Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,987,103 C→T 7.6% intergenic (+27/‑433) yqeG → / → yqeH putative transporter/putative LuxR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,987,1030CT 7.6% 431.6 / 16.6 144intergenic (+27/‑433)yqeG/yqeHputative transporter/putative LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (58/74);  new base T (5/6);  total (63/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.16e-01

Only 100 of 149 total aligned reads displayed.

CTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACTT  >  NC_000913/2986954‑2987252
                                                                                                                                                     |                                                                                                                                                     
cTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGc                                                                                                                                                       >  1:65400/1‑150 (MQ=255)
cTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGc                                                                                                                                                       >  1:581846/1‑150 (MQ=255)
 ttttCATCATGCCTACGCTGTCAACGTATCTCATCACGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCt                                                                                                                                                      >  2:152644/1‑150 (MQ=255)
       tcatGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAtt                                                                                                                                                >  1:850923/1‑150 (MQ=255)
       tcatGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAtt                                                                                                                                                >  1:598600/1‑150 (MQ=255)
       tcatGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAtt                                                                                                                                                >  1:228346/1‑150 (MQ=255)
       tcatGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGATAATATCAGTTGTTGATGGTTCAAAATTGCTACAAtt                                                                                                                                                >  2:1184601/1‑150 (MQ=255)
        catGCCTACGCTGTCAACGTATCTCATCCCGTCGCTAAAACCCTGGCGTTCCATCGGAAATCTGATTACGATGCTCGTGGGTATCCTGTGAGTATCGGAAAAGTACATTAGATAATATCAGATGTTGATGTTTAAAAATTGCTACAAatt                                                                                                                                               >  2:864745/1‑150 (MQ=255)
          tGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGGTCAAAATTGCTACAATTTTg                                                                                                                                             <  1:507415/150‑1 (MQ=255)
           gCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGTAGCAATTTTGa                                                                                                                                            <  1:693009/150‑1 (MQ=255)
            ccTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGat                                                                                                                                           >  2:1623394/1‑149 (MQ=255)
             cTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGTAGCAATTTTGAAc                                                                                                                                          <  1:451198/150‑1 (MQ=255)
             cTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAc                                                                                                                                          <  1:1443337/150‑1 (MQ=255)
              tACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGTAGCAATTTTGAAcc                                                                                                                                         <  1:635320/150‑1 (MQ=255)
              tACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAcc                                                                                                                                         <  1:1011058/150‑1 (MQ=255)
              tACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAcc                                                                                                                                         <  1:711464/150‑1 (MQ=255)
              tACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAcc                                                                                                                                         >  2:729892/1‑150 (MQ=255)
                     tCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccattccat                                                                                                                                  <  1:1002596/150‑1 (MQ=255)
                      cAACGTATCTCATCCCGGTGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccattccatt                                                                                                                                 >  2:431617/1‑150 (MQ=255)
                        aCGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTACAtttt                                                                                                                               >  1:1416596/1‑150 (MQ=255)
                          gTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTc                                                                                                                             <  2:1592835/150‑1 (MQ=255)
                             tctcATCCCGGCGCTTAAACCCTGGCGATCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGGTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCata                                                                                                                          <  1:228503/150‑1 (MQ=255)
                              ctcATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataa                                                                                                                         >  2:795862/1‑150 (MQ=255)
                               tcatcaCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataat                                                                                                                        <  1:709735/150‑1 (MQ=255)
                               tcATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTAACCATTCCATTTTTCataata                                                                                                                       >  2:1380468/1‑150 (MQ=255)
                                cATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataata                                                                                                                       >  2:563855/1‑150 (MQ=255)
                                   cccGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATAtta                                                                                                                    >  1:84255/1‑149 (MQ=255)
                                      ggCGCTTAAACCCTGGCGTTCGATCGGAAATCTGATTACGCTTATCGTGGGTATCCTGTGCGTTTCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTGATAATATTGAtt                                                                                                                 <  2:1484002/150‑1 (MQ=255)
                                       gcgcTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCAttt                                                                                                                <  2:113244/150‑1 (MQ=255)
                                          cTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCATTAGCTAATATCAGTTGTGGATGGATCAAAATTGCAACAATTTTGAACCATTACATTTTTCATAATATTAATTTGtt                                                                                                             >  1:399547/1‑150 (MQ=255)
                                             aaaCCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTCGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAGTATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTATTCATAATATTAATTTGttatt                                                                                                          >  2:1435263/1‑150 (MQ=255)
                                                cccTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTattatt                                                                                                       >  2:876115/1‑150 (MQ=255)
                                                 ccTTGCGTTCCATCTGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTc                                                                                                      <  1:714887/150‑1 (MQ=255)
                                                 ccTGGCGTTCCATCGGAAATATGATTACGCTGATCGTTGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTc                                                                                                      <  1:697362/150‑1 (MQ=255)
                                                  cTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCATGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTcc                                                                                                     >  1:709956/1‑150 (MQ=255)
                                                   tGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCa                                                                                                    >  2:1702540/1‑150 (MQ=255)
                                                    ggCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCAc                                                                                                   >  1:426869/1‑150 (MQ=255)
                                                      cGTTCCATCGTAAATCTGATTACGCTGATCTTGGGTATCCTGTGCGTATCTGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCAccc                                                                                                 <  1:276138/150‑1 (MQ=255)
                                                            aTCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTACTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCAAACCTATCAc                                                                                           >  2:449883/1‑150 (MQ=255)
                                                             tCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACt                                                                                          <  1:1342917/150‑1 (MQ=255)
                                                             tCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAAAGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATAACt                                                                                          >  1:114218/1‑150 (MQ=255)
                                                              cGGAAATCTTATTACGCTGATCTTGGGTATCCTTTTCGTATCGGTAATGTTCTTTAGCTAATATCAGTTTTGGATGGTTCAAAATTGCTACAATTTTGAACGATTACATTTTTCATAATATTCATTTGTTATTATTCAACCCTATCACtt                                                                                         >  2:17682/1‑150 (MQ=255)
                                                              cGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACtt                                                                                         <  1:1542928/150‑1 (MQ=255)
                                                               ggAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGAATAGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAATTTGAACCATTCCATTTTTAATAATATAAATTTTTAATAATAACACCATATCACTtc                                                                                        >  1:381313/1‑150 (MQ=255)
                                                                 aaaTCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtctc                                                                                      >  2:621543/1‑150 (MQ=255)
                                                                  aaTCTGATTACGCTGATCGTGGGTATCCTGTGCGTATAGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtcgct                                                                                     <  2:1159524/150‑1 (MQ=255)
                                                                    tCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCg                                                                                   <  2:1443740/150‑1 (MQ=255)
                                                                    tCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCg                                                                                   <  1:1251334/150‑1 (MQ=255)
                                                                     cTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGt                                                                                  <  1:436820/150‑1 (MQ=255)
                                                                     cTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGt                                                                                  <  2:1573593/150‑1 (MQ=255)
                                                                     cTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGt                                                                                  <  1:802528/150‑1 (MQ=255)
                                                                     cTGATTACGCTGAACGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGt                                                                                  <  1:1150055/150‑1 (MQ=255)
                                                                         ttACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCata                                                                              <  2:1054950/150‑1 (MQ=255)
                                                                         ttACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCata                                                                              <  2:850923/150‑1 (MQ=255)
                                                                          tACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCatat                                                                             >  1:445407/1‑150 (MQ=255)
                                                                               tGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAg                                                                        <  2:1024591/150‑1 (MQ=255)
                                                                                      gggTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGtattata                                                                 >  1:1660516/1‑149 (MQ=255)
                                                                                      gggTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATc                                                                 <  2:223487/150‑1 (MQ=255)
                                                                                          aTCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagtt                                                             <  2:797658/150‑1 (MQ=255)
                                                                                             cTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATAACCTAGTATAATCagttagt                                                          >  2:900759/1‑150 (MQ=255)
                                                                                               gtgCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAgttagtta                                                        <  1:1333965/150‑1 (MQ=255)
                                                                                                 gCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTaaa                                                      <  1:866062/150‑1 (MQ=255)
                                                                                                     aTCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAacac                                                   <  2:635829/149‑1 (MQ=255)
                                                                                                       cGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAAcacaca                                                <  1:64534/150‑1 (MQ=255)
                                                                                                        ggTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCATAGTATTATCAGTTAGTTAAAAACACaa                                               >  1:773182/1‑150 (MQ=255)
                                                                                                               ttCTTTAGCTAATATCAGTTGTGTATGTTTCAAAATTGATACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATc                                        <  1:1467400/150‑1 (MQ=255)
                                                                                                               ttCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATc                                        <  2:1598355/150‑1 (MQ=255)
                                                                                                               ttCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATc                                        <  2:46879/150‑1 (MQ=255)
                                                                                                                tCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCt                                       <  2:65400/150‑1 (MQ=255)
                                                                                                                tCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTAATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCt                                       <  2:130130/150‑1 (MQ=255)
                                                                                                                 cTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTg                                      <  2:1637368/150‑1 (MQ=255)
                                                                                                                 cTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTg                                      >  2:243243/1‑150 (MQ=255)
                                                                                                                    tAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTTGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAAc                                   <  1:1702540/150‑1 (MQ=255)
                                                                                                                       cTAATATCAGTTGTTGTTGGTTAAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATc                                <  1:1529847/150‑1 (MQ=255)
                                                                                                                          atatCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTa                             <  2:1531100/150‑1 (MQ=255)
                                                                                                                          atatCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTa                             <  2:581846/150‑1 (MQ=255)
                                                                                                                            atCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTaaa                           >  2:53668/1‑150 (MQ=255)
                                                                                                                            atCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTaaa                           <  2:1646561/150‑1 (MQ=255)
                                                                                                                             tCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAAt                          >  2:1466481/1‑150 (MQ=255)
                                                                                                                               aGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCAAGTATTATCAGTTAGTTAAACACACAAAGATATCTGAACAACTTAAATaa                        >  2:592320/1‑150 (MQ=255)
                                                                                                                                gTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATaac                       >  2:1385056/1‑149 (MQ=255)
                                                                                                                                  tgtgTATTGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCAACCTATCAATTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGAc                     <  1:1184601/150‑1 (MQ=255)
                                                                                                                                  tgtgGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGAc                     <  2:699744/150‑1 (MQ=255)
                                                                                                                                      gATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTTGTTAAACACACAAAGAGATCTGAACATCTTATATAAGACTAtt                 >  2:1126167/1‑150 (MQ=255)
                                                                                                                                      gATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTAtt                 <  2:621315/150‑1 (MQ=255)
                                                                                                                                        tGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTa               <  1:1412295/150‑1 (MQ=255)
                                                                                                                                          gTTCAAAATTGTAGCAATTTTTAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCATATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAATAGATCTGAACATATTAAATAATACTATTTAAg             >  2:1571284/1‑150 (MQ=255)
                                                                                                                                          gTTCAAAATTGTAGCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAg             >  1:907406/1‑150 (MQ=255)
                                                                                                                                          gTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAg             >  1:1115693/1‑150 (MQ=255)
                                                                                                                                          gTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTAGTCATATCCTAGTTTTATCATTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAATACTATTTAAg             >  1:99513/1‑150 (MQ=255)
                                                                                                                                           ttCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAGTCTTATTTAAGa            <  2:576377/150‑1 (MQ=255)
                                                                                                                                            tCAAAATTGCTACAATTTTGCACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGAt           >  1:908946/1‑150 (MQ=255)
                                                                                                                                            tCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAGTCTTATTTAAGAt           <  1:876115/150‑1 (MQ=255)
                                                                                                                                            tCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGAt           <  1:621543/150‑1 (MQ=255)
                                                                                                                                              aaaaTTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGc         <  1:1606769/150‑1 (MQ=255)
                                                                                                                                                aaTTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAGTCTTATTTAAGATgttc       <  1:179025/150‑4 (MQ=255)
                                                                                                                                                  ttGCTACAATTTTGAACCATTCCATTTTTCATAATATTAATTTGTTATTATTCCACCCTATCTCTTCTCTCGTCATATACTAGTATTATCAGTTAGTTAAACACAAAAAGAGATCTGAACATCTTAAATAGTCTTATTTAAGATgttcag     <  2:1241793/150‑6 (MQ=255)
                                                                                                                                                   tGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAAc    <  1:504334/150‑1 (MQ=255)
                                                                                                                                                     cTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACtt  >  2:2432/1‑150 (MQ=255)
                                                                                                                                                     cTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTAGTCATATCCTAGTATTATCAGTTATTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGAATAACtt  >  1:288620/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACTT  >  NC_000913/2986954‑2987252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: