Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,038,281 G→C 5.8% A181G (GCC→GGC)  dsbC ← protein disulfide isomerase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,038,2810GC 5.8% 402.8 / 11.2 121A181G (GCC→GGC) dsbCprotein disulfide isomerase II
Reads supporting (aligned to +/- strand):  ref base G (59/55);  new base C (3/4);  total (62/59)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 125 total aligned reads displayed.

GGTGTTCGTCGAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGATCCCCAGCGCGTTG  >  NC_000913/3038135‑3038430
                                                                                                                                                  |                                                                                                                                                     
 gtgtTCGTCGAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAActggctgg                                                                                                                                                   <  2:883192/150‑1 (MQ=255)
  tgtTCGTCGAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGt                                                                                                                                                  >  2:789757/1‑150 (MQ=255)
   gtTCGTCGAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTg                                                                                                                                                 <  1:1644828/150‑1 (MQ=255)
    ttCGTCGAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGc                                                                                                                                                <  1:1727323/150‑1 (MQ=255)
        tcgAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCACCAGCCAGTTGCGACg                                                                                                                                            <  2:563501/150‑1 (MQ=255)
        tcgAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCACCAGCCAGTTGCGACg                                                                                                                                            <  1:353507/150‑1 (MQ=255)
        gcgAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACg                                                                                                                                            <  1:652960/149‑1 (MQ=255)
          gAGGCATTCTTTCATCTCTTTCGGCGGCTGGTACCCCGGACCAAGGGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACgtg                                                                                                                                          <  1:307357/150‑3 (MQ=255)
          gAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCACCAGCCAGTTGCGACgtg                                                                                                                                          <  1:115448/150‑3 (MQ=255)
          gAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCt                                                                                                                                          >  1:1199720/1‑150 (MQ=255)
           aGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCCGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCTACTGGCTGGTGCGCCGCtt                                                                                                                                         >  1:1489854/1‑150 (MQ=255)
           aGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCACCAGCCAGTTGCGACgtgg                                                                                                                                         <  2:1403495/150‑4 (MQ=255)
           aGGAATTCTATCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGATCAGCACAACTGCCGGATTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGGCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCtt                                                                                                                                         <  2:1350094/150‑1 (MQ=255)
            ggAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCttt                                                                                                                                        >  2:76495/1‑150 (MQ=255)
            ggAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCttt                                                                                                                                        >  2:1347175/1‑150 (MQ=255)
                ttCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTAcc                                                                                                                                    <  2:1647961/150‑1 (MQ=255)
                ttCTTTCATCTCTTTCGGCGCCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACTCCAAGCTGGACGCCAAGTGCGTAATGGTCGCCATTATCCACGGCGCAACTGGCTGGTGCGACGCTTTTAcc                                                                                                                                    <  1:82110/150‑1 (MQ=255)
                  cTTTCATCTGATTCGGCGGGTTGTAACCCGGAACAAGTGTGCCATTGCACAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGTTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTg                                                                                                                                  <  2:251529/150‑1 (MQ=255)
                  cTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTg                                                                                                                                  >  2:852213/1‑150 (MQ=255)
                      cATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCcat                                                                                                                              >  2:744003/1‑150 (MQ=255)
                      cATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCcat                                                                                                                              >  2:40915/1‑150 (MQ=255)
                       aTCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCcata                                                                                                                             >  1:1068978/1‑149 (MQ=255)
                             ttCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCcatcacatca                                                                                                                       <  1:952252/150‑1 (MQ=255)
                                 gcggcTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCaa                                                                                                                   >  2:536288/1‑150 (MQ=255)
                                   ggcTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAAc                                                                                                                 >  2:1723610/1‑150 (MQ=255)
                                   ggcTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAAc                                                                                                                 <  2:1553916/150‑1 (MQ=255)
                                     cTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGc                                                                                                               >  1:247897/1‑150 (MQ=255)
                                       ggTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCtt                                                                                                             <  1:793699/150‑1 (MQ=255)
                                       ggTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCtt                                                                                                             <  1:1657174/150‑1 (MQ=255)
                                         tAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTg                                                                                                           >  1:611536/1‑150 (MQ=255)
                                          aaCCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGt                                                                                                          >  2:1392658/1‑150 (MQ=255)
                                           aCCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCCCGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGtt                                                                                                         >  2:450097/1‑150 (MQ=255)
                                            cccGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATTATCAAACGCTTTGttt                                                                                                        >  2:549026/1‑150 (MQ=255)
                                             ccGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGtgtt                                                                                                       >  1:753829/1‑150 (MQ=255)
                                              cGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGttttt                                                                                                      <  2:1492900/150‑1 (MQ=255)
                                                gAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGGCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCACACGCTTTGTTTTTAt                                                                                                    <  1:1514278/150‑1 (MQ=255)
                                                 aaCAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATc                                                                                                   <  2:1592155/150‑1 (MQ=255)
                                                   cAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACTTCGCATCTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCtt                                                                                                 <  1:650502/150‑1 (MQ=255)
                                                    aaGTGGGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGGGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCttt                                                                                                <  1:1570438/150‑1 (MQ=255)
                                                     aGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGCCGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTc                                                                                               >  1:455474/1‑150 (MQ=255)
                                                     aGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTc                                                                                               >  2:1238491/1‑150 (MQ=255)
                                                     aGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTc                                                                                               <  2:852129/150‑1 (MQ=255)
                                                      gtgtGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCg                                                                                              <  2:501103/150‑1 (MQ=255)
                                                      gtgtGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCg                                                                                              <  2:590258/150‑1 (MQ=255)
                                                         ggCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGcac                                                                                           <  2:326063/149‑1 (MQ=255)
                                                          gCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGcaca                                                                                          >  1:1080560/1‑150 (MQ=255)
                                                          gCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGcaca                                                                                          >  1:1551048/1‑150 (MQ=255)
                                                          gCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGcaca                                                                                          >  1:363670/1‑150 (MQ=255)
                                                           ccATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCacac                                                                                         >  1:436346/1‑150 (MQ=255)
                                                           ccATTGCTCAGCACAACGGCCGGAGTACCGCTACCGCCAAGCTGGACGCCAAGTGCGTAATGGTCGCCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCCTCACATCCTCAAACGCTTTGTTTTTATCTGTCGCacac                                                                                         >  1:1376590/1‑150 (MQ=255)
                                                           ccATTGCTCAGCAAAACTTCTGGAGTACCGATAACGACAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATTTCCACGTCGCAAGTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCacac                                                                                         <  2:293754/150‑1 (MQ=255)
                                                           ccATTGATCAGCACAAGTGCCGGTGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGGCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCacac                                                                                         <  2:293752/150‑1 (MQ=255)
                                                             aTTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCa                                                                                       <  1:158936/150‑1 (MQ=255)
                                                              ttGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAg                                                                                      <  2:539975/150‑1 (MQ=255)
                                                                 cTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATa                                                                                   <  1:453951/150‑1 (MQ=255)
                                                                  tCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAg                                                                                  >  1:1689559/1‑150 (MQ=255)
                                                                   cAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGc                                                                                 <  1:186561/150‑1 (MQ=255)
                                                                       acaACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTc                                                                             <  2:414114/150‑1 (MQ=255)
                                                                        caACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCa                                                                            >  2:1210044/1‑150 (MQ=255)
                                                                        caACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCACCGACGCAACTGGCTCGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTGTGTTTTTATCTTTCGCTCTCCAGATAGCTTTCa                                                                            >  1:492510/1‑150 (MQ=255)
                                                                         aaCTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCAt                                                                           >  1:994727/1‑150 (MQ=255)
                                                                            tGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCTTACGCTTTGTTTTTATCTTTCGCACACCTGATAGCTTTCAtttc                                                                        <  2:177503/150‑1 (MQ=255)
                                                                               cGGAGTACAGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTTCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCAtttcttt                                                                     >  2:345003/1‑150 (MQ=255)
                                                                                ggAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCAtttctttc                                                                    >  1:1276665/1‑150 (MQ=255)
                                                                                     aCCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGc                                                               >  2:156652/1‑150 (MQ=255)
                                                                                      ccGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCa                                                              <  1:1172433/150‑1 (MQ=255)
                                                                                       cGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCAt                                                             <  1:76495/150‑1 (MQ=255)
                                                                                        gCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATc                                                            >  1:1600245/1‑150 (MQ=255)
                                                                                        gCTAACGCCAAGCTGGACGCCAAGTGCGTAAGGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCTAAGCTTTTACCTGCCATCACATCATCAAACGGTTTGTTTTTATCTTTCGCACACCAGATAGCTTTGCTTTCTTTCTCTGCATc                                                            <  2:1376590/150‑1 (MQ=255)
                                                                                         cTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCg                                                           >  2:173170/1‑150 (MQ=255)
                                                                                             cGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCAGCACATCATCAAACGCTTTGGTTTTATCTTTCGCTCACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGt                                                       >  2:336846/1‑150 (MQ=255)
                                                                                              gCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCAtttctttc                                                                    <  2:189687/136‑1 (MQ=255)
                                                                                              gCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCAtttctttc                                                                    >  1:189687/1‑136 (MQ=255)
                                                                                               ccAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTcc                                                     >  2:218199/1‑150 (MQ=255)
                                                                                                cAAGCGGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGAGACGCTTTTACCTGCCATCACATCATCAATCGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCa                                                    <  1:98737/150‑1 (MQ=255)
                                                                                                    cTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGccc                                                <  2:1384652/150‑1 (MQ=255)
                                                                                                    cTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGccc                                                >  2:1020650/1‑150 (MQ=255)
                                                                                                       gACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTg                                             <  2:1162009/150‑1 (MQ=255)
                                                                                                        aCGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGGAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACTCCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCGTgg                                            <  1:1152029/150‑1 (MQ=255)
                                                                                                        aCGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGCCGCTTTTACCTGCCATCACATCATCAAACGATTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTgg                                            >  2:841936/1‑150 (MQ=255)
                                                                                                            cAAGTGCGTAATGGTCGGCAATATCGACGTCGCTACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGcgcg                                        <  2:779889/150‑1 (MQ=255)
                                                                                                                 gCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGaaa                                   >  2:1407799/1‑150 (MQ=255)
                                                                                                                  cGTAATGGTCGGCAATATCCACGTCGCAACTGGCTTGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAg                                  <  2:1116754/150‑1 (MQ=255)
                                                                                                                  cGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAg                                  <  1:744003/150‑1 (MQ=255)
                                                                                                                   gTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGc                                 <  2:1080560/150‑1 (MQ=255)
                                                                                                                        ggTCGGCAATAACCACGTAGCAACTGGCTGGTGAGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTATTTATCTTTCGCACACCAGATTGCTTTCATTTATATCTCAGCATCACAGTCCAGCCCCTGGCGAGATAAAGCAAGAt                            >  2:165717/1‑150 (MQ=255)
                                                                                                                           cGGCAATATCCACGTCGCAATTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGTAAGCAAGATAAc                         >  1:1083555/1‑150 (MQ=255)
                                                                                                                            ggCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACg                        <  2:247897/150‑1 (MQ=255)
                                                                                                                             gCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGc                       >  1:749556/1‑150 (MQ=255)
                                                                                                                                  atCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAAAGCTTTGTTTTTATCTTTCGCACACCAGATATCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGt                  <  2:237114/150‑1 (MQ=255)
                                                                                                                                   tCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTg                 >  1:1625095/1‑150 (MQ=255)
                                                                                                                                   aaagcGTCGCACCAGCCAGTTGCGACGTTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTg                 >  1:678536/5‑150 (MQ=255)
                                                                                                                                   aaagcGTCGCACCAGCCAGTTGCGACGCTTTTACATGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGAACACCAGATAGCTTTCATTTCTTTCTCTGCATCGATGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTg                 >  2:156320/5‑150 (MQ=255)
                                                                                                                                    ccACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGa                <  2:611536/150‑1 (MQ=255)
                                                                                                                                    ccACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTATATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCCGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTgg                >  2:310892/1‑149 (MQ=255)
                                                                                                                                    aagcGTCGCACCAGCCAGTTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGa                >  2:585352/4‑150 (MQ=255)
                                                                                                                                           gCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCGTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGATCCCCAg         >  1:1106416/1‑150 (MQ=255)
                                                                                                                                            cAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGATCCCCAgc        >  2:786303/1‑150 (MQ=255)
                                                                                                                                                 ggctggTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGATCCCCAGCGCGtt   >  1:1425426/1‑150 (MQ=255)
                                                                                                                                                  gctggTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGATCCCCAGCGCGTTg  <  1:912096/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GGTGTTCGTCGAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGATCCCCAGCGCGTTG  >  NC_000913/3038135‑3038430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: