Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,052,893 G→T 6.5% P209Q (CCG→CAG)  ubiH ← 2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,052,8930GT 6.5% 373.3 / 10.9 122P209Q (CCG→CAG) ubiH2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (68/46);  new base T (5/3);  total (73/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

Only 100 of 127 total aligned reads displayed.

AATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCATCAGCTGCT  >  NC_000913/3052748‑3053042
                                                                                                                                                 |                                                                                                                                                     
aaTTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGcc                                                                                                                                                   <  1:435204/150‑1 (MQ=255)
 aTTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCa                                                                                                                                                  >  1:993302/1‑150 (MQ=255)
  ttttCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAACACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGAGGGCCAt                                                                                                                                                 <  2:711771/150‑1 (MQ=255)
    ttCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCAAGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGCCATCGGCAACATCGCCCGCGGGCCATGt                                                                                                                                               >  2:10631/1‑150 (MQ=255)
       ccAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATGGCCCGCTGGCGATGTTGc                                                                                                                                            <  1:524848/150‑1 (MQ=255)
       ccAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCGTGTTGc                                                                                                                                            >  2:1495474/1‑150 (MQ=255)
       ccAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGGTCCAGTGGATGACACCAGACCAGCGCACCGCGTCCGTCAGCCATCGGCCACCTCGCCCGCGGGCCAGGGTGc                                                                                                                                            >  1:1563560/1‑150 (MQ=255)
        cAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCAGTTACAGTGGATGACACCAGACCAGCGAACAGCGTCCGACAGACATCGGCAACATCGCCAGCGGGCCATGTTGCg                                                                                                                                           >  2:492550/1‑150 (MQ=255)
         aaGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGt                                                                                                                                          >  2:947828/1‑150 (MQ=255)
          aGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGGCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTa                                                                                                                                         <  2:280378/150‑1 (MQ=255)
           gTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATGGCCCGCTGGCGATGTTGccgat                                                                                                                                        <  1:199988/150‑5 (MQ=255)
              gccagccaAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGc                                                                                                                                     >  1:1009473/1‑150 (MQ=255)
                cagccaAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGt                                                                                                                                   >  1:610071/1‑150 (MQ=255)
                cagccaAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGt                                                                                                                                   >  2:1292683/1‑150 (MQ=255)
                  ggcaAAGGCCGACTGGTGTTCACGGCAAAACTTCTCGTCACTCCACGACATCACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTc                                                                                                                                 <  2:1609338/148‑1 (MQ=255)
                   ccaACGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACCACACCTCTTCGCTCCTTTCCAGTGGATGTCACCAGACCTGCGAACAGCGTCCGTCAGACATCGCCAACATCGCCAGCGGGCCATGTTGCGTAAACCGTTCa                                                                                                                                <  2:254395/150‑1 (MQ=255)
                          ccGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGCCACCAGACCAGCGAACAGCGTCCGTCAGCCATCGGCACCATCGCCCGCGGGCCATGTTGCGTAAAGCGTTCAAACgcgc                                                                                                                         >  1:128379/1‑150 (MQ=255)
                             aCTGGAGTTCACGGGAATACTTCTCGTCACTCCACGACAAGACCTGTTCGCGCCGTTCCTGTGGATGACACCAGACCCGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGcc                                                                                                                      <  2:1151544/150‑1 (MQ=255)
                                ggAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCtt                                                                                                                   >  2:600634/1‑150 (MQ=255)
                                ggAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCtt                                                                                                                   <  1:575424/150‑1 (MQ=255)
                                 gAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTc                                                                                                                  <  2:146100/150‑1 (MQ=255)
                                  aGTTCACGGCAAAACTTCTCGACACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCa                                                                                                                 >  2:128170/1‑150 (MQ=255)
                                   gTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCAt                                                                                                                <  1:14005/150‑1 (MQ=255)
                                     tCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATgc                                                                                                              >  1:440517/1‑150 (MQ=255)
                                     tCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATgc                                                                                                              <  2:927526/150‑1 (MQ=255)
                                      cACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATgcg                                                                                                             <  1:1463550/150‑1 (MQ=255)
                                        cGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCa                                                                                                           >  1:1021962/1‑150 (MQ=255)
                                         ggCAAAACTTCTCGTCACTCCCCGACAACACCTCTTCGCGCCGATCCAGTGGATCACACCAGCCCAACGCACAGCGTCCCTCAGAACTCGACAACATCGCCAGCGGGCCATGTTGCGAAAAGCGATCAAAAGCTCGACCATCATGAGCaa                                                                                                          >  1:81106/1‑150 (MQ=255)
                                           cAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACg                                                                                                        >  1:1304171/1‑150 (MQ=255)
                                           cAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACg                                                                                                        <  1:1256131/150‑1 (MQ=255)
                                            aaaaCTTGTCGTCACTCCACCAAAACACCTCTTCGCGCCGTACCAGTGGTTGTCACCATACCAGCGAAGAGCGTCCGTCAGGAATCGTCAACATCGCCAGCGGGCGATGTTTCGTAAAGCGTTCAATAGCGCGCCCTTCATGCGCAACgg                                                                                                       <  2:117322/150‑1 (MQ=255)
                                            aaaaCTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACgg                                                                                                       <  2:1500164/150‑1 (MQ=255)
                                             aaaCTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGa                                                                                                      >  2:1096797/1‑150 (MQ=255)
                                              aaCTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGaa                                                                                                     >  2:698375/1‑150 (MQ=255)
                                                cTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGt                                                                                                   >  1:176031/1‑150 (MQ=255)
                                                  tctcGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAg                                                                                                 <  1:1016103/150‑1 (MQ=255)
                                                     cGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCaa                                                                                              >  2:952417/1‑150 (MQ=255)
                                                     cGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCaa                                                                                              >  2:976874/1‑150 (MQ=255)
                                                        cACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAATGCGTTCAAAAGCGCGGCCTTCATGCGCAACGGAAGTAGCAACGt                                                                                           >  2:93353/1‑150 (MQ=255)
                                                           tCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTgg                                                                                        <  1:1502745/150‑1 (MQ=255)
                                                            ccACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACGGCGTCCGGCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAACAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGc                                                                                       >  1:640305/1‑150 (MQ=255)
                                                             cACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTggta                                                                                      >  2:1460611/1‑148 (MQ=255)
                                                             cACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGGCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCATCGTTGGCa                                                                                      <  2:1210802/150‑1 (MQ=255)
                                                                 acaacaCCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCa                                                                                  >  2:1165795/1‑150 (MQ=255)
                                                                    acacCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACgg                                                                               >  2:709249/1‑150 (MQ=255)
                                                                     cacCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGGCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAACGCGTTCAAAAGAGCGCCCTTCATGCGCAACGGATGTAGCAACGTTGGCAATCACGGc                                                                              >  2:121680/1‑150 (MQ=255)
                                                                       ccTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCa                                                                            >  1:803431/1‑150 (MQ=255)
                                                                        ctctTCGCGCCGTTCCAGTGGATGACCCCAGACCAGCGAACCGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAg                                                                           >  1:324322/1‑150 (MQ=255)
                                                                         tctTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAgt                                                                          <  2:610071/150‑1 (MQ=255)
                                                                                 ccGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTa                                                                  <  2:552332/150‑1 (MQ=255)
                                                                                  cGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTaa                                                                 <  1:1097937/150‑1 (MQ=255)
                                                                                    ttCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGACAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCCCGGCCAGTTGTTCGTAAgg                                                               >  2:882352/1‑150 (MQ=255)
                                                                                       cAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTc                                                            >  2:741560/1‑150 (MQ=255)
                                                                                       cAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTc                                                            >  1:1189615/1‑150 (MQ=255)
                                                                                        aGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTcc                                                           <  1:1288640/150‑1 (MQ=255)
                                                                                         gTGGATGCCACCAGACCAGCGAACACCGTCCGTCAGCCATCGGCAACATCGCCAGAGCTCCATGTTGCGTAAAGCGTTCAAAACCGCGCCCTTCATGCGCAACGGAAGTAGCCACGTAGTCAATCACGGCCAGTGGTTCGTAAGGCTCct                                                          >  2:699885/1‑150 (MQ=255)
                                                                                         gTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCct                                                          <  1:503333/150‑1 (MQ=255)
                                                                                          tGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCctg                                                         >  2:958131/1‑150 (MQ=255)
                                                                                             aTGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTACAAAAGCGCGCCCTTCAAGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCctgctg                                                      >  1:648382/1‑150 (MQ=255)
                                                                                              tGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCtgctgc                                                     <  1:948067/150‑1 (MQ=255)
                                                                                                acacCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCa                                                   <  1:1292683/150‑1 (MQ=255)
                                                                                                 cacCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAACGCGCGCCCTTCATGCGCTCCGGAAGTAGCAACGTTGGCAATCTCGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAg                                                  >  2:740755/1‑150 (MQ=255)
                                                                                                 cacCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAg                                                  >  2:932747/1‑150 (MQ=255)
                                                                                                  accagaccagCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGt                                                 >  1:1174710/1‑150 (MQ=255)
                                                                                                   ccagcccagcGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAACGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTc                                                >  1:180242/1‑150 (MQ=255)
                                                                                                     agaccagCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCACCGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCaa                                              >  2:1531719/1‑150 (MQ=255)
                                                                                                      gaccagCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAAc                                             >  2:448688/1‑150 (MQ=255)
                                                                                                          agCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAAcgccg                                         >  2:1370725/1‑150 (MQ=255)
                                                                                                           gCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAAcgccgc                                        <  1:234497/150‑1 (MQ=255)
                                                                                                             gAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCAc                                      <  1:1462376/150‑1 (MQ=255)
                                                                                                               aCAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCGTGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCAcgc                                    <  1:1495474/150‑1 (MQ=255)
                                                                                                                 aGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCgg                                  >  2:1081430/1‑150 (MQ=255)
                                                                                                                  gCGTCCGGCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGt                                 <  2:1417284/150‑1 (MQ=255)
                                                                                                                      ccgtcAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCt                             >  1:1670801/1‑150 (MQ=255)
                                                                                                                       cgtcAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTa                            <  2:1304171/150‑1 (MQ=255)
                                                                                                                         tcAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAAc                          <  2:1724853/150‑1 (MQ=255)
                                                                                                                          cAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCCCGGCCAGTTGTTCGTCAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACg                         >  2:308147/1‑150 (MQ=255)
                                                                                                                           aGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGc                        <  1:1193453/150‑1 (MQ=255)
                                                                                                                              cATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGa                     <  2:74251/150‑1 (MQ=255)
                                                                                                                              cATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGa                     >  1:271/1‑150 (MQ=255)
                                                                                                                                tCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAAt                   >  2:1199194/1‑150 (MQ=255)
                                                                                                                                 cGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATg                  >  2:1534051/1‑150 (MQ=255)
                                                                                                                                 acgCAACATGGCCCGCTGGCGATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATg                  >  1:517711/3‑150 (MQ=255)
                                                                                                                                  ggCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATgg                 <  2:1021962/150‑1 (MQ=255)
                                                                                                                                  cgCAACATGGCCCGCTGGCGATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATgg                 >  2:1587498/2‑150 (MQ=255)
                                                                                                                                   gCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATggg                <  2:505148/150‑1 (MQ=255)
                                                                                                                                    cAACATGGCCCGCTGGCGATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGt               >  2:1307169/1‑150 (MQ=255)
                                                                                                                                    cAACATGGCCCGCTGGCGATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGt               >  2:585057/1‑150 (MQ=255)
                                                                                                                                      aCATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGc             <  1:932747/150‑1 (MQ=255)
                                                                                                                                       cATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGcc            >  1:770386/1‑150 (MQ=255)
                                                                                                                                         tCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCAt          >  2:826496/1‑150 (MQ=255)
                                                                                                                                           gCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCATCa        >  1:660994/1‑150 (MQ=255)
                                                                                                                                             cAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCATCAgc      >  2:1606192/1‑150 (MQ=255)
                                                                                                                                             cAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCATCAgc      >  2:1674170/1‑150 (MQ=255)
                                                                                                                                              aGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCATCAgct     <  1:1567231/150‑1 (MQ=255)
                                                                                                                                                 gggCCATGTTGCGTAACGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGGTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCATCAgctgct  >  2:934989/1‑150 (MQ=255)
                                                                                                                                                 gggCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCATCAgctgct  <  2:803431/150‑1 (MQ=255)
                                                                                                                                                 gggCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCATCAgctgct  <  1:357151/150‑1 (MQ=255)
                                                                                                                                                 gggCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCATCAgctgct  >  1:530989/1‑150 (MQ=255)
                                                                                                                                                 gggCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGACAATCACGGCCACTTGTTCGTAAGGCTCCTGCTGACAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCATCAgctgct  >  1:97414/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
AATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCATCAGCTGCT  >  NC_000913/3052748‑3053042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑