Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,976,321 C→A 7.6% noncoding (65/82 nt) omrB ← sRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,976,3210CA 7.6% 315.4 / 7.5 114noncoding (65/82 nt)omrBsRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base C (49/55);  new base A (3/7);  total (52/62)
Fisher's exact test for biased strand distribution p-value = 3.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

CCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGC  >  NC_000913/2976172‑2976467
                                                                                                                                                      |                                                                                                                                                  
ccAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATTACCGGCAAAAAAAACCTGCGCATc                                                                                                                                                    >  2:667565/1‑150 (MQ=255)
   atcaatACCTCTGGGATCTTGATTGTGGTCTGCACGACGCCTCTTCGCCAGCGAGAAAACGCAAAGCAATGAAGGGAAATGCAACGAGGTGTGTATATTGTCGGATACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTgc                                                                                                                                                 >  2:1059789/1‑150 (MQ=255)
    tcaatACCTCTGGGATATTGATTGAGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGATACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTgcg                                                                                                                                                <  2:1152066/150‑1 (MQ=255)
     caatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAATGAATGAAGGTAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTgcgc                                                                                                                                               <  2:1053835/150‑1 (MQ=255)
     caatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATgcgc                                                                                                                                               <  2:1682828/150‑1 (MQ=255)
        tcccTCTGGGATCTGGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAgg                                                                                                                                            <  1:255438/148‑1 (MQ=255)
            tctGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATGCGCAggtttt                                                                                                                                        <  1:1031334/150‑5 (MQ=255)
              tGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGgtg                                                                                                                                      <  2:406526/150‑1 (MQ=255)
               gggATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGgtgt                                                                                                                                     >  1:1478512/1‑150 (MQ=255)
               gggATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATGCGCAggttttttt                                                                                                                                     <  1:958827/150‑8 (MQ=255)
               gggATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATGCGCAggttttttt                                                                                                                                     <  2:307067/150‑8 (MQ=255)
                 gATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGAACGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTaa                                                                                                                                   >  2:707346/1‑150 (MQ=255)
                      tGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAt                                                                                                                              <  1:435735/150‑1 (MQ=255)
                      tGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAt                                                                                                                              >  1:278874/1‑150 (MQ=255)
                           gtgGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCt                                                                                                                         >  2:242013/1‑150 (MQ=255)
                                cTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAcc                                                                                                                    <  2:13202/150‑1 (MQ=255)
                                cTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAcc                                                                                                                    >  1:529332/1‑150 (MQ=255)
                                cTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAcc                                                                                                                    <  1:700427/150‑1 (MQ=255)
                                  gCACTACGACTCTTCGCCAGCGAGAAAACTCAAAGGAATGAATGGAAATGCAACGAGGTGTGTAAATTGTCGGTTAGTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCATCCCg                                                                                                                  <  2:509303/150‑1 (MQ=255)
                                  gCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGACTGTAAATTGTCGGTTACTGTTACACATTGATGACCGGCAAAAAAAACCTGCGCATATGCGCAGGCTGGTCTAATTCATGTGCTCAAcccc                                                                                                                  >  1:473051/1‑149 (MQ=255)
                                    acgacgACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGaa                                                                                                                >  2:1228027/1‑150 (MQ=255)
                                     cgacgaCTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAg                                                                                                               <  1:1709825/150‑1 (MQ=255)
                                       acgacTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGAACGGCAAAAAAAACCTGCGCAACTGCGCAGGCTGATTTAATTCAATTGCTAAACACCAAGtt                                                                                                             >  1:511732/1‑150 (MQ=255)
                                        cgacTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGAGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTg                                                                                                            >  1:1273292/1‑150 (MQ=255)
                                          acTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGAc                                                                                                          <  1:534324/150‑1 (MQ=255)
                                          acTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGAc                                                                                                          <  1:972801/150‑1 (MQ=255)
                                           ctctTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGAGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACt                                                                                                         >  1:529751/1‑150 (MQ=255)
                                               tCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCAc                                                                                                     <  1:1053401/150‑1 (MQ=255)
                                               tCGCAAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCATCGAGGTGTGTAAATTGTCGGTTACTGTTACATATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCAc                                                                                                     <  1:102854/150‑1 (MQ=255)
                                                cGCCAGCGAGAAAACGCAACGGAATTACGGGAAATGCAACCAGGTGTGAAAATTGGCGGTTACTGGTACAGATTGATGACCGGCAAAAAAAAACTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCAcc                                                                                                    <  2:46391/150‑1 (MQ=255)
                                                cGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGCCTTCAcc                                                                                                    >  2:60392/1‑150 (MQ=255)
                                                cGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGGCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCAcc                                                                                                    <  2:878058/150‑1 (MQ=255)
                                                 gCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCt                                                                                                   >  2:235464/1‑150 (MQ=255)
                                                 gCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCt                                                                                                   >  2:1452507/1‑150 (MQ=255)
                                                  ccAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTa                                                                                                  >  2:138417/1‑150 (MQ=255)
                                                    aGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATc                                                                                                <  2:1448860/150‑1 (MQ=255)
                                                    aGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATc                                                                                                <  2:341894/150‑1 (MQ=255)
                                                    aGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATc                                                                                                <  2:354821/150‑1 (MQ=255)
                                                     gCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCa                                                                                               <  2:1478512/150‑1 (MQ=255)
                                                      cGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCaa                                                                                              >  2:635651/1‑150 (MQ=255)
                                                        agaAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATa                                                                                            >  2:176520/1‑150 (MQ=255)
                                                          aaaaCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATAcc                                                                                          <  2:150197/150‑1 (MQ=255)
                                                          aaaaCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATAcc                                                                                          <  2:516314/150‑1 (MQ=255)
                                                               gCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTgg                                                                                     <  2:1124281/150‑1 (MQ=255)
                                                               gCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTgg                                                                                     <  1:958183/150‑1 (MQ=255)
                                                                cAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTggg                                                                                    <  1:176520/150‑1 (MQ=255)
                                                                cAAAGGAAGGAAGGGAAATGCAAAGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCTGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTTCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTggg                                                                                    <  2:507095/150‑1 (MQ=255)
                                                                 aaaGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGa                                                                                   >  1:1489710/1‑150 (MQ=255)
                                                                   aGGAATGAAGGGATATGCAACGAGGTGTGTAAAATGTCTGTTACTGTTACAGAGTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATc                                                                                 <  1:869826/150‑1 (MQ=255)
                                                                   aGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATc                                                                                 <  1:1100226/150‑1 (MQ=255)
                                                                    ggAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATca                                                                                >  1:141516/1‑150 (MQ=255)
                                                                         gAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACt                                                                           >  2:1121582/1‑150 (MQ=255)
                                                                          aaGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACt                                                                           >  2:912077/1‑150 (MQ=255)
                                                                             ggAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGAGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAg                                                                       <  2:1575658/150‑1 (MQ=255)
                                                                              gAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGc                                                                      >  1:386045/1‑150 (MQ=255)
                                                                                   gCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCt                                                                 >  1:558299/1‑150 (MQ=255)
                                                                                   gCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCt                                                                 >  1:728977/1‑150 (MQ=255)
                                                                                    cAACGAGTTGTGTAAATTTTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCGCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTg                                                                <  1:1523339/150‑1 (MQ=255)
                                                                                    cAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATATGCGCAGGCTTGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTg                                                                <  2:1688406/150‑1 (MQ=255)
                                                                                       cGAGGTGTGTAAATTGTCTGTTACTGTTACAGATTGATGACAGGCAAAAAAAACCTGCGCATCTGCTCAGGCTTGTGTAATTCATGTGATCTACCCGAATTTTACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAg                                                             <  1:460658/150‑1 (MQ=255)
                                                                                       cGAGGTGTGTAAATTGTCGGTTTCTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAg                                                             >  2:1521693/1‑150 (MQ=255)
                                                                                       cGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAg                                                             >  1:476990/1‑150 (MQ=255)
                                                                                       cGAGGTGTGTAAAATGTCTGTTACTGTTACAGATTGAAGACATTAAAAAATATCCTGCGCTTATGCGCAGGCTGGTGTCATTCATGTGCGCAACCCGATTTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAg                                                             <  2:1237798/150‑1 (MQ=255)
                                                                                        gAGGTGTGTAAATTGTCGGTTTCTTTTACATATTTATGAGAGGCAAAATAAACCTTCGGATCTGCTCAGTTTGGTTTAATTCATGTGTTCATATCTATTTTTATTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGc                                                            <  2:675058/150‑1 (MQ=255)
                                                                                          ggTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCa                                                          >  1:817942/1‑150 (MQ=255)
                                                                                             gtgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAAc                                                       >  1:1461398/1‑150 (MQ=255)
                                                                                              tgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACg                                                      <  2:1489710/150‑1 (MQ=255)
                                                                                                 aaaTTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTAATGTGCTCAACCCGAAGTTGACTTCACATATCATAACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGccc                                                   >  2:1102850/1‑149 (MQ=255)
                                                                                                      gTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGc                                              <  1:819431/150‑1 (MQ=255)
                                                                                                        cGGTTACTGTTACAGATTGACGACCGGCAAAAAAAACCTGCGCATCTGAGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCAATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCgg                                            <  1:942551/150‑1 (MQ=255)
                                                                                                         ggTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGTCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATAACTCTGGGATCACAACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGt                                           >  2:495529/1‑150 (MQ=255)
                                                                                                         ggTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGt                                           <  2:1354189/150‑1 (MQ=255)
                                                                                                          gTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTc                                          <  1:667565/150‑1 (MQ=255)
                                                                                                          gTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTc                                          <  1:1189823/150‑1 (MQ=255)
                                                                                                                gTTACAGATTGATGACCGGCAAAAAAAAACAGCGCATCTGCGCAGGGTGGTGTAAATCATGTGCTCAACCCGAAGTTGACATCACCTATCAATACCTCAGGGAACACCAAATTAGCAACCTGAAGCCAAACGCCAACCGCGGTAAATAGc                                    >  1:194422/1‑150 (MQ=255)
                                                                                                                gTTACAGATTGATGACAGTAAAAAAAATCATGCGCAGATGCGCAGGCTGGTGTAATTCATCTGCTCTACCAGATGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGc                                    <  2:196912/150‑1 (MQ=255)
                                                                                                                   aaaGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATCTGCTCAACCCGATGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAAc                                 <  1:837531/148‑1 (MQ=255)
                                                                                                                    cAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACa                                <  1:606467/150‑1 (MQ=255)
                                                                                                                    cAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACa                                >  2:216656/1‑150 (MQ=255)
                                                                                                                     aGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAg                               >  1:997495/1‑150 (MQ=255)
                                                                                                                     aGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAg                               >  2:1716561/1‑150 (MQ=255)
                                                                                                                     aGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTAAATCGCAACac                               >  2:125626/1‑149 (MQ=255)
                                                                                                                       aTTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCg                             >  1:1023563/1‑150 (MQ=255)
                                                                                                                          gatgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGttt                          >  1:1314458/1‑150 (MQ=255)
                                                                                                                           agtaCCGCCAAAAAAAACCTTCGCATCTGCTCAGGCAGGTGAAATTCATCTCCTCAACCAGAACTTTACTTCACCAATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTc                         <  1:1002874/147‑1 (MQ=255)
                                                                                                                            tgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCg                        <  1:1121582/150‑1 (MQ=255)
                                                                                                                            tgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCg                        <  1:1228027/150‑1 (MQ=255)
                                                                                                                                 ggCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCCTGTGCTCAACCCGAAGTCGACATCACCACTCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAg                   <  1:134876/150‑1 (MQ=255)
                                                                                                                                 ggCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAg                   <  1:242013/150‑1 (MQ=255)
                                                                                                                                 ggCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAg                   <  2:1023563/150‑1 (MQ=255)
                                                                                                                                   cAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAgtg                 >  2:168768/1‑150 (MQ=255)
                                                                                                                                      aaaaaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCCCCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTa               >  1:1480433/1‑150 (MQ=255)
                                                                                                                                      aaaaaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTa               >  1:1487127/1‑150 (MQ=255)
                                                                                                                                        accagccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCCGCGGTCAATCGCAACAGCGTTTCGAAAAGAGTAAc             >  2:131857/6‑150 (MQ=255)
                                                                                                                                         ccagccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGCAGCCAACCGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAAcc            >  1:1327615/5‑150 (MQ=255)
                                                                                                                                               tGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGt      >  2:56470/1‑150 (MQ=255)
                                                                                                                                                 cgcATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGttt    <  1:1521693/150‑1 (MQ=255)
                                                                                                                                                  gcATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTg   >  1:1282989/1‑150 (MQ=255)
                                                                                                                                                   cATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGc  <  1:707346/150‑1 (MQ=255)
                                                                                                                                                   cATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGc  <  1:338993/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
CCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGC  >  NC_000913/2976172‑2976467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: