Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,993,896 T→A 5.2% intergenic (+40/‑43) ygeI → / → pbl uncharacterized protein/pseudogene, peptidoglycan‑binding enzyme family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,993,8960TA 5.2% 459.9 / 11.1 175intergenic (+40/‑43)ygeI/pbluncharacterized protein/pseudogene, peptidoglycan‑binding enzyme family
Reads supporting (aligned to +/- strand):  ref base T (82/84);  new base A (7/2);  total (89/86)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.54e-01

Only 100 of 184 total aligned reads displayed.

ATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTT  >  NC_000913/2993747‑2994045
                                                                                                                                                     |                                                                                                                                                     
  aCAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAg                                                                                                                                                     <  1:1391057/150‑1 (MQ=255)
   cAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGt                                                                                                                                                    >  2:1110876/1‑150 (MQ=255)
    aGATGATATAGAAGCATTTATAGTTTCAACATTGATGGCTCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAATATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTa                                                                                                                                                   <  1:108821/150‑1 (MQ=255)
     gATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTAt                                                                                                                                                  <  2:617973/150‑1 (MQ=255)
       tCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTAtct                                                                                                                                                <  2:457597/150‑1 (MQ=255)
          aaTACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGt                                                                                                                                             >  1:1353993/1‑150 (MQ=255)
           aTACAAGCATTTATAGTTTCTACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCAACTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTATTATAAAAGTATCTCGtt                                                                                                                                            <  2:479601/150‑1 (MQ=255)
            tACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTc                                                                                                                                           <  1:228824/150‑1 (MQ=255)
              cAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCt                                                                                                                                         >  2:640723/1‑150 (MQ=255)
              cAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCt                                                                                                                                         >  1:216241/1‑150 (MQ=255)
               aaGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCtt                                                                                                                                        >  1:1629539/1‑150 (MQ=255)
               aaGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCtt                                                                                                                                        >  1:632315/1‑150 (MQ=255)
                   aTTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcat                                                                                                                                    <  2:1135999/150‑1 (MQ=255)
                    tttATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcatt                                                                                                                                   <  2:783096/150‑1 (MQ=255)
                     ttATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcattc                                                                                                                                  <  2:318616/150‑1 (MQ=255)
                        taGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAAt                                                                                                                               <  1:139476/150‑1 (MQ=255)
                          ttttCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATcc                                                                                                                             <  1:787384/149‑1 (MQ=255)
                          gTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATcc                                                                                                                             <  1:936981/150‑1 (MQ=255)
                          atttCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATcc                                                                                                                             >  1:49835/2‑150 (MQ=255)
                            ttCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATATCGTTCCTTTCATTCAATCCTc                                                                                                                           >  1:792185/1‑150 (MQ=255)
                             tCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTTGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTca                                                                                                                          <  1:283704/150‑1 (MQ=255)
                             tCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCt                                                                                                                                         <  1:1163878/135‑1 (MQ=255)
                             tCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTca                                                                                                                          <  1:97399/150‑1 (MQ=255)
                                aCATTGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTcacac                                                                                                                       <  2:269084/150‑1 (MQ=255)
                                   tggatggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATg                                                                                                                    <  2:329294/150‑1 (MQ=255)
                                      atggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTcacac                                                                                                                       <  1:528060/144‑1 (MQ=255)
                                      atggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTcacac                                                                                                                       >  2:528060/1‑144 (MQ=255)
                                         gcgcCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGaaaaaa                                                                                                              >  2:543277/1‑150 (MQ=255)
                                          cgcCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATTAAAAAat                                                                                                             >  1:1068177/1‑150 (MQ=255)
                                           gcCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAata                                                                                                            >  1:430879/1‑150 (MQ=255)
                                            ccTTTTCAAAATTATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTAATAATAGTATCGCGTTCCTTTCATTCAATCCTCACACATGAAAAAatat                                                                                                           <  1:1323249/150‑1 (MQ=255)
                                               tttCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATgcg                                                                                                        >  2:1288335/1‑150 (MQ=255)
                                                ttCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATgcgc                                                                                                       <  2:1460018/150‑1 (MQ=255)
                                                  cAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTc                                                                                                     >  1:1688292/1‑150 (MQ=255)
                                                  cAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTc                                                                                                     >  2:222735/1‑150 (MQ=255)
                                                  cAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTGACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCAAAACGAGATACTTTTAAAATAGTAAGACGTTCATTTAATTCAATCATCAAACATGAAAAAATATGctcta                                                                                                     >  2:1509918/1‑149 (MQ=255)
                                                    aaaTGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATATCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCAc                                                                                                   >  1:1684795/1‑150 (MQ=255)
                                                          tatGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAg                                                                                             <  2:936497/150‑1 (MQ=255)
                                                          tatGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCACACACATGAAAAAATATGCGCTCACCAAAAg                                                                                             >  1:1270700/1‑150 (MQ=255)
                                                             gTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                                                                                          <  1:1028279/150‑1 (MQ=255)
                                                             gTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                                                                                          <  2:1215438/150‑1 (MQ=255)
                                                              tATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTaa                                                                                         >  1:1665684/1‑150 (MQ=255)
                                                               aTTCAGAAGATAATCATATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGATATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTaaa                                                                                        <  1:1377350/150‑1 (MQ=255)
                                                                 tCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAAtt                                                                                      >  2:889210/1‑150 (MQ=255)
                                                                  cAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTc                                                                                     >  2:1569751/1‑150 (MQ=255)
                                                                    taagtTAATACTATCTCACCTTTCTAAAAAATTGTGTGGTAGTAGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCtt                                                                                   <  1:1423849/149‑1 (MQ=255)
                                                                     aagaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTa                                                                                  <  1:1280401/150‑1 (MQ=255)
                                                                     aagaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTa                                                                                  >  1:158909/1‑150 (MQ=255)
                                                                      agaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTaa                                                                                 >  1:478343/1‑150 (MQ=255)
                                                                          aaTCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATATCGTTCATTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCAt                                                                             >  1:47036/1‑150 (MQ=255)
                                                                           aTCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATc                                                                            >  1:839511/1‑150 (MQ=255)
                                                                                aTCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCAc                                                                       <  2:609470/150‑1 (MQ=255)
                                                                                aTCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAAAGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCAc                                                                       <  2:112711/150‑1 (MQ=255)
                                                                                 tctcACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACg                                                                      <  1:1135210/150‑1 (MQ=255)
                                                                                 actcACCTTACTATAAAATTGAGTCGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACg                                                                      <  2:1594251/149‑1 (MQ=255)
                                                                                    cACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGGTCACCAAAAGTTAAATTCTTAACCATCTTCACGttt                                                                   >  2:139224/1‑150 (MQ=255)
                                                                                      ccTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTg                                                                 <  1:11592/150‑1 (MQ=255)
                                                                                           cTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCGAAAGTTAAATTCTTAACCATCTTCACGTTTTGTatat                                                            >  1:1004089/1‑150 (MQ=255)
                                                                                           cTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTatat                                                            >  2:1702553/1‑150 (MQ=255)
                                                                                             ataAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTAtattt                                                          <  1:666079/150‑1 (MQ=255)
                                                                                             ataAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTAtattt                                                          <  2:1715062/150‑1 (MQ=255)
                                                                                             ataAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTAtattt                                                          >  1:1220856/1‑150 (MQ=255)
                                                                                             ataAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTATGTAtattt                                                          >  2:364785/1‑150 (MQ=255)
                                                                                               aaaaTTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTAtatttat                                                        >  2:1190604/1‑150 (MQ=255)
                                                                                               aaaaTTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTAtatttat                                                        >  2:1682153/1‑150 (MQ=255)
                                                                                               aaaaTTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTAtatttat                                                        <  2:492082/150‑1 (MQ=255)
                                                                                                  aTTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTAc                                                     <  2:1166933/150‑1 (MQ=255)
                                                                                                       gTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACAAAAAGTTAAATTCTTAACCATCTTCACTTTTTGTATATTTATTACaaaaa                                                >  1:266452/1‑150 (MQ=255)
                                                                                                         ggTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACAAAGTAACGGTTTTTTATATTTTTTACAAAAAtt                                              >  2:1008097/1‑149 (MQ=255)
                                                                                                         ggTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTAACAAAAAGTTAAAATATTAACAATCTACACGTTTTGTATATTTATTACAAAAAtt                                              >  1:136098/1‑149 (MQ=255)
                                                                                                            aGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCt                                           >  2:1434486/1‑150 (MQ=255)
                                                                                                            aGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCt                                           >  2:267256/1‑150 (MQ=255)
                                                                                                                  ttGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTAGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGcc                                     <  1:342950/150‑1 (MQ=255)
                                                                                                                    gACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGTTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAg                                   <  1:130246/150‑1 (MQ=255)
                                                                                                                        aaataaatCATAACGAGATACTTTTATAATAGTATCTCGTTCATATCATTCAATCCTCACACATCAAAAAATATGCACTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAAc                               >  2:44639/1‑150 (MQ=255)
                                                                                                                         aataaatCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAAct                              <  2:859271/150‑1 (MQ=255)
                                                                                                                         aataaatCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTAACCAAAAGTTAAATTCTTAACCATCTTAACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAAct                              >  1:1472020/1‑150 (MQ=255)
                                                                                                                              atCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGc                         >  2:508027/1‑150 (MQ=255)
                                                                                                                              atCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGc                         <  1:1110876/150‑1 (MQ=255)
                                                                                                                              atCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGc                         >  1:1005244/1‑150 (MQ=255)
                                                                                                                              atCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCAAGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGc                         >  1:887345/1‑150 (MQ=255)
                                                                                                                                 ggaaCGAGATACTATTATAAAAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGctct                      >  2:232815/3‑150 (MQ=255)
                                                                                                                                  tAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTa                     <  2:891657/150‑1 (MQ=255)
                                                                                                                                  gaaCGAGATACTATTATAAAAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTa                     >  1:724697/2‑150 (MQ=255)
                                                                                                                                    aCGAGATACTATTATAAAAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACATAAATGTCTTTCGCCAGCAACTCTTGCTCTAAt                   >  1:393775/1‑150 (MQ=255)
                                                                                                                                    aCGAGATACTATTATAAAAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAAt                   >  1:152369/1‑150 (MQ=255)
                                                                                                                                     cGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATg                  <  2:1382328/150‑1 (MQ=255)
                                                                                                                                     cGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATg                  <  1:1309078/150‑1 (MQ=255)
                                                                                                                                     cGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATg                  <  2:309385/150‑1 (MQ=255)
                                                                                                                                     cGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCACTAATg                  >  1:849798/1‑150 (MQ=255)
                                                                                                                                     cGAGATACTATTATAAAAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATg                  >  2:545387/1‑150 (MQ=255)
                                                                                                                                      gagaTACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGa                 <  2:880594/150‑1 (MQ=255)
                                                                                                                                          tACTTTTATAATAGTATCTCGTTCCTTTCATTCAATACTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTATTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCa             <  1:16257/150‑1 (MQ=255)
                                                                                                                                           aCTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAg            <  2:1637358/150‑1 (MQ=255)
                                                                                                                                               ttATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAAc        <  1:543277/150‑1 (MQ=255)
                                                                                                                                                tataATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATTTTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACa       >  2:69880/1‑150 (MQ=255)
                                                                                                                                                   aataGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATg    <  1:325560/150‑1 (MQ=255)
                                                                                                                                                    ataGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATTTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGt   >  1:684825/1‑150 (MQ=255)
                                                                                                                                                    ataGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGt   >  2:956461/1‑150 (MQ=255)
                                                                                                                                                     taGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGtt  >  2:1122800/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTT  >  NC_000913/2993747‑2994045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: