Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,918,019 G→T 10.3% intergenic (+206/+26) barA → / ← gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,918,0190GT10.3% 378.4 / 23.7 141intergenic (+206/+26)barA/gudDhybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1
Reads supporting (aligned to +/- strand):  ref base G (72/54);  new base T (5/10);  total (77/64)
Fisher's exact test for biased strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

Only 100 of 142 total aligned reads displayed.

CAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATC  >  NC_000913/2917870‑2918165
                                                                                                                                                     |                                                                                                                                                  
cAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACACCAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAg                                                                                                                                                    >  2:689925/1‑150 (MQ=255)
   aGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATa                                                                                                                                                 <  2:1064262/150‑1 (MQ=255)
     tGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATaga                                                                                                                                               >  1:1419112/1‑150 (MQ=255)
        cTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGCGcc                                                                                                                                            >  2:875456/1‑150 (MQ=255)
         tgtgTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCg                                                                                                                                           >  1:531162/1‑150 (MQ=255)
           tttgtaggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCAAGATAGAGCCggt                                                                                                                                         >  2:1688563/8‑150 (MQ=38)
            ttttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggtt                                                                                                                                        >  1:303291/2‑150 (MQ=255)
             tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttt                                                                                                                                       <  1:716812/150‑1 (MQ=255)
              ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttg                                                                                                                                      <  1:664293/150‑2 (MQ=255)
              ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAAATAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttg                                                                                                                                      <  2:1197355/150‑2 (MQ=38)
                aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttgg                                                                                                                                    <  1:1665144/150‑1 (MQ=255)
                aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttgg                                                                                                                                    <  1:1477958/150‑1 (MQ=255)
                 tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttgaga                                                                                                                                   <  1:30055/150‑5 (MQ=38)
                 ggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGAATCAGTCTCAAACCGGCTCTATCTGGAGCCggttttggt                                                                                                                                   >  1:1549449/2‑150 (MQ=255)
                   ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggttt                                                                                                                                 <  2:1493684/150‑1 (MQ=255)
                    cggttaaggcgTTAACGCCGCATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGCCCTGATACATAGCACAACAGGCTATTTTGCGGTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggtttt                                                                                                                                >  1:1244643/8‑150 (MQ=255)
                    cGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggtttt                                                                                                                                >  1:984527/1‑150 (MQ=255)
                    cGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCACTCTCAAACCGGCTCCAGATAGAGCCggttttggtttt                                                                                                                                >  2:1597961/1‑150 (MQ=255)
                     ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTc                                                                                                                               <  1:1025606/150‑1 (MQ=255)
                     ggATGCGGCGTTAACGCCTTACCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTc                                                                                                                               <  1:358495/150‑1 (MQ=255)
                            gcgTTAACGCCTTATCCGGCCTACAAAACCAATTTAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACATGCTATTTTGCGTTTATCATCAGTCTCAAACCGGCACGATAAAGAGCCGGTTTTGGTTTTGTGTCttt                                                                                                                        >  2:1083403/1‑149 (MQ=38)
                             cgTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGGCCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGAATGGTTTTCTGTCTTAAc                                                                                                                      >  1:1597749/1‑150 (MQ=255)
                                 aaCGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCa                                                                                                                   >  2:659106/1‑150 (MQ=255)
                                 aaCGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCa                                                                                                                   >  2:42384/1‑150 (MQ=255)
                                 aaCGCCTTATCCGGCCTACAAAACAAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACACCAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGAGAGAGCCGGTTTTGGTTTTCTGTCTTAACgaa                                                                                                                   >  2:273373/1‑148 (MQ=255)
                                   cGCCTTATCCGGCCTACAAAACCTATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCAcc                                                                                                                 >  2:176949/1‑150 (MQ=255)
                                    gcattATCCGGCCTACAAAACCAATTAAATTGAATGAATTAAAAATTGATGTAGAACTGATACATAGCACAACAGGCTATTTTGTGTTTAGCATCAGTCTCAAACCGGCTCCCGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCa                                                                                                                <  2:855005/147‑1 (MQ=255)
                                      cTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTTCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATg                                                                                                              <  1:785396/150‑1 (MQ=255)
                                        tATGCGGCCTACAATACCAATTAAATTCAATGAATTAAAAAATGTTGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCa                                                                                                            <  2:190792/150‑1 (MQ=255)
                                        tATCAGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCa                                                                                                            <  1:248965/150‑1 (MQ=255)
                                          tCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACg                                                                                                          >  1:1028526/1‑150 (MQ=255)
                                                cTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGAGCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCGGGTTTTGGTTTTCTGTCTTAACGCACCCTGCAcgcgcgat                                                                                                    >  2:93597/1‑148 (MQ=255)
                                                cTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCt                                                                                                    <  1:1388382/150‑1 (MQ=255)
                                                    aaaaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGtt                                                                                                >  1:1292957/1‑150 (MQ=255)
                                                    aaaaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGtt                                                                                                >  1:1344895/1‑150 (MQ=255)
                                                      aaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTAt                                                                                              <  1:1458023/150‑1 (MQ=255)
                                                       aCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCGATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATc                                                                                             <  1:207087/150‑1 (MQ=255)
                                                        caaaTTAAATTCAATTAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCg                                                                                            <  2:666264/148‑1 (MQ=255)
                                                         cAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTGTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGa                                                                                           >  2:242292/1‑150 (MQ=255)
                                                            ttAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACg                                                                                        <  2:234583/150‑1 (MQ=255)
                                                            ttAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACg                                                                                        <  1:1694182/150‑1 (MQ=255)
                                                            ttAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACg                                                                                        <  2:766184/150‑1 (MQ=255)
                                                               aattaaattaATTAAATAATGATGTAGTCCTGATACATAGCAGTACATGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTcc                                                                                     <  2:1567863/150‑1 (MQ=255)
                                                               aaTTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACTTcc                                                                                     <  2:873024/150‑1 (MQ=255)
                                                                aTTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTcca                                                                                    >  2:1330019/1‑150 (MQ=255)
                                                                   cAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTccagcc                                                                                 <  1:1411718/150‑1 (MQ=255)
                                                                     aTGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGGTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTccagccag                                                                               <  2:1594899/150‑1 (MQ=255)
                                                                       gAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGCGCCGGTTTTGTTTTTCTGTATTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCAAGGa                                                                             >  1:1280628/1‑150 (MQ=255)
                                                                       gAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGa                                                                             >  2:626845/1‑150 (MQ=255)
                                                                        aaTTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGat                                                                            >  1:1217169/1‑149 (MQ=255)
                                                                        aaTTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGaa                                                                            <  2:862090/150‑1 (MQ=255)
                                                                        aaTTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGaa                                                                            <  1:395569/150‑1 (MQ=255)
                                                                          ttAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAAta                                                                          >  1:154177/1‑149 (MQ=255)
                                                                           tAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCa                                                                         <  1:292117/150‑1 (MQ=255)
                                                                               aaaTGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATa                                                                     >  2:409872/1‑150 (MQ=255)
                                                                               aaaTGATGTAGACCTGATACATAGCACAACACTCTATTTTGCGTTTAGCATCCGTCTCACACCGGCTCCAGATAGCGCCGGGTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATa                                                                     <  1:853588/150‑1 (MQ=255)
                                                                                 atgatgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACt                                                                   >  1:595846/1‑150 (MQ=255)
                                                                                    atgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCa                                                                >  1:1448332/1‑150 (MQ=255)
                                                                                       tAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGAACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTc                                                             >  1:851934/1‑150 (MQ=255)
                                                                                        aGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATTAGATACTGCATTcc                                                            <  2:303291/150‑1 (MQ=255)
                                                                                           ccagATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACTCACCATCCACGGTCGCTTGTTATCGAACCTCCAGCCAGGAATCAGAAACTGCATTCCcat                                                         >  2:1327343/4‑150 (MQ=255)
                                                                                                tACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGCGCCGGTTTTGGTTTTCTGTCTTAACGCCCCATGCCCGGGCGCTTGTTATAGAACGTCCAGCCAGGAATCATATACTGCATTCCcatcgcat                                                    >  2:1456942/1‑150 (MQ=255)
                                                                                                tACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGCTACTGCATTCCcatcgccg                                                    >  2:1635810/1‑148 (MQ=255)
                                                                                                aaCATAGCACAACAGGCTATTTTGTGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCcatcgcat                                                    <  1:1178943/149‑1 (MQ=255)
                                                                                                  cATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCcatcgcatcg                                                  <  1:1086435/150‑1 (MQ=255)
                                                                                                  cATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCcatcgcatcg                                                  >  1:1114592/1‑150 (MQ=255)
                                                                                                  cATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGGTTTGGTTTTCTGTCTTAACGCACCATTCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCCTTCCcatcgcatag                                                  >  2:734019/1‑148 (MQ=255)
                                                                                                    tAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTc                                                <  1:659106/150‑1 (MQ=255)
                                                                                                     aGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCa                                               <  1:171327/150‑1 (MQ=255)
                                                                                                     aGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCa                                               <  1:176949/150‑1 (MQ=255)
                                                                                                      gCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCAc                                              <  2:572869/150‑1 (MQ=255)
                                                                                                            caGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACgcgcgc                                        <  2:1101430/150‑1 (MQ=255)
                                                                                                              ggCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCa                                      >  1:995621/1‑150 (MQ=255)
                                                                                                                    tttGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCg                                <  1:1324687/150‑1 (MQ=255)
                                                                                                                          tttAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGGTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTc                          >  1:770631/1‑150 (MQ=255)
                                                                                                                           ttAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCt                         >  2:1533950/1‑150 (MQ=255)
                                                                                                                             aGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGa                       <  1:1381394/150‑1 (MQ=255)
                                                                                                                              gCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGAt                      <  2:1712823/150‑1 (MQ=255)
                                                                                                                              gCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGAt                      <  1:1136712/150‑1 (MQ=255)
                                                                                                                              gCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATAGTCACGCGCGCCAAGCCCGTGTTTCTGAt                      >  1:1180263/1‑150 (MQ=255)
                                                                                                                              gCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCCGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGAt                      >  1:250678/1‑150 (MQ=255)
                                                                                                                                 tCAGTCTCAAACCGGCTCCAGATAGAGCCGGGTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCAGTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACa                   >  1:1168870/1‑150 (MQ=255)
                                                                                                                                     accaaaaCCGGCTCTATCTGGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATTCTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTc               >  1:1586409/5‑150 (MQ=255)
                                                                                                                                      ccaaaaCCGGCTCTATCTGGAGCCGGTTTTGGTTTTCTGTCTTAAAGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCa              >  1:1610878/4‑150 (MQ=255)
                                                                                                                                       tcAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAt             <  2:748673/150‑1 (MQ=255)
                                                                                                                                       caaaaCCGGCTCTATCTGGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATAGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAt             >  2:719100/3‑150 (MQ=255)
                                                                                                                                        cAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCTCGTGTTTCTGATACAGCTCATg            >  2:970585/1‑150 (MQ=255)
                                                                                                                                         aaaCCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATgg           <  1:1353294/150‑1 (MQ=255)
                                                                                                                                          aaCCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCCGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATggg          <  2:840113/150‑1 (MQ=255)
                                                                                                                                          aaCCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTAATggg          >  2:609766/1‑150 (MQ=255)
                                                                                                                                              ggCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCCAGCCCGTGTTTCTGATCCAGCTCATGGGCttt      >  2:1077486/1‑150 (MQ=255)
                                                                                                                                               gCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCCCGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTtc     >  2:1526505/1‑150 (MQ=255)
                                                                                                                                                cTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATAGAGCTCATGGGCTTtca    <  1:1606004/150‑1 (MQ=255)
                                                                                                                                                  ccAGATAGAGCCGTTTTTTGTTTTTTTTCTTATCGCTCCATGAACGGGAGCTTGTTATCTAACGTCCATAAAGGAATCAGATTCTGAATTCTAATCGGATAGTCACGCGCGCGAAGCCCGTGTTTCTGATACAGCTCATGGGCTTtcatc  <  2:308505/150‑1 (MQ=255)
                                                                                                                                                  ccAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGTATCAGATACTGCATTCCCATCGCATCCTCACTAGCGCCAAGCCCGTGTTTGTGATACCGCTCATGGGCttagatc  >  1:334412/1‑145 (MQ=255)
                                                                                                                                                  ccAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCCCGCGCGCCAAGCCCGTGTTGCTGATACAGCTCATGGGCTTtcatc  >  2:151177/1‑150 (MQ=255)
                                                                                                                                                  ccAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATAc                    <  1:339072/132‑1 (MQ=255)
                                                                                                                                                  ccAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTtcatc  >  2:1617109/1‑150 (MQ=255)
                                                                                                                                                  ccAGATAGAGCCGGTTTTGGTTTTCTGTCATAACGCACCATGCCCGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCCTTCCCATCGCATCGTCACGCGCGCCAACCCCGTGTTTCTGATACAGCTCATGGGCTTtcata  >  1:1419402/1‑149 (MQ=255)
                                                                                                                                                  ccAGATAGAGCCGGTTTTGCTTTTCTGTCTTAACGCACCATGCACGGGCGCGTGTTATCGAACGGCCAGCCAGGAATCAGATACTGCAGTCCCATCGCCTCGTCACGCGCGCCAAGCCCGTGTGTCTGATACAGCTCATGGGCTTtcatc  >  1:914724/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATC  >  NC_000913/2917870‑2918165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: