Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,971,146 T→C 7.6% intergenic (+13/‑125) ygdQ → / → ygdR UPF0053 family inner membrane protein/DUF903 family verified lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,971,1460TC 7.6% 416.4 / 17.6 145intergenic (+13/‑125)ygdQ/ygdRUPF0053 family inner membrane protein/DUF903 family verified lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (64/69);  new base C (6/5);  total (70/75)
Fisher's exact test for biased strand distribution p-value = 7.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

ATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATA  >  NC_000913/2970999‑2971291
                                                                                                                                                   |                                                                                                                                                 
aTTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCTAGTTGATTCGCAACAAAAAGAATGCGCTCTGATACTCCGTACGctct                                                                                                                                                 >  2:941838/1‑150 (MQ=255)
  tCTGGTGGGCTTTACCCTGATTCTGTAAAGTTTCGACTTCCACGTACGGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTTCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTcc                                                                                                                                               <  2:40584/150‑1 (MQ=255)
  tCTGGTGGGCTTTACCCTGATTCTGGAAAGTTGCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTcc                                                                                                                                               <  2:1122539/150‑1 (MQ=255)
      gtggGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCg                                                                                                                                           >  1:336685/1‑150 (MQ=255)
        gggCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCggg                                                                                                                                         <  1:413625/150‑1 (MQ=255)
          gCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCTAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAg                                                                                                                                       <  2:1476943/150‑1 (MQ=255)
             ttACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGc                                                                                                                                    >  1:383616/1‑150 (MQ=255)
                cccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCg                                                                                                                                           <  2:1443698/140‑1 (MQ=255)
                 ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTac                                                                                                                                >  2:1262545/1‑150 (MQ=255)
                 ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCCGCAGGAGAGCGTac                                                                                                                                <  1:952220/150‑1 (MQ=255)
                 ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                                                                <  2:1665627/150‑1 (MQ=255)
                 ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                                                                <  1:228501/150‑1 (MQ=255)
                 ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                                                                <  1:1653183/150‑1 (MQ=255)
                   tGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactc                                                                                                                              <  1:732459/150‑1 (MQ=255)
                    gATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactca                                                                                                                             <  1:486318/150‑1 (MQ=255)
                      ttCTGGAAAGTTTCGACAACCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcact                                                                                                                           <  2:119632/150‑1 (MQ=255)
                       tCTGGACAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcactc                                                                                                                          <  2:1288270/150‑1 (MQ=255)
                        cTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                                                                         >  1:198449/1‑150 (MQ=255)
                         tGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCt                                                                                                                        <  2:359859/150‑1 (MQ=255)
                         tGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCt                                                                                                                        <  2:762440/150‑1 (MQ=255)
                          ggAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtt                                                                                                                       >  1:366893/1‑150 (MQ=255)
                          ggAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtt                                                                                                                       >  1:1588124/1‑150 (MQ=255)
                           gAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCttt                                                                                                                      <  2:1427490/150‑1 (MQ=255)
                            aaaGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtttt                                                                                                                     <  1:544767/150‑1 (MQ=255)
                            aaaGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtttt                                                                                                                     >  2:549295/1‑150 (MQ=255)
                               gTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAcc                                                                                                                  >  1:1179188/1‑150 (MQ=255)
                                 ttCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCt                                                                                                                >  1:1538944/1‑150 (MQ=255)
                                    gACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCAGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTcac                                                                                                             >  1:255785/1‑150 (MQ=255)
                                      cATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAg                                                                                                           >  1:21693/1‑150 (MQ=255)
                                      cATCCACGTAACGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAg                                                                                                           <  2:457212/150‑1 (MQ=255)
                                         ccACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGttt                                                                                                        >  2:554623/1‑150 (MQ=255)
                                          cACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGGGGTTGAAAGCCTCAACTTGAATCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTa                                                                                                       <  2:838362/150‑1 (MQ=255)
                                           aCGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTaa                                                                                                      <  2:122555/150‑1 (MQ=255)
                                                  gAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                                                               >  1:6213/1‑150 (MQ=255)
                                                   atacgTTACATCTACATCGCGATGTTCTTCTCTATTGCGGTTGATAGCCTAAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTTCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTcc                                                                                              <  1:167588/146‑1 (MQ=255)
                                                   aaaGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTcc                                                                                              <  2:321705/150‑1 (MQ=255)
                                                     aGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTg                                                                                            <  2:698518/150‑1 (MQ=255)
                                                      ggTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGc                                                                                           <  1:1051034/150‑1 (MQ=255)
                                                       gatACATCTACTTCGCGATCTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCCCAACAACAACACCCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCATTTACCCTCACAGATTAAGATTTTCCTGCt                                                                                          <  2:1635289/148‑1 (MQ=255)
                                                         tACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACACCTTTTACCCTCACAGTTTAAGATTTTCATGCttt                                                                                        >  1:126145/1‑150 (MQ=255)
                                                            aTCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa                                                                                     <  2:1158103/150‑1 (MQ=255)
                                                            aTCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCAAAGTTTAAGATTTTCCTGCTTTCaa                                                                                     >  1:1105557/1‑150 (MQ=255)
                                                             tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAg                                                                                    <  2:1003093/150‑1 (MQ=255)
                                                             tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAg                                                                                    <  1:1218610/150‑1 (MQ=255)
                                                             tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAg                                                                                    >  2:1136830/1‑150 (MQ=255)
                                                             tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAg                                                                                    <  2:1347742/150‑1 (MQ=255)
                                                             tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAg                                                                                    <  2:1573419/150‑1 (MQ=255)
                                                             tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAAATTTATTCGCAACAATAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAg                                                                                    <  2:1457592/150‑1 (MQ=255)
                                                              cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                                                                   <  2:121368/150‑1 (MQ=255)
                                                               tACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGat                                                                                  <  1:1365701/150‑1 (MQ=255)
                                                               tACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGat                                                                                  <  2:22366/150‑1 (MQ=255)
                                                                  ttCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatata                                                                               >  2:1367274/1‑150 (MQ=255)
                                                                           ttcttcTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCgg                                                                      <  2:1421547/150‑1 (MQ=255)
                                                                            tcttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGt                                                                     >  1:1368239/1‑150 (MQ=255)
                                                                             cttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATACGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTAAATCATTTTACCCTCACAGTTTAATATATTCATGCTTTCAAGATATATAACTTCGGtt                                                                    >  1:63079/1‑150 (MQ=255)
                                                                              ttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                                                                   <  2:1522390/150‑1 (MQ=255)
                                                                              gtctcTATTGCGGTTGAGAGCCTCAACTTGATTCGCAACAAAAAGACTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTAACAGTTTAAGATTTTCCTGATTTCAAGATATATAACGTCGGttt                                                                   >  1:908045/2‑150 (MQ=255)
                                                                                ctctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtat                                                                 <  2:1387787/150‑1 (MQ=255)
                                                                                 tctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtata                                                                >  1:1547252/1‑150 (MQ=255)
                                                                                 tctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtata                                                                <  2:707627/150‑1 (MQ=255)
                                                                                  ctATTGCGGTTTAAAGCCTCAACTTGAATCGCAACAAAACGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATaa                                                               <  2:1590451/150‑1 (MQ=255)
                                                                                     ttGCGGTTGAAAGCCTCAACTTGATTCTCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTAAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACa                                                            <  2:238874/150‑1 (MQ=255)
                                                                                     ttGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACa                                                            >  1:1006891/1‑150 (MQ=255)
                                                                                            tGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtatt                                                     <  2:1141285/150‑1 (MQ=255)
                                                                                             gAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtatta                                                    >  2:121280/1‑150 (MQ=255)
                                                                                                aGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGACGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTAATATCa                                                 >  2:663166/1‑150 (MQ=255)
                                                                                                 gCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTCCGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAt                                                >  1:1473435/1‑150 (MQ=255)
                                                                                                 gCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAt                                                >  1:506919/1‑150 (MQ=255)
                                                                                                  ccTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAta                                               >  1:471837/1‑150 (MQ=255)
                                                                                                  ccTCAACTTGATTCGCAACAAAAAGAAACCGCTCTGATACTCCGTACGCTCTCCGGCGGGAGGGCGTACTCACGCCTTTTACCCTCTCAGTTTAAGATTTTCCAGCATGCAAGCTATATAACGTCGGGCTATAAACAGACTATTATCAta                                               >  2:1426530/1‑150 (MQ=255)
                                                                                                   cTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtag                                              <  2:383616/150‑1 (MQ=255)
                                                                                                     cAACTTGATTCGCACCAAAAATAATACGCTCTGATACTCCGTACGCTCTCCGTCGTTAGGGCGTACTAACTCCTTATACCCTCACAGTATAAGATTTTCCTGCTTTAAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggt                                            <  1:131846/150‑1 (MQ=255)
                                                                                                      aaCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggta                                           <  2:485459/150‑1 (MQ=255)
                                                                                                       aCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtag                                          <  1:873065/150‑1 (MQ=255)
                                                                                                          tGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGcc                                       <  2:336685/150‑1 (MQ=255)
                                                                                                           gATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTTGGCCa                                      >  2:1224505/1‑150 (MQ=255)
                                                                                                           gATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCa                                      <  1:1338885/150‑1 (MQ=255)
                                                                                                                gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGa                                 >  2:660597/1‑150 (MQ=255)
                                                                                                                gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGGATAAGAGTTTCCTGCTTTCAAGATATATAACGTCGGGTTATAACCAGACTATTATCATAGGTAGGCCAAATGa                                 >  1:245907/1‑150 (MQ=255)
                                                                                                                 caacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAg                                <  1:1262545/150‑1 (MQ=255)
                                                                                                                  aacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAgg                               >  1:356914/1‑150 (MQ=255)
                                                                                                                  aacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAgg                               <  2:6213/150‑1 (MQ=255)
                                                                                                                     aaaaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATa                            >  2:954292/1‑150 (MQ=255)
                                                                                                                      aaaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTCCTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATaa                           >  2:1109934/1‑150 (MQ=255)
                                                                                                                        aaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAAcc                         <  1:1032803/150‑1 (MQ=255)
                                                                                                                         aGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCg                        <  1:549295/150‑1 (MQ=255)
                                                                                                                            aTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTATATAAACAGACTATTATCATAGGTAGGCCAATTGAGGATAACCGGAt                     >  1:24436/1‑150 (MQ=255)
                                                                                                                                gCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGaaa                 >  1:1074891/1‑150 (MQ=255)
                                                                                                                                   ctGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGaaaaaa              >  1:618325/1‑150 (MQ=255)
                                                                                                                                    tGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAAt             >  1:1669959/1‑150 (MQ=255)
                                                                                                                                     gATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATg            >  1:1326491/1‑150 (MQ=255)
                                                                                                                                      aTACTCCGTACGCTCTCCTGCGGGAGGTCGTAATCACTCCTTTTACCCTCACAGTTTAAGATTTTCATGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATgg           <  2:646523/150‑1 (MQ=255)
                                                                                                                                         gtgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATTGGTAGGGCAAATGAGGTTAACCGGATGAAAAAATGGGCa        >  1:950807/5‑150 (MQ=255)
                                                                                                                                         cTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCa        >  1:1494067/1‑150 (MQ=255)
                                                                                                                                         cTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCa        >  2:1019771/1‑150 (MQ=255)
                                                                                                                                           ccGTACGCGCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGACCAAATGAGGATAACCGGATGAAAAAATGGGCAGt      <  2:1023688/150‑1 (MQ=255)
                                                                                                                                             gTACGCCCTCCCGCAGGAGAGCGTACTCCCGCCTTTTACCCTCACAGTTTAAGATGTTCCTGCTGTCAAGATATATAACGTCGGTTTATACTCAGACTATTATCATAGGTAGGCCAAATGCGGATAACCGGATGAAAAAATGGGCAGgaa    >  1:1630678/1‑150 (MQ=255)
                                                                                                                                             gTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGtaa    >  1:705753/1‑150 (MQ=255)
                                                                                                                                               aCGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGtaata  >  1:1561296/1‑150 (MQ=255)
                                                                                                                                               aCGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGCGCAGtaata  >  1:845217/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATA  >  NC_000913/2970999‑2971291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: