‑150 (MQ=255)

                                                                                                                   tAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCCCAAAGCCTGCAAACAGCGGGTGACCCTCACGTGGAGTAGAAGTaa                                   >  1:1321452/1‑150 (MQ=255)

                                                                                                                   tAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGc                                                                      <  2:297475/115‑1 (MQ=255)

                                                                                                                   tAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGc                                                                      >  1:297475/1‑115 (MQ=255)

                                                                                                                       cTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTc                               >  2:1292223/1‑150 (MQ=255)

                                                                                                                         gAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCg                             >  1:1697684/1‑150 (MQ=255)

                                                                                                                             cTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATg                         >  1:1286614/1‑150 (MQ=255)

                                                                                                                              tCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATgg                        <  1:442467/150‑1 (MQ=255)

                                                                                                                               ccAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGa                       >  2:1237375/1‑150 (MQ=255)

                                                                                                                                 aGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCGAACTATTTTACTTACTTCGCCTGACGTTTCTGGCACTCGATGGCGGCGTTCACAAAGCCTGCAAAAATCGGGTGAACAGCACGTGGAGTAGAAGTAAACTCCGGATGCAAc                     >  1:1275189/1‑150 (MQ=255)

                                                                                                                                   agcggcaacGCGTACCCTGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACGTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACCGCGGGAGAACCTCACGGGGAGGAGAAGTAACCTCCGGATGGAACTg                   >  1:345408/6‑150 (MQ=255)

                                                                                                                                   acaaacaaCGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTg                   >  2:1310935/1‑150 (MQ=255)

                                                                                                                                     aaacaaCGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGc                 <  1:410542/150‑1 (MQ=255)

                                                                                                                                      aacaacGCGTTCCCAGGGTACGCGTTGCCGCTCAAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACATAGACTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTGCGGATGGAACTGGCa                >  1:1095566/1‑150 (MQ=255)

                                                                                                                                      aacaacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCa                <  2:1376686/150‑1 (MQ=255)

                                                                                                                                       gcaacGCGTACCCTGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCATACAGCGGGTGACGATCAGGTGGAGTAGAAGTAAACTGCGGATGGCACTGGCac               >  1:103032/2‑149 (MQ=255)

                                                                                                                                         aacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGc             >  2:962655/1‑150 (MQ=255)

                                                                                                                                          acGCTTACCGCGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGTAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCTCGTGGAGTATAAGTAAACTCCGGATGGAACTGGCAAGcc            <  1:36058/150‑1 (MQ=255)

                                                                                                                                            gcgTACCCAGGGTACGCGTTGCAGATCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCAc          <  2:1047252/150‑1 (MQ=255)

                                                                                                                                                 cccAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAAcc     >  1:370221/1‑150 (MQ=255)

                                                                                                                                                    aGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGCCGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCTCGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACg  <  2:840428/150‑1 (MQ=255)

AAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACG  >  NC_000913/2907850‑2908147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`