Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,918,020 A→C 9.8% intergenic (+207/+25) barA → / ← gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,918,0200AC 9.8% 376.9 / 24.6 141intergenic (+207/+25)barA/gudDhybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1
Reads supporting (aligned to +/- strand):  ref base A (73/54);  new base C (4/10);  total (77/64)
Fisher's exact test for biased strand distribution p-value = 4.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 143 total aligned reads displayed.

AGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACTT  >  NC_000913/2917873‑2918169
                                                                                                                                                   |                                                                                                                                                     
    ccTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGc                                                                                                                                                 >  1:1712823/1‑150 (MQ=255)
    ccTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGc                                                                                                                                                 <  1:752917/150‑1 (MQ=255)
     cTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGCGcc                                                                                                                                                >  2:875456/1‑150 (MQ=255)
      tgtgTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCg                                                                                                                                               >  1:531162/1‑150 (MQ=255)
        tttgtaggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCAAGATAGAGCCggt                                                                                                                                             >  2:1688563/8‑150 (MQ=38)
         ttttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtt                                                                                                                                            <  1:1661270/149‑1 (MQ=255)
         ttttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggtt                                                                                                                                            >  1:303291/2‑150 (MQ=255)
          tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttt                                                                                                                                           <  1:716812/150‑1 (MQ=255)
           ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttg                                                                                                                                          <  1:1467764/150‑2 (MQ=255)
           ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttg                                                                                                                                          <  1:946187/150‑2 (MQ=255)
           ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAAATAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttg                                                                                                                                          <  2:1197355/150‑2 (MQ=38)
             aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttgag                                                                                                                                        <  1:1600395/150‑4 (MQ=38)
             aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttgg                                                                                                                                        <  1:660552/150‑1 (MQ=255)
             aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttgg                                                                                                                                        <  1:1665144/150‑1 (MQ=255)
             aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttgg                                                                                                                                        <  1:1477958/150‑1 (MQ=255)
             aTGCCGGATGCGGCGTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggg                                                                                                                                       >  1:1398398/1‑149 (MQ=255)
              ggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGAATCAGTCTCAAACCGGCTCTATCTGGAGCCggttttggt                                                                                                                                       >  1:1549449/2‑150 (MQ=255)
                cctgATGCGGCGTTAACGCCTTATACGGCCTACAATACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTTTTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggttt                                                                                                                                     <  1:851301/147‑1 (MQ=255)
                ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttgagact                                                                                                                                     <  1:1213814/150‑7 (MQ=38)
                ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggttt                                                                                                                                     <  2:1493684/150‑1 (MQ=255)
                  ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTc                                                                                                                                   <  1:1025606/150‑1 (MQ=255)
                         gcgTTAACGCCTTATCCGGCCTACAAAACCAATTTAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACATGCTATTTTGCGTTTATCATCAGTCTCAAACCGGCACGATAAAGAGCCGGTTTTGGTTTTGTGTCttt                                                                                                                            >  2:1083403/1‑149 (MQ=38)
                          cgTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGGCCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGAATGGTTTTCTGTCTTAAc                                                                                                                          >  1:1597749/1‑150 (MQ=255)
                          cgTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACATGATACATAGAACAACAGGCTATTTTGCGTTTAGAATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGTTTTTATGTCTTaa                                                                                                                           >  2:1045443/1‑150 (MQ=255)
                              aaCGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCa                                                                                                                       >  2:42384/1‑150 (MQ=255)
                              aaCGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCa                                                                                                                       >  2:659106/1‑150 (MQ=255)
                              aaCGCCTTATCCGGCCTACAAAACAAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACACCAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGAGAGAGCCGGTTTTGGTTTTCTGTCTTAACgaa                                                                                                                       >  2:273373/1‑148 (MQ=255)
                                cGCCTTATCCGGCCTACAAAACCTATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCAcc                                                                                                                     >  2:176949/1‑150 (MQ=255)
                                cGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCAcc                                                                                                                     >  2:57417/1‑150 (MQ=255)
                                 gcattATCCGGCCTACAAAACCAATTAAATTGAATGAATTAAAAATTGATGTAGAACTGATACATAGCACAACAGGCTATTTTGTGTTTAGCATCAGTCTCAAACCGGCTCCCGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCa                                                                                                                    <  2:855005/147‑1 (MQ=255)
                                   cTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTTCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATg                                                                                                                  <  1:785396/150‑1 (MQ=255)
                                   cTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATg                                                                                                                  >  2:1606004/1‑150 (MQ=255)
                                     tATGCGGCCTACAATACCAATTAAATTCAATGAATTAAAAAATGTTGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCa                                                                                                                <  2:190792/150‑1 (MQ=255)
                                     tATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCa                                                                                                                >  2:23924/1‑150 (MQ=255)
                                       tCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACg                                                                                                              >  1:1028526/1‑150 (MQ=255)
                                             cTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGAGCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCGGGTTTTGGTTTTCTGTCTTAACGCACCCTGCAcgcgcgat                                                                                                        >  2:93597/1‑148 (MQ=255)
                                                   aaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTAt                                                                                                  <  1:1458023/150‑1 (MQ=255)
                                                    aCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCGATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATc                                                                                                 <  1:207087/150‑1 (MQ=255)
                                                     caaaTTAAATTCAATTAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCg                                                                                                <  2:666264/148‑1 (MQ=255)
                                                      cAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTGTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGa                                                                                               >  2:242292/1‑150 (MQ=255)
                                                        aTTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCTGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAAc                                                                                             >  1:208017/1‑150 (MQ=255)
                                                         ttAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACg                                                                                            <  2:766184/150‑1 (MQ=255)
                                                            aattaaattaATTAAATAATGATGTAGTCCTGATACATAGCAGTACATGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTcc                                                                                         <  2:1567863/150‑1 (MQ=255)
                                                            aaTTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACTTcc                                                                                         <  2:873024/150‑1 (MQ=255)
                                                             aTTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTcca                                                                                        >  2:1330019/1‑150 (MQ=255)
                                                                cAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTccagcc                                                                                     <  1:1411718/150‑1 (MQ=255)
                                                                  aTGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGGTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTccagccag                                                                                   <  2:1594899/150‑1 (MQ=255)
                                                                    gAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGCGCCGGTTTTGTTTTTCTGTATTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCAAGGa                                                                                 >  1:1280628/1‑150 (MQ=255)
                                                                    gAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGa                                                                                 >  2:626845/1‑150 (MQ=255)
                                                                     aaTTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGat                                                                                >  1:1217169/1‑149 (MQ=255)
                                                                     aaTTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGaa                                                                                <  1:395569/150‑1 (MQ=255)
                                                                     aaTTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGaa                                                                                <  2:862090/150‑1 (MQ=255)
                                                                       ttAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATc                                                                              >  2:1086435/1‑150 (MQ=255)
                                                                        tAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCa                                                                             <  1:292117/150‑1 (MQ=255)
                                                                            aaaTGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATa                                                                         >  2:409872/1‑150 (MQ=255)
                                                                              atgatgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACt                                                                       <  1:42384/150‑1 (MQ=255)
                                                                              atgatgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGAATCAGTCTCAAACAGGCTCCAGATAGCGCCGGTTTAGGTATTCTGTCTTAACGCACCAAGCACTGGCGCATGTTATCGAAAGTCCATCCAGGAATCAGATTCt                                                                       >  1:1266486/1‑150 (MQ=255)
                                                                                gatgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGGCTCACAGCGGCTCCAGATAGATCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGc                                                                     <  1:277732/150‑1 (MQ=255)
                                                                                    tAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGAACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTc                                                                 >  1:851934/1‑150 (MQ=255)
                                                                                     aGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATTAGATACTGCATTcc                                                                <  2:303291/150‑1 (MQ=255)
                                                                                        ccagATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACTCACCATCCACGGTCGCTTGTTATCGAACCTCCAGCCAGGAATCAGAAACTGCATTCCcat                                                             >  2:1327343/4‑150 (MQ=255)
                                                                                         cTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCcatc                                                            <  1:1381203/150‑1 (MQ=255)
                                                                                          tGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCcatcg                                                           >  1:1307126/1‑150 (MQ=255)
                                                                                             tACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGCGCCGGTTTTGGTTTTCTGTCTTAACGCCCCATGCCCGGGCGCTTGTTATAGAACGTCCAGCCAGGAATCATATACTGCATTCCcatcgcat                                                        >  2:1456942/1‑150 (MQ=255)
                                                                                             tACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCCCGGGCGCTTGGTATCGAACGTCCAGCCCGGCATCACAGACTGCATTACcatcgcct                                                        >  1:1544699/1‑148 (MQ=255)
                                                                                             tACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGCTACTGCATTCCcatcgccg                                                        >  2:1635810/1‑148 (MQ=255)
                                                                                               cATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCcatcgcatcg                                                      <  1:1086435/150‑1 (MQ=255)
                                                                                               cATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCcatcgcatcg                                                      >  1:1114592/1‑150 (MQ=255)
                                                                                               cATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGGTTTGGTTTTCTGTCTTAACGCACCATTCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCCTTCCcatcgcatag                                                      >  2:734019/1‑148 (MQ=255)
                                                                                                aTAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGt                                                     >  1:342811/1‑150 (MQ=255)
                                                                                                aTAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTACAGCCAGGAATCAGATACTGCATTCCCATCGCATCGt                                                     >  1:1443390/1‑150 (MQ=255)
                                                                                                 tAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTc                                                    <  1:659106/150‑1 (MQ=255)
                                                                                                  aGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCa                                                   <  1:171327/150‑1 (MQ=255)
                                                                                                      caacaGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATAGCATCGTCAcgcg                                               >  2:783172/1‑150 (MQ=255)
                                                                                                           ggCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCa                                          >  1:995621/1‑150 (MQ=255)
                                                                                                                 tttGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCg                                    <  1:1324687/150‑1 (MQ=255)
                                                                                                                        ttAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCt                             >  2:1533950/1‑150 (MQ=255)
                                                                                                                          aGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGa                           <  1:1381394/150‑1 (MQ=255)
                                                                                                                          aGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGGTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTGCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGAGCGCCACGCCCGTGTGTCTgg                           >  1:329518/1‑149 (MQ=255)
                                                                                                                           gCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGAt                          <  1:1136712/150‑1 (MQ=255)
                                                                                                                           gCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGAt                          <  2:1712823/150‑1 (MQ=255)
                                                                                                                           gCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATAGTCACGCGCGCCAAGCCCGTGTTTCTGAt                          >  1:1180263/1‑150 (MQ=255)
                                                                                                                           gCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCCGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGAt                          >  1:250678/1‑150 (MQ=255)
                                                                                                                              tCAGTCTCAAACCGGCTCCAGATAGAGCCGGGTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCAGTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACa                       >  1:1168870/1‑150 (MQ=255)
                                                                                                                                   ccaaaaCCGGCTCTATCTGGAGCCGGTTTTGGTTTTCTGTCTTAAAGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCa                  >  1:1610878/4‑150 (MQ=255)
                                                                                                                                    tcAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAt                 <  2:748673/150‑1 (MQ=255)
                                                                                                                                    caaaaCCGGCTCTATCTGGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATAGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAt                 >  2:719100/3‑150 (MQ=255)
                                                                                                                                     cAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCTCGTGTTTCTGATACAGCTCATg                >  2:970585/1‑150 (MQ=255)
                                                                                                                                      aaaCCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATgg               <  1:1353294/150‑1 (MQ=255)
                                                                                                                                       aaCCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTAATggg              >  2:609766/1‑150 (MQ=255)
                                                                                                                                           ggCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCCAGCCCGTGTTTCTGATCCAGCTCATGGGCttt          >  2:1077486/1‑150 (MQ=255)
                                                                                                                                            gCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCCCGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTtc         >  2:1526505/1‑150 (MQ=255)
                                                                                                                                               ccAGATAGAGCCGTTTTTTGTTTTTTTTCTTATCGCTCCATGAACGGGAGCTTGTTATCTAACGTCCATAAAGGAATCAGATTCTGAATTCTAATCGGATAGTCACGCGCGCGAAGCCCGTGTTTCTGATACAGCTCATGGGCTTtcatc      <  2:308505/150‑1 (MQ=255)
                                                                                                                                               ccAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCCCGCGCGCCAAGCCCGTGTTGCTGATACAGCTCATGGGCTTtcatc      >  2:151177/1‑150 (MQ=255)
                                                                                                                                               ccAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATAc                        <  1:339072/132‑1 (MQ=255)
                                                                                                                                               ccAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTtcatc      >  2:1617109/1‑150 (MQ=255)
                                                                                                                                               ccAGATAGAGCCGGTTTTGGTTTTCTGTCATAACGCACCATGCCCGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCCTTCCCATCGCATCGTCACGCGCGCCAACCCCGTGTTTCTGATACAGCTCATGGGCTTtcata      >  1:1419402/1‑149 (MQ=255)
                                                                                                                                               ccAGATAGAGCCGGTTTTGCTTTTCTGTCTTAACGCACCATGCACGGGCGCGTGTTATCGAACGGCCAGCCAGGAATCAGATACTGCAGTCCCATCGCCTCGTCACGCGCGCCAAGCCCGTGTGTCTGATACAGCTCATGGGCTTtcatc      >  1:914724/1‑150 (MQ=255)
                                                                                                                                                  taTAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACt   <  2:1168584/149‑1 (MQ=255)
                                                                                                                                                   aTAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACtt  <  1:1160197/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACTT  >  NC_000913/2917873‑2918169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: