Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,974,266 A→G 8.9% N583N (AAT→AAC)  aas ← fused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,974,2660AG 8.9% 283.0 / 14.7 101N583N (AAT→AAC) aasfused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase
Reads supporting (aligned to +/- strand):  ref base A (41/51);  new base G (4/5);  total (45/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 104 total aligned reads displayed.

GCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCC  >  NC_000913/2974117‑2974415
                                                                                                                                                     |                                                                                                                                                     
gCCAGTTGTTCCACCATTTCCAGCGCCACCATTTCTCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCTTCAATAAGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACa                                                                                                                                                       >  1:74718/1‑150 (MQ=255)
gCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACa                                                                                                                                                       >  1:1575345/1‑150 (MQ=255)
    gttgttCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATtct                                                                                                                                                   <  1:1538015/150‑1 (MQ=255)
          ccaccaTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTcggcgg                                                                                                                                             >  2:738189/1‑150 (MQ=255)
          ccaccaTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTcggcgg                                                                                                                                             <  1:1208886/150‑1 (MQ=255)
           caccaTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGt                                                                                                                                            >  1:596384/1‑150 (MQ=255)
             ctaTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGAGAATGTTCgcggcgaaat                                                                                                                                          <  1:1211485/148‑5 (MQ=255)
             ccaTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTgg                                                                                                                                          <  2:1709305/150‑1 (MQ=255)
             ccaTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGAGAATGTTCgcggcgaaat                                                                                                                                          <  1:495345/150‑5 (MQ=255)
             ccaTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGAGAATGTTCgcggcgaaat                                                                                                                                          <  1:5454/150‑5 (MQ=255)
             ccaTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGATCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGAGAATGTTCgcggcgaaat                                                                                                                                          <  1:193763/150‑5 (MQ=255)
              caTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTggg                                                                                                                                         <  1:1019854/150‑1 (MQ=255)
               aTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCACGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGc                                                                                                                                        >  1:282749/1‑150 (MQ=255)
                     aGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTcc                                                                                                                                  <  1:1477818/150‑1 (MQ=255)
                      gCGACACCAATTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCACTGCTCGTCAAAAAGCACAATATCGCCAGTGTCATACCAGCCTCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCa                                                                                                                                 <  2:894715/150‑1 (MQ=255)
                        cacaacaTTTCGCCTGCAAATTTTGCAAACCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCAGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGt                                                                                                                               <  1:1431389/149‑1 (MQ=255)
                          cacCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCCCAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCTCTTCCAGTac                                                                                                                             >  1:299269/1‑150 (MQ=255)
                            ccATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTacac                                                                                                                           <  1:1683952/150‑1 (MQ=255)
                                 tCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGc                                                                                                                      <  1:674411/150‑1 (MQ=255)
                                 tCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGc                                                                                                                      <  1:1065461/150‑1 (MQ=255)
                                   gCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCtt                                                                                                                    >  1:1556030/1‑150 (MQ=255)
                                    ccTGCAATTTTGGCAATGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATGGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTtc                                                                                                                   <  1:1074803/150‑1 (MQ=255)
                                     cTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTtct                                                                                                                  >  1:405293/1‑150 (MQ=255)
                                     cTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGACAGTGTCATACCACCCGCGCTCCATTTCGCCGCGAACATTCTCGCCGGTGGGCACTTCCAGTACACCTGGCTtct                                                                                                                  >  2:1411223/1‑150 (MQ=255)
                                     atGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGCATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACCCCTGGCTtcg                                                                                                                  >  1:279061/2‑149 (MQ=255)
                                       gCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATATCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTcc                                                                                                                <  1:1141433/150‑1 (MQ=255)
                                             ttGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGc                                                                                                          <  2:1235792/150‑1 (MQ=255)
                                               ggCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCCCCCGCAg                                                                                                        >  1:1567977/1‑150 (MQ=255)
                                               ggCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAg                                                                                                        >  2:954281/1‑150 (MQ=255)
                                                gCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCGGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGACCATTCTCGGCGGTGGGCACTTCCAGTTCACCTGGCTTCTCCACCCGCTGa                                                                                                       >  1:1186912/1‑150 (MQ=255)
                                                      cGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCg                                                                                                 <  2:248663/150‑1 (MQ=255)
                                                        ttttGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCTCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGtt                                                                                               <  1:840344/150‑1 (MQ=255)
                                                         tttGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTc                                                                                              <  2:1411898/150‑1 (MQ=255)
                                                         tttGCGCGGCCCGGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTc                                                                                              <  2:986766/150‑1 (MQ=255)
                                                             cgcgGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTTATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTa                                                                                          <  2:1031657/150‑1 (MQ=255)
                                                                   ccTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCCCCCGCAGATAGCCGTTCATTATGTTCg                                                                                    >  2:1191830/1‑150 (MQ=255)
                                                                      taaTCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGACCATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGcc                                                                                 <  1:841426/149‑1 (MQ=255)
                                                                       aaTCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGccc                                                                                <  2:546733/150‑1 (MQ=255)
                                                                       aaTCTGCACAAAGCCCTGCGCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGccc                                                                                <  2:62428/150‑1 (MQ=255)
                                                                           tGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGTCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTc                                                                            <  1:69101/150‑1 (MQ=255)
                                                                           tGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGCTAGCCGTTCATTATGTTCGGCCCTTTc                                                                            >  2:477712/1‑150 (MQ=255)
                                                                           tGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTc                                                                            <  2:1121470/150‑1 (MQ=255)
                                                                           tGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGGTCATTATGTTCGGCCCTTTc                                                                            <  2:279061/150‑1 (MQ=255)
                                                                            gCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCa                                                                           <  1:1034444/150‑1 (MQ=255)
                                                                              acaAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGt                                                                         <  1:362736/150‑1 (MQ=255)
                                                                              acaAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGt                                                                         <  2:1100861/150‑1 (MQ=255)
                                                                              acaAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTAACCCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGt                                                                         >  1:346492/1‑150 (MQ=255)
                                                                                acaGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGGCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTg                                                                       <  1:1342127/148‑1 (MQ=255)
                                                                                   gCCTTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCCGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAg                                                                    <  2:168544/150‑1 (MQ=255)
                                                                                    cccTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAgg                                                                   <  1:849453/150‑1 (MQ=255)
                                                                                    cccTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAgg                                                                   <  2:665336/150‑1 (MQ=255)
                                                                                     ccTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCCCCCGCAGATAGCCGTTCATTATGTTCGGCCCTGTCAGTTGCCGGc                                                                  >  2:511901/1‑150 (MQ=255)
                                                                                     ccTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGc                                                                  <  1:1630472/150‑1 (MQ=255)
                                                                                         cTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTcc                                                              >  2:1431822/1‑150 (MQ=255)
                                                                                         cTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTcc                                                              >  1:533657/1‑150 (MQ=255)
                                                                                           cGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTGCCAGTAAACCTGGCGTCTCCACCCGCCGATAGCCGGTCATTATGTTCGGCCCGTTCAGTGGCCGGCGTCCGc                                                            >  1:1089344/1‑150 (MQ=255)
                                                                                           cGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCGCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTGCTCCACCCGCAGAGAGCCGTTCATTATGTTCGGCCCTTTCAGGTGCAGGCGTCCGc                                                            <  1:1046573/150‑1 (MQ=255)
                                                                                             tCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCGCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGccc                                                          <  2:227684/150‑1 (MQ=255)
                                                                                              cAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCct                                                         <  2:610642/150‑1 (MQ=255)
                                                                                                 aaCGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGGCCGCCCTCtt                                                      <  1:954281/150‑1 (MQ=255)
                                                                                                  aCGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTc                                                     <  2:1575345/150‑1 (MQ=255)
                                                                                                  aCGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTc                                                     <  2:1493182/150‑1 (MQ=255)
                                                                                                   cGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCg                                                    >  1:1212455/1‑150 (MQ=255)
                                                                                                   cGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCg                                                    >  1:748345/1‑150 (MQ=255)
                                                                                                    gCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGa                                                   >  1:773994/1‑150 (MQ=255)
                                                                                                         atatCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCa                                              <  1:1199179/150‑1 (MQ=255)
                                                                                                         atatCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCa                                              <  2:1701881/150‑1 (MQ=255)
                                                                                                         atatCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCGTCGATACCa                                              >  2:394796/1‑150 (MQ=255)
                                                                                                          tatCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAg                                             <  2:1613476/150‑1 (MQ=255)
                                                                                                          tatCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAg                                             <  2:4780/150‑1 (MQ=255)
                                                                                                             cGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGa                                          >  2:179053/1‑150 (MQ=255)
                                                                                                              gCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGCTAGCAGGGAc                                         >  1:733673/1‑150 (MQ=255)
                                                                                                                 aGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGAGCACTTCCAGTACCCCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGa                                      >  2:915227/1‑150 (MQ=255)
                                                                                                                    gtCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGacaa                                   >  1:1380729/1‑150 (MQ=255)
                                                                                                                      cATACCAGCCGCGCTCCATTTCGCCGCGGACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCGTCGCTACCAGGGACCGacacca                                 >  1:1414012/1‑150 (MQ=255)
                                                                                                                       aTACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAg                                <  1:1274625/150‑1 (MQ=255)
                                                                                                                        tACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAgg                               >  1:492212/1‑150 (MQ=255)
                                                                                                                                gcgcTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATc                       <  2:1543868/150‑1 (MQ=255)
                                                                                                                                gcgcTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCATCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATc                       >  2:112800/1‑150 (MQ=255)
                                                                                                                                      cccaccgccgaGAATGTTCGCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGTCCGATAACAGGCGCGCATCCATTcc                 >  2:863648/6‑150 (MQ=255)
                                                                                                                                      cccaccgccgaGAATGTTCGCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTcc                 >  1:547670/6‑150 (MQ=255)
                                                                                                                                      cATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTcc                 <  1:1548157/150‑1 (MQ=255)
                                                                                                                                       aTTTCGCCGCGAACATTCTCGGCGTTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGAAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCt                <  2:1086204/150‑1 (MQ=255)
                                                                                                                                        tttCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCGCCCGCAGCTAGCCGTTCAATATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGAGCGACACCAGGCGCGCATCCATTccat               >  2:230250/1‑148 (MQ=255)
                                                                                                                                        tttCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTg               <  2:61699/150‑1 (MQ=255)
                                                                                                                                        tttAGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGTTAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTg               <  2:949080/150‑1 (MQ=255)
                                                                                                                                        caccgccgaGAATGTTCGCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTg               >  2:79308/4‑150 (MQ=255)
                                                                                                                                         accgccgaGAATGTTCGCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTgg              <  2:547670/148‑1 (MQ=255)
                                                                                                                                          tCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGt             <  1:988989/150‑1 (MQ=255)
                                                                                                                                          ccgccgaGAATGTTCGCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCCGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGt             >  1:1324751/2‑150 (MQ=255)
                                                                                                                                            gccgcGAACATTCTCGGCGGTGGGCACTTCCAGTACCCCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGCCCGACAGCAGGCGCGCATCCATTCCTGGTAg           >  1:953406/1‑150 (MQ=255)
                                                                                                                                            gccgcGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCGTTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACCACAGGCGCGCATCCATTCCTGGTAg           >  2:733866/1‑150 (MQ=255)
                                                                                                                                            gccgcGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAg           >  1:604747/1‑150 (MQ=255)
                                                                                                                                            cccgcGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTATCAGTTGCAGGCGTCCCCCCTCTTCGAACCCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAg           >  1:237848/2‑150 (MQ=255)
                                                                                                                                             ccgcGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGa          <  2:783148/150‑1 (MQ=255)
                                                                                                                                               gcgAACAGTCTCGGCGGTGGGCACATCCAGTACACCTGGCGTCTCCACCCGCAGATAGCCGTTCAATATGTTCGGCCCTTGCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAAt        <  1:255731/150‑1 (MQ=255)
                                                                                                                                                 gAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATAc      >  1:1328368/1‑150 (MQ=255)
                                                                                                                                                 gAACATTCTCGGCGGTGGGCACTTCCAGTACAACTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTATCAGTTGCCGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATAc      >  1:477292/1‑150 (MQ=255)
                                                                                                                                                   aCATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCATCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAAGACGc    >  2:1602394/1‑150 (MQ=255)
                                                                                                                                                     aTTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGccc  <  2:1212455/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCC  >  NC_000913/2974117‑2974415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑