Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,911,009 T→C 9.7% H28R (CAT→CGT) ‡ mazF ← mRNA interferase toxin, antitoxin is MazE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,911,0090TC 9.7% 304.0 / 19.9 113H28R (CAT→CGT) ‡mazFmRNA interferase toxin, antitoxin is MazE
Reads supporting (aligned to +/- strand):  ref base T (55/47);  new base C (4/7);  total (59/54)
Fisher's exact test for biased strand distribution p-value = 3.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

Only 100 of 115 total aligned reads displayed.

CCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGT  >  NC_000913/2910860‑2911158
                                                                                                                                                     |                                                                                                                                                     
ccTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGAt                                                                                                                                                       >  2:716302/1‑150 (MQ=255)
  tGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGt                                                                                                                                                     >  1:881055/1‑150 (MQ=255)
    aTCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTcc                                                                                                                                                   >  1:785961/1‑150 (MQ=255)
    aTCAGCAAACGCTACGCCATCACGTACCTGACCGGATAAAACAACTTCGAACGGAAATCCTTTTGATTGCGTTCTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTcc                                                                                                                                                   <  2:342286/150‑1 (MQ=255)
       aGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGc                                                                                                                                                <  2:1166500/150‑1 (MQ=255)
        gCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCt                                                                                                                                               <  1:1040178/150‑1 (MQ=255)
         cTAACGCTACGCCATCACGTTCCTGACCGTATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACACGGACCACACAGACACATACCTGTGTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtg                                                                                                                                              <  1:1571197/150‑2 (MQ=255)
          tAACGCTACGCCATCACGTTCCTGCCCGGAGAAAACAACTTCGAACGGATCTCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTg                                                                                                                                             >  1:1193542/1‑150 (MQ=255)
          tAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtgt                                                                                                                                             <  1:1502303/150‑3 (MQ=255)
          tAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtgt                                                                                                                                             <  1:147957/150‑3 (MQ=255)
             cGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTc                                                                                                                                          >  1:628777/1‑150 (MQ=255)
             cGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTc                                                                                                                                          <  1:959945/150‑1 (MQ=255)
                 aCGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTa                                                                                                                                      <  2:476681/150‑1 (MQ=255)
                      aTCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGAATCAGGACAACAGCTGGACGATGTCAAGATTGCTCGCTACCttt                                                                                                                                 >  1:1559924/1‑150 (MQ=255)
                          cGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTc                                                                                                                             <  2:1237511/150‑1 (MQ=255)
                             tCCTGTCCGGATAAAACAACTTCGAACGGTTATCCTTTTGATTGCGGTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAATGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGGCggg                                                                                                                          <  2:770331/150‑1 (MQ=255)
                             tCCTGACCGGATACAACAACTTCGAACGGATATCCTTTTGATAGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCggg                                                                                                                          <  1:308248/150‑1 (MQ=255)
                             tCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCggg                                                                                                                          <  2:1579646/150‑1 (MQ=255)
                             tCCTGACCGCATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCggg                                                                                                                          <  1:312246/150‑1 (MQ=255)
                              ccTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGt                                                                                                                         <  1:912299/150‑1 (MQ=255)
                                  aCCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCaaa                                                                                                                     >  1:671278/1‑150 (MQ=255)
                                    cGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAAt                                                                                                                   <  1:1679389/150‑1 (MQ=255)
                                     ggATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGCCACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATc                                                                                                                  >  2:344719/1‑150 (MQ=255)
                                     ggATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATc                                                                                                                  >  1:731375/1‑150 (MQ=255)
                                       aTAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCaa                                                                                                                >  1:956396/1‑150 (MQ=255)
                                         aaaaCAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAcc                                                                                                              <  2:1448615/150‑1 (MQ=255)
                                         aaaaCAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAcc                                                                                                              <  2:620729/150‑1 (MQ=255)
                                          aaaCAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAccc                                                                                                             >  1:267495/1‑150 (MQ=255)
                                           aacaacTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCa                                                                                                            <  2:1150140/150‑1 (MQ=255)
                                             caacTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCaaa                                                                                                          <  2:662111/150‑1 (MQ=255)
                                                  tCGAACGGATATCCTTTTGATTGCGTTTTACAAGGAACACACAGACAAATACGTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGa                                                                                                     <  2:355114/150‑1 (MQ=255)
                                                           tatCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCata                                                                                            >  1:1306656/1‑150 (MQ=255)
                                                           tatCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCata                                                                                            <  1:453765/150‑1 (MQ=255)
                                                           tatCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTCGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGAACGCCCata                                                                                            >  1:1086895/1‑150 (MQ=255)
                                                              ccTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCg                                                                                         <  2:785961/150‑1 (MQ=255)
                                                                  ttGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTa                                                                                     >  2:1331296/1‑150 (MQ=255)
                                                                  ttGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTa                                                                                     <  2:393727/150‑1 (MQ=255)
                                                                     aTTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTc                                                                                                                                          <  1:910653/94‑1 (MQ=255)
                                                                     aTTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTc                                                                                                                                          >  2:910653/1‑94 (MQ=255)
                                                                         cGTTGTACAAGGAACACACAGACCCATACATGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGATCGCTACCTTTGGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCg                                                                              >  1:223776/1‑150 (MQ=255)
                                                                         cGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCg                                                                              >  1:1432173/1‑150 (MQ=255)
                                                                           ttGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGGACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGc                                                                            <  1:1004301/150‑1 (MQ=255)
                                                                            tGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACACCAGCTGGCCGATGTCCCGCTTGCTAGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCATATCGCCCATATCGGGTAAGTTTCGGCt                                                                           >  1:286301/1‑150 (MQ=255)
                                                                              aaCAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAACGAATCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCTGCTCAAAATCAACCCAAATCAGATCCCCCCTATCGGGTACGTATCGGCtaa                                                                         >  2:1563999/2‑148 (MQ=255)
                                                                               aCAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATc                                                                               >  2:295866/1‑143 (MQ=255)
                                                                               aCAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATc                                                                               <  1:295866/143‑1 (MQ=255)
                                                                               aCAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttac                                                                        >  2:615001/1‑150 (MQ=255)
                                                                                cAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttacc                                                                       <  2:1478730/150‑1 (MQ=255)
                                                                                 aaGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttacca                                                                      >  1:538661/1‑150 (MQ=255)
                                                                                  aGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccat                                                                     >  1:679003/1‑150 (MQ=255)
                                                                                   ggAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccatt                                                                    >  1:715280/1‑150 (MQ=255)
                                                                                     aaCACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTCCCTTTTGTCGGGTCAACATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccattac                                                                  >  2:523384/1‑150 (MQ=255)
                                                                                     aaCACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccattac                                                                  >  2:826283/1‑150 (MQ=255)
                                                                                          acaGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGAc                                                             <  1:52418/150‑1 (MQ=255)
                                                                                              acacATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGATCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTcc                                                         <  1:568500/150‑1 (MQ=255)
                                                                                               cacaTACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCt                                                        <  1:1158299/150‑1 (MQ=255)
                                                                                               cacaTACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCt                                                        >  2:1484817/1‑150 (MQ=255)
                                                                                                  aTACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTAt                                                     <  2:628777/150‑1 (MQ=255)
                                                                                                  aTACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTAt                                                     <  1:406824/150‑1 (MQ=255)
                                                                                                    aCCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCt                                                   >  1:1423342/1‑150 (MQ=255)
                                                                                                    aCCTGTGTTGTTGTTGTACATGATAGGACTCAGGACTTCAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATTTCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCt                                                   <  2:1527121/150‑1 (MQ=255)
                                                                                                      cTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCttt                                                 >  2:1117786/1‑150 (MQ=255)
                                                                                                            tgttgttgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGctc                                           >  2:1254366/1‑150 (MQ=255)
                                                                                                              ttgttgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCtctc                                         <  1:826283/150‑1 (MQ=255)
                                                                                                               tgttgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGCCTTCCTTATCTTTCGGCTCTcc                                        >  2:1059507/1‑150 (MQ=255)
                                                                                                               tgttgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCGCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTcc                                        <  2:267495/150‑1 (MQ=255)
                                                                                                                   gtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGCCTTCCTTATCTTTCGGCTCTCCCCAg                                    >  1:1439392/1‑150 (MQ=255)
                                                                                                                   gtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAg                                    >  2:183611/1‑150 (MQ=255)
                                                                                                                   gtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAg                                    <  2:1432173/150‑1 (MQ=255)
                                                                                                                   gtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGGACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCTGATCTACCCAg                                    >  2:899768/1‑150 (MQ=255)
                                                                                                                   gtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATAAACCCATATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTGCTTATCTTTCGGCTCTCACCAg                                    >  2:650876/1‑150 (MQ=255)
                                                                                                                     aCATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTc                                  <  2:679003/150‑1 (MQ=255)
                                                                                                                     aCATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTc                                  <  1:1329413/150‑1 (MQ=255)
                                                                                                                     aCATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTc                                  <  2:1427313/150‑1 (MQ=255)
                                                                                                                      cATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCCTATCTTTCGGCTCTCCCCAGTCg                                 >  1:926580/1‑150 (MQ=255)
                                                                                                                        tGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTCCCAGCCTTCCTTATCTTTCGGCTCTCCCCAGTCGat                               >  1:1574748/1‑150 (MQ=255)
                                                                                                                         gAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAATTCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGata                              >  2:1436068/1‑150 (MQ=255)
                                                                                                                          aaaGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGatat                             <  2:881055/150‑1 (MQ=255)
                                                                                                                          aaaGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGatat                             <  1:1331296/150‑1 (MQ=255)
                                                                                                                                cTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCgg                       >  2:1185839/1‑149 (MQ=255)
                                                                                                                                cTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGt                       >  1:467001/1‑150 (MQ=255)
                                                                                                                                cTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGt                       >  2:1192693/1‑150 (MQ=255)
                                                                                                                                     gACAACAGCTGGACGATTTACAGCTTGCTCGCTACCTTTTGTAGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGTTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAgg                  <  2:1231273/150‑1 (MQ=255)
                                                                                                                                     gACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAgg                  >  2:431/1‑150 (MQ=255)
                                                                                                                                     cgagcaaGCTGGACATCGTCCAGCTTGCTCGCTACCTTTTGTCGTGTCTAAATCAACCCAAATCAGATCGACCATATCGGGTACGTATCGGCTTACCATTTCCAGACTTAATAATCTTTCGGCTCTCCCCAGTAGATATTATAGTGGAgg                  >  1:641443/7‑150 (MQ=255)
                                                                                                                                      acaacaGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGt                 <  1:1502769/150‑1 (MQ=255)
                                                                                                                                       agcaaGCTGGACATCGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGtt                >  1:1290106/5‑150 (MQ=255)
                                                                                                                                       agcaaGCTGGACATCGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGtt                >  1:1460093/5‑150 (MQ=255)
                                                                                                                                        gcaaGCTGGACATCGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCCCCCATATCGGGTACGTATCGGCTGCCCATTACCAGAGTTGCTGAGCTTTCGGCGCTCACCAGTCGGTATTCGCGTGGAGGgtt               >  1:826646/4‑150 (MQ=255)
                                                                                                                                          caGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTc             <  1:915260/150‑1 (MQ=255)
                                                                                                                                           aGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTcc            <  1:1633804/150‑1 (MQ=255)
                                                                                                                                            gCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCg           >  2:141742/1‑150 (MQ=255)
                                                                                                                                            gCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCg           >  1:1008192/1‑150 (MQ=255)
                                                                                                                                             cTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCgg          >  1:1710802/1‑150 (MQ=255)
                                                                                                                                             cTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCgg          <  1:1247828/150‑1 (MQ=255)
                                                                                                                                              tGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGCCTTCCTTATCTTTCGGCTCTCCCCAGGCGATATTCTCGTGGAGGTTTTCCGGc         <  1:1059507/150‑1 (MQ=255)
                                                                                                                                               ggACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCg        <  1:1577195/150‑1 (MQ=255)
                                                                                                                                                gACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGt       >  1:199389/1‑150 (MQ=255)
                                                                                                                                                  cGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCACAGACGATTTTCGCGTGGAGGTTTTCCGGCGTGa     >  2:1324649/1‑150 (MQ=255)
                                                                                                                                                     tGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTATCGTGGAGGTTTTCCGGCTTGATGt  >  1:1142569/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGT  >  NC_000913/2910860‑2911158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: