Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,911,007 G→A 9.5% R29C (CGT→TGT)  mazF ← mRNA interferase toxin, antitoxin is MazE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,911,0070GA 9.5% 336.7 / 16.1 115R29C (CGT→TGT) mazFmRNA interferase toxin, antitoxin is MazE
Reads supporting (aligned to +/- strand):  ref base G (57/47);  new base A (4/7);  total (61/54)
Fisher's exact test for biased strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 117 total aligned reads displayed.

TACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGA  >  NC_000913/2910858‑2911155
                                                                                                                                                     |                                                                                                                                                     
tACCTGATGAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACg                                                                                                                                                       <  2:1235405/150‑1 (MQ=255)
tACCTGATCAGCTACCGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACg                                                                                                                                                       <  2:362681/150‑1 (MQ=255)
 aCCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGa                                                                                                                                                      >  2:915260/1‑150 (MQ=255)
  ccTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGAt                                                                                                                                                     >  2:716302/1‑150 (MQ=255)
      aTCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTcc                                                                                                                                                 >  1:785961/1‑150 (MQ=255)
      aTCAGCAAACGCTACGCCATCACGTACCTGACCGGATAAAACAACTTCGAACGGAAATCCTTTTGATTGCGTTCTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTcc                                                                                                                                                 <  2:342286/150‑1 (MQ=255)
         aGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGc                                                                                                                                              <  2:1166500/150‑1 (MQ=255)
          gCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCt                                                                                                                                             <  1:1040178/150‑1 (MQ=255)
           cTAACGCTACGCCATCACGTTCCTGACCGTATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACACGGACCACACAGACACATACCTGTGTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtg                                                                                                                                            <  1:1571197/150‑2 (MQ=255)
           cTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtg                                                                                                                                            <  2:554182/150‑2 (MQ=255)
            tAACGCTACGCCATCACGTTCCTGCCCGGAGAAAACAACTTCGAACGGATCTCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTg                                                                                                                                           >  1:1193542/1‑150 (MQ=255)
            tAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtgt                                                                                                                                           <  1:147957/150‑3 (MQ=255)
            tAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtgt                                                                                                                                           <  1:953046/150‑3 (MQ=255)
            tAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtgt                                                                                                                                           <  1:1502303/150‑3 (MQ=255)
               cGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTc                                                                                                                                        >  1:628777/1‑150 (MQ=255)
               cGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTc                                                                                                                                        <  1:959945/150‑1 (MQ=255)
                   aCGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTa                                                                                                                                    <  2:476681/150‑1 (MQ=255)
                        aTCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGAATCAGGACAACAGCTGGACGATGTCAAGATTGCTCGCTACCttt                                                                                                                               >  1:1559924/1‑150 (MQ=255)
                            cGTTCCTGACCTGATAAAACAACTTAGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTc                                                                                                                           <  1:1728733/150‑1 (MQ=255)
                            cGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTc                                                                                                                           <  2:1237511/150‑1 (MQ=255)
                               tCCTGTCCGGATAAAACAACTTCGAACGGTTATCCTTTTGATTGCGGTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAATGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGGCggg                                                                                                                        <  2:770331/150‑1 (MQ=255)
                               tCCTGACCGGATACAACAACTTCGAACGGATATCCTTTTGATAGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCggg                                                                                                                        <  1:308248/150‑1 (MQ=255)
                               tCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCggg                                                                                                                        <  2:1579646/150‑1 (MQ=255)
                               tCCTGACCGCATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCggg                                                                                                                        <  1:312246/150‑1 (MQ=255)
                                ccTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGt                                                                                                                       <  1:912299/150‑1 (MQ=255)
                                    aCCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCaaa                                                                                                                  >  1:671278/1‑150 (MQ=255)
                                       ggATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGCCACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATc                                                                                                               >  2:344719/1‑150 (MQ=255)
                                       ggATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATc                                                                                                               >  1:731375/1‑150 (MQ=255)
                                           aaaaCAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAcc                                                                                                           <  2:620729/150‑1 (MQ=255)
                                           aaaaCAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAcc                                                                                                           <  2:1448615/150‑1 (MQ=255)
                                            aaaCAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAccc                                                                                                          >  1:267495/1‑150 (MQ=255)
                                             aacaacTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCa                                                                                                         <  2:1150140/150‑1 (MQ=255)
                                               caacTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCaaa                                                                                                       <  2:662111/150‑1 (MQ=255)
                                                    tCGAACGGATATCCTTTTGATTGCGTTTTACAAGGAACACACAGACAAATACGTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGa                                                                                                  <  2:355114/150‑1 (MQ=255)
                                                             tatCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCata                                                                                         <  1:453765/150‑1 (MQ=255)
                                                             tatCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCata                                                                                         >  1:1306656/1‑150 (MQ=255)
                                                             tatCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTCGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGAACGCCCata                                                                                         >  1:1086895/1‑150 (MQ=255)
                                                                ccTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCg                                                                                      >  2:1502769/1‑150 (MQ=255)
                                                                       aTTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTc                                                                                                                                        >  2:910653/1‑94 (MQ=255)
                                                                       aTTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTc                                                                                                                                        <  1:910653/94‑1 (MQ=255)
                                                                           cGTTGTACAAGGAACACACAGACCCATACATGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGATCGCTACCTTTGGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCg                                                                           >  1:223776/1‑150 (MQ=255)
                                                                           cGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCg                                                                           >  1:1432173/1‑150 (MQ=255)
                                                                           cGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCg                                                                           >  2:320419/1‑150 (MQ=255)
                                                                             ttGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGGACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGc                                                                         <  1:1004301/150‑1 (MQ=255)
                                                                              tGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACACCAGCTGGCCGATGTCCCGCTTGCTAGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCATATCGCCCATATCGGGTAAGTTTCGGCt                                                                        >  1:286301/1‑150 (MQ=255)
                                                                                aaCAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAACGAATCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCTGCTCAAAATCAACCCAAATCAGATCCCCCCTATCGGGTACGTATCGGCtaa                                                                      >  2:1563999/2‑148 (MQ=255)
                                                                                 aCAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATc                                                                            >  2:295866/1‑143 (MQ=255)
                                                                                 aCAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttac                                                                     >  2:615001/1‑150 (MQ=255)
                                                                                   aaGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttacca                                                                   >  1:538661/1‑150 (MQ=255)
                                                                                    aGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccat                                                                  >  1:1427313/1‑150 (MQ=255)
                                                                                     ggAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccatt                                                                 >  1:715280/1‑150 (MQ=255)
                                                                                       aaCACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTCCCTTTTGTCGGGTCAACATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccattac                                                               >  2:523384/1‑150 (MQ=255)
                                                                                       aaCACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccattac                                                               >  2:826283/1‑150 (MQ=255)
                                                                                             caGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACt                                                         >  1:397598/1‑150 (MQ=255)
                                                                                               gACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACtt                                                        >  1:1511051/1‑150 (MQ=255)
                                                                                                acacATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGATCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTcc                                                      <  1:568500/150‑1 (MQ=255)
                                                                                                 cacaTACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCt                                                     <  1:1158299/150‑1 (MQ=255)
                                                                                                 cacaTACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCt                                                     >  2:1484817/1‑150 (MQ=255)
                                                                                                 cacaTACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACAAGACTTCCt                                                     >  2:1413326/1‑150 (MQ=255)
                                                                                                    aTACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTAt                                                  <  1:406824/150‑1 (MQ=255)
                                                                                                      aCCTGTGTTGTTGTTGTACATGATAGGACTCAGGACTTCAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATTTCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCt                                                <  2:1527121/150‑1 (MQ=255)
                                                                                                        cTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCttt                                              >  2:1117786/1‑150 (MQ=255)
                                                                                                              tgttgttgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGctc                                        >  2:1254366/1‑150 (MQ=255)
                                                                                                                ttgttgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCtctc                                      <  1:826283/150‑1 (MQ=255)
                                                                                                                 tgttgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGCCTTCCTTATCTTTCGGCTCTcc                                     >  2:1059507/1‑150 (MQ=255)
                                                                                                                    tgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCAAGCTTGCTCGCTACCTTTTGTCGGGTCAAATTCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCa                                  >  1:791173/1‑150 (MQ=255)
                                                                                                                     gtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGCCTTCCTTATCTTTCGGCTCTCCCCAg                                 >  1:1439392/1‑150 (MQ=255)
                                                                                                                     gtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAg                                 >  2:183611/1‑150 (MQ=255)
                                                                                                                     gtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAg                                 <  2:1432173/150‑1 (MQ=255)
                                                                                                                     gtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGGACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCTGATCTACCCAg                                 >  2:899768/1‑150 (MQ=255)
                                                                                                                     gtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATAAACCCATATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTGCTTATCTTTCGGCTCTCACCAg                                 >  2:650876/1‑150 (MQ=255)
                                                                                                                       aCATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTc                               <  2:1427313/150‑1 (MQ=255)
                                                                                                                       aCATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTc                               <  1:1329413/150‑1 (MQ=255)
                                                                                                                       aCATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTc                               <  2:679003/150‑1 (MQ=255)
                                                                                                                        cATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCCTATCTTTCGGCTCTCCCCAGTCg                              >  1:926580/1‑150 (MQ=255)
                                                                                                                          tGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTCCCAGCCTTCCTTATCTTTCGGCTCTCCCCAGTCGat                            >  1:1574748/1‑150 (MQ=255)
                                                                                                                           gAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAATTCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGata                           >  2:1436068/1‑150 (MQ=255)
                                                                                                                            aaaGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGatat                          <  1:1331296/150‑1 (MQ=255)
                                                                                                                            aaaGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGatat                          <  2:881055/150‑1 (MQ=255)
                                                                                                                                  cTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGt                    >  1:467001/1‑150 (MQ=255)
                                                                                                                                  cTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGt                    >  2:1192693/1‑150 (MQ=255)
                                                                                                                                      ggACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCACCCCAGTCGATATTCTCGTGGAg                <  2:731375/150‑1 (MQ=255)
                                                                                                                                       gACAACAGCTGGACGATTTACAGCTTGCTCGCTACCTTTTGTAGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGTTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAgg               <  2:1231273/150‑1 (MQ=255)
                                                                                                                                       gACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAgg               >  2:431/1‑150 (MQ=255)
                                                                                                                                       cgagcaaGCTGGACATCGTCCAGCTTGCTCGCTACCTTTTGTCGTGTCTAAATCAACCCAAATCAGATCGACCATATCGGGTACGTATCGGCTTACCATTTCCAGACTTAATAATCTTTCGGCTCTCCCCAGTAGATATTATAGTGGAgg               >  1:641443/7‑150 (MQ=255)
                                                                                                                                        acaacaGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGt              <  2:1183397/150‑1 (MQ=255)
                                                                                                                                         agcaaGCTGGACATCGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGtt             >  1:1290106/5‑150 (MQ=255)
                                                                                                                                         agcaaGCTGGACATCGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGtt             >  1:1460093/5‑150 (MQ=255)
                                                                                                                                          gcaaGCTGGACATCGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCCCCCATATCGGGTACGTATCGGCTGCCCATTACCAGAGTTGCTGAGCTTTCGGCGCTCACCAGTCGGTATTCGCGTGGAGGgtt            >  1:826646/4‑150 (MQ=255)
                                                                                                                                           acaGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGtttt           >  1:579621/1‑150 (MQ=255)
                                                                                                                                            caGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTc          <  1:915260/150‑1 (MQ=255)
                                                                                                                                             aGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTcc         <  1:1633804/150‑1 (MQ=255)
                                                                                                                                              gCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCg        >  2:141742/1‑150 (MQ=255)
                                                                                                                                              gCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCg        >  1:1008192/1‑150 (MQ=255)
                                                                                                                                               cTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCgg       >  1:1710802/1‑150 (MQ=255)
                                                                                                                                               cTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCgg       <  1:1247828/150‑1 (MQ=255)
                                                                                                                                                tGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGCCTTCCTTATCTTTCGGCTCTCCCCAGGCGATATTCTCGTGGAGGTTTTCCGGc      <  1:1059507/150‑1 (MQ=255)
                                                                                                                                                 ggACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCg     <  1:1577195/150‑1 (MQ=255)
                                                                                                                                                  gACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGt    >  1:199389/1‑150 (MQ=255)
                                                                                                                                                    cGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCACAGACGATTTTCGCGTGGAGGTTTTCCGGCGTGa  >  2:1324649/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGA  >  NC_000913/2910858‑2911155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: