Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,052,886 C→G 6.5% A211A (GCG→GCC ubiH ← 2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,052,8860CG 6.5% 395.3 / 12.6 119A211A (GCG→GCCubiH2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (65/46);  new base G (5/3);  total (70/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

CCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCAT  >  NC_000913/3052737‑3053034
                                                                                                                                                     |                                                                                                                                                    
ccAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATc                                                                                                                                                      <  1:801327/150‑1 (MQ=255)
  aGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACCCCTCGTCGCGCCGTTCCAGTGGATGCCACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGc                                                                                                                                                    >  1:74251/1‑150 (MQ=255)
           aaTTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGcc                                                                                                                                           <  1:435204/150‑1 (MQ=255)
            aTTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCa                                                                                                                                          >  1:993302/1‑150 (MQ=255)
             ttttCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAACACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGAGGGCCAt                                                                                                                                         <  2:711771/150‑1 (MQ=255)
              tttCCCAAGTCGCCAGCCAAAGGCCGACGGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATg                                                                                                                                        <  2:928337/150‑1 (MQ=255)
               ttCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCAAGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGCCATCGGCAACATCGCCCGCGGGCCATGt                                                                                                                                       >  2:10631/1‑150 (MQ=255)
                  ccAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATGGCCCGCTGGCGATGTTGc                                                                                                                                    <  1:524848/150‑1 (MQ=255)
                  ccAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGGTCCAGTGGATGACACCAGACCAGCGCACCGCGTCCGTCAGCCATCGGCCACCTCGCCCGCGGGCCAGGGTGc                                                                                                                                    >  1:1563560/1‑150 (MQ=255)
                   cAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGCGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCg                                                                                                                                   <  2:53581/150‑1 (MQ=255)
                   cAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCAGTTACAGTGGATGACACCAGACCAGCGAACAGCGTCCGACAGACATCGGCAACATCGCCAGCGGGCCATGTTGCg                                                                                                                                   >  2:492550/1‑150 (MQ=255)
                    aaGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGt                                                                                                                                  >  2:947828/1‑150 (MQ=255)
                     aGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGGCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTa                                                                                                                                 <  2:280378/150‑1 (MQ=255)
                     aGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGGCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATGGCCCGCTGGCGATGTTGccga                                                                                                                                 <  1:23343/150‑4 (MQ=255)
                      gTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATGGCCCGCTGGCGATGTTGccgat                                                                                                                                <  1:199988/150‑5 (MQ=255)
                         gccagccaAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGc                                                                                                                             >  1:1009473/1‑150 (MQ=255)
                           cagccaAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGt                                                                                                                           >  1:610071/1‑150 (MQ=255)
                           cagccaAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGt                                                                                                                           >  2:1292683/1‑150 (MQ=255)
                              ccaACGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACCACACCTCTTCGCTCCTTTCCAGTGGATGTCACCAGACCTGCGAACAGCGTCCGTCAGACATCGCCAACATCGCCAGCGGGCCATGTTGCGTAAACCGTTCa                                                                                                                        <  2:254395/150‑1 (MQ=255)
                                     ccGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGCCACCAGACCAGCGAACAGCGTCCGTCAGCCATCGGCACCATCGCCCGCGGGCCATGTTGCGTAAAGCGTTCAAACgcgc                                                                                                                 >  1:128379/1‑150 (MQ=255)
                                       gACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAgcgcgc                                                                                                               <  1:14678/150‑1 (MQ=255)
                                       gACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGAAACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAgcacgc                                                                                                               >  2:1191010/1‑150 (MQ=255)
                                        aCTGGAGTTCACGGGAATACTTCTCGTCACTCCACGACAAGACCTGTTCGCGCCGTTCCTGTGGATGACACCAGACCCGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGcc                                                                                                              <  2:1151544/150‑1 (MQ=255)
                                           ggAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCtt                                                                                                           >  2:600634/1‑150 (MQ=255)
                                           ggAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCtt                                                                                                           <  1:575424/150‑1 (MQ=255)
                                            gAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTc                                                                                                          <  2:146100/150‑1 (MQ=255)
                                             aGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCa                                                                                                         >  2:948067/1‑150 (MQ=255)
                                             aGTTCACGGCAAAACTTCTCGACACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCa                                                                                                         >  2:128170/1‑150 (MQ=255)
                                              gTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCAt                                                                                                        <  1:14005/150‑1 (MQ=255)
                                               ttCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATg                                                                                                       <  1:813269/150‑1 (MQ=255)
                                                tCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATgc                                                                                                      >  1:440517/1‑150 (MQ=255)
                                                tCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATgc                                                                                                      <  2:927526/150‑1 (MQ=255)
                                                   cGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCa                                                                                                   >  1:1021962/1‑150 (MQ=255)
                                                      cAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACg                                                                                                <  1:1256131/150‑1 (MQ=255)
                                                      cAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACg                                                                                                >  1:1304171/1‑150 (MQ=255)
                                                       aaaaCTTGTCGTCACTCCACCAAAACACCTCTTCGCGCCGTACCAGTGGTTGTCACCATACCAGCGAAGAGCGTCCGTCAGGAATCGTCAACATCGCCAGCGGGCGATGTTTCGTAAAGCGTTCAATAGCGCGCCCTTCATGCGCAACgg                                                                                               <  2:117322/150‑1 (MQ=255)
                                                       aaaaCTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACgg                                                                                               <  2:1500164/150‑1 (MQ=255)
                                                        aaaCTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGa                                                                                              >  2:1096797/1‑150 (MQ=255)
                                                        aaaCTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGa                                                                                              <  1:515781/150‑1 (MQ=255)
                                                        aaaCTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACAGCCCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCCTGCGCAACGGaa                                                                                             >  1:1393839/1‑150 (MQ=255)
                                                         aaCTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGaa                                                                                             >  2:698375/1‑150 (MQ=255)
                                                           cTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGt                                                                                           >  1:176031/1‑150 (MQ=255)
                                                             tctcGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAg                                                                                         <  1:1016103/150‑1 (MQ=255)
                                                                cGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCaa                                                                                      >  2:976874/1‑150 (MQ=255)
                                                                cGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCaa                                                                                      >  2:952417/1‑150 (MQ=255)
                                                                      tCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTgg                                                                                >  2:645641/1‑150 (MQ=255)
                                                                      tCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTgg                                                                                <  1:1502745/150‑1 (MQ=255)
                                                                       ccACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACGGCGTCCGGCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAACAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGc                                                                               >  1:640305/1‑150 (MQ=255)
                                                                        cACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTggta                                                                              >  2:1460611/1‑148 (MQ=255)
                                                                        cACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGGCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCATCGTTGGCa                                                                              <  2:1210802/150‑1 (MQ=255)
                                                                                cacCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGGCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAACGCGTTCAAAAGAGCGCCCTTCATGCGCAACGGATGTAGCAACGTTGGCAATCACGGc                                                                      >  2:121680/1‑150 (MQ=255)
                                                                                  ccTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCa                                                                    >  1:997812/1‑150 (MQ=255)
                                                                                  ccTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCa                                                                    >  1:803431/1‑150 (MQ=255)
                                                                                   ctctTCGCGCCGTTCCAGTGGATGACCCCAGACCAGCGAACCGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAg                                                                   >  1:324322/1‑150 (MQ=255)
                                                                                    tctTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAgt                                                                  <  2:610071/150‑1 (MQ=255)
                                                                                    tctTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAgt                                                                  <  1:1626549/150‑1 (MQ=255)
                                                                                           gcCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGt                                                           >  2:1462376/1‑150 (MQ=255)
                                                                                            ccGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTa                                                          <  2:552332/150‑1 (MQ=255)
                                                                                             cGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTaa                                                         <  1:1097937/150‑1 (MQ=255)
                                                                                              gTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAGGCGTTCAAAAGCGCGCCCTTCATGCGCACCGGAAGTAGCAACGTTGGCAATCCCGGCCAGTTGTTCGTAAg                                                        >  1:707970/1‑150 (MQ=255)
                                                                                               ttCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGACAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCCCGGCCAGTTGTTCGTAAgg                                                       >  2:882352/1‑150 (MQ=255)
                                                                                                  cAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTc                                                    >  2:741560/1‑150 (MQ=255)
                                                                                                  cAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTc                                                    >  1:1189615/1‑150 (MQ=255)
                                                                                                    gTGGATGCCACCAGACCAGCGAACACCGTCCGTCAGCCATCGGCAACATCGCCAGAGCTCCATGTTGCGTAAAGCGTTCAAAACCGCGCCCTTCATGCGCAACGGAAGTAGCCACGTAGTCAATCACGGCCAGTGGTTCGTAAGGCTCct                                                  >  2:699885/1‑150 (MQ=255)
                                                                                                    gTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCct                                                  <  1:503333/150‑1 (MQ=255)
                                                                                                     tGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCctg                                                 >  2:958131/1‑150 (MQ=255)
                                                                                                         tGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCtgctgc                                             <  1:948067/150‑1 (MQ=255)
                                                                                                         tGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCtgctgc                                             <  1:128170/150‑1 (MQ=255)
                                                                                                          gACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGcc                                            <  2:1382567/150‑1 (MQ=255)
                                                                                                           acacCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCa                                           <  1:1292683/150‑1 (MQ=255)
                                                                                                            cacCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAACGCGCGCCCTTCATGCGCTCCGGAAGTAGCAACGTTGGCAATCTCGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAg                                          >  2:740755/1‑150 (MQ=255)
                                                                                                            cacCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAg                                          >  2:932747/1‑150 (MQ=255)
                                                                                                              ccagcccagcGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAACGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTc                                        >  1:180242/1‑150 (MQ=255)
                                                                                                                agaccagCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCACCGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCaa                                      >  2:1531719/1‑150 (MQ=255)
                                                                                                                 gaccagCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAAc                                     >  2:448688/1‑150 (MQ=255)
                                                                                                                   ccagCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAAcgc                                   >  2:1288448/1‑150 (MQ=255)
                                                                                                                    cagCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAAcgcc                                  >  2:1099250/1‑150 (MQ=255)
                                                                                                                      gCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAAcgccgc                                <  1:234497/150‑1 (MQ=255)
                                                                                                                        gAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCAc                              <  1:1462376/150‑1 (MQ=255)
                                                                                                                          aCAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCGTGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCAcgc                            <  1:1495474/150‑1 (MQ=255)
                                                                                                                            aGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCgg                          >  2:1081430/1‑150 (MQ=255)
                                                                                                                             gCGTCCGGCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGt                         <  2:1417284/150‑1 (MQ=255)
                                                                                                                              cgtccgtcAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTg                        >  2:734904/1‑150 (MQ=255)
                                                                                                                                  cgtcAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTa                    <  2:1304171/150‑1 (MQ=255)
                                                                                                                                    tcAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAAc                  <  2:1724853/150‑1 (MQ=255)
                                                                                                                                     cAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCCCGGCCAGTTGTTCGTCAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACg                 >  2:308147/1‑150 (MQ=255)
                                                                                                                                      aGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGc                <  1:1193453/150‑1 (MQ=255)
                                                                                                                                         cATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGa             <  2:1563560/150‑1 (MQ=255)
                                                                                                                                         cATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGa             >  1:606700/1‑150 (MQ=255)
                                                                                                                                         cATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGa             <  2:74251/150‑1 (MQ=255)
                                                                                                                                           tCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAAt           >  2:1199194/1‑150 (MQ=255)
                                                                                                                                            cGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATg          >  2:1534051/1‑150 (MQ=255)
                                                                                                                                            cGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATg          <  1:1165795/150‑1 (MQ=255)
                                                                                                                                             cgCAACATGGCCCGCTGGCGATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATgg         >  2:1587498/2‑150 (MQ=255)
                                                                                                                                              gCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATggg        <  2:505148/150‑1 (MQ=255)
                                                                                                                                               cAACATGGCCCGCTGGCGATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGt       >  2:1307169/1‑150 (MQ=255)
                                                                                                                                                 aCATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGc     <  1:932747/150‑1 (MQ=255)
                                                                                                                                                  cATGGCCCGCTGGCGATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGcc    >  2:1710267/1‑150 (MQ=255)
                                                                                                                                                  cATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGcc    >  1:770386/1‑150 (MQ=255)
                                                                                                                                                    tCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCAt  >  2:826496/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCAT  >  NC_000913/3052737‑3053034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: