Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,987,104 T→A 8.0% intergenic (+28/‑432) yqeG → / → yqeH putative transporter/putative LuxR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,987,1040TA 8.0% 396.5 / 19.0 145intergenic (+28/‑432)yqeG/yqeHputative transporter/putative LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (58/75);  new base A (6/6);  total (64/81)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.60e-01

Only 100 of 148 total aligned reads displayed.

TTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACTTA  >  NC_000913/2986955‑2987253
                                                                                                                                                     |                                                                                                                                                     
ttttCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCt                                                                                                                                                       >  1:46879/1‑150 (MQ=255)
ttttCATCATGCCTACGCTGTCAACGTATCTCATCACGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCt                                                                                                                                                       >  2:152644/1‑150 (MQ=255)
      tcatGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAtt                                                                                                                                                 >  1:850923/1‑150 (MQ=255)
      tcatGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAtt                                                                                                                                                 >  1:228346/1‑150 (MQ=255)
      tcatGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGATAATATCAGTTGTTGATGGTTCAAAATTGCTACAAtt                                                                                                                                                 >  2:1184601/1‑150 (MQ=255)
       catGCCTACGCTGTCAACGTATCTCATCCCGTCGCTAAAACCCTGGCGTTCCATCGGAAATCTGATTACGATGCTCGTGGGTATCCTGTGAGTATCGGAAAAGTACATTAGATAATATCAGATGTTGATGTTTAAAAATTGCTACAAatt                                                                                                                                                >  2:864745/1‑150 (MQ=255)
         tGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGGTCAAAATTGCTACAATTTTg                                                                                                                                              <  1:507415/150‑1 (MQ=255)
          gCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGTAGCAATTTTGa                                                                                                                                             <  1:693009/150‑1 (MQ=255)
           ccTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGat                                                                                                                                            >  2:1623394/1‑149 (MQ=255)
            cTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGTAGCAATTTTGAAc                                                                                                                                           <  1:451198/150‑1 (MQ=255)
            cTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAc                                                                                                                                           <  1:1443337/150‑1 (MQ=255)
             tACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAcc                                                                                                                                          <  1:1011058/150‑1 (MQ=255)
             tACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAcc                                                                                                                                          <  1:711464/150‑1 (MQ=255)
             tACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAcc                                                                                                                                          >  2:729892/1‑150 (MQ=255)
                    tCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccattccat                                                                                                                                   <  1:1002596/150‑1 (MQ=255)
                     cAACGTATCTCATCCCGGTGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccattccatt                                                                                                                                  >  2:431617/1‑150 (MQ=255)
                       aCGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTACAtttt                                                                                                                                >  1:1416596/1‑150 (MQ=255)
                         gTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTc                                                                                                                              <  2:1592835/150‑1 (MQ=255)
                            tctcATCCCGGCGCTTAAACCCTGGCGATCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGGTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCata                                                                                                                           <  1:228503/150‑1 (MQ=255)
                             ctcATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataa                                                                                                                          >  2:795862/1‑150 (MQ=255)
                              tcatcaCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataat                                                                                                                         <  1:709735/150‑1 (MQ=255)
                              tcATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTAACCATTCCATTTTTCataata                                                                                                                        >  2:1380468/1‑150 (MQ=255)
                               cATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataata                                                                                                                        >  2:563855/1‑150 (MQ=255)
                                  cccGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATAtta                                                                                                                     >  1:84255/1‑149 (MQ=255)
                                     ggCGCTTAAACCCTGGCGTTCGATCGGAAATCTGATTACGCTTATCGTGGGTATCCTGTGCGTTTCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTGATAATATTGAtt                                                                                                                  <  2:1484002/150‑1 (MQ=255)
                                      gcgcTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCAttt                                                                                                                 <  2:113244/150‑1 (MQ=255)
                                         cTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCATTAGCTAATATCAGTTGTGGATGGATCAAAATTGCAACAATTTTGAACCATTACATTTTTCATAATATTAATTTGtt                                                                                                              >  1:399547/1‑150 (MQ=255)
                                            aaaCCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTCGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAGTATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTATTCATAATATTAATTTGttatt                                                                                                           >  2:1435263/1‑150 (MQ=255)
                                               cccTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTattatt                                                                                                        >  2:876115/1‑150 (MQ=255)
                                                ccTTGCGTTCCATCTGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTc                                                                                                       <  1:714887/150‑1 (MQ=255)
                                                ccTGGCGTTCCATCGGAAATATGATTACGCTGATCGTTGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTc                                                                                                       <  1:697362/150‑1 (MQ=255)
                                                 cTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCATGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTcc                                                                                                      >  1:709956/1‑150 (MQ=255)
                                                  tGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCa                                                                                                     >  2:1702540/1‑150 (MQ=255)
                                                   ggCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCAc                                                                                                    >  1:426869/1‑150 (MQ=255)
                                                     cGTTCCATCGTAAATCTGATTACGCTGATCTTGGGTATCCTGTGCGTATCTGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCAccc                                                                                                  <  1:276138/150‑1 (MQ=255)
                                                           aTCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTACTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCAAACCTATCAc                                                                                            >  2:449883/1‑150 (MQ=255)
                                                            tCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACt                                                                                           <  1:1342917/150‑1 (MQ=255)
                                                            tCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAAAGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATAACt                                                                                           >  1:114218/1‑150 (MQ=255)
                                                             cGGAAATCTTATTACGCTGATCTTGGGTATCCTTTTCGTATCGGTAATGTTCTTTAGCTAATATCAGTTTTGGATGGTTCAAAATTGCTACAATTTTGAACGATTACATTTTTCATAATATTCATTTGTTATTATTCAACCCTATCACtt                                                                                          >  2:17682/1‑150 (MQ=255)
                                                             cGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACtt                                                                                          <  1:1542928/150‑1 (MQ=255)
                                                              ggAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGAATAGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAATTTGAACCATTCCATTTTTAATAATATAAATTTTTAATAATAACACCATATCACTtc                                                                                         >  1:381313/1‑150 (MQ=255)
                                                                aaaTCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtctc                                                                                       >  2:621543/1‑150 (MQ=255)
                                                                 aaTCTGATTACGCTGATCGTGGGTATCCTGTGCGTATAGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtcgct                                                                                      <  2:1159524/150‑1 (MQ=255)
                                                                   tCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCg                                                                                    <  1:1251334/150‑1 (MQ=255)
                                                                   tCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCg                                                                                    <  2:1443740/150‑1 (MQ=255)
                                                                    cTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGt                                                                                   <  1:802528/150‑1 (MQ=255)
                                                                    cTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGt                                                                                   <  1:436820/150‑1 (MQ=255)
                                                                    cTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGt                                                                                   <  2:1573593/150‑1 (MQ=255)
                                                                    cTGATTACGCTGAACGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGt                                                                                   <  1:1150055/150‑1 (MQ=255)
                                                                        ttACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCata                                                                               <  2:850923/150‑1 (MQ=255)
                                                                        ttACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCata                                                                               <  2:1054950/150‑1 (MQ=255)
                                                                        ttACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCata                                                                               <  2:228346/150‑1 (MQ=255)
                                                                        ttACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCata                                                                               <  1:60919/150‑1 (MQ=255)
                                                                              tGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAg                                                                         <  2:1024591/150‑1 (MQ=255)
                                                                                     gggTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGtattata                                                                  >  1:1660516/1‑149 (MQ=255)
                                                                                     gggTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATc                                                                  <  2:223487/150‑1 (MQ=255)
                                                                                         aTCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagtt                                                              <  2:797658/150‑1 (MQ=255)
                                                                                            cTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATAACCTAGTATAATCagttagt                                                           >  2:900759/1‑150 (MQ=255)
                                                                                              gtgCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAgttagtta                                                         <  1:1333965/150‑1 (MQ=255)
                                                                                                gCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTaaa                                                       <  1:866062/150‑1 (MQ=255)
                                                                                                    aTCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAacac                                                    <  2:635829/149‑1 (MQ=255)
                                                                                                    aTCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAacac                                                    >  1:635829/1‑149 (MQ=255)
                                                                                                      cGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAAcacaca                                                 <  1:64534/150‑1 (MQ=255)
                                                                                                      cGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGATCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAAcacaca                                                 >  2:1003460/1‑150 (MQ=255)
                                                                                                       ggTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCATAGTATTATCAGTTAGTTAAAAACACaa                                                >  1:773182/1‑150 (MQ=255)
                                                                                                           aTGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAagag                                            >  1:576377/1‑150 (MQ=255)
                                                                                                              ttCTTTAGCTAATATCAGTTGTGTATGTTTCAAAATTGATACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATc                                         <  1:1467400/150‑1 (MQ=255)
                                                                                                              ttCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATc                                         <  2:46879/150‑1 (MQ=255)
                                                                                                              ttCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATc                                         <  2:1598355/150‑1 (MQ=255)
                                                                                                               tCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCt                                        <  2:65400/150‑1 (MQ=255)
                                                                                                               tCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTAATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCt                                        <  2:130130/150‑1 (MQ=255)
                                                                                                                cTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTg                                       <  2:1637368/150‑1 (MQ=255)
                                                                                                                cTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTg                                       >  2:243243/1‑150 (MQ=255)
                                                                                                                   tAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTTGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAAc                                    <  1:1702540/150‑1 (MQ=255)
                                                                                                                    aGCTAATATCAGTTGTGGATGTTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACa                                   <  1:438214/150‑1 (MQ=255)
                                                                                                                      cTAATATCAGTTGTTGTTGGTTAAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATc                                 <  1:1529847/150‑1 (MQ=255)
                                                                                                                         atatCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTa                              <  2:581846/150‑1 (MQ=255)
                                                                                                                         atatCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTa                              <  2:1531100/150‑1 (MQ=255)
                                                                                                                           atCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTaaa                            <  2:1646561/150‑1 (MQ=255)
                                                                                                                           atCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTaaa                            >  2:53668/1‑150 (MQ=255)
                                                                                                                            tCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAAt                           >  2:1466481/1‑150 (MQ=255)
                                                                                                                              aGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCAAGTATTATCAGTTAGTTAAACACACAAAGATATCTGAACAACTTAAATaa                         >  2:592320/1‑150 (MQ=255)
                                                                                                                               gTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATaac                        >  2:1385056/1‑149 (MQ=255)
                                                                                                                                 tgtgTATTGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCAACCTATCAATTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGAc                      <  1:1184601/150‑1 (MQ=255)
                                                                                                                                 tgtgGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGAc                      <  2:699744/150‑1 (MQ=255)
                                                                                                                                     gATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTTGTTAAACACACAAAGAGATCTGAACATCTTATATAAGACTAtt                  >  2:1126167/1‑150 (MQ=255)
                                                                                                                                     gATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTAtt                  <  2:621315/150‑1 (MQ=255)
                                                                                                                                       tGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTa                <  1:1412295/150‑1 (MQ=255)
                                                                                                                                         gTTCAAAATTGTAGCAATTTTTAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCATATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAATAGATCTGAACATATTAAATAATACTATTTAAg              >  2:1571284/1‑150 (MQ=255)
                                                                                                                                         gTTCAAAATTGTAGCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAg              >  1:907406/1‑150 (MQ=255)
                                                                                                                                         gTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAg              >  1:1115693/1‑150 (MQ=255)
                                                                                                                                          ttCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAGTCTTATTTAAGa             <  2:576377/150‑1 (MQ=255)
                                                                                                                                           tCAAAATTGCTACAATTTTGCACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGAt            >  1:908946/1‑150 (MQ=255)
                                                                                                                                           tCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAGTCTTATTTAAGAt            <  1:876115/150‑1 (MQ=255)
                                                                                                                                           tCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGAt            <  1:621543/150‑1 (MQ=255)
                                                                                                                                             aaaaTTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGc          <  1:1606769/150‑1 (MQ=255)
                                                                                                                                               aaTTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAGTCTTATTTAAGATgttc        <  1:179025/150‑4 (MQ=255)
                                                                                                                                                 ttGCTACAATTTTGAACCATTCCATTTTTCATAATATTAATTTGTTATTATTCCACCCTATCTCTTCTCTCGTCATATACTAGTATTATCAGTTAGTTAAACACAAAAAGAGATCTGAACATCTTAAATAGTCTTATTTAAGATgttcag      <  2:1241793/150‑6 (MQ=255)
                                                                                                                                                    cTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTAGTCATATCCTAGTATTATCAGTTATTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGAATAACtt   >  1:288620/1‑150 (MQ=255)
                                                                                                                                                     tACAATTTTGAACCATTCCATTTTTCATAATATTAATTTGTTATTATTCCACCCTATCAATTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACTTa  <  2:243942/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACTTA  >  NC_000913/2986955‑2987253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: