Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,918,022 A→G 9.9% intergenic (+209/+23) barA → / ← gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,918,0220AG 9.9% 372.9 / 25.4 141intergenic (+209/+23)barA/gudDhybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1
Reads supporting (aligned to +/- strand):  ref base A (72/55);  new base G (4/10);  total (76/65)
Fisher's exact test for biased strand distribution p-value = 5.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

Only 100 of 145 total aligned reads displayed.

AGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACTTGA  >  NC_000913/2917873‑2918171
                                                                                                                                                     |                                                                                                                                                     
aGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATa                                                                                                                                                       <  2:1064262/150‑1 (MQ=255)
  tGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATaga                                                                                                                                                     >  1:1419112/1‑150 (MQ=255)
    ccTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGc                                                                                                                                                   >  1:1712823/1‑150 (MQ=255)
    ccTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGc                                                                                                                                                   <  1:752917/150‑1 (MQ=255)
     cTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGCGcc                                                                                                                                                  >  2:875456/1‑150 (MQ=255)
      tgtgTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCg                                                                                                                                                 >  1:531162/1‑150 (MQ=255)
          tttATGCCGGATGCGGCGTTAACGCCTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttg                                                                                                                                            <  2:737239/150‑2 (MQ=39)
           ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttg                                                                                                                                            <  1:1467764/150‑2 (MQ=255)
           ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttg                                                                                                                                            <  1:664293/150‑2 (MQ=255)
           ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttg                                                                                                                                            <  1:946187/150‑2 (MQ=255)
             aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttgg                                                                                                                                          <  1:660552/150‑1 (MQ=255)
             aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttgg                                                                                                                                          <  1:1665144/150‑1 (MQ=255)
             aTGCCGGATGCGGCGTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggg                                                                                                                                         >  1:1398398/1‑149 (MQ=255)
              tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttgaga                                                                                                                                         <  1:30055/150‑5 (MQ=38)
                cctgATGCGGCGTTAACGCCTTATACGGCCTACAATACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTTTTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggttt                                                                                                                                       <  1:851301/147‑1 (MQ=255)
                ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttgagact                                                                                                                                       <  1:1213814/150‑7 (MQ=38)
                ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggttt                                                                                                                                       <  2:1493684/150‑1 (MQ=255)
                 cggttaaggcgTTAACGCCGCATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGCCCTGATACATAGCACAACAGGCTATTTTGCGGTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggtttt                                                                                                                                      >  1:1244643/8‑150 (MQ=255)
                 cGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggtttt                                                                                                                                      >  1:984527/1‑150 (MQ=255)
                 cGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCACTCTCAAACCGGCTCCAGATAGAGCCggttttggtttt                                                                                                                                      >  2:1597961/1‑150 (MQ=255)
                  ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTc                                                                                                                                     <  1:1025606/150‑1 (MQ=255)
                  ggATGCGGCGTTAACGCCTTACCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTc                                                                                                                                     <  1:358495/150‑1 (MQ=255)
                         gcgTTAACGCCTTATCCGGCCTACAAAACCAATTTAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACATGCTATTTTGCGTTTATCATCAGTCTCAAACCGGCACGATAAAGAGCCGGTTTTGGTTTTGTGTCttt                                                                                                                              >  2:1083403/1‑149 (MQ=38)
                          cgTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGGCCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGAATGGTTTTCTGTCTTAAc                                                                                                                            >  1:1597749/1‑150 (MQ=255)
                          cgTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACATGATACATAGAACAACAGGCTATTTTGCGTTTAGAATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGTTTTTATGTCTTaa                                                                                                                             >  2:1045443/1‑150 (MQ=255)
                              aaCGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCa                                                                                                                         >  2:42384/1‑150 (MQ=255)
                              aaCGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCa                                                                                                                         >  2:659106/1‑150 (MQ=255)
                              aaCGCCTTATCCGGCCTACAAAACAAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACACCAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGAGAGAGCCGGTTTTGGTTTTCTGTCTTAACgaa                                                                                                                         >  2:273373/1‑148 (MQ=255)
                                cGCCTTATCCGGCCTACAAAACCTATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCAcc                                                                                                                       >  2:171327/1‑150 (MQ=255)
                                 gcattATCCGGCCTACAAAACCAATTAAATTGAATGAATTAAAAATTGATGTAGAACTGATACATAGCACAACAGGCTATTTTGTGTTTAGCATCAGTCTCAAACCGGCTCCCGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCa                                                                                                                      <  2:855005/147‑1 (MQ=255)
                                   cTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATg                                                                                                                    >  2:1606004/1‑150 (MQ=255)
                                     tATGCGGCCTACAATACCAATTAAATTCAATGAATTAAAAAATGTTGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCa                                                                                                                  <  2:190792/150‑1 (MQ=255)
                                     tATCAGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCa                                                                                                                  <  1:248965/150‑1 (MQ=255)
                                       tCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACg                                                                                                                >  1:1028526/1‑150 (MQ=255)
                                             cTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGAGCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCGGGTTTTGGTTTTCTGTCTTAACGCACCCTGCAcgcgcgat                                                                                                          >  2:93597/1‑148 (MQ=255)
                                             cTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCt                                                                                                          <  1:1388382/150‑1 (MQ=255)
                                                  aaaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTa                                                                                                     >  2:1521379/1‑150 (MQ=255)
                                                   aaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTAt                                                                                                    <  1:1458023/150‑1 (MQ=255)
                                                    aCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCGATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATc                                                                                                   <  1:207087/150‑1 (MQ=255)
                                                     caaaTTAAATTCAATTAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCg                                                                                                  <  2:666264/148‑1 (MQ=255)
                                                      cAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTGTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGa                                                                                                 >  2:242292/1‑150 (MQ=255)
                                                         ttAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACg                                                                                              <  1:1694182/150‑1 (MQ=255)
                                                         ttAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACg                                                                                              <  2:766184/150‑1 (MQ=255)
                                                         ttAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACg                                                                                              <  2:234583/150‑1 (MQ=255)
                                                            aaTTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACTTcc                                                                                           <  2:873024/150‑1 (MQ=255)
                                                             aTTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTcca                                                                                          >  2:1330019/1‑150 (MQ=255)
                                                                  aTGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGGTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTccagccag                                                                                     <  2:1594899/150‑1 (MQ=255)
                                                                    gAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGCGCCGGTTTTGTTTTTCTGTATTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCAAGGa                                                                                   >  1:1280628/1‑150 (MQ=255)
                                                                    gAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGa                                                                                   >  2:626845/1‑150 (MQ=255)
                                                                     aaTTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGaa                                                                                  <  1:395569/150‑1 (MQ=255)
                                                                     aaTTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGaa                                                                                  <  2:862090/150‑1 (MQ=255)
                                                                       ttAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAAta                                                                                >  1:154177/1‑149 (MQ=255)
                                                                       ttAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATc                                                                                >  2:1086435/1‑150 (MQ=255)
                                                                        tAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCa                                                                               <  1:292117/150‑1 (MQ=255)
                                                                            aaaTGATGTAGACCTGATACATAGCACAACACTCTATTTTGCGTTTAGCATCCGTCTCACACCGGCTCCAGATAGCGCCGGGTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATa                                                                           <  1:853588/150‑1 (MQ=255)
                                                                              atgatgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACt                                                                         >  1:595846/1‑150 (MQ=255)
                                                                              atgatgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGAATCAGTCTCAAACAGGCTCCAGATAGCGCCGGTTTAGGTATTCTGTCTTAACGCACCAAGCACTGGCGCATGTTATCGAAAGTCCATCCAGGAATCAGATTCt                                                                         >  1:1266486/1‑150 (MQ=255)
                                                                                 atgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCa                                                                      >  1:1448332/1‑150 (MQ=255)
                                                                                    tAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGAACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTc                                                                   >  1:851934/1‑150 (MQ=255)
                                                                                     aGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATTAGATACTGCATTcc                                                                  <  2:303291/150‑1 (MQ=255)
                                                                                      gACCTGATACATAGCACAACAGGCTATTTTGCGATTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCCCCATGCACGGGCGCTTGTTATAGAACGTCCAGCCAGGAATCAGATACTGCATAccc                                                                 >  2:1660693/1‑150 (MQ=255)
                                                                                        ccagATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACTCACCATCCACGGTCGCTTGTTATCGAACCTCCAGCCAGGAATCAGAAACTGCATTCCcat                                                               >  2:1327343/4‑150 (MQ=255)
                                                                                         cTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCcatc                                                              <  1:1381203/150‑1 (MQ=255)
                                                                                          tGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCcatcg                                                             >  1:1307126/1‑150 (MQ=255)
                                                                                           gATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCCTTCCcatcgc                                                            >  2:1204080/1‑150 (MQ=255)
                                                                                             tACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGCGCCGGTTTTGGTTTTCTGTCTTAACGCCCCATGCCCGGGCGCTTGTTATAGAACGTCCAGCCAGGAATCATATACTGCATTCCcatcgcat                                                          >  2:1456942/1‑150 (MQ=255)
                                                                                             tACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCCCGGGCGCTTGGTATCGAACGTCCAGCCCGGCATCACAGACTGCATTACcatcgcct                                                          >  1:1544699/1‑148 (MQ=255)
                                                                                             tACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGCTACTGCATTCCcatcgccg                                                          >  2:1635810/1‑148 (MQ=255)
                                                                                               cATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCcatcgcatcg                                                        >  1:1114592/1‑150 (MQ=255)
                                                                                               cATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCcatcgcatcg                                                        <  1:1086435/150‑1 (MQ=255)
                                                                                               cATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGGTTTGGTTTTCTGTCTTAACGCACCATTCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCCTTCCcatcgcatag                                                        >  2:734019/1‑148 (MQ=255)
                                                                                                aTAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGt                                                       >  1:342811/1‑150 (MQ=255)
                                                                                                  aGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCa                                                     <  1:176949/150‑1 (MQ=255)
                                                                                                   gCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCAc                                                    <  2:572869/150‑1 (MQ=255)
                                                                                                      caacaGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATAGCATCGTCAcgcg                                                 >  2:783172/1‑150 (MQ=255)
                                                                                                                 tttGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCg                                      <  1:1324687/150‑1 (MQ=255)
                                                                                                                       tttAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGGTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTc                                >  1:770631/1‑150 (MQ=255)
                                                                                                                        ttAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCt                               >  2:1533950/1‑150 (MQ=255)
                                                                                                                          aGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGGTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTGCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGAGCGCCACGCCCGTGTGTCTgg                             >  1:329518/1‑149 (MQ=255)
                                                                                                                           gCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGAt                            <  2:1712823/150‑1 (MQ=255)
                                                                                                                           gCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGAt                            <  1:1136712/150‑1 (MQ=255)
                                                                                                                           gCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATAGTCACGCGCGCCAAGCCCGTGTTTCTGAt                            >  1:1180263/1‑150 (MQ=255)
                                                                                                                           gCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCCGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGAt                            >  1:250678/1‑150 (MQ=255)
                                                                                                                              tCAGTCTCAAACCGGCTCCAGATAGAGCCGGGTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCAGTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACa                         >  1:1168870/1‑150 (MQ=255)
                                                                                                                               cAGTCTCAAACCCGCTCTATCTGGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAg                        <  2:1549449/150‑1 (MQ=255)
                                                                                                                                  accaaaaCCGGCTCTATCTGGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATTCTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTc                     >  1:1586409/5‑150 (MQ=255)
                                                                                                                                   ccaaaaCCGGCTCTATCTGGAGCCGGTTTTGGTTTTCTGTCTTAAAGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCa                    >  1:1610878/4‑150 (MQ=255)
                                                                                                                                    caaaaCCGGCTCTATCTGGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATAGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAt                   >  2:719100/3‑150 (MQ=255)
                                                                                                                                     cAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCTCGTGTTTCTGATACAGCTCATg                  >  2:970585/1‑150 (MQ=255)
                                                                                                                                      aaaCCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATgg                 <  1:1353294/150‑1 (MQ=255)
                                                                                                                                       aaCCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTAATggg                >  2:609766/1‑150 (MQ=255)
                                                                                                                                             cTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATAGAGCTCATGGGCTTtca          <  1:1606004/150‑1 (MQ=255)
                                                                                                                                               ccAGATAGAGCCGTTTTTTGTTTTTTTTCTTATCGCTCCATGAACGGGAGCTTGTTATCTAACGTCCATAAAGGAATCAGATTCTGAATTCTAATCGGATAGTCACGCGCGCGAAGCCCGTGTTTCTGATACAGCTCATGGGCTTtcatc        <  2:308505/150‑1 (MQ=255)
                                                                                                                                               ccAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGTATCAGATACTGCATTCCCATCGCATCCTCACTAGCGCCAAGCCCGTGTTTGTGATACCGCTCATGGGCttagatc        >  1:334412/1‑145 (MQ=255)
                                                                                                                                               ccAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCCCGCGCGCCAAGCCCGTGTTGCTGATACAGCTCATGGGCTTtcatc        >  2:151177/1‑150 (MQ=255)
                                                                                                                                               ccAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATAc                          >  2:339072/1‑132 (MQ=255)
                                                                                                                                               ccAGATAGAGCCGGTTTTGGTTTTCTGTCATAACGCACCATGCCCGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCCTTCCCATCGCATCGTCACGCGCGCCAACCCCGTGTTTCTGATACAGCTCATGGGCTTtcata        >  1:1419402/1‑149 (MQ=255)
                                                                                                                                                  taTAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACt     <  2:1168584/149‑1 (MQ=255)
                                                                                                                                                   aTAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACtt    <  1:1160197/150‑1 (MQ=255)
                                                                                                                                                     agagCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCCTTCCGATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACTTgg  >  2:1145338/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACTTGA  >  NC_000913/2917873‑2918171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: