Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,988,208 G→A 5.1% intergenic (+40/‑294) yqeH → / → yqeI putative LuxR family transcriptional regulator/putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,988,2080GA 5.1% 464.7 / 8.6 157intergenic (+40/‑294)yqeH/yqeIputative LuxR family transcriptional regulator/putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (83/62);  new base A (5/3);  total (89/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

Only 100 of 160 total aligned reads displayed.

TTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGTGGCATAAATGTTAACCATGTTAA  >  NC_000913/2988059‑2988354
                                                                                                                                                     |                                                                                                                                                  
ctacggatatccatatCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACggg                                                                                                                                                    >  1:712887/5‑150 (MQ=255)
ctacggatatccatatCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACggg                                                                                                                                                    >  1:1266258/5‑150 (MQ=255)
  cTGGATATGGATATACGTAGCATCTTCGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCAAACATTGATAGTCAACGACCAGCCAGAATAATACTCTGTTTATACGGGCa                                                                                                                                                  >  1:1063251/1‑150 (MQ=255)
   tggatatggataTCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGACAt                                                                                                                                                 >  2:1352643/1‑150 (MQ=255)
    ggatatggatatCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCAtt                                                                                                                                                >  2:31335/1‑150 (MQ=255)
         tgtatatCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTg                                                                                                                                           <  1:386784/147‑1 (MQ=255)
         tggatatCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCTGTCACACATTGATACTCAACGACCAGCCAGAATCATACTCTTTTTATACGGGCATTTATTg                                                                                                                                           >  1:549106/1‑150 (MQ=255)
          ggatatCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGa                                                                                                                                          <  2:136592/150‑1 (MQ=255)
           gatatCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGaa                                                                                                                                         <  2:387617/150‑1 (MQ=255)
           gatatCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGaa                                                                                                                                         >  1:1021666/1‑150 (MQ=255)
                 cGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTcc                                                                                                                                   <  2:90438/150‑1 (MQ=255)
                 cGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTcc                                                                                                                                   <  1:814060/150‑1 (MQ=255)
                  gTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCg                                                                                                                                  >  2:66167/1‑150 (MQ=255)
                  gTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCg                                                                                                                                  >  2:1340115/1‑150 (MQ=255)
                  gTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCg                                                                                                                                  <  2:1359882/150‑1 (MQ=255)
                    aGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGACATTTCAATAAATGCCCGTa                                                                                                                                <  1:623944/150‑1 (MQ=255)
                       aTCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGTCATTTATTGAAATGTCCGTAttt                                                                                                                             >  1:1376717/1‑150 (MQ=255)
                           aCGCGTACAAGCAACGAATCGAAAAGAGAATGGGTTGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTtata                                                                                                                         <  1:1719777/150‑1 (MQ=255)
                              cgTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATTTCCGTATTTTATAAAt                                                                                                                      >  2:522150/1‑150 (MQ=255)
                               gTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATa                                                                                                                     <  2:765540/150‑1 (MQ=255)
                                 aCAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAgg                                                                                                                   <  2:640165/150‑1 (MQ=255)
                                  cAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGc                                                                                                                  >  2:1172984/1‑150 (MQ=255)
                                   aaGCAACGAATCGAAAAGAGATTGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAggac                                                                                                                 >  1:147201/1‑148 (MQ=255)
                                    aGCAACGAATCGAAAAGATATTGGGTGGTAAAATAAACGAATTATTTATTCGTTCAAATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGTCATTTATTGAAATGTCCGTATTTTATAAATAGGCCg                                                                                                                <  2:1278741/150‑1 (MQ=255)
                                              cGAAAAGAGAATGGGTGGTAAAATAAACGAATTTTTTATTCGTTCACATTCGGTCCAACATTGATACTCATCGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAag                                                                                                      <  2:113745/150‑1 (MQ=255)
                                                aaaaGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAagag                                                                                                    >  1:1351470/1‑150 (MQ=255)
                                                aaaaGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAagag                                                                                                    >  1:1089377/1‑150 (MQ=255)
                                                 aaaGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAATTAGGCCGTCCCCTCAAGAGt                                                                                                   >  2:39065/1‑150 (MQ=255)
                                                 aaaGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGt                                                                                                   <  1:613801/150‑1 (MQ=255)
                                                   agagAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTaa                                                                                                 <  2:1021666/150‑1 (MQ=255)
                                                    gagaATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTaaa                                                                                                >  2:1316286/1‑150 (MQ=255)
                                                     agaATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAac                                                                                               <  1:122584/150‑1 (MQ=255)
                                                      gaATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAaca                                                                                              <  2:1001924/150‑1 (MQ=255)
                                                        aTGGGTGGTAAACTAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAATCAcc                                                                                            <  2:284346/150‑1 (MQ=255)
                                                           ggtggtAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATTCTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATa                                                                                         >  2:1512655/1‑150 (MQ=255)
                                                             tggtAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGt                                                                                       >  1:1599273/1‑150 (MQ=255)
                                                                  aaaTAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGACGTCACCTCAAGAGTAAACACCATAGTATTTa                                                                                  >  1:1375167/1‑150 (MQ=255)
                                                                     tAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTaaaa                                                                               <  1:861710/150‑1 (MQ=255)
                                                                     tAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACAAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCAGTACCCTCAAGAGTAAACACCATAGAATTTaaaa                                                                               >  1:1435674/1‑150 (MQ=255)
                                                                         cGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCAt                                                                           >  2:1571947/1‑150 (MQ=255)
                                                                             ttatttatTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATcaca                                                                       >  2:1279373/1‑150 (MQ=255)
                                                                             ttatttatTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATcaca                                                                       <  1:1385546/150‑1 (MQ=255)
                                                                                 ttattCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGaa                                                                   <  2:304945/150‑1 (MQ=255)
                                                                                  tattCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGTCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGaaa                                                                  <  1:182731/150‑1 (MQ=255)
                                                                                  tattCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGaaa                                                                  <  1:31335/150‑1 (MQ=255)
                                                                                  tattCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAAAGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGaaa                                                                  >  1:640075/1‑150 (MQ=255)
                                                                                       gTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTc                                                             <  1:1637764/150‑1 (MQ=255)
                                                                                        ttCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCa                                                            >  2:667574/1‑150 (MQ=255)
                                                                                          cacaTTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGa                                                          >  2:1227447/1‑150 (MQ=255)
                                                                                           acaTTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGaa                                                         >  2:75619/1‑150 (MQ=255)
                                                                                            caTTCGGTCCAACATTGATACTCAACGACCAGCCATAATCATACTCTGTTTATAAGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAg                                                        >  2:1103401/1‑150 (MQ=255)
                                                                                             aTTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATATTATTTAAAAACATCAAACGAAAAATTCAGAAGc                                                       >  1:232123/1‑150 (MQ=255)
                                                                                                cGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGGCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCAtt                                                    <  1:1036526/150‑1 (MQ=255)
                                                                                                 ggTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTa                                                   >  1:451714/1‑150 (MQ=255)
                                                                                                  gTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTAc                                                  >  1:1540685/1‑150 (MQ=255)
                                                                                                  gTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTAc                                                  <  1:422232/150‑1 (MQ=255)
                                                                                                   tCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACg                                                 <  1:1360913/150‑1 (MQ=255)
                                                                                                   tCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACg                                                 >  2:568684/1‑150 (MQ=255)
                                                                                                    ccAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGa                                                >  2:1549523/1‑150 (MQ=255)
                                                                                                     cAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGaa                                               >  1:1048620/1‑150 (MQ=255)
                                                                                                     cAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGaa                                               >  1:1502952/1‑150 (MQ=255)
                                                                                                      aaCATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACCCCATAGTATTTAAAACCATCACACGAAAAATTAAGAAGCATTACGaaa                                              >  2:210947/1‑150 (MQ=255)
                                                                                                      aaCATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGaaa                                              <  2:178590/150‑1 (MQ=255)
                                                                                                       aCATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAat                                             <  2:1707550/150‑1 (MQ=255)
                                                                                                        cATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAata                                            >  2:1248833/1‑150 (MQ=255)
                                                                                                             aTACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAAt                                       >  1:476626/1‑150 (MQ=255)
                                                                                                             aTACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACCAAAAATTCAGAAGCATTACGAAATATGAAt                                       >  2:1146004/1‑150 (MQ=255)
                                                                                                              tACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAACATTACGAAATATGAAtt                                      >  1:510611/1‑150 (MQ=255)
                                                                                                               aCTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAAttt                                     <  2:1048535/150‑1 (MQ=255)
                                                                                                               aCTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAAAATGAAttt                                     <  2:1499003/150‑1 (MQ=255)
                                                                                                                cacAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATATATAGAAATGACCGTAATTTATAAATAGGCCGTCCCCTCAAGATTAAACACCAAAGTATTAAAAACCATCACACGAAAAATACAGAAGAATAACGAAATATGAATTTa                                    >  1:1719727/3‑150 (MQ=255)
                                                                                                                cTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTa                                    <  2:1330873/150‑1 (MQ=255)
                                                                                                                  cAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGACCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTaca                                  <  1:1324246/150‑1 (MQ=255)
                                                                                                                   aaCGACCATCCAGAATCATACTCTGTTTATACGTTCATTTATTGAAATGTCAGTATTTTATAAATAGGCCGTCCCATCAAGACTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTacaa                                 <  1:251711/150‑1 (MQ=255)
                                                                                                                   aaCGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTacaa                                 >  2:440501/1‑150 (MQ=255)
                                                                                                                     cGACCAGCCAGAATCCTACTCTGTTTATACGGGCCTTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTacaaac                               <  2:85104/150‑1 (MQ=255)
                                                                                                                       aCCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTacaaacaa                             >  1:972476/1‑150 (MQ=255)
                                                                                                                       aCCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTacaaacaa                             >  2:813666/1‑150 (MQ=255)
                                                                                                                         cagccagAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCAAACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATa                           >  1:331045/1‑150 (MQ=255)
                                                                                                                         cagccagAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCAGTATATTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATa                           <  1:120817/150‑1 (MQ=255)
                                                                                                                          agccagAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAg                          >  2:590304/1‑150 (MQ=255)
                                                                                                                           gccagAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGt                         >  1:1515957/1‑150 (MQ=255)
                                                                                                                             cagAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGTgg                       >  1:1712311/1‑150 (MQ=255)
                                                                                                                             cagAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGTgg                       <  2:1456884/150‑1 (MQ=255)
                                                                                                                             cagAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGTgg                       >  1:977132/1‑150 (MQ=255)
                                                                                                                             cagAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATAAGAATTTACAAACAATAGTgg                       <  2:1171429/150‑1 (MQ=255)
                                                                                                                              agAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGTGGc                      <  1:1690473/150‑1 (MQ=255)
                                                                                                                                   cATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCTTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTAAGAAGCATTACGAAATATGAATTTACAAACAATAGTGGCATaaa                 >  2:1463035/1‑150 (MQ=255)
                                                                                                                                       ctctGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGTGGCATAAATGtt             >  1:1333097/1‑150 (MQ=255)
                                                                                                                                       ctctGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGTGGCATAAATGtt             >  1:1325940/1‑150 (MQ=255)
                                                                                                                                        ttataaaataCGGACATTTCAATAAATGCCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAGCCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGTGGCATAAATGTTa            >  1:755831/8‑150 (MQ=255)
                                                                                                                                        tctGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCTTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTAAGAAGCATTACGAAATATGAATTTACAAACAAAAGTGGCATAAATGTTa            >  2:696197/1‑150 (MQ=255)
                                                                                                                                         tataaaataCGGACATTTCAATAAATGCACGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATACAGAAGCATTACGAAATATGAATTTACAAACAATAGTGGCATAAATGTTaa           >  2:1017199/7‑150 (MQ=255)
                                                                                                                                           gTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGTGGCATAAATGTTAAcc         >  2:1610666/1‑150 (MQ=255)
                                                                                                                                              aataCGGACATTTCAATAAATGCCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGTGGCATAAATGTTAACCATg      >  1:1081661/2‑150 (MQ=255)
                                                                                                                                               ataCGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGTGGCATAAATGTTAACCATGt     <  2:1637361/150‑1 (MQ=255)
                                                                                                                                                taCTGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGTGGCATAAATGTTAACCATGtt    <  1:1153861/150‑1 (MQ=255)
                                                                                                                                                taCGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACAATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGTGGCTTAAATGTTAACCATGtt    >  2:138332/1‑150 (MQ=255)
                                                                                                                                                 aCGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGTGGCATAAATGTTAACCATGTTa   <  2:1204226/150‑1 (MQ=255)
                                                                                                                                                  cGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGTGGCATAAATGTTAACCATGTTaa  >  2:1041750/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACACGAAAAATTCAGAAGCATTACGAAATATGAATTTACAAACAATAGTGGCATAAATGTTAACCATGTTAA  >  NC_000913/2988059‑2988354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: