Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,939,335 C→T 12.1% intergenic (+25/‑33) fucU → / → fucR L‑fucose mutarotase/l‑fucose operon activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,939,3350CT12.1% 269.7 / 21.3 99intergenic (+25/‑33)fucU/fucRL‑fucose mutarotase/l‑fucose operon activator
Reads supporting (aligned to +/- strand):  ref base C (39/48);  new base T (9/3);  total (48/51)
Fisher's exact test for biased strand distribution p-value = 6.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

Only 100 of 102 total aligned reads displayed.

TGACATCATCCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGCGATCTC  >  NC_000913/2939187‑2939484
                                                                                                                                                    |                                                                                                                                                     
tGACATCATCCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGAcg                                                                                                                                                      <  2:431411/150‑1 (MQ=255)
  aCATCATCCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGAcgcg                                                                                                                                                    <  1:1012067/150‑1 (MQ=255)
   catcatCCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACgcgg                                                                                                                                                   >  1:728622/1‑150 (MQ=255)
       atcCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCGTCATgcgggcgt                                                                                                                                               <  1:1270100/150‑2 (MQ=255)
       atcCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACgcgggcgg                                                                                                                                               <  1:1598210/150‑1 (MQ=255)
        tcCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGt                                                                                                                                              <  1:1718742/150‑1 (MQ=255)
        tcCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATAGTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGt                                                                                                                                              >  1:696060/1‑150 (MQ=255)
          cGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCGTCATgcgggcgtacc                                                                                                                                            <  1:1000086/150‑5 (MQ=255)
          cGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCGTCATgcgggcgtacc                                                                                                                                            <  1:211059/150‑5 (MQ=255)
             aTCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCg                                                                                                                                         <  1:838887/150‑1 (MQ=255)
             aTCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCg                                                                                                                                         <  1:752655/150‑1 (MQ=255)
                aaTCGTTTTGCGTTTTATGAACGGGCGCAAAACGCATTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAat                                                                                                                                      >  2:651638/1‑150 (MQ=255)
                aaTCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACTGGTACGCCCGCATGACGCGGGCGGTTATCGAat                                                                                                                                      <  1:1500109/150‑1 (MQ=255)
                aaTCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAat                                                                                                                                      >  1:1419904/1‑150 (MQ=255)
                aaTCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTGTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTGTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAat                                                                                                                                      >  1:457127/1‑150 (MQ=255)
                    gTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAatgatg                                                                                                                                  >  2:1068426/1‑150 (MQ=255)
                     ttttGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATgg                                                                                                                                 <  1:624076/150‑1 (MQ=255)
                     ttttGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATgg                                                                                                                                 <  2:45289/150‑1 (MQ=255)
                      tttGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATggg                                                                                                                                <  2:728622/150‑1 (MQ=255)
                      tttGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATggg                                                                                                                                <  1:683739/150‑1 (MQ=255)
                       ttGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATgggg                                                                                                                               <  1:1613328/150‑1 (MQ=255)
                             tttATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGaaaa                                                                                                                         >  2:623507/1‑150 (MQ=255)
                               tATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAat                                                                                                                       <  2:1665255/150‑1 (MQ=255)
                               tATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAat                                                                                                                       >  1:437529/1‑150 (MQ=255)
                                ttGAACGGGCGCAAAAAGCCTTTGCGATAGTTATCACCGGGGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAata                                                                                                                      <  2:834743/149‑1 (MQ=255)
                                aTGAACGGGCGCAAAAAGGCTTTGCGAGCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAata                                                                                                                      <  2:163626/150‑1 (MQ=255)
                                 ttaaCGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAatat                                                                                                                     <  1:599015/148‑1 (MQ=255)
                                 tGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTTAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAatat                                                                                                                     <  1:1410862/150‑1 (MQ=255)
                                    aCGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGaa                                                                                                                  <  2:683459/150‑1 (MQ=255)
                                      gtgcgcAAAAAGCCTTTGCGATCGTTATCACAGGCCAACGAGCTAAGTACGGGAATATTCTTTTAAAAAAATGGGTAACACAGTAATCTCATACCGGTACGCCCGCATGCCGAGGGCGGTTATCGAATGATGGGGTGAAAAATATCaaac                                                                                                                >  2:770113/3‑149 (MQ=255)
                                       gacgcAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGc                                                                                                               >  1:1193024/3‑150 (MQ=255)
                                        gcgcAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCg                                                                                                              <  2:812959/150‑1 (MQ=255)
                                         cgcAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCTTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCgg                                                                                                             <  1:722920/150‑1 (MQ=255)
                                         cgcAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGTTGGGGTGAAAAATATGAAAGCgg                                                                                                             <  1:230267/150‑1 (MQ=255)
                                         cgcAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCgg                                                                                                             <  1:915665/150‑1 (MQ=255)
                                          gcAACAAGCCTTTGCGATCGTTATCACAGGCGAACGAGAGAAGTACTGGTATATTCTTTTAAAAAAAGGGGCCACCCCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGc                                                                                                            <  2:725584/150‑1 (MQ=255)
                                               aaGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGcc                                                                                                       <  2:1545855/150‑1 (MQ=255)
                                                 gCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAg                                                                                                     <  1:1080737/150‑1 (MQ=255)
                                                    tttGCGATCGTTATCACAGTCGATCGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTTACACAGTAATATCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAATAATATGAAAGCGGCACGCCAGCaa                                                                                                  >  2:708222/1‑150 (MQ=255)
                                                     ttGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAg                                                                                                 <  2:1391916/150‑1 (MQ=255)
                                                     ttGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGAAACACCGTAATCTCATCCCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGGAAAAATATGAAAGCGGCACGCCAGCAAg                                                                                                 <  2:1656744/150‑1 (MQ=255)
                                                      tGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGc                                                                                                <  2:1339806/150‑1 (MQ=255)
                                                        cGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGa                                                                                              >  1:273593/1‑150 (MQ=255)
                                                        cGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGAGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGa                                                                                              >  2:115288/1‑150 (MQ=255)
                                                             gTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCg                                                                                        >  2:335871/1‑150 (MQ=255)
                                                               tATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGa                                                                                       <  2:701774/150‑1 (MQ=255)
                                                                aTCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGAc                                                                                      >  2:828584/1‑150 (MQ=255)
                                                                 tCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTCACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGAcc                                                                                     <  2:1320979/150‑1 (MQ=255)
                                                                 tCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGAcc                                                                                     <  1:1348773/150‑1 (MQ=255)
                                                                 tCACAGGCGAACGAGCGAAGTAAGGGAATATTCTTTTAAAAAAAGGGGTAAAACCGTAATCTAATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGAcc                                                                                     <  2:122111/150‑1 (MQ=255)
                                                                  cacaGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGAAACACAGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTAATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACct                                                                                    >  2:679173/1‑150 (MQ=255)
                                                                   acaGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCCCGCCAGCAAGCGATAGTCGACctg                                                                                   >  1:1290485/1‑150 (MQ=255)
                                                                      ggCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACctgctg                                                                                >  1:622926/1‑150 (MQ=255)
                                                                        cGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACctgctgct                                                                              <  1:1091333/150‑1 (MQ=255)
                                                                          aaCGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGa                                                                            >  2:1660899/1‑150 (MQ=255)
                                                                                 gAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATAc                                                                     >  1:115428/1‑150 (MQ=255)
                                                                                      aCGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGcc                                                                <  2:488320/150‑1 (MQ=255)
                                                                                      aCGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGcc                                                                <  2:140156/150‑1 (MQ=255)
                                                                                        gggAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTg                                                              <  1:473495/150‑1 (MQ=255)
                                                                                         ggAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGa                                                             >  2:806537/1‑150 (MQ=255)
                                                                                         ggAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGa                                                             >  1:1194924/1‑150 (MQ=255)
                                                                                         ggAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGa                                                             >  1:762409/1‑150 (MQ=255)
                                                                                             tatTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCAc                                                         >  1:628829/1‑150 (MQ=255)
                                                                                               ttCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTCCTGCTGAACCATACCAGCCTGACCACgg                                                       >  2:1034729/1‑150 (MQ=255)
                                                                                                 cTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGaa                                                     >  1:706194/1‑150 (MQ=255)
                                                                                                 cTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGaa                                                     >  1:1654769/1‑150 (MQ=255)
                                                                                                 cTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTAAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGaa                                                     >  1:1124056/1‑150 (MQ=255)
                                                                                                  ttttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAg                                                    <  1:1050435/150‑1 (MQ=255)
                                                                                                  ttttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAg                                                    >  1:1573418/1‑150 (MQ=255)
                                                                                                  ttttAAAAAAAGGGGTAACACCGTAATCTCAAACCGGTACGCCCGGATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCAGCTGAACCATACCAGCCTGCCCACGGAAg                                                    >  2:56092/1‑150 (MQ=255)
                                                                                                   tttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGc                                                   <  1:47912/150‑1 (MQ=255)
                                                                                                       aaaaaaGGGGTAACACCGTAATCTCATACCGTTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACATGCTGCTGAACAATACCAGCCTGACCACGGAAGctctc                                               >  1:1168143/1‑150 (MQ=255)
                                                                                                            aGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGa                                          <  2:1486143/150‑1 (MQ=255)
                                                                                                             ggggTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGaa                                         <  2:1478918/150‑1 (MQ=255)
                                                                                                             ggggTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGaa                                         >  2:622656/1‑150 (MQ=255)
                                                                                                                gTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGACTGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAg                                      <  1:889138/150‑1 (MQ=255)
                                                                                                                    cacCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCCGCCTGACCACGGAAGCTCTCTCTGAACAGCTaa                                  >  2:153409/1‑150 (MQ=255)
                                                                                                                     acCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTaaa                                 >  1:1169403/1‑150 (MQ=255)
                                                                                                                      ccGTAATCTCATACCTGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATAGGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACAACGGAAGCTCTCTCTGAACAGCTAAAg                                <  2:1031474/150‑1 (MQ=255)
                                                                                                                       cGTAATCTCATACCTGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAgg                               <  2:25373/150‑1 (MQ=255)
                                                                                                                         tAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTc                             <  1:1310201/150‑1 (MQ=255)
                                                                                                                           aTCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAg                           <  2:273593/150‑1 (MQ=255)
                                                                                                                               cATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAACAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGCACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTaaa                       >  1:71959/1‑150 (MQ=255)
                                                                                                                               cATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTaaa                       >  1:627326/1‑150 (MQ=255)
                                                                                                                                 tACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGa                     <  1:623507/150‑1 (MQ=255)
                                                                                                                                  cgataaccGCCCGCGTCATGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGaa                    >  2:725072/8‑150 (MQ=255)
                                                                                                                                   gataaccGCCCGCGTCATGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGCCCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGaaa                   >  2:813208/7‑150 (MQ=255)
                                                                                                                                   gataaccGCCCGCGTCATGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGaaa                   >  2:539961/7‑150 (MQ=255)
                                                                                                                                   ccGGTACGCCAGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGCCCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCGCTCTCTGCACAGCTAAAGGTCAGTAAAGaaa                   >  1:1593894/1‑150 (MQ=255)
                                                                                                                                     taaccGCCCGCGTCATGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCCCGGAAGCTCTCGCTGAACAGCTAAAGGTCAGTAAAGAAAcc                 >  1:1283267/5‑150 (MQ=255)
                                                                                                                                      aaccGCCCGCGTCATGCGGGCGGTTATCGAATGGTGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCATGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCa                >  2:831669/4‑150 (MQ=255)
                                                                                                                                      aaccGCCCGCGTCATGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCa                >  1:1492357/4‑150 (MQ=255)
                                                                                                                                       accGCCCGCGTCATGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCCGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCAt               >  2:1130270/3‑150 (MQ=255)
                                                                                                                                       accGCCCGCGTCATGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCAt               >  1:971159/3‑150 (MQ=255)
                                                                                                                                       accGCCCGCGTCATGCGGGCGGTTATCGAATCATGGGGTGAAAAATATGAAAGCGGCACGCCCGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAACCCAt               >  2:243812/3‑150 (MQ=255)
                                                                                                                                          gCCCGCATGACGTGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAGGTCAGTAAAGAAACCATtcgt           >  2:789234/1‑150 (MQ=255)
                                                                                                                                          gCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATtcg            <  1:1245594/150‑1 (MQ=255)
                                                                                                                                                 tGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGCGAt     >  1:563115/1‑150 (MQ=255)
                                                                                                                                                 tGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGCGAt     <  2:437529/150‑1 (MQ=255)
                                                                                                                                                    cgcgGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGCGAtctc  <  1:828584/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGACATCATCCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGCGATCTC  >  NC_000913/2939187‑2939484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: