Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,911,008 A→T 9.6% H28Q (CAT→CAA) ‡ mazF ← mRNA interferase toxin, antitoxin is MazE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,911,0080AT 9.6% 297.9 / 16.8 114H28Q (CAT→CAA) ‡mazFmRNA interferase toxin, antitoxin is MazE
Reads supporting (aligned to +/- strand):  ref base A (56/47);  new base T (4/7);  total (60/54)
Fisher's exact test for biased strand distribution p-value = 3.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 115 total aligned reads displayed.

ACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGA  >  NC_000913/2910859‑2911155
                                                                                                                                                     |                                                                                                                                                    
aCCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGa                                                                                                                                                      >  2:915260/1‑150 (MQ=255)
 ccTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGAt                                                                                                                                                     >  2:716302/1‑150 (MQ=255)
   tGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGt                                                                                                                                                   >  1:881055/1‑150 (MQ=255)
     aTCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTcc                                                                                                                                                 >  1:785961/1‑150 (MQ=255)
        aGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGc                                                                                                                                              <  2:1166500/150‑1 (MQ=255)
         gCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCt                                                                                                                                             <  1:1040178/150‑1 (MQ=255)
          cTAACGCTACGCCATCACGTTCCTGACCGTATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACACGGACCACACAGACACATACCTGTGTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtg                                                                                                                                            <  1:1571197/150‑2 (MQ=255)
          cTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtg                                                                                                                                            <  2:554182/150‑2 (MQ=255)
           tAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtgt                                                                                                                                           <  1:953046/150‑3 (MQ=255)
           tAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtgt                                                                                                                                           <  1:147957/150‑3 (MQ=255)
           tAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtgt                                                                                                                                           <  1:1502303/150‑3 (MQ=255)
              cGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTc                                                                                                                                        <  1:959945/150‑1 (MQ=255)
                  aCGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTa                                                                                                                                    <  2:476681/150‑1 (MQ=255)
                           cGTTCCTGACCTGATAAAACAACTTAGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTc                                                                                                                           <  1:1728733/150‑1 (MQ=255)
                           cGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTc                                                                                                                           <  2:1237511/150‑1 (MQ=255)
                              tCCTGACCGGATACAACAACTTCGAACGGATATCCTTTTGATAGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCggg                                                                                                                        <  1:308248/150‑1 (MQ=255)
                              tCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCggg                                                                                                                        <  2:1579646/150‑1 (MQ=255)
                              tCCTGACCGCATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCggg                                                                                                                        <  1:312246/150‑1 (MQ=255)
                               ccTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGt                                                                                                                       <  1:912299/150‑1 (MQ=255)
                                   aCCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCaaa                                                                                                                  >  1:671278/1‑150 (MQ=255)
                                     cGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAAt                                                                                                                <  1:1679389/150‑1 (MQ=255)
                                      ggATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGCCACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATc                                                                                                               >  2:344719/1‑150 (MQ=255)
                                      ggATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATc                                                                                                               >  1:731375/1‑150 (MQ=255)
                                          aaaaCAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAcc                                                                                                           <  2:620729/150‑1 (MQ=255)
                                          aaaaCAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAcc                                                                                                           <  2:1448615/150‑1 (MQ=255)
                                           aaaCAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAccc                                                                                                          >  1:267495/1‑150 (MQ=255)
                                            aacaacTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCa                                                                                                         <  2:1150140/150‑1 (MQ=255)
                                              caacTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCaaa                                                                                                       <  2:662111/150‑1 (MQ=255)
                                                   tCGAACGGATATCCTTTTGATTGCGTTTTACAAGGAACACACAGACAAATACGTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGa                                                                                                  <  2:355114/150‑1 (MQ=255)
                                                            tatCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCata                                                                                         >  1:1306656/1‑150 (MQ=255)
                                                            tatCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCata                                                                                         <  1:453765/150‑1 (MQ=255)
                                                            tatCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTCGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGAACGCCCata                                                                                         >  1:1086895/1‑150 (MQ=255)
                                                               ccTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCg                                                                                      >  2:1502769/1‑150 (MQ=255)
                                                               ccTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCg                                                                                      <  2:785961/150‑1 (MQ=255)
                                                                   ttGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTa                                                                                  >  2:1331296/1‑150 (MQ=255)
                                                                   ttGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTa                                                                                  <  2:393727/150‑1 (MQ=255)
                                                                      aTTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTc                                                                                                                                        <  1:910653/94‑1 (MQ=255)
                                                                      aTTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTc                                                                                                                                        >  2:910653/1‑94 (MQ=255)
                                                                          cGTTGTACAAGGAACACACAGACCCATACATGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGATCGCTACCTTTGGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCg                                                                           >  1:223776/1‑150 (MQ=255)
                                                                          cGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCg                                                                           >  2:320419/1‑150 (MQ=255)
                                                                            ttGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGGACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGc                                                                         <  1:1004301/150‑1 (MQ=255)
                                                                             tGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACACCAGCTGGCCGATGTCCCGCTTGCTAGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCATATCGCCCATATCGGGTAAGTTTCGGCt                                                                        >  1:286301/1‑150 (MQ=255)
                                                                               aaCAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAACGAATCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCTGCTCAAAATCAACCCAAATCAGATCCCCCCTATCGGGTACGTATCGGCtaa                                                                      >  2:1563999/2‑148 (MQ=255)
                                                                                aCAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATc                                                                            >  2:295866/1‑143 (MQ=255)
                                                                                aCAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATc                                                                            <  1:295866/143‑1 (MQ=255)
                                                                                 cAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttacc                                                                    <  2:1478730/150‑1 (MQ=255)
                                                                                  aaGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttacca                                                                   >  1:538661/1‑150 (MQ=255)
                                                                                   aGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccat                                                                  >  1:679003/1‑150 (MQ=255)
                                                                                   aGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccat                                                                  >  1:1427313/1‑150 (MQ=255)
                                                                                    ggAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccatt                                                                 >  1:715280/1‑150 (MQ=255)
                                                                                      aaCACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTCCCTTTTGTCGGGTCAACATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccattac                                                               >  2:523384/1‑150 (MQ=255)
                                                                                      aaCACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccattac                                                               >  2:826283/1‑150 (MQ=255)
                                                                                           acaGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGAc                                                          <  1:52418/150‑1 (MQ=255)
                                                                                            caGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACt                                                         >  1:397598/1‑150 (MQ=255)
                                                                                              gACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACtt                                                        >  1:1511051/1‑150 (MQ=255)
                                                                                               acacATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGATCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTcc                                                      <  1:568500/150‑1 (MQ=255)
                                                                                                cacaTACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCt                                                     <  1:1158299/150‑1 (MQ=255)
                                                                                                cacaTACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCt                                                     >  2:1484817/1‑150 (MQ=255)
                                                                                                cacaTACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACAAGACTTCCt                                                     >  2:1413326/1‑150 (MQ=255)
                                                                                                   aTACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTAt                                                  <  2:628777/150‑1 (MQ=255)
                                                                                                     aCCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCt                                                >  1:1423342/1‑150 (MQ=255)
                                                                                                       cTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCttt                                              >  2:1117786/1‑150 (MQ=255)
                                                                                                             tgttgttgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGctc                                        >  2:1254366/1‑150 (MQ=255)
                                                                                                                tgttgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGCCTTCCTTATCTTTCGGCTCTcc                                     >  2:1059507/1‑150 (MQ=255)
                                                                                                                tgttgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCGCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTcc                                     <  2:267495/150‑1 (MQ=255)
                                                                                                                   tgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCa                                  >  1:1173953/1‑150 (MQ=255)
                                                                                                                   tgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCAAGCTTGCTCGCTACCTTTTGTCGGGTCAAATTCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCa                                  >  1:791173/1‑150 (MQ=255)
                                                                                                                    gtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGCCTTCCTTATCTTTCGGCTCTCCCCAg                                 >  1:1439392/1‑150 (MQ=255)
                                                                                                                    gtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAg                                 <  2:1432173/150‑1 (MQ=255)
                                                                                                                    gtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAg                                 >  2:183611/1‑150 (MQ=255)
                                                                                                                    gtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGGACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCTGATCTACCCAg                                 >  2:899768/1‑150 (MQ=255)
                                                                                                                    gtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATAAACCCATATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTGCTTATCTTTCGGCTCTCACCAg                                 >  2:650876/1‑150 (MQ=255)
                                                                                                                      aCATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTc                               <  2:679003/150‑1 (MQ=255)
                                                                                                                      aCATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTc                               <  2:1427313/150‑1 (MQ=255)
                                                                                                                      aCATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTc                               <  1:1329413/150‑1 (MQ=255)
                                                                                                                       cATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCCTATCTTTCGGCTCTCCCCAGTCg                              >  1:926580/1‑150 (MQ=255)
                                                                                                                         tGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTCCCAGCCTTCCTTATCTTTCGGCTCTCCCCAGTCGat                            >  1:1574748/1‑150 (MQ=255)
                                                                                                                          gAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAATTCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGata                           >  2:1436068/1‑150 (MQ=255)
                                                                                                                           aaaGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGatat                          <  1:1331296/150‑1 (MQ=255)
                                                                                                                           aaaGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGatat                          <  2:881055/150‑1 (MQ=255)
                                                                                                                                 cTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGt                    >  2:1192693/1‑150 (MQ=255)
                                                                                                                                 cTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGt                    >  1:467001/1‑150 (MQ=255)
                                                                                                                                     ggACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCACCCCAGTCGATATTCTCGTGGAg                <  2:731375/150‑1 (MQ=255)
                                                                                                                                      gACAACAGCTGGACGATTTACAGCTTGCTCGCTACCTTTTGTAGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGTTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAgg               <  2:1231273/150‑1 (MQ=255)
                                                                                                                                      gACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAgg               >  2:431/1‑150 (MQ=255)
                                                                                                                                      cgagcaaGCTGGACATCGTCCAGCTTGCTCGCTACCTTTTGTCGTGTCTAAATCAACCCAAATCAGATCGACCATATCGGGTACGTATCGGCTTACCATTTCCAGACTTAATAATCTTTCGGCTCTCCCCAGTAGATATTATAGTGGAgg               >  1:641443/7‑150 (MQ=255)
                                                                                                                                       acaacaGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGt              <  1:1502769/150‑1 (MQ=255)
                                                                                                                                       acaacaGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGt              <  2:1183397/150‑1 (MQ=255)
                                                                                                                                        agcaaGCTGGACATCGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGtt             >  1:1290106/5‑150 (MQ=255)
                                                                                                                                        agcaaGCTGGACATCGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGtt             >  1:1460093/5‑150 (MQ=255)
                                                                                                                                         gcaaGCTGGACATCGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCCCCCATATCGGGTACGTATCGGCTGCCCATTACCAGAGTTGCTGAGCTTTCGGCGCTCACCAGTCGGTATTCGCGTGGAGGgtt            >  1:826646/4‑150 (MQ=255)
                                                                                                                                          acaGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGtttt           >  1:579621/1‑150 (MQ=255)
                                                                                                                                           caGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTc          <  1:915260/150‑1 (MQ=255)
                                                                                                                                            aGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTcc         <  1:1633804/150‑1 (MQ=255)
                                                                                                                                             gCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCg        >  1:1008192/1‑150 (MQ=255)
                                                                                                                                              cTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCgg       <  1:1247828/150‑1 (MQ=255)
                                                                                                                                              cTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCgg       >  1:1710802/1‑150 (MQ=255)
                                                                                                                                               tGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGCCTTCCTTATCTTTCGGCTCTCCCCAGGCGATATTCTCGTGGAGGTTTTCCGGc      <  1:1059507/150‑1 (MQ=255)
                                                                                                                                                ggACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCg     <  1:1577195/150‑1 (MQ=255)
                                                                                                                                                   cGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCACAGACGATTTTCGCGTGGAGGTTTTCCGGCGTGa  >  2:1324649/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGA  >  NC_000913/2910859‑2911155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: