Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,038,285 G→T 5.8% P180T (CCA→ACA) ‡ dsbC ← protein disulfide isomerase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,038,2850GT 5.8% 371.7 / 8.2 121P180T (CCA→ACA) ‡dsbCprotein disulfide isomerase II
Reads supporting (aligned to +/- strand):  ref base G (59/55);  new base T (3/4);  total (62/59)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01

Only 100 of 129 total aligned reads displayed.

GTGTTCGTCGAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGATCCCCAGCGCGTTGTAGT  >  NC_000913/3038136‑3038434
                                                                                                                                                     |                                                                                                                                                     
gtgtTCGTCGAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAActggctgg                                                                                                                                                       <  2:883192/150‑1 (MQ=255)
 tgtTCGTCGAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGt                                                                                                                                                      >  2:789757/1‑150 (MQ=255)
  gtTCGTCGAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTg                                                                                                                                                     <  2:1620516/150‑1 (MQ=255)
   ttCGTCGAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGc                                                                                                                                                    <  1:1727323/150‑1 (MQ=255)
    tcgtcgAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCg                                                                                                                                                   <  2:384345/150‑1 (MQ=255)
      gtcgAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGAc                                                                                                                                                 >  1:1592155/1‑150 (MQ=255)
       tcgAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCACCAGCCAGTTGCGACg                                                                                                                                                <  1:353507/150‑1 (MQ=255)
       tcgAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCACCAGCCAGTTGCGACg                                                                                                                                                <  2:563501/150‑1 (MQ=255)
       gcgAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACg                                                                                                                                                <  1:652960/149‑1 (MQ=255)
         gAGGCATTCTTTCATCTCTTTCGGCGGCTGGTACCCCGGACCAAGGGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACgtg                                                                                                                                              <  1:307357/150‑3 (MQ=255)
         gAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCACCAGCCAGTTGCGACgtg                                                                                                                                              <  1:115448/150‑3 (MQ=255)
          aGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCACCAGCCAGTTGCGACgtgg                                                                                                                                             <  2:1403495/150‑4 (MQ=255)
          aGGAATTCTATCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGATCAGCACAACTGCCGGATTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGGCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCtt                                                                                                                                             <  2:1350094/150‑1 (MQ=255)
           ggAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCttt                                                                                                                                            >  2:76495/1‑150 (MQ=255)
           ggAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCttt                                                                                                                                            >  2:1347175/1‑150 (MQ=255)
               ttCTTTCATCTCTTTCGGCGCCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACTCCAAGCTGGACGCCAAGTGCGTAATGGTCGCCATTATCCACGGCGCAACTGGCTGGTGCGACGCTTTTAcc                                                                                                                                        <  1:82110/150‑1 (MQ=255)
                 cTTTCATCTGATTCGGCGGGTTGTAACCCGGAACAAGTGTGCCATTGCACAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGTTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTg                                                                                                                                      <  2:251529/150‑1 (MQ=255)
                 cTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTg                                                                                                                                      >  2:852213/1‑150 (MQ=255)
                 cTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCAAAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTg                                                                                                                                      >  1:1207727/1‑150 (MQ=255)
                     cATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCcat                                                                                                                                  >  2:40915/1‑150 (MQ=255)
                     cATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCcat                                                                                                                                  >  2:744003/1‑150 (MQ=255)
                      aTCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCcata                                                                                                                                 >  1:1068978/1‑149 (MQ=255)
                            ttCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCcatcacatca                                                                                                                           <  1:952252/150‑1 (MQ=255)
                                gcggcTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCaa                                                                                                                       >  2:536288/1‑150 (MQ=255)
                                  ggcTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAAc                                                                                                                     <  2:1553916/150‑1 (MQ=255)
                                  ggcTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAAc                                                                                                                     >  2:1723610/1‑150 (MQ=255)
                                     tGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCt                                                                                                                  <  1:1685829/150‑1 (MQ=255)
                                      ggTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCtt                                                                                                                 <  1:1657174/150‑1 (MQ=255)
                                        tAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTg                                                                                                               >  1:611536/1‑150 (MQ=255)
                                         aaCCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGt                                                                                                              >  2:1392658/1‑150 (MQ=255)
                                             cGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGttttt                                                                                                          <  2:1492900/150‑1 (MQ=255)
                                               gAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGGCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCACACGCTTTGTTTTTAt                                                                                                        <  1:1514278/150‑1 (MQ=255)
                                                aaCAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATc                                                                                                       <  2:1592155/150‑1 (MQ=255)
                                                  cAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACTTCGCATCTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCtt                                                                                                     <  1:650502/150‑1 (MQ=255)
                                                    aGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGCCGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTc                                                                                                   >  1:455474/1‑150 (MQ=255)
                                                    aGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTc                                                                                                   <  2:852129/150‑1 (MQ=255)
                                                    aGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTc                                                                                                   >  2:1238491/1‑150 (MQ=255)
                                                     gtgtGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCg                                                                                                  <  2:501103/150‑1 (MQ=255)
                                                        ggCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGcac                                                                                               <  2:326063/149‑1 (MQ=255)
                                                         gCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGcaca                                                                                              >  1:1080560/1‑150 (MQ=255)
                                                         gCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGcaca                                                                                              >  1:363670/1‑150 (MQ=255)
                                                         gCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGcaca                                                                                              >  1:1551048/1‑150 (MQ=255)
                                                          ccATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCacac                                                                                             >  1:436346/1‑150 (MQ=255)
                                                          ccATTGCTCAGCACAACGGCCGGAGTACCGCTACCGCCAAGCTGGACGCCAAGTGCGTAATGGTCGCCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCCTCACATCCTCAAACGCTTTGTTTTTATCTGTCGCacac                                                                                             >  1:1376590/1‑150 (MQ=255)
                                                          ccATTGATCAGCACAAGTGCCGGTGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGGCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCacac                                                                                             <  2:293752/150‑1 (MQ=255)
                                                            aTTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCa                                                                                           <  1:158936/150‑1 (MQ=255)
                                                             ttGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAg                                                                                          <  2:539975/150‑1 (MQ=255)
                                                                cTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATa                                                                                       <  1:453951/150‑1 (MQ=255)
                                                                  cAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGc                                                                                     <  1:186561/150‑1 (MQ=255)
                                                                    gCAAAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACCTCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCtt                                                                                   <  2:306478/150‑1 (MQ=255)
                                                                     cacaACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCttt                                                                                  >  1:1492868/1‑150 (MQ=255)
                                                                      acaACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTc                                                                                 <  2:414114/150‑1 (MQ=255)
                                                                       caACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCACCGACGCAACTGGCTCGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTGTGTTTTTATCTTTCGCTCTCCAGATAGCTTTCa                                                                                >  1:492510/1‑150 (MQ=255)
                                                                        aaCTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCAt                                                                               >  1:994727/1‑150 (MQ=255)
                                                                           tGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCTTACGCTTTGTTTTTATCTTTCGCACACCTGATAGCTTTCAtttc                                                                            <  2:177503/150‑1 (MQ=255)
                                                                           tGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCAtttc                                                                            <  1:198159/150‑1 (MQ=255)
                                                                               ggAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCAtttctttc                                                                        >  1:1276665/1‑150 (MQ=255)
                                                                                     ccGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGcc                                                                  >  2:1102844/1‑149 (MQ=255)
                                                                                     ccGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCa                                                                  <  1:1172433/150‑1 (MQ=255)
                                                                                      cGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCAt                                                                 <  1:76495/150‑1 (MQ=255)
                                                                                       gCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATc                                                                >  1:1600245/1‑150 (MQ=255)
                                                                                       gCTAACGCCAAGCTGGACGCCAAGTGCGTAAGGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCTAAGCTTTTACCTGCCATCACATCATCAAACGGTTTGTTTTTATCTTTCGCACACCAGATAGCTTTGCTTTCTTTCTCTGCATc                                                                <  2:1376590/150‑1 (MQ=255)
                                                                                        cTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCg                                                               >  2:173170/1‑150 (MQ=255)
                                                                                          aaCGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGCCGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATGTTTCGCACACCAGATAGCTTTAATTTCTTTCTCTGCATCGCt                                                             >  2:52818/1‑150 (MQ=255)
                                                                                           aCGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTg                                                            >  1:723827/1‑150 (MQ=255)
                                                                                            cGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCAGCACATCATCAAACGCTTTGGTTTTATCTTTCGCTCACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGt                                                           >  2:336846/1‑150 (MQ=255)
                                                                                             gCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCAtttctttc                                                                        >  1:189687/1‑136 (MQ=255)
                                                                                             gCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCAtttctttc                                                                        <  2:189687/136‑1 (MQ=255)
                                                                                              ccAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTcc                                                         >  2:218199/1‑150 (MQ=255)
                                                                                               cAAGCGGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGAGACGCTTTTACCTGCCATCACATCATCAATCGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCa                                                        <  1:98737/150‑1 (MQ=255)
                                                                                                   cTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGccc                                                    >  1:765309/1‑150 (MQ=255)
                                                                                                   cTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGccc                                                    >  2:1020650/1‑150 (MQ=255)
                                                                                                      gACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTg                                                 <  2:1162009/150‑1 (MQ=255)
                                                                                                       aCGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGGAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACTCCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCGTgg                                                <  1:1152029/150‑1 (MQ=255)
                                                                                                            aaGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCgg                                           <  1:984003/150‑1 (MQ=255)
                                                                                                                 cGTAATGGTCGGCAATATCCACGTCGCAACTGGCTTGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAg                                      <  2:1116754/150‑1 (MQ=255)
                                                                                                                 cGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAg                                      <  1:744003/150‑1 (MQ=255)
                                                                                                                 cGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAg                                      <  2:1392471/150‑1 (MQ=255)
                                                                                                                  gTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGc                                     >  2:564103/1‑150 (MQ=255)
                                                                                                                       ggTCGGCAATAACCACGTAGCAACTGGCTGGTGAGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTATTTATCTTTCGCACACCAGATTGCTTTCATTTATATCTCAGCATCACAGTCCAGCCCCTGGCGAGATAAAGCAAGAt                                >  2:165717/1‑150 (MQ=255)
                                                                                                                          cGGCAATATCCACGTCGCAATTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGTAAGCAAGATAAc                             >  1:1083555/1‑150 (MQ=255)
                                                                                                                           ggCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCCTCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACg                            >  2:836476/1‑150 (MQ=255)
                                                                                                                           ggCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACg                            <  2:247897/150‑1 (MQ=255)
                                                                                                                            gCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGc                           >  1:749556/1‑150 (MQ=255)
                                                                                                                                 atCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAAAGCTTTGTTTTTATCTTTCGCACACCAGATATCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGt                      <  2:237114/150‑1 (MQ=255)
                                                                                                                                  tCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTg                     >  1:1625095/1‑150 (MQ=255)
                                                                                                                                  aaagcGTCGCACCAGCCAGTTGCGACGCTTTTACATGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGAACACCAGATAGCTTTCATTTCTTTCTCTGCATCGATGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTg                     >  2:156320/5‑150 (MQ=255)
                                                                                                                                   ccACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGa                    <  2:611536/150‑1 (MQ=255)
                                                                                                                                   ccACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTGCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGa                    >  2:758311/1‑150 (MQ=255)
                                                                                                                                   ccACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTATATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCCGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTgg                    >  2:310892/1‑149 (MQ=255)
                                                                                                                                   aagcGTCGCACCAGCCAGTTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGa                    >  2:585352/4‑150 (MQ=255)
                                                                                                                                          gCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGATCCCCAg             >  2:1511255/1‑150 (MQ=255)
                                                                                                                                          gCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCGTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGATCCCCAg             >  1:1106416/1‑150 (MQ=255)
                                                                                                                                           cAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGATCCCCAgc            >  2:786303/1‑150 (MQ=255)
                                                                                                                                                ggctggTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGATCCCCAGCGCGtt       >  1:1425426/1‑150 (MQ=255)
                                                                                                                                                 gctggTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGATCCCCAGCGCGTTg      <  1:912096/150‑1 (MQ=255)
                                                                                                                                                  ctggTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGATCCCCAGCGCGTTGt     <  1:789757/150‑1 (MQ=255)
                                                                                                                                                     gTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGTGAAAGCAAGATAACGCACGGTGATCCACAGCGCGTTGTAGt  >  1:767392/1‑150 (MQ=255)
                                                                                                                                                     gTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGATCCCCAGCGCGTTGTAGt  <  2:1600692/150‑1 (MQ=255)
                                                                                                                                                     gTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGATCCCCAGCGCGTTGTAGt  <  1:1673667/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGTTCGTCGAGGAATTCTTTCATCTCTTTCGGCGGCTGGTAACCCGGAACAAGTGTGCCATTGCTCAGCACAACTGCCGGAGTACCGCTAACGCCAAGCTGGACGCCAAGTGCGTAATGGTCGGCAATATCCACGTCGCAACTGGCTGGTGCGACGCTTTTACCTGCCATCACATCATCAAACGCTTTGTTTTTATCTTTCGCACACCAGATAGCTTTCATTTCTTTCTCTGCATCGCTGTCCAGCCCCTGGCGCGGGAAAGCAAGATAACGCACGGTGATCCCCAGCGCGTTGTAGT  >  NC_000913/3038136‑3038434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: